Life Science Summer Institute 2006

This project was funded through the San Diego Workforce This project was funded through the San Diego Workforce Partnership, Inc. by a grant awarded under the President's High Partnership, Inc. by a grant awarded under the President's High Growth Job Training Initiative, as implemented by the U.S. DeparGrowth Job Training Initiative, as implemented by the U.S. Department tment of Labor's Employment and Training Administration of Labor's Employment and Training Administration

What we love about LSSI:Participating in effective & rewarding professional growth

Having resources to implement biotechnology in the classroom

Showing students how this science relates to the “real world”

Working with talented teachers from throughout the county

What we love about LSSI:Learning about biotechnology techniques, industry needs, and cutting-edge science

Bringing “workable” biotech into the classroom

Discussing potential biotech and career paths for our students

Seeing the multi-disciplinary nature of biotechnology

Who was involved:

• SCBC at Miramar College: Sandy Slivka & Sharon Price

• Amgen/Bruce Wallace curriculum: Marty Ikkanda

• San Diego Workforce Partnership• BioCom• Biogen Idec Community Lab• 20 teachers from San Diego County

Week 1

• Safety• Intro to biotech industry• Research local biotech companies and

institutes• Identify California state standards

associated with biotechnology• Perform biotech labs• Bioethics discussion

Lab Safety Lab Safety -- PPEPPE(Personal Protective Equipment)(Personal Protective Equipment)

• Closed-toe shoes

• Pants• Lab coats• Safety glasses• Appropriate

gloves

Emergency Procedures

• KNOW the locations of your:

• Emergency exit and have back up ready if needed

• Staging Area• Intercom / Phone• First Aid kit and AED• Fire extinguisher• Eyewash/safety shower.

Lab 2 Restriction Digest

pARA & pKAN-R

Lab 1 IntroRecombinant Pathway

Lab 3 Ligation

Lab 4a Confirmation of Digestion

Lab 2a

pARA-R

Non-recombinant Pathway

Lab 4 Confirmation of Restriction & Ligation

Lab 5a Transformation

w. pARA-R

Lab 5 Transformation w.

Recombinant Plasmid

Lab 6 Culture of E. coli

Lab 7 mFPProtein

Purification

Lab Technologies

• Micropipetting• Gel Electrophoresis• Restriction Enzyme Digests• Plasmid Ligation• Bacterial Transformation-heat shocking• Cell Culture• Protein Purification• DNA Extraction

Weeks 2 & 3

Field trips to various biotech companies and research institutes.

Types of Exposure• Demonstration of utilization of Basic Science Concepts

in Applied Fields • Exploration of Drug Development Process • Overview of Company Structure/Function • Organizational/Department Lead Panel • Laboratory/Facility Tour • Scientist Panel • Scientist Presentation • Hands-on Demonstration • Career Exploration/Options • Information on Networking Events • Investor Relations/Communications

Genentec

San Diego Natural History Museum

Karl Strauss

Gen_Probe

The Burnham Institute

SALK

Pfizer

ISIS

Nanogen

Dow

Alexion / Accumetrics

CRES / Wild Animal Park

Invitrogen

Genoptix

Miramar College - SCBC

Biogen Idec

Summer 2006

Isis Pharmaceuticals

• Founded in 1989• Carlsbad, CA• Holds 1500+ patents• 250 employees• Research & development, small scale

manufacture of Vitravene drug

Sectors

• Medical/pharmacology sector–Antisense drugs (“Vitravene”)

• Industrial/environmental sector–Biosensors (“Ibis T5000 TIGER”)–Small molecule division

Antisense Drugs

• Target specific sequence of mRNA

• Block translation of mRNA into amino acid sequence

• Result = target protein can’t be made

http://www.biotech.ubc.ca/MolecularBiology/AntisenseRNA/antisense1.gif

Alexion“engaged in discovering and developing a portfolio of antibody therapeutics targeting severe unmet medical needs. AAT discovery efforts are focused on cancer, autoimmune disorders and infectious disease.”

– Antibody therapeutics focused on cancer, autoimmune disorders, and infectious diseases

• Thrombocytopenia (depleting of platelets due to chemotherapy

– Genetically altered antibody to increase production of platelets

Tools• Genetic Engineering

– Propriety assays– High throughput screening– Protein modification– Protein purification– Bacterial and mammalian expression of

antibodies and fragments

Tecan

“Tecanator”

Core Technology• CoALT – customized human antibody

libraries– Generation of highly specific, fully human antibodies– Rapid generation of fully human or humanized

antibodies– Facilitates a seamless transition from genomic

research to drug development– Direct sources of antibodies for therapies

Accumetrics“Mission” Statement

Accumetrics is a privately-held biotech company focused on the design, development, manufacturing, and marketing of innovative point-of-care cardiovascular diagnostic products

Products - Overviewoptimize the effectiveness of

antiplatelet therapies guide treatment decisions to

reduce risk of bleeding complications

Tools to Monitor Platelet function:

Advantages:easy, automated, rapid & accurate

Products - DetailsThe VerifyNow™ System consists of an instrument, disposable assays & controls.

• Instrument is an optical detection system which measures platelet induced aggregation (~$5000 per instrument)

• Assays based on platelet receptor sites (Aspirin, P2Y12, and IIb/IIIa so far)(single-use $20-$50 each)

DOW San Diego

Dow: Protein of Interest

• Dow Pharma, Dow Agra send proteins to be expressed

• Use Pseudomonus fluorescens• Microliter to 1500 liter fermenters• Most proteins processed code for

enzymes• Human growth/antibodies…current focus

Dow: Amplify/Analyze

• Grow up in tubes• Analyze down to DNA

sequencing• Shakers to grow• Robotic device for

making cell preps• Billion cells per mL

• Protein expression• 2-d gels• Separate by size and

isoelectric points• pH gradient strips• Thin layer acrylimide• High voltage 8kV

Dow: Process

• PCR amplify• Modify• Express• Analyze/What works• Scale up• Ferment• Recover

Nanogen Corporate Headquarters10398 Pacific Center Court

San Diego, CA 92121

Mission• Nanogen's mission is to

become a leading supplier of advanced diagnostic solutions to the medical community and to clinical researchers.

• Multiple markets include: – research– clinical – point-of-care

• Technology integrates advanced microelectronics & molecular biology on proprietary semi-conductor microchips.

• Diagnostic Research Biotechnology

• A production & RD mature company

some recent news

• Nanogen Launches Cystic Fibrosis Analyte Specific Reagents 23 March 06specific reagents (ASRs) to detect 23 wild type and mutation sequences associated with cystic fibrosis (CF).

• Nanogen Announces Type II Diabetes Research Collaboration 15 May 06

• Acquires Italian Molecular Diagnostics Company Amplimedical 20 April 06

Accomplishments• >350 Patents & >250 Licensing Agreements• 17 Companies founded to develop Institute technologies

Research Directions• Chemistry and Proteomics• Stem Cell Biology• Cell Biology• Regulatory Biology• Metabolic Research• Computational and Theoretical Biology

A non-profit, research institute with:Three research centers:• NCI Cancer Center• Del E. Webb Center for Neurosciences and Aging• Infectious and Inflammatory Disease CenterThree technology centers:• San Diego Center for Chemical Genomics• Stem Cell Research Center• Center on Proteolytic Pathways

80% of funding is due to federal grants.

www.burnham.org

Tools and Technology

• Molecular Library Screening Network

• Stem-Cell “biologics-free” culture growth

• Gene array • DNA library

screenings• Molecular Biology

such as gene therapy

More Tools and Technology

• Signal transduction for proteases

• Microscopy• Molecular targeting• 3-D molecular maps• Monoclonal

Antibodies such as anti-cancer drugs

• And more . . .

Gen-Probe Incorporated10210 Genetic Center DriveSan Diego, CA 92121-4362

Employees: 1000 + International Hqtrs on Genetic Center Drive****80 Hours in 9 Days (10 hour days)

Stage of Growth: 3rd stage: Production and R&D - 23 years old

Background/History: Founded in 1983Five Core Technologies:1) Ribosomal RNA (rRNA)2) Target Capture3) Transcription-Mediated Amplification4) Hybridization Protection Assay (HPA)5) Dual Kinetic Assay (DKA)

Financial Status: Stock Share Price: $53.46 – 7/12/06 1600Z

.

Conclusion:

1. Diverse company with a niche in the diagnostic arena – both technical (equipment) and process (kits)

2. Established and thriving company

3. Good growth potential because of aggressive attitude toward applications (tests) and understanding of need (marketing strategy address social concern – HIV and Prostate Cancer)

4. Employment potential strong – variety of opportunities

a. legal, public affairs, technical in both lab and non-lab areas (I.T. and Biology)

Lunch with scientists at Gen-Probe

Karl Strauss:Industrial Biotechnology

Production of fermented beverages

LSSI Group at Karl Strauss

THE Scientist at Karl Strauss

Pfizerhttp://www.pfizer.com/pfizer/are/index.jsp

• Our Mission - We will become the world's most valued company to patients, customers, colleagues, investors, business partners, and the communities where we work and live.

• Our Purpose - We dedicate ourselves to humanity's quest for longer, healthier, happier lives through innovation in pharmaceutical, consumer, and animal health products.

Research Highlights @ Pfizer La Jolla

• Cancer• Goal - To improve patient survival rates primarily in

the area of novel agents targeting anti-angiogenesis, signal transduction, and cell cycle regulation.

• Viral Disease• Goal - To identify new antiviral therapies to treat

these serious viral diseases

• Diabetes & Obesity• Goal - Treatment of diabetes and obesity

specifically by suppressing appetite, increasing energy expenditure, sensitizing diabetic patients to the actions of insulin and treating diabetic complications

• Disease of the Eye• Goal - Seeking to identify and develop medications

that maintain vision and prevent blindness.• Specifically focusing on anti-angiogenesis

compounds to treat age-related macular degeneration, diabetic retinopathy, and diabetic macular edema.

Pfizer Core TechnologiesLarge corporate umbrellayields a broad spectrum oftechnologies:• Monoclonal antibodies• Molecular Biology

– Genetic Engineering– DNA Technology– Tracers

• Cell Culture

Employment

• 100,000 full-time global employees

• Job Openings– 100 globally– 48 in Oceanside

• Ranging from Supervisors, Scientists, Engineers, Interns, Human Resources, and Technical Trainers

Sector and Growth• Genentech works in the medical and

pharmaceutical sector.– Focuses on Oncology, Immunology, and

Tissue Growth and Repair• They are in several stages of growth for

their various products.– Development for some products.– Research for others.– Production and marketing for other

products.• The Oceanside site is a manufacturing site.

Tools• Recombinant DNA

processes• Oceanside site has

large scale equipment for manufacturing– Large Bio-reactors,

etc.

CRES

Sustainable Populations

Genotyping California Condors forpedigree analysis and genetic mapconstruction.

Development of non-invasive imaging techniques to detect pregnancy in Giant Pandas.

Maternal care strategies in GiantPandas and other bears.

Founded in 1987 and incorporated in 1989 with headquarters in Carlsbad, CA

Conducts business in more than 70 countries

25,000 products and services to support disease research, drug discovery, and commercial bio-production

Stage of Growth: Production and R&D

Invitrogen Corporation1600 Faraday AvenueCarlsbad, California 92008Phone: (760) 603-7200Fax: (760) 602-6500www.invitrogen.com

Applications Antibodies & ImmunodetectionBead–Based SeparationsBio-ProductionCell CultureCloning

DNA & RNA PurificationFlow CytometryGene Expression ApplicationsHLA DiagnosticsTransfection

Areas of FocusAvian Influenza

Vaccine BioproductionBiodefense

Infectious diseases and pathogen detectionBioinformatics

Vector software for PC & MacDrug Discoveries and Solutions

Kinases & Phosphatases, nuclear receptors, Ion Channels, G-Protein Coupled Receptors

Proteomics and Protein Expression Separation, Quantitation/Mass Spectrometry, Protein Expression

Stem Cell Research Isolation, Characterization, Expansion, Differentiation

Genoptix has developed a breadth of services that focus on the challenges of each phase of patient management to help in effective diagnosis, optimal treatment decisions and monitoring of hematopoietic diseases such as Leukemia, Lymphoma and a non-hemopoietic disease, Breast Cancer metastatic stage 4.

Technologies• Morphology and

Histopathology– Microscopic analysis

of tissues • 6 color flow cytometry

– Examination of single suspended cells using laser beam which gives information about size and markers on cells

• Cytogenetics– Looking at

chromosomes using karyotypes

• FISH (Fluorescence in situ Hybridization)– Examination of cell

abnormalities not requiring cells in division

• RT-PCR (Reverse Transcriptase Polymerase Chain Reaction)

COMPASS• Provides a clear, consistent summary

report that assimilates everything a physician needs to know for appropriate patient intervention in hematopoieticdisorders. A "one glance" summary report correlating all technologies for optimal diagnostic review, decision making and patient management

Diagnose TreatPredict Monitor

Corporate VisionCorporate Vision

With passion, purpose and partnerships, we transform scientific discoveries into

advances in human healthcare.

With passion, purpose and partnerships, we transform scientific discoveries into

advances in human healthcare.

Biotechnology Sector

• This company is involved with research, development, production, and sales of novel medical and pharmaceutical therapies in the fields of:– Oncology– Neurology– Immunology

Core Technology

Monoclonal Antibodies make up the core of their research

Thanks for the Hospitality!

Curriculumdevelopment

Networking withcolleagues

LSSI Week 4

Updating Sharon’scalendar

Teacher Presentationsof labs

LSSI Week 4

Camille Fowler

“This was a great opportunity to meet dedicated teachers, meet industry

professionals, and bring exciting technology to my classroom”

LSSI Week 4Teacher Personal

Statements

“My Chemistry students are really going to benefit from this experience.

I’m looking forward to integrating biotechnology ideas into our curriculum.”

Stephanie Gaudreau

LSSI Week 4Teacher Personal

Statements

Steve Bartram

“Biotech Rules! This is the greatest group ever!”

LSSI Week 4Teacher Personal

Statements