LSSI 2007

•This project was funded through the San Diego Workforce Partnership, Inc. by a grant awarded under the President's High Growth Job Training Initiative, as implemented by the U.S. Department of Labor's Employment and Training Administration

Amgen Bruce Wallace Biotechnology Lab Program

Lab 8Genomic DNA Extraction from Buccal

Epithelial Cells/PCR Analysis

Lab 7Purification of mFP from Overnight Culture

Lab 6Preparing an Overnight Culture of E. Coli

Lab 5Transforming E. coli with a

Recombinant Plasmid

Lab 4Confirmation of Restriction & LigationUsing Agarose Gel Electrophoresis

Lab 3Ligation of pARA & pKAN-R Restriction Fragments

Producing a Recombinant Plasmid, pARA-R

Lab 2Resitriction Analysis of pARA & pKAN-R

Lab 5aTransformation of E. coli with pARA-R

Lab 4aConfirmation of pARA-R Restriction Digest

Using Agarose Gel Electrophoresis

Lab 2apARA-R Restriction Digest:

An Introduction to Plasmids and Restriction Enzymes

Lab 1An Introduction to Volumetrics & Pipetting

As all humans, we want to feel that we are a part of a greater mission – Annie Glidden (Biogen idec)

Teacher Share-out: Taking it Back to the Classroom

1)Teachers shared laboratory activities and simulations for implementation in the classroom.

2)School-to-Career ApplicationA. Industry Guest Speakers (Scientists and

other Industry Personnel)B. Industry Site Visits and ToursC. Internship Opportunities

3)Sharing of available educational resources

High Schools:Temecula Valley Unified:Brett Baughman Great OakOceanside Unified:Christine Meza El CaminoTuyet-Mai Vo El CaminoVista Unified:Christi Newman VistaSan Marcos Unified:Matthew Leader High Tech North CountyEscondido Union:Nicole Hoofard EscondidoPoway Unified:Vicki Binswanger WestviewLisa Smedley Rancho BernardoMalinda Dixon WestviewSusanne Nelson PowaySan Diego Unified:Ellie Vandiver Crawford Andy Mangahis Patrick HenryLisa Yoneda Mira Mesa

High Schools (cont.):La Mesa:Paul Messier Helix CharterGrossmont Unified:Glenn Melero El Cajon ValleySteele Canyon:Betty Jensen Steele CanyonSweetwater Union High:Darci Kimball Castle ParkMike Sixtus Bonita VistaRoderick Warner Bonita VistaVictor Rodriguez SouthwestPrivate:Jamie Howard Lutheran

Colleges:San Diego Community College:Anar Brahmbhatt Ph. D. MesaTheresa Bolanos Ph.D. City Sandra Slivka Ph. D. MiramarLinda Woods Ph. D. MiramarSharon Price MiramarSouthwestern Community College:Nouna Bakhiet Ph. D. Southwestern

RECOMBINANT PIPETTE

pARA

MICROFUGE

E. COLI

pKAN

AGAROSE

MICROLITER

POLYMERASEENZYMELIGASEDNA

TAQ

PCR

PLASMID

RNA

BIOTECHNOLOGYCENTRIFUGE

KARL STRAUSS

CONATUS

PFIZERBIOGEN-IDEC

DOWPHARMA

INVITROGEN

GENENTECH

ESTUDY

LYSIS

SALK

HYDROPHOBICPERCIPITATE

PRIMER

ELUTE

HYDROPHILIC

RESTRICTION

BIOCOM

PROTEINTRANSFORMATION

ALUCENTRAL DOGMAAMINO ACID

AMGEN

PURIFY

DIGEST

ELECTROPHORESIS

BUCCAL

ALIQUOT

GENOMIC

mFPDIGEST

HPLC

SPECTROPHOTOMETER

BamH I

VOLTAGE

SB BUFFER

STICKY END

Hind III

CHO

California Standards & Amgen Lab Curriculum

1a-d, 1g, 1k-m

1a, 1m1aInvestigation & Experimentation

3cPhysics

2a, 2b, 2h2h, 5g, 8b, 8d,

10a

Chemistry

1b, 1h, 4a, 5a-e

1b, 5a-e1a-d, 4c, 5a-c, 10d

Biology

Lab 1: Lab 2: Lab 2a: Lab 3:

California Standards & Amgen Lab Curriculum

1a-d, 1f, 1g, 1j, 1k

1a-d, 1j, 1m

1a-c1a-e, 1j, 1i

Investigation & Experimentation

5a, 5c, 5ePhysics

8b2a, 6d, 10b

4d, 10aChemistry

1a, 1b, 5a-d, 10d

1a, 1c, 5c-e, 7a,

8a

1b, 1d, 5c-e

1h, 4c, 5a-e

Biology

Lab 4: Lab 4a: Lab 5: Lab 5a:

California Standards & Amgen Lab Curriculum

1a, 1b, 1d, 1m

1a, 1m1d, 1mInvestigation & Experimentation

5a, 5c3ePhysics

2a, 5g, 8b, 8c,

10a

3c, 10cChemistry

1a, 1b, 2e, 3a, 5c, 7a,

7b

1b, 1d, 1h, 4a-c, 4e, 5a-c,

10d

5c, 5eBiologyLab 6: Lab 7: Lab 8:

Biotech Helping Society:• Industrial and

Environmental– Fermented Foods and

Beverages• Medical and

Pharmaceutical– Antibiotics– Vaccines– Monoclonal Antibodies– Hormone Synthesis

• Diagnostic Research– PCR– ELISA– Sequencing

Good Work Ethic

Detail Oriented

Dependability

Good OrganizationSkills

Good CommunicationSkills

Good Problem-SolvingSkills

Safety Oriented

Ability to get alongwith others

Flexibility

Team Oriented

Fast Learner

We are the home depot of Biotech. We will not build anything for you, but we will give you everything

you need- Lisa Peterson (Invitrogen)

www.karlstrauss.comSan Diego, CA

KARL STRAUSS BREWERY

http://www.karlstrauss.com/

History

• 1989: Opened first Karl Strauss microbrewery in San Diego

• 1992: Opened main brewery on Santa Fe street

• Currently distributing to 2,500 Southern California locations

Brewing Process

Karl Strauss Products

Employment Opportunities• Master Brewer• Sales Representative• Customer Service• Chemist

• Quality Assurance

• Taste Testers• Distribution

If you have a good idea, you have to know how to write it well- Steve Kenney (eStudy Site)

“However long we live, our lives are but a brief moment in the trajectory from the past into the future. Nevertheless, it is long enough to make a difference, for better or for worse, in our lives and in the lives of others, both in our time and beyond.”– Jonas Salk [Mission : fund biomedical research

aimed at understanding diseases…]

SALK INSTITUTEhttp://www.salk.edu/

Salk Institutehttp://www.salk.edu/index.php

Sector:• Diagnostic Research Biotechnology• Medical Pharmaceutical Biotechnology• Agricultural BiotechnologyStage of Growth:• Research and Development• Collaborative / International• Master Plan Update 2006• Motto—Where Cures Begin…

Salk InstituteEmployment

Staff of ~1,200 from 50 countries (59 faculty, 300postdoctoral fellows & 100 graduate students) Current Openings:1. Faculty Positions-Senior Research/Investigator2. Postdoctoral Training-Bioinformatics, Research,

Biophysics, Gene Expression, Neuroscience…3. Staff Positions-Custodial Supervisor, Animal

Technician, Lab Assistant, Research Assistant, Micro-Injectionist, IT technician, Bioinformatics…

Salk InstituteTimeline

• 1952 – Dr. Jonas Salk discovers the polio vaccine• 1960 – Architect Louis Kahn designs the institute• 1962 – Groundbreaking of Institute (March of Dimes)• Ongoing –

Home to 11 Nobel Laureates – Drs. Frances Crick, Ronato Dulbecco, Roger Guillemin, Sidney BrennarOne of leading research institutes in the world…

• Areas of Research –Neuroscience-nervous system disorders Biology and Genetics-cancer & diabetes Infectious Diseases-AIDS & anthraxPlant Biology-food supply & global warming

Salk InstituteTechnology

Tools & Equipment: [shared w/other labs]• Light, Fluorescent, & Electron Microscopes• Computers Simulators, Flow Cytometer, etc.• DNA Synthesis, Sequencing, & Thermocyclers (PCR)

Core Technology:• Bioinformatics – comparative genomics & evolutionary• Cytometry & Molecular Imaging (CCMI) – sort cells, etc.• Mass Spectrometry/Proteomics – protein identification• Microarray/qPCR – gene expression profiling• Transgenic – transgenic & knock-out mice, IVF, embryo

cryopreservation & rederivation

Each member of a row team practices what seems a repetitive task, but it moves them toward a glorious goal. –Sandy Slivka (Miramar College)

Genentechhttp://www.gene.com

Genentech VisionUtilize the science of

biotechnology to become a leader in revolutionizing the

treatment of patients with cancer,

immunological diseases and

angiogenic disorders.

www.gene.com

Oceanside Facility

Manufacturing facility for Monoclonal Antibody-based pharmaceuticals; primarily Avastin®.– In Phase III trials– 1 month -> 18 kg -> $70,000,000.– “Smart Bomb” approach to angiogenesis

inhibition.

Working at Genentech

Employment Opportunities– Operations– Facilities– Manufacturing– QC/QA– Accounting– HR– Environmental Awareness (Sustainability)

Post-Secondary Education:– Educational reimbursement up to $10K/yr

Undergraduate Opportunities:– Summer Internship

programDesired Skills:

– Detail Oriented– Safety Oriented– Good Work Ethic– Technical Skills – Record Keeping– Flexibility

I used to make nail polish and lipstick. Although I made a great contribution to society, I find this more

rewarding- Mike Greig (Pfizer)

Who are they?• “The Home Depot of the Life Science Industry”- Lisa Peterson• Mature company that markets and manufactures a broad array of

tools for all sectors of the biotechnology industry• Founded in 1987, headquartered in Carlsbad, CA• Went Public in 1999 and acquired 18 different biotech companies

from 1999-2005• Operations in more than 20 countries and distribution in more than

60 countries• Revenue of $1.15 billion in 2006• For further information Invitrogen Web PageAudio and Video Profiles of Invitrogen

What is Their Core Technology?Tools For Complete Solutions

Products KitsType Title Here

Workflow systemsType Title Here

Technology

75% Drug Discovery Services and 25% Cell CulturesProducts- Pico GreenKits- PathAlertSystems- Qubit or QdotWorkflows- Protein Profiling

PathAlert™ Detection System

Qdot® nanocrystals

Employment Outlook• Over 4,300 employees

worldwide • 85 job openings in

Carlsbad, CA, for high school graduates to those with graduate degrees

• Some job openings:-scientist -business analyst-patent agent-IT director-accountant-production technician -technical writer-sales and marketing -project manager

You need to identify your passion and pursue it relentlessly .

“I like to work with my hands”- Al Spada (Conatus)

• Medical/Pharmaceutical Domain Company– pan caspase inhibitors

• Small molecule drug

• Startup Company– Split from Idun in 2005– Phase 2 clinical studies

(CTS-1027)– Develop a previously

identified potential drug

Timeline• 2/05: Pfizer acquires Idun• 7/05: Idun management team founded

Conatus• 8/06: New facility & personnel increase• 1-5/07: Secured 55 million in private

funding for phase II clinical studies• 11/07: File IND for phase II clinical

studies• 12/07: Begin clinical studies• 2008: License or collaborate on a

second Liver Disease Drug

• Pan caspase inhibitor Treatment for liver disease– inflammation – cellular degradation– Viral infection (Hepatitis C)

LSSI has broken down all the obstacles of bringing biotechnology to the classroom- Victor Rodriguez

(LSSI Teacher)

www.estudysite.com292 Euclid Avenue, Suite 225

San Diego, CA 92114

• Inpatient and outpatient medical research site providing phase II through III studies.

• Innovative business model– Utilize emergency department as a gateway for

inpatient and outpatient studies– Vast ethnic and geo-diverse database– Rapid enrollment and retention of a large number of

patients– Partnership with hospitals and physicians– Consistent “on-time” completion

Overview

Current Studies

• Medical– Infectious Diseases

• Pneumonia • MRSA

– Acute Coronary Syndrome– Hypertension

• Central Nervous System– ADHD– Insomnia– Schizophrenia

Types of Employment

• Physicians• Registered Nurses• Quality Assurance• Site Manager• Clinical Research

Coordinator• Regulatory Specialist• Pharmacist

I have had many good changes made by the PCR fairy- Brian Miller(Biogen idec)

DowpharmaA Business Unit of Dow

44 Biopharma R& D EmployeesComputer ScienceBioinformaticsChemical EngineeringMolecular BiologyMicrobiologyBiochemistry

5501 Oberlin DriveSan Diego California92121 U.S.A.Phone: 800-745-7475 Fax: 858-352-4560

56

Dow Biopharma Core Technologies

The next generation microbial expression system

Protein expression in P. fluorescens

VLP Vaccine CarrierAdvantaged performance for subunit vaccines

W ild-type VLP Modified VLP displaying a dense array of epitopes

Epitope

Epitope

CP surface loopAntigen W ild-type VLPW ild-type VLP Modified VLP displaying a dense array of epitopes

Epitope

Epitope

CP surface loopAntigen

Post - induction

Pre - induction

proc

ess a

nalyt

ical

bioinf

orma

tics

syste

ms bi

ology

DNA constructsDNA constructs

strain selectionstrain selection

fermentationfermentation

primary recovery primary recovery

purificationpurification

characterization and analysischaracterization and analysis

UPST

REAM

DOW

NSTR

EAM

MIDS

TREA

M

It is not what you know, it is how you do your job- Sandi Slivka (Miramar College)

Sector: Research, Testing and Medical Labs(1000 employees;100,000 worldwide)

Growth Stage: La Jolla – R & D

Including the Pfizer Incubator - a collaboration with Biotechnology startups and ScrippsJuly 18, 2007 Pfizer Announces First Occupant in its Science Incubator

Pfizer - Production and R & D Pfizer is the world's largest privately funded biomedical research organization.

Job Openings: Over 50 jobs ranging from lab technician to associate director positions.

La Jolla

History: In 1849, cousins Charles Pfizer and Charles Erhardt founded Charles Pfizer & Company in a red brick building in Brooklyn, NY.

Current: Pfizer's roster of drugs, like Lipitor, Celebrex, Viagra, Zoloft, and Zyrtex, grows with the launch of Sutent (developed at La Jolla) , a new, oral, cancer drug treating kidney and

intestinal cancer.

Pfizer Pipeline Portfolio

La Jolla

Tools & Core Technology

• High throughput chemistry and pharmacology, all types of mass spectrophotometers

• Monoclonal Antibodies• Structural and computational

biology• Cell Cultures • Molecular design• Molecular Biology

• Adaptable• Withstand disappointments

(9/10 drugs will fail)• Multidisciplinary/ Collaborative

Work force• Team Player• Work/Life Balance

Mass Spec and output

What kind of person??

Be Knowledgeable of future trends for your career path- Jennifer Cayer (Conatus)

Cells are the Labor

We at “Bio Gen Idec” are the supervisors

#1 Cancer Therapy World Wide

Non-Hodgkin’s Lymphoma

Rheumatoid Arthritis

Multiple Sclerosis- Relapsing Forms

Crohn’s Disease & Rheumatoid Arthritis

#1 MS Therapeutic Drug

Multiple Sclerosis- Relapsing Forms

Multiple Sclerosis- Monosymptomatic

1st In Class RIA Therapeutic

Non-Hodgkin’s B-Cell lymphoma

Currently Approved Drugs

Community Outreach• Biogen Idec Foundation &

Corporate Giving- science education and community service programs through grants.

• Externships- high school and college educators gain exposure to the life sciences industry, resources and teaching tools.

• Educational grants to medical schools, hospitals and community based healthcare-related organizations.

• Community Labs- research centers, dedicated to introduce middle and high-school students to hands on biotechnology, and encourage them to seek careers in the biomedical sciences.Since August 2002– 8776 students– 410 Teachers– 308 Employee Volunteers

Career Opportunities

• Biopharmaceutical Sciences (Manufacturing)

• Finance, Sales & Marketing

• Legal • Medical Affairs

• Medical Research • Research &

Preclinical Development

• Regulatory • Sales & Marketing

You are the unsung heros, the future scientists are in your hands- Octavio (Dow Pharma)