Lipemic sample

Dr.M.P.Saravanan,MDAssociate professor

KAPV Government Medical college,trichy,Tamil Nadu

Fasting Lipemic & Normal serum

Lipemic Normal

Letters can be read thro’ clear serum

Letters not visible

Normal Lipemic

Letters visible through

serum

Serum not transparent milky white appearance

Lipemic Clear serum

EDTA SAMPLE SHOWING MILKY WHITE PLASMA

MILKY WHITE PLASMA

1/5 dilution of serum TGL & Chol values

1/20 DILUTION TRYGLYCERIDE

10,500 MG/DL

TOTAL CHOLESTEROL=1400 mg.dl

Sodium – 128 mEq/dl (pseudo hyponateremia)

Case history of this sample 43yr/M came to hospital with c/o

tiredeness,no h/o chest pain or any event of CAD in his family. Known diabetic

No alcohol intake in previous 3 days Repeated the sample next day with fasting Results remain the same

Why is lipemic sample important? Lipemic sample is a cloudy sample –

scatters light in colorimetry- assay error with all chemistries

Matrix effect- less water component in serum or plasma due to more solid(lipid)- less electrolyte estimation- pseudo hyponateremia in direct ISEs

Uncontrolled diabetes – where you get high values of TGL.

Congenital dyslipidemias- Go for Lipoprotein EPP

Look for cardiac risk- start dietary management & drugs if necessary