Fluorescence live cell movie analyzer - VWR

Copyright 2013 @ NanoEnTek Inc. All rights reserved Fluorescence Live cell movie analyzer

Fluorescence live cell movie analyzer

Understanding cellular growth, behavior and morphology

Copyright 2013 @ NanoEnTek Inc. All rights reserved Fluorescence Live cell movie analyzer 2

About

The JuLI™ FL, is the next generation of a smart fluorescence live cell movie

analyzer & counter.

The JuLI™ FL uses state-of-the-art optics to get live-cell images from

various cell culture dishes and can be performed cell-based assays, including

cell counting, cell viability and GFP/RFP expression level checking.

This also enables user to support the quantified cell confluence results with low

variation and the growth curve using image based analysis for bright field.


Appearance (Whole body)


Appearance (Scopes)

For GFP channel

For RFP channel


Dual system (*Optional) Allow to compare the control and experimental

samples using dual system.

5

Compatible to CO2 incubator


Versatility (Flexibility)

Features

Time-lapse image capturing and movie making

Cell counting and viability

GFP or RFP expression level checking

Dual system for comparative data (* optional)

Accuracy

Automated confluence detection & growth curve

Semi-Auto focusing with low variation

Ease of use

Intuitive & user friendly interface with LCD touch screen

Compact size optimized for an cell culture incubator

Simple steps for the system setup and the operations

Live cell

imaging Cell counting

& viability Expression

level check

Dual system

Confluence detection

Compact

for incubator User friendly

Real time

growth curve


Live cell imaging (time lapse)

Cell growth monitoring

Cell migration assay

Cell confluence detection

Cell culture quality control

Proliferation assay

Stem cell development

Cell-based assay optimization

Cell counting & Viability

Transfection efficiency (GFP or RFP Expression)

Applications


Applications

Cell confluence detection

Cell confluence determined various cell lines

Real-time cell growth curve

NIH-3T3 cells growth were observed 50 hours through JuLI™ Br. All images were recorded every 10 minute

and analyzed monolayer confluence.


Applications

Time-lapse image capture and movie making

Cell-growth images were captured for 63 hours with 20 minutes intervals in U-2 OS GFP & RFP stable cell line.

Live cell image capture

U-S OS cells were transfected using the Neon™ transfection system (from Life Technologies) and 0.25 µg of the

the EGFP-N1 plasmid & RFP plasmid. 48 hours after transfection, the images are captured.


Applications

Cell migration (wound healing) assay

U-2OS stable cells were incubated for 100 hours after scratch. JuLITM FL calculated the confluence with growth

of surface unfarmed automatically.

Bright field

Wound confluence can be graphed to

quantitatively analysis the recovering

the surface of wound.

(Bright field only)


Applications

Cell counting & viability

Clumped cell (HepG2, MCF7, F9) cells were counted with a JuLITM FL, a hemocytometer and the competitor’s automated

cell counter. Accuracy and precision are comparable between the JuLITM FL and the hemocytometer for all cell lines.

Cell counting results were performed on three different instruments with seven sample replicates

Fluorescence expression level checking

JuLITM FL counting software provides actual cell counts, viability and automatically calculates cell concentration.

It also can identify and measure the levels of fluorescence (GFP or RFP expression).

Transfection efficiency (A) Fluorescence intensity gating (B)

GFP expressing cells (U-2 OS) were counted using JuLITM FL counting software (A). The histograms in

graph (B) show the green fluorescence intensity of the cells.


Software description

Focusing menu Monitoring menu

Data menu Setting menu


Cell-growth images were captured for 63 hours with 20

minutes intervals in U-2 OS GFP stable cell line.

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Movie data

U-2 OS GFP stable cells proliferations

Cell-growth images were captured for 63 hours with 20

minutes intervals in U-2 OS RFP stable cell line.

U-2 OS GFP stable cells proliferations

http://youtu.be/1EnTch0TouM

http://youtu.be/JoWjjLlU54M






Movie data

Cell migration (wound healing) assay

U-2OS stable cells were incubated for 100 hours after

scratch. JuLITM FL calculated the confluence with growth

of surface unfarmed automatically.

http://youtu.be/k-zqhFI6nuw






Specification

Magnification 4 X and digital zoom ( ~ 450 X)

Image resolution 1280 x 960 pixels (1.3 M)

Exported formats JPEG, TIFF, BMP, PNG (image), AVI (movie), CSV (raw data)

Display 10.1” LCD touch screen

Light source GFP channel (JULI-FLG04): Blue LED

RFP channel (JULI-FLR04): Green LED

Optical filter GFP channel (JULI-FLG04): Excitation 466/40 nm, Emission 525/50 nm

RFP channel (JULI-FLR04): Excitation 525/50 nm, Emission 580LP

Dimension & Weight Scope unit : 300 x 190 x 188 mm, 4.5 kg

Station unit : 282 x 285 x 160 mm, 3.2 kg

Storage 320 GB Hard drive, 4 GB USB drive

Power AC100 - 240Va.c.,, 1.9A, 100w, 50/60 Hz

Operating Environment 5 - 40 °C, 20 – 95 %


Ordering information

Cat. No. Description Remark

Device

JULI-FLG04 JuLI FL (Single set)

For GFP channel JULI-FLGD04 JuLI FL (Dual set)

JULI-FGSC Scope unit of JuLI FL

JULI-FLR04 JuLI FL (Single set)

For RFP channel JULI-FLRD04 JuLI FL (Dual set)

JULI-FRSC Scope unit of JuLI FL

Accessories

JULI-BRCM Counting starter kit Counting module set, Cell counting slide

JULI-BRTB XY stage

Disposable

JULI-BRS50 Cell counting slide 50 slides (100 counts)

JULI-BRS1000 Cell counting slide 1,000 slides (2,000 counts)

JULI-BRS5000 Cell counting slide 5,000 slides (10,000 counts)


Appendix_Magnification

Field of view comparison

1.21 mm

0.92 mm

138.92 mm

104.19 mm

Field of view (Actual, Camera)

1.21 x 0.92 = 1.113 mm2

Field of view (Display, LCD Screen)

138.92 x 104.19 = 14474 mm2

Full size (X1) 114X

Zoom in (Digital X4) 228X

Zoom in (Digital X16) 456X

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Copyright 2013 @ NanoEnTek Inc. All rights reserved V.1.0 (Sept, 2013)

Fluorescence live cell movie analyzer

Website

www.nanoentek.com

E-mail

[email protected]

NanoEnTek Inc. (HQ)

12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 152-740, Korea

Tel : +82-2-6220-7940 / Fax : +82-2-6220-7721

NanoEnTek USA Inc.

5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA

Tel : +1-925-225-0108, +1-888-988-0108(Toll free)

Fax : +1-925-225-0109

http://www.nanoentek.com/

mailto:[email protected]


JuLI Br VS FL 기능적 개선 사항

•Capture and monitoring 시 다양한 image format: Br현재 (JPEG) à FL (JPEG, TIFF, BMP, PNG)로 확장 장점: 다양한 포멧 제공 가능, PC 베이스처럼 확장 함. 단점: 이미지 포멧에 따른 용량이 상이하게 차이 남 – data transfer 시 상당한 시간 소요됨

•모니터링 시 세팅 조건 변경: Br현재 (모니터링 한번 설정 하면 세팅 조건 변경 안됨) à 모니터링 시 일시 정지 후 Focus 탭으로 이동하여 Focus / Brightness / Exposure 재 조정 가능 장점: 몰폴로지 변화 등으로 초점이 나가거나 사용자가 세팅 조건 조정 가능 단점: Cell growth curve 그릴때 세팅 조건을 봐꾸면, 그래프가 연속되지 않고 출렁 거리거나, 끊어지는 것 처럼 보일 수 있음 (이는 사용자 조건 변화에 따른 것으로 complaint 아님)

Fluorescence live cell movie analyzer - VWR

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