Leica Microsystems product catalog EN

Innovative Products and Solutions2007/2008

� Home �

A Powerful Vision!

That’s Leica MicrosystemsLeica Microsystems is a leading global designer and producer of innovative,high-tech, precision optical systems for the analysis of microstructures. It is oneof the market leaders in each of its business areas: Microscopy, Confocal LaserScanning Microscopy with corresponding Imaging Systems, Specimen Prepa-ration, and Medical Equipment. The company manufactures a broad range ofproducts for numerous applications requiring microscopic imaging, measure-ment, and analysis. It also offers system solutions for life science includingbiotechnology and medicine, research and development of raw materials, andindustrial quality assurance. The company is represented in over 100 countrieswith 7 manufacturing facilities in 5 countries, sales and service organizations in20 countries and an international network of dealers.

� Home �

• Light Microscopes– Education . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6– Bio/Med Routine Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10– Bio/Med Research Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17– Bio/Med Research Automated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19– Application Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21– Industry Routine Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27– Industry Research Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30– Industry Research Automated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33– Forensic Micro- and Macroscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

• Confocal Microscopes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

• Stereomicroscopes– Education . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44– Quality Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47– Routine Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50– Research Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57– Research Automated . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72– Macroscopes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75– Colposcope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

• Imaging Software– Cytogenetic Workstation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80– Materials Workstation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81– Fluorescence Workstation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83– Imaging Workstation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84– Imaging Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Contents

2 � Home �

• Camera Systems– Digital Photo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90– Analog Photo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

• Surgical Microscopes– Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104– Surgical Microscopes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107– Video Recording System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121

• Histology Systems and Materials Sectioning– Laboratory Printer Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124– Tissue Processors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125– Embedding Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127– Microtomes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129– Cryostats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142– Stainers, Coverslippers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150

• EM Sample Preparation– Ultramicrotomes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 160– EM-Laboratory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163

3� Home �

� Home �4

� Home �

Light MicroscopesLight Microscopeswww.leica-microsystems.com/Light_Microscopes

http://www.leica-microsystems.com/Light_Microscopes

� Home �

The expectations even for starter micro-scopes are growing all the time. With our150 years of experience and competence inmicroscopy, we play a major role in shapingdevelopments in this area. That’s why wedesigned the Leica BM E. Thanks to itscompact build, easy access controls, 45°viewing angle and 360° rotatable obser-vation tubes, users of all shapes and sizeswill be able to work at this microscope withease and convenience. The Leica BM E hasan extremely small footprint, is easy tocarry and stays cool in spite of its powerfulillumination. A blue filter is integrated toprevent loss. And the low position of the x/ystage minimizes wrist movement.

Leica BM ECompound microscope for education

6

Education

Range of use:� Education

� Home �

The Leica CM E has been specially designednot only to satisfy the growing demands forperformance in a first level university micro-scope but also to set new standards. It isunique in its design, user-friendliness andperformance. The compact size of the LeicaCM E keeps key controls within easy reachfor long spells of fatigue-free microscopyand takes up less table and storage space.Besides being easy to carry, it has 360°rotatable viewing bodies for comfortablesharing. You can count on the wide range ofaccessories and affordable price that youhave come to expect from Leica Micro-systems.

Leica CM ECompound microscope for education

7

Education


� Home �

The Leica DM E compound microscope isdesigned for general biology and specificlife science applications in university edu-cation and routine laboratory applications.Its highly efficient illumination system witha more powerful halogen lamp than anyother microscope in this class providesconsistent color and intensity throughoutthe whole lamp life (over 2000 hours). TheLeica DM E will enhance the quality of yourmicroscopy work. Equipped with the tech-nology of a research microscope, e.g.optional Koehler illumination and phasecontrast, polarization or darkfield illumina-tion, it opens up new horizons not only forone microscopist, but, using the multiviewingfacility, up to ten at a time.

Leica DM ECompound microscope for education

8

Education


� Home �

The Leica DM EP combines strain-freeoptics and precision-engineered polarizingmechanisms into an ergonomically advan-ced design that delivers accurate, high-contrast image quality for use in bothuniversity and laboratory settings. Leicaincorporated a range of exclusive featuresinto the DM EP, including a highly efficientKoehler illumination system that features a35 W halogen bulb, a voltage sensing powersupply that delivers optimized light intensityregardless of voltage fluctuations, anAnalyzer/Bertrand Lens module withswing-in/out controls, a rotating swing-in/out polarizer and a centerable condenser.The microscope can easily be enhancedwith a wide range of accessories that allowenhanced polarized microscopy applica-tions and educational and analytical photo-micrography.

Leica DM EPPolarization microscope for earth, material and forensic science

9

Education

Range of use:� Education� Industrials & Materials

� Home �

The Leica DM IL is the inverted contrastingmicroscope of choice for microbiology andcell culture laboratories. Offering virtuallyunlimited application potential in live cellmicroscopy, it is ideal for routine examina-tions of cell and tissue cultures, for liquidsand sediments and for special applicationssuch as micromanipulation and microin-jection. Users will be impressed by itsbrilliant incident light fluorescence, opti-mized phase contrast, and a new, extremelyefficient contrasting technique:For the first time, Leica’s unique IMC(Integrated Modulation Contrast) enableshigh quality Hoffman modulation contrastwithout having to use special objectives.

Leica DM ILInverted contrasting microscope for living cell applications

Bio/Med RoutineManual

10

Range of use:� Clinical� Life Science Research

� Home �

All variants of the DM1000–3000 series arespecially designed for use in clinical labor-atories, although they are equally suitablefor any other biomedical application fromroutine to research. Thanks to the ergonomicdesign, users can work at the microscopefor long periods without suffering fromneck, shoulder and back muscle tension.The HI PLAN SL (synchronized light) objec-tives encourage hours of fatigue-freeviewing. All variants feature unique height-adjustable focus controls and can be quickly changed from right- to left-handoperation. They satisfy all requirements ofoptical brilliance, and are particularly suitable for cytology, pathology, and hae-matology. As system microscopes LeicaDM1000 and DM2000 are also convenientfor basic research microscopy applicationsincluding fluorescence.

Leica DM1000 & DM2000System microscope for universities, medical practices and routine applications


11

Range of use:� Education� Clinical� Life Science Research

� Home �

The Leica DM1000 LED is equipped withlong-life LED illumination, which featuresthe following advantages: akin to daylightand bright illumination, less heat emission,as well as no need for lamp changes. Leicaalso offers a portable, solar-powered option for field use. Battery operation isalso possible and allows flexible utilizationat different places of work. Users of theLeica DM1000 LED benefit from the sameergonomic, performance-enhancing advan-tages as the Leica DM1000. It is ideal for allclinical laboratory applications, especiallyfor cytology, pathology, and haematology.

Leica DM1000 LEDSystem microscope with LED illumination for universities, medical practices and routine applications


12


� Home �

Leica DM2500 with its powerful 100 Willumination and its optimized optical per-formance is especially suited for morecomplex tasks in pathology or biomedicalresearch that require interference contrastor high performance fluorescence. Thanksto its application-oriented design it can beconfigured to fit demanding challenges andit gives the customer a research stand witha 100 watt light source. This brightillumination is helpful particularly forDifferential Interference Contrast work. TheLeica DM2500 can be configured to fitphysical requirements of the customerusing a variety of observation tubes, ergo-nomic modules, and integrated adjustablecontrols.

Leica DM2500System microscope for universities, medical practices and routine applications


13


� Home �

With its intelligent and speedy automation,such as unique toggle mode, motorizednosepiece, condenser, automated lightintensity adjustment, and optional footpedal, the Leica DM3000 supports greaterefficiency and enhanced user comfort. Aswith the other variants of the DM1000–3000series, replacement of the halogen lamp iseasy and convenient due to the specialdrawer. The automated objective turretchanges objectives in only half a secondand objectives can be individually selectedusing the control buttons ergonomicallypositioned behind the focus knobs. Thisintuitive microscope improves the workflowin cytology and pathology laboratories aswell as in all other biomedical routine andresearch environments.

Leica DM3000System microscope for universities, medical practices and routine applications


14


� Home �

Excellent Imaging with a New Degree ofFreedom!

Leica Microsystems has designed a trueinnovation: a new network imaging solutionthat addresses the growing workload intoday’s busy pathology laboratory with fast,easy digital microscopy. The Leica DMD108Digital Microimaging Device reduces physicaldiscomfort and can speed pathologists’daily workflow with a modern digital micro-scope network solution for sharing data. For the first time ever: You can see high-resolution images that differentiate betweenthe slightest nuances of color directly on amonitor without having to look throughmicroscope eyepieces.

Leica DMD108Digital microimaging device for clinical diagnostics labs


15

Range of use:� Clinical

� Home �

The Leica DMI3000 B with manual standwas specially designed for customers whowork without fluorescence. Offering alltransmitted light contrast techniques incl.POL, DIC, and the unique Integrated Modu-lation Contrast (IMC) the inverted microscopeis suitable for life science research androutine examinations such as scanning ofcell and tissue cultures. The LeicaDMI3000 B outperforms not only the latesttechnical standards but also all ergonomicexpectations – and you can combine it withany of the solutions from Leica's productrange.

Leica DMI3000 BInverted microscope for biomedical research

16



� Home �

The upright Leica DM DigitalMicroscopeseries offers an intelligent automation con-cept for demanding applications in routineand research. Contrasting techniques canbe switched at the press of a button –microscopy has never been so easy. AnIllumination Manager ensures optimal lightintensity and diaphragm settings for allcontrasting methods. All settings can natu-rally be matched to your specific require-ments. Besides being easy to use, themicroscopes feature brilliant optics, anergonomic design, and an impressive rangeof accessories. Leica DM4000 B, DM5000 B,and DM5500 B – a unique combination ofoperational convenience and state of-the-art technology.

Leica DM4000 B, DM5000 B & DM5500 BResearch microscope system for life science

17

Bio/Med ResearchManual


� Home �

The inverted Leica DMI4000 B was designedfor applications in life science. Outperform-ing not only the latest technical standardsbut also all the ergonomic demands of amodern microscope, the automated researchmicroscope is also suitable for scanningcell and tissue cultures.With its completely new fluorescence axisit guarantees ultra-brilliant fluorescence.The internal filter wheel with motorizedExMan and FIM enables excitation offluorochromes in less than 20 milliseconds.The Leica DMI4000 B can be combined withany of the solutions from Leica's productrange.

Leica DMI4000 BAutomated inverted microscope for biomedical research

Bio/Med ResearchManual

18


� Home �

Be inspired by the fully motorized micro-scopy system with intelligent automationconcept. As digital microscope platform theupright Leica DM6000 B allocates all trans-mitted light contrasting methods includingthe world’s first fully automated differentialinterference contrast (DIC). With absolutereproducible shearing and bias values.Motorized z drive and nosepiece provideunrivalled ease of use and convenience.Dedicated software solutions in combina-tion with the motorized fluorescence axismake the Leica DM6000 B a powerfulresearch microscope for applications likeimmunofluorescence with deconvolutionoption, and live cell imaging.

Leica DM6000 BResearch microscope system for life science

19

Bio/Med ResearchAutomated


� Home �

Visibly more – more visibility: new differen-tial interference contrast (DIC) for reliefimaging of specimens with varying indicesof refraction. Contrast and illuminationmanager. Motorized Z focus and parfocalityfunction. Automatic brightness and dia-phragm adjustment. Automated fluores-cence axis with fluorescence intensitymanager, excitation manager, internal fastfilterwheel and two integrated shutterswith remote control support. Synchronousmultiple color visualization of cell compart-ments. Separation of GFP variants andfluorescence stains in less than 0.05 sec.Motorized fluorescence balancing ofspecimens with multiple stains. Leica lighttrap for brilliant fluorescence.And as always with Leica, intuitive operation.

Leica DMI6000 BFully automated inverted research microscope for biomedical research

Bio/Med ResearchAutomated

20


� Home �

The Leica AF6000 is a fully integratedsystem for advanced fluorescence imaging,providing solutions that evolve with yourchanging research requirements. Fromoverlaying multi-channel images to acquir-ing three dimensional and time lapse data,a wealth of solutions are included asstandard for image documentation, quantifi-cation, enhancement and analysis. Designedto completely harmonize microscope, cam-era and application, the modular AF6000system is available for both upright andinverted microscopes. Additional applica-tion modules can be added to extend thefunctionality for deconvolution and 3Dvisualization.

Leica AF6000Advanced fluorescence imaging system

21

Application System

Range of use:� Life Science Research� Fluorescence Workstation� Fluorescence Imaging

� Home �

The Leica AF6000 LX is an integrated systemfor advanced widefield life cell imaging andanalysis. This ultra-fast system offers theultimate in hardware and software integra-tion to study the processes of life. Imagingfast cell dynamics or 4D experiments overseveral days can easily be performed.Carefully selected components ensure the necessary stability for long term experiments, keeping the cells in optimalcondition.

Leica AF6000 LXAdvanced fluorescence imaging and live cell analysis system

Application System

22

Range of use:� Life Science Research

� Home �

The first application solution to combine thefunctions of a fully automated inverted re-search microscope with those of electronicmicromanipulators.Both systems can be operated via the LeicaAM6000 from a clearly designed joint controlbox. We have integrated all the main controlsof the two basic instruments (microscopeand micromanipulator) into one central unitand matched the functions to each other.The result: improved operating safety, redu-ced vibrations within the system and aconsiderable time saving, both for workroutines and for staff training. With the Leica AM6000 there’s no need tokeep switching between controls in future.You have everything in one hand. Move-ments of the micromanipulators arecorrelated to the magnification of themicroscope, electronically.

Leica AM6000System for production of transgenic animals

23

Application System

Range of use:� Life Science Research� Micromanipulation� Telepathology

� Home �

The new MultiColor TIRF (Total InternalReflection Fluorescence) from LeicaMicrosystems is an all-in-one systemoffering four integrated solid state lasersfor excitation of fluorophores in all importantwavelengths. The extremely short switchingtimes, the automatically constant TIRFpenetration depth when switching from onewavelength to another and the extremelyhigh and synchronized image recordingrate open up completely new horizons forresearching dynamic processes in livingcells.

Leica AM TIRF MCTIRF (Total Internal Reflection Fluorescence) microscope system with multi laserbox

24

Application System

Range of use:� Life Science Research� Total Internal Reflection Fluorescence� Fluorescence Workstation� Fluorescence Imaging

� Home �

Contamination-free, fully automated lasermicrodissection system for targeted cellisolation simply using gravity and a UV diode laser.Single cells or groups of cells can be micro-dissected from tissue sections, biopsies,smears, cytospins, and cell cultures. Thelaser can be also applied for intracellularand cellular ablation. Nucleic acids andproteins specifically isolated from thedissected specimens can be directed tomolecular analyses such as: sequencing,genotyping, PCR, real-time PCR, 2D gelelectrophoresis or MALDI.The laser microdissection system is basedon the fully automated upright researchmicroscope Leica DM6000 B, the idealcombination for laser microdissection.

Leica LMD6000Laser microdissection system

Application System

25

Range of use:� Laser Microdissection

� Home �

With its ultrahigh frame rate and gentlehandling of samples, Leica’s SD6000 Spin-ning Disk Confocal Unit is ideal for live cellexperiments. Used together with the LeicaAF6000 LX Live Cell Station, the advantagesof high-resolution confocal microscopy arecombined with those of widefield fluores-cence in one single system. One simplekeystroke switches between confocalimage recording, widefield fluorescence, a transmitted light method or even the optional TIRF technique. One and thesame highly sensitive fluorescence cameraserves as the detector for all these methods.

Leica SD6000Spinning Disk Confocal option for Leica AF6000 LX

26

Application System

Range of use:� Life Science Research� Fluorescence Workstation� Fluorescence Imaging

� Home �

The Leica DM ILM inverted microscopewas specially designed for all inspectionand measurement tasks in metallographyand industrial materials testing. High-perfor-mance Leica HCS optics (Harmonic Compo-nent System) guarantee optimal conditions:maximum image resolution and perfectimage contrast in incident light brightfield,polarization contrast plus fluorescence.The Leica DM ILM scores ergonomy-wise,too: all inspection and measurement taskscan be performed quickly and efficiently.

Leica DM ILMInverted routine microscope for material testing

27

Industry RoutineManual

Range of use:� Industrials & Materials

� Home �

The Leica DM2500 M allows you to improveyour workflow and concentrate entirely onthe task at hand. Microscope operationbecomes secondary to your investigations.Viewing your material examinations in thebest light is our top priority. Equally impor-tant is our commitment to always offer youthe best quality optics and most efficientmicroscope systems in the business. Youneed a materials microscope system de-signed for rapid, accurate results. Leicamicroscope systems are designed to de-crease your bench time and provideoptimized results. The Leica DM2500 M willshow you how simple and reliable micro-scopy can be.

Leica DM2500 MSystem microscope for material testing

28



� Home �

The Leica DM2500 P is designed for all routi-ne polarizing examinations in: petrography,mineralogy, structure characterization,examination of liquid crystals and fibers.With versatile instrument options, the LeicaDM2500 P polarizing microscope is also anideal match for industrial analysis andquality control, such as analyzing glass,plastics, textiles and fibers or testing dis-plays in the semiconductor industry.

Leica DM2500 PPolarization microscope for transmitted and reflected light

29



� Home �

The Leica DM4000 M is equipped with anincident-light axis and can be used with allcommon incident-light methods (all of themautomated upon request). The axis featuresa 4x reflector disc with 2 fixed mounted and2 changeover positions for instrumentationwith reflectors or fluorescence filter cubes.A transmitted-light axis works with allknown transmitted-light methods (bright-field, darkfield, phase contrast, polarizationcontrast, interference contrast – all auto-mated). As expected from a routine micro-scope, the Leica DM4000 M operates with amechanical Z-drive; and the stage is alsooperated mechanically. The 6x objectiveturret with M32 thread is absolute coded sothat the objective used is immediatelydetected. All current setting values can becalled up at a glance using the clearlydesigned status display.

Leica DM4000 MResearch microscope system for material and geo science

30

Industry ResearchManual


� Home �

Leica DM4500 P for research and develop-ment is designed for all polarization exami-nations in: petrography, mineralogy, structurecharacterization, examination of liquidcrystals and fibers.With versatile instrument options, the LeicaDM4500 P polarizing microscope is also anideal match for industrial analysis andquality control, such as analyzing glass,plastics, textiles and fibers or testingdisplays in the semiconductor industry.

Leica DM4500 PPolarization research microscope system for material, geo and life science

31



� Home �


Our mission is to visualize your materialsresearch in the very best light. Our optical& design engineers focused their entireexpertise on this goal. The result is theLeica DMI5000 M, the successor of thefamous Leica MeF4. However, it is not onlythe best possible image quality that drivesus. The intelligent operation of the LeicaDMI5000 M will let you experience thepleasure of professional microscopy with-out the work.Using a microscope has never been thissimple; the DMI5000 M can always be configured to suit your application require-ments. It is the optimum solution for your tasks in R&D, quality assurance andtesting.

Leica DMI5000 MInverted research microscope for material testing

32


� Home �

The current state of the art of our DMDigital Microscope family. As a high-endautomated research microscope, the LeicaDM6000 M leaves nothing to be desired,and leaves no question unanswered. Theautomation of this microscope, everymodule of which is motorized, is brilliant.Together with our new digital cameras,which have been specially tuned to matchthe DM Digital Microscope series, as wellas software products for image analysisand image archiving, you will receive asystem that is custom-tailored for yourwork.

Leica DM6000 MResearch microscope system for material and geo science

33

Industry ResearchAutomated


� Home �

The new Leica FS C motorized ComparisonMacroscope is a modular system for allcomparison applications in the forensiclaboratory, whether for comparing marks on fired ammunition parts, toolmarks or documents.It combines an innovative, intelligent auto-mation concept with outstanding ergonomicfeatures. Its completely new optics com-prise five apochromatically corrected macroobjectives with a high numerical apertureand adjustable iris diaphragms for top opti-cal performance.The enhanced reproducibility and accuracyoffered by this state-of-the-art platform willimprove the efficiency of forensic examina-tions. Different imaging modes (split image,superimposed image and combined image)can be set at the press of a button.

Leica FS CComparison macroscope for forensic examinations

34

Forensic Micro- &Macroscope

Range of use:� Forensic

� Home �

The Leica FS CB features two brand newlab-class microscopes, the Leica DM2500,under the motorized comparison bridge.This ergonomic work scenario is ideallysuited for all microscopic applications thatrequire a direct side by side comparison.The Leica FS CB enables high precisioncomparison of two objects at magnifica-tions up to 1500x, supplying reliableevidence of the tiniest differences in theirmicrostructure, texture and colour. All theusual contrasting techniques such asbrightfield, fluorescence and polarizationare provided and can be easily applied. TheLeica FS CB features a motorized com-parison bridge with built-in ergonomicobservation tube for maximum viewingcomfort. Various imaging modes (splitimage, superimposed image and combinedimage) can be set at the press of a button.

Leica FS CBComparison microscope system for hair & fiber analysis in the forensic lab

35



� Home �

The Leica FS4000 combines the latestoptomechanical knowledge in the field oflight microscopy with unequalled userfriendliness and ergonomy in an integratedsolution, allowing the user to concentratetotally on his work and achieve highlyprecise and efficient results.The Leica FS4000 enables high precisioncomparison of two objects at magnifi-cations up to 1500x, supplying reliableevidence of the tiniest differences in theirmicrostructure, texture and color. All theusual contrasting techniques such asbrightfield, fluorescence and polarizationare provided and can be selected in a frac-tion of a second. Various imaging modes(split image, superimposed image andcombined image) can be set at the press ofa button.

Leica FS4000Comparison microscope system for trace evidence

36



� Home �

Confocal MicroscopesConfocal Microscopeswww.leica-microsystems.com/Confocal_Microscopes

http://www.leica-microsystems.com/Confocal_Microscopes

� Home �

As the only broadband confocal, the LeicaTCS SP5 fully covers the main requirementsin confocal and multiphoton imaging withoptimal overall performance and providesthe full range of scan speedsat highest resolution.

With its well proven high efficiency SP-detection (five channels simultaneously)and optional AOBS for dynamic beam split-ting, the Leica TCS SP5 delivers bright,noise-free images with minimal photo-damage at high speed.

The system is the platform for the new fixedstage microscope DM6000 CFS for physio-logical and electrophysiological experimentsand for the new superresolution fluorescencemicroscope Leica TCS STED.

Leica TCS SP5High speed and high resolution spectral confocal

38

Confocal Microscopes

Range of use:� Confocal Microscopy� Multiphoton Microscopy

� Home � 39

The Leica TCS SPE is the new high resolutionspectral confocal for daily research androutine examination. The highly integratedsystem is optimized for target applicationsin research laboratories and pharma-biotechnology. Providing all importantfeatures, it offers spectacular imaging at anattractive price.

The newest technology is implemented inthe Leica TCS SPE and this system is theonly confocal in its class providing truespectral detection.

The TCS SPE is very easy to use and firstresults are quickly achieved, even by con-focal newcomers. Its robust, durable hard-ware with long-life components and thenew Leica software platform LAS AF ensuresmooth and fast operation.

Leica TCS SPEHigh resolution spectral confocal


Range of use:� Confocal Microscopy

� Home �

Enter the fluorescence nanoworld bysuperresolution with the Leica TCS 4PI, thefirst commercial 4Pi system worldwide.

With its spectacular resolution enhance-ments and 3D capabilities it allows preciselocalization of cell constituents, parasitesand viruses in three dimensional cell space.Structures far too small for the resolution limits of widefield and confocal micro-scopes can now be investigated with theLeica TCS 4PI.

This microscopy system is the answer tothe contemporary need in the biomedicalenvironment for higher spatial resolutionwhile maintaining capabilities of live cellimaging and keeping existing protocols forstructure specific fluorescence labeling.

Leica TCS 4PIHigh-resolution 4Pi fluorescence system

40


Range of use:� Superresolution Light Microscopy

� Home � 41

The Leica FCM1000 is the first imagingsolution developed for – and fully adaptedto – in vivo and in situ small animal imaging.

In vivo observation of live processes requi-res a high degree of miniaturization forminimally invasive access as well as anultra-high frame rate for real-time dynamicrecording.

With its fibered microprobes the LeicaFCM1000 is designed to access virtuallyanywhere in the living animal. A simplecontact with the tissue of interest is enoughto generate high-speed recordings ofcellular or vascular events.

Leica FCM1000Fiber Confocal Microscopes


Range of use:� Endoscopic Confocal Microscopy

� Home �42

� Home �

StereomicroscopesStereomicroscopeswww.leica-microsystems.com/Stereomicroscopes

http://www.leica-microsystems.com/Stereomicroscopes

� Home �

The Leica ES2 is the perfect solution for thehigh-school market. From the 2-magnifi-cation starter model to the digital zoommodel with its integrated 3-megapixelCMOS camera, the new Leica educationalstereomicroscopes combine superb opticaland illumination quality with ease of useand comfort for extended use. The ruggeddesign is completely maintenance-free andis built for rough-and-tumble school environ-ments. Like all our instruments, the quality,lead-free optics and recyclable housingsatisfy environmental management require-ments.

Leica ES2Educational stereomicroscope, 2-step, 10x/30x, integrated LED illumination

44

Education


� Home �

The new Leica E-series stands out from thecrowd of educational stereomicroscopes byoffering the best value for money and thefollowing features: Leica’s typical high imagequality, color and detail fidelity, mechanicalprecision for decades of maintenance-freefunctionality, precise zoom and focusingsystems for the most exact control, powerLED illumination system for incident andtransmitted light. Our unique Leica 3-wayincident light technology on all Leica EZ4models provides observers of a wide varietyof objects – from strongly structured objectsto flat probes – with optimal illumination toobtain a maximum of information. The inte-grated LEDs can be switched individually,dimmed and combined with transmit ted light.The membrane switch that controls theilluminator is inte grated in the base and has awatertight seal.

Leica EZ4Educational stereomicroscope, 4.4:1, 8x-35x, integrated LED illumination

45

Education


� Home �

The Leica EZ4 D, with its integrated 3-megapixel CMOS camera and Leica appli-cation software, allows the direct storageof image data on 128 MB SD card or theconnection to PC, Mac, video recorder orbeamer. The application software includedwith the Leica EZ4 D controls image captureand storage, live image display on connect-ed PCs or Macs, and the archival andopti mization of image data.

Leica EZ4 DEducational stereomicroscope, 4.4:1, 8x-35x, integrated LED illumination, integrated 3.0 MPixel CMOS camera

46

Education


� Home �

The Leica EZ5 stereomicroscope is a funda-mental component of any manufacturingsystem that requires precise optical testingduring equipment and component assembly,processing, and testing. If your manufacturingfacility requires accurate and reliable opticalinspection and testing, the Leica EZ5 is theideal solution.

The compact, lightweight Leica EZ5 stereo-microscope features high-performance opticsand provides crisp, sharp image quality com-bined with straightforward handling. And, thiseasy-to-use and cost-effective stereomicro-scope offers a multitude of features to ensurecomplete user comfort during operation.

Leica EZ5Stereomicroscope with 5:1 zoom ratio, 10x-50x magnification,100 mm standard working distance

47

Quality Control

Range of use:� Quality Control� OEM

� Home �

The Leica S4 E with 4.8:1 zoom and standardmagnification of 6.3x-30x is the basic model ofthe Leica StereoZoom® line.This complete line of stereomicroscopes withGreenough optical system offers an affordableand comprehensive program for all applica-tions, from manufacturing quality inspectionand assembly, OEM integration, and studentlaboratory use, to exacting research anddevelopment tasks.

Leica S4 EGreenough stereomicroscope

48

Quality Control

Range of use:� Quality Control� Routine Manual

� Home �

The Leica S6 T with 6.3:1 zoom and a stan-dard magnification of 6.3x-40x is the world’sfirst and only fully electrostatic dissipativestereomicroscope. This patented memberof the Leica StereoZoom line is essential forcritical inspection of ESD sensitive elec-tronic components such as computer harddrives.The Leica S6 T with incident light or T swivelarm stand is the ultimate in ESD protection.

Leica S6 TGreenough stereomicroscope

49

Quality Control

Range of use:� Quality Control� Routine Manual

� Home �

The Leica MS5 with five-step magnificationchanger is the most flexible routine stereo-microscope in the world. The Leica MS5 isin line with the Leica tradition of providingever-better facilities for ergonomic, fatigue-free working.

Leica MS5High-performance stereomicroscope with 5 steps

50

Routine Manual

Range of use:� Routine Manual

� Home �

The Leica MZ6 modular stereomicroscopewith 6.3:1 zoom covers important magnifi-cation ranges between 6.3x and 40x for thenon-destructive 3-D observation of unpre-pared objects in science, and for inspectionand assembly in technology.

Leica MZ6High-performance stereomicroscope with zoom 6:1

51

Routine Manual


� Home �

The Leica S6 D with 6.3:1 zoom and astandard magnification of 6.3x-40x is thedocumentation model of the Leica Stereo-Zoom line. This StereoZoom® with inte-grated photo/video port offers an afford-able and comprehensive documentationsolution for all fields of application likemanufacturing, quality inspection, smallparts assembly, OEM integration, studentlaboratory use or other research anddevelopment tasks.

Leica S6 DGreenough stereomicroscope

52

Routine Manual


� Home �

The S6 E with 6.3:1 zoom and a standardmagnification of 6.3x-40x has a comfortable38° viewing angle. The optical design of theS6 E, coupled with this ergonomic viewingposition allows this microscope to be usedfor long periods without causing eye strain,resulting in faster, more efficient inspec-tions, and increased productivity. Clear,sharp images are always produced by theLeica S6 E, as it is a chromatically optimizedand robustly manufactured microscope.

Leica S6 EGreenough stereomicroscope

53

Routine Manual


� Home �

The Leica S8 APO is the flagship of ourLeica StereoZoom® line. The instrumentoffers an 8:1 zoom with a standard magni-fication of 10x-80x. It is the world’s only highperformance Greenough style stereomicro-scope with a fully apochromatic corrected1.0x objective and zoom lens system. Re-searchers engaged in critical applicationssuch as microinjection or fine pitch wirebonding will find that our S8 APO makes aworld of difference in their work.

Leica S8 APOGreenough stereomicroscope

54

Routine Manual

Range of use:� Routine Manual� Research Manual

� Home �

Made for the Leica S4E and all S6 models,the high quality Leica LED2000 illuminationsuits all incident light applications toprovide a cost effective solution where notransmitted light is needed. With five illumi-nation options available, you can chooseexactly where and how much incident lightyou want to apply to your sample.

Leica LED2000Integrated incident light LED stand

55

Routine Manual


� Home �

Made for the Leica S4E and all S6 models,the Leica LED2500 includes both incidentand transmitted light capabilities, whichcan either be used together or controlledindividually. With a 60 mm active lightdiameter, the LED2500 is perfect for per-forming detailed inspection with consistentlight levels across samples with a largefield of view.

Leica LED2500Integrated incident and transmitted light LED stand

56

Routine Manual


� Home �

The Leica FluoCombi III is an extremelyuseful accessory for the Leica MZ16 F andMZ16 FA fluorescence stereomicroscopes.Expressed Drosophila, C. elegans, zebrafishor Arabidopsis can be sorted in the gene-rous three-dimensional field of view andscreened with the same instrument at 460xmagnification and 1500 Lp/mm resolution,e.g. in gene technology, in-vivo investi-gation or similar. In many cases, sensitivesamples or specimens no longer need to betransferred to a light microscope.Highest resolution up to the edges of thefield of vision: planapochromatic 5x highresolution micro objective.

Leica FluoCombi III™

Fluorescence from 3D to high resolution

57

Research Manual

Range of use:� Fluorescence

� Home �

Optimal illumination improves people’soptical performance and reduces errorrates. The use of cold light sources hasbeen catching on in stereomicroscopybecause they illuminate objects intensely.The Leica L2 cold light source is powerful,small, compact, affordable, and is suited toall applications in industry and science.Besides classically angled lighting with oneor two armed light guides, other equipmentis available for coaxial, vertical, and trans-mitted illumination methods.

Leica L2Cold light source

58

Research Manual

Range of use:� Illumination

� Home �

Efficient cold light fluorescence systemOn a worldwide level, the unique Leica MZFLIII fluorescence stereomicroscope hasbecome indispensable for qualified re-search and analysis tasks in genetics andmolecular biology. An easy-to-use fluores-cence system is now available for dailyroutine work, such as dissecting, selecting,and sorting exprimed models, as well as forforensics and industrial stereofluores-cence applications.The new Leica L5 FL fluorescence systemfor blue or green fluorescence can easilybe adapted, even subsequently, toLeica S and M stereomicroscopes to beimmediately operational. This provides theuser with an effective, handy, and easy-to-use instrument.

Leica L5 FLFluorescence system for blue or green fluorescence, adaptable toLeica S and M stereomicroscopes

Research Manual

59


� Home �

Leica MacroFluo introduces the world offluorescence to macroscopy and viceversa and lets you experience a newdimension of brilliant images of the highestpossible precision. For this purpose, wecombined the excellent optics of ourapochromatic Leica Z6 APO (6.3:1) andZ16 APO (16:1) zoom systems with brilliantLeica fluorescence technology.The result: the first and only macro docu-mentation systems for fluorescence andimages of a unique sharpness, precisionand depth of information.The unique feature of the MacroFluoconcept is the combination of large workingdistances and fields of view of a stereo-microscope with the vertical optical pathtypical of microscopes.

Leica MacroFluo™

High resolution, multidimensional fluorescence zoom system

60

Research Manual

Range of use:� Research Manual� Fluorescence

� Home �

The Leica MZ75 high-performance stereo-microscope with 7.9:1 zoom offers leading-edge optical technology at an affordableprice. Companies and users throughout theworld have the opportunity to experiencethe performance and the quality of a Leica.

Leica MZ7.5High-performance stereomicroscope with 7.9:1 zoom

61

Research Manual

Range of use:� Research Manual

� Home �

The Leica MZ95 high-performance stereo-microscope features an advantageous 9.5:1zoom ratio and magnifications up to 480x.The high resolution up to 300 line pairs permillimeter, extremely high image contrast,and amazing sharpness offer the ultimate inimage fidelity and data transfer for criticalinspection applications.

Leica MZ9.5High-performance stereomicroscope with zoom 9.5:1

62

Research Manual


� Home �

The new Leica MZ10 F fluorescence stereo-microscope offers splendid advantages forbiology, medicine, molecular biology andtechnology. Research into the functionsand interactions of living organisms re-quires fluorescence labelling, a procedurepermitting in-vivo observation of growthprocesses to gain an insight into thedistribution and development of certainstructures of living cells and tissues. Stereo-microscopes are used early in the researchprocess to screen samples and sort ordissect them. Offering a magnificationrange from 8x-80x and a high resolution of375 Lp/mm in the standard configuration,the Leica MZ10 F is the perfect choice fordaily routine tasks.

Leica MZ10 FHigh-performance stereomicroscope with 10:1 zoom

63

Research Manual


� Home �

The Leica MZ12.5 high-performance stereo-microscope with 12.5:1 zoom provides theuser with more information, more details,and with more knowledge. The perform-ance in terms of contrast, richness of detail,resolution, and color fidelity is unsurpassedand extends the limits of microscopic ob-servation.

Leica MZ12.5High-performance stereomicroscope with 12.5:1 zoom

64

Research Manual


� Home �

With the introduction of MZ16 and MZ16 A,Leica Microsystems celebrates a milestone inthe world of stereomicroscopy; these twoproducts are targeted at the high-end lifescience research and industrial inspectionmarkets that demand higher performance.With these two instruments comes the firststereomicroscope that provides a completelymotorized workstation, setting standards forour competition in automation and workingergonomy, and once again proving that LeicaMicrosystems is an innovator strictly focusedon meeting customers' needs.

Leica MZ16High-performance stereomicroscope with 16:1 zoom

65

Research Manual


� Home �

Worldwide, the manual Leica MZ16 F is themost powerful fluorescence stereomicro-scope. Due to its unique features withrespect to optical performance andfluorescence quality, the new Leica MZ16 Fis the ideal research and lab instrument fordemanding fluorescence microscopy exami-nations in biology, medicine, chemistry,electronics,geology, archaeology, aeronau-tics, cosmetics, pharmaceutical industry,agronomy, criminology, and much more.These are the most important arguments infavor of the Leica MZ16 F.

Leica MZ16 FThe world's first fully apochromatic fluorescence stereomicroscope

66

Research Manual

Range of use:� Research Manual� Fluorescence

� Home �

In the electronics industry, stereomicro-scopes are required for visual inspection oflarge printed circuit boards. The surfacesof cylindrical bearings in motors are visuallyinspected for quality during the automotiveproduction process. Biologists conductsurgeries on mice and rat brains to re-search how nerves conduct signals andregenerate after trauma. These are just afew examples of how large specimens, orthe tools required to test or manipulatethese specimens, require a special type ofstereomicroscope stand.

Leica Microsystems offers the perfectsolution: a new swingarm stand series. Thewide extension range of the swingarm,heavy load capacity, ability to connect afocus arm with a wide variety of adaptationoptions, outstanding vibration-dissipation –these are just a few of the many convenientfeatures.

Leica Swingarm StandsAccessory

67

Research Manual

Range of use:� Research Manual� Quality Control

� Home �

The Leica TL BFDF base features conti-nuously adjustable changeover betweenbrightfield and darkfield, providing highcontrast for stained amplitude specimens.It also offers versatile options. The lightrays can be deflected through the objectfrom steep to flat and a high or low degreeof diffusion can be selected. For example, if the light rays are deflected absolutelyvertically through the object, an exactbrightfield with maximum brightness is created. The specimen appears with fullcontrast and in natural color on a bright,homogenous background.

Leica TL BFDFTransmitted light stand for brightfield and darkfield applications

68

Research Manual

Range of use:� Research Manual� Research Automated

� Home �

Contrasting method for clear, transparentspecimens

The Rottermann ContrastTM technique is apartial illumination technique that showsthe changes of the refractive index asdifferences in brightness. Phase structuresthen typically appear as spatial, relief-typeimages – like hills in the positive relief-contrast and as indentations in invertedrelief contrast.

Together with the new Leica cold lightsource CLS150 LS, the TL RCTM enables theinternal shutter to be controlled directly viathe connected PC.

Leica TL RCTransmitted light stand with partial illumination technique for every brightness

69

Research Manual


� Home �

The transmitted-light base TL RCITM has twoUSB ports and two CAN bus interfaces. Thebrightness of the light source can be con-trolled via a USB mouse, for example. Incombination with the motorized Leicastereomicroscopes and macroscopes,motorized focus and the Leica ApplicationSuite (LAS) software, you are able to exer-cise full control via the computer over thezoom level, focusing, color temperature,brightness and shutter of the illumination. Alarge number of test series can thus beextracted from the Leica Application Suite(LAS) software and automated!

Leica TL RCI™

Transmitted light stand with partial illumination technique with USB ports

70

Research Manual


� Home �

Operating a transmitted-light base can beso simple: you have a potentiometer avail-able for setting the brightness. The mirrorguiding the light through the specimen atdifferent angles can glide across the hori-zontal plane. The tilt angle of the mirror isthen automatically aligned.

In this way you have full control of thetransmitted light without having to spend agreat deal of time looking for the optimumtransmitted-light angle.

The novel halogen lamp deserves a closerlook and is particularly efficient – with acapacity of only 20 W it reaches the lightutilization of the normal 35 W lamps. Theheating effect is noticeably reduced, whichis a particular benefit for temperature-sensitive samples.

Leica TL STFull control of the transmitted light in every angle

71

Research Manual


� Home �

The Leica thermocontrol system MATS(Microscope-stage Automatic Thermocon-trol System) is a heating system for micro-scopes and stereomicroscopes and allowsthe viewing of sensitive microscopicspecimens under accurate temperatureconditions.

The Leica MATS plastic stage frameconducts heat at a lower rate than metalallowing less heat to leave the stage areaand a more accurate thermal distribution tobe achieved.

Temperatures varying from room tempera-ture to 50°C are possible with this systemallowing every heating need to be captured.

Leica MA TSThermocontrol heating stages

72

Research Automated


� Home �

With the introduction of MZ16 and MZ16 A,Leica Microsystems celebrates a newmilestone in the world of stereo-microscopy; these two new products aretargeted at the high-end life scienceresearch and industrial inspection marketsthat demand higher performance. Withthese two new instruments comes the firststereomicroscope that provides a com-pletely motorized workstation, setting newstandards for our competition in automationand working ergonomy, and once againproving that Leica Microsystems is aninnovator strictly focused on meeting customers' needs.

Leica MZ16 AHigh-performance stereomicroscope with 16:1 zoom, fully apochromatic

73

Research Automated

Range of use:� Research Automated

� Home �

Discover how to gain more informationfaster. As the world's first motorized, auto-mated, fully planapochromatic fluorescencestereomicroscope, the Leica MZ16 FA letsyou control the filter changer, zoom, focus,UV shutter, and the double iris aperture atthe touch of a button, and repeats yourmultifluorescence experiments automati-cally, quickly, exactly, and ergonomically.The Leica MZ16 FA is the fluorescencestereomicroscope with greatest zoomcapability (16:1), highest resolution (840Lp/mm), highest magnification (115x withstandard optics), a patented illumination/filter system for the most intense fluores-cence on jet black backgrounds, and an HLRCTM innovative high-performance trans-mitted-light base for excellent reliefcontrast.

Leica MZ16 FAThe world’s first motorized, automated, fully apochromatic stereofluorescencestereomicroscope

74

Research Automated

Range of use:� Research Automated

� Home �

Our complete individual measurement andinspection stations leave nothing to be desired. The new zoom systems include thewidest line of accessory products to meetevery imaginable examination, training, anddocumentation task. The Leica Z6 APO andZ16 APO are suitable for measuring,documenting and analyzing in the QA lab,just as they are suited for biology, geology,histology, and training.Leica Z16 APO offers a 16:1 zoom with azoom range of 0.57x to 9.2x. The high-magnification Leica Z16 APO is exception-ally well-suited for use in microelectronicsas well as laboratory workstations inmedicine, biology, education, research,development, and criminology.

Leica Z6 APO & Z16 APOHigh-performance zoom systems for perfect documentation

75

Macroscopes

Range of use:� Quality Control� Zoom System

� Home �

The modular zoom systems Leica Z6 APO Aand Z16 APO A correspond to the highestquality standards worldwide and meet allrequirements for first-class documentation,manufacture and inspection. The high-performance fully apochromatic opticsmade of high-quality, multiple-coated,lead-free glasses, in combination with theplan-apochromatic objectives provideparallax-free imaging for authentic, detail-rich image material. But the two zoomsystems are not only exceptional withrespect to the optics, they also excel withrespect to operating comfort at the highestlevel.

Leica Z6 APO A & Z16 APO AHigh-performance zoom systems for perfect documentation

76

Macroscopes

Range of use:� Quality Control� Research Automated� Zoom System

� Home �

Colposcope

The high-performance Leica Colposcopeprovides a maximum of information andconfidence for gynecological diagnosis, ata very competitive price, and represents agood long-term investment.

Not available in the US!

Leica ColposcopeStep or zoom stereomicroscope with illumination

77

Range of use:� Colposcope

� Home �78

� Home �

Imaging SystemsImaging Systemswww.leica-microsystems.com/Imaging_Systems

http://www.leica-microsystems.com/Imaging_Systems

� Home �

The Leica CW4000 total imaging solutioncombines a number of cytogenetics imagingapplications with Leica microscopes andcameras to offer a flexible cytogeneticsimaging system. Leica CW4000 comes in avariety of configurations, offering appli-cation solutions for Karyotyping, FISH,MFISH and CGH analysis. Applicationsrange from karyotyping human metaphasesin clinical applications to non-human karyo-typing in research institutes as well as offering professional solutions for experi-ments such as advanced multicolor FISHassays in cancer and oncology studies.

Leica CW4000High resolution imaging system for cytogenetics

80

Cytogenetic Workstation

Range of use:� Cytogenetics workstation

� Home �

The Q550 MW materials workstation is animaging solution that integrates automatedmicroscopy, computing and digital imageanalysis to increase laboratory productivityby efficiently performing routine, yet so-phisticated analytical tasks, accurately andautomatically.

Sample applications:– Steel inclusion rating– Hardness testing– Quality testing– Coating analysis– Automotive inspection– Filter inspection

Leica Q550 MWFully optimized for materials science and analysis

81

Materials Workstation

Range of use:� Quantitative Imaging� Materials Imaging

� Home �

Leica QClean is a quality assurance ima-ging solution running on the establishedLeica MW image analysis workstation.Leica QClean controls Leica microscopesystems for the automated measurementand classification of particles on circularshaped samples, such as filters in qualityassurance. The system automaticallymeasures filters where the cleaning fluid ofmicro mechanic and engine componentshas been poured through, and dried in anoven. The size and number of particlesmeasured on this filter are used for charac-terizing the cleanliness of the componentsto standard quality requirements defined bythe customer. Furthermore, Leica QClean isfully compatible with most industrialstandards including VDA 19 and ISO 16232.

Leica QCleanAnalysis software for the measurement and classification of particles on filters

82

Materials Workstation

Range of use:� Materials Imaging

� Home �

Leica FW4000 Application Modules aredesigned as an easy to use, modular (by application) fluorescence imaging solution.The modularity of Leica FW4000 makes itapplicable for both entry level and compleximaging solutions. The system is designedto be highly versatile in its choice of com-ponents and software facilities, making itsuitable for a wide range of applications.Each Leica FW4000 Application Moduleis flexible and intuitive for professionalfluorescence imaging.

Leica FW4000Fluorescence imaging

83

Fluorescence Workstation

Range of use:� Fluorescence workstation

� Home �

Leica QWinImage processing and analysis software

84

Imaging Workstation

Leica QWin is a highly versatile imageanalysis and processing solution for quanti-tative microscopy which provides completecontrol of Leica microscopes, macroscopesand Leica digital cameras. The modular andscaleable nature of Leica QWin is such thatits capability ranges from simple interactiveimage measurements to automatic, multiparameter measurements of an immensenumber of features. Leica QWin is availablein 5 editions including QWin Runner, QWinLite, QWin Plus, QWin Standard and QWinProfessional. Each edition is designed tomeet a variety of needs and offers profes-sional upgrade options as your requirementsgrow.

Range of use:� Quantitative Imaging

� Home �

The new Leica Application Suite integratesLeica automated microscopes, digital cam-eras and software into one commonmicro-imaging environment to provide aneasy to use and consistent platform.Leica Application Suite solves andaccelerates routine and research analysis.The rich image processing functions makeit suitable for a diverse range of imagingtasks such as visualization, enhancement,measurements, and documentation. Addi-tional modules can be added to furtherenhance the powerful functionality of LASsuch as Image Overlay, MultiFocus,MultiTime, MultiStep, Extended Annotation,Interactive Measurement, and Reticulerequirements.

Leica Application SuiteDemanding microscopy

85

Imaging Software

Range of use:� Imaging Software� Quantitative Imaging

� Home �

The need for professional, customer speci-fic digital data management solutions hasnever been greater – particularly in view ofthe increasing use of digital cameras. LeicaMicrosystems introduces an improvedversion of Leica Image Manager, aversatile image management applicationfor the acquisition, processing, measure-ment and output of images as well as fordata exchange and data backup. Themodular concept of Leica IM also enablesthe user to specify a system that is tailoredto each individual's specific needs and withspecial networking options, all require-ments from small local networks to largecompanywide systems can be linked.

Leica IM1000Digital data management solution

86

Imaging Software

Range of use:� Imaging Software

� Home �

Digital technology has opened up possibili-ties for turning stereo-pairs into real three-dimensional images that can be viewed andmeasured from different perspectives. Themodular Leica Stereo Explorer softwarepackage is the perfect complement to theLeica IC3 D stereo camera for accurateimaging of the three-dimensional surfacesof the specimens being examined. From thetwo-dimensional stereopairs, Leica StereoExplorer automatically calculates a 3D datarecord that can be viewed and analyzedusing a computer. The realistic image,which appears in relief, makes it easier for the user to identify complex surfaces, greatly improves education and training,and enables better diagnoses in technical,biological and forensic fields.

Leica Stereo Explorer3D views of surfaces as you have never seen before

87

Imaging Software

Range of use:� Imaging Software� 3D Display System

� Home �88

� Home �

Camera SystemsCamera Systemswww.leica-microsystems.com/Camera_Systems

http://www.leica-microsystems.com/Camera_Systems

� Home �

The Leica EC3 is an affordable high speeddigital color camera that offers fast, real-time imaging of up to 15 frames per second.When combined with a Leica microscopeand used in conjunction with the includedLAS EZ software, it is the perfect solutionfor performing a variety of imaging taskssuch as annotations, calibrations, andimage measurements.

The real time speed of the Leica EC3camera means that training on microscopictechniques is now effortless. Live or cap-tured images can optionally be displayed infull-screen mode on a computer monitor toensure visibility. Furthermore, the LeicaEC3’s high 3.1 megapixel resolution producesexcellent images, which makes the camerathe perfect choice for visual presentation.

Leica EC3Affordable high speed digital color camera

90

Digital Photo


� Home �

The Leica DC150 digital camera systemconnects directly to your microscope tocombine the best elements of micro- andmacro-photography. Sporting a finely fash-ioned compact anthracite metal body, theLeica DC150 has 8 million pixels and up-to-the-minute digital features: optical 3.6x and digital zoom, innovative digital signalprocessing, compatibility with SD memorycard, USB interface, and a wealth of otherimpressive functions. With the new soundrecording feature, up to one minute ofspoken notes can be saved per image.

Leica DC150Digital color camera

Digital Photo

Range of use:� Various

91

� Home �

The DFC290 is a color Digital FireWireCamera that offers real-time live imagesthat can be focused and orientated directlyfrom a computer. The 3 Megapixel CMOSsensor digitizes information of the sensor inthe camera head, leading to optimum noisesuppression and detailed images. All colorrenderings, image geometry and dimen-sions are correct to ensure accurate resultsare obtained during image analysis andimage processing. The Leica DFC290 iswholly compatible with Leica’s micro-scopes and Leica software to form anintegrated and powerful imaging system formicroscopy applications. Combining theLeica DFC290 with the new LAS Modulesprovides a sophisticated solution for criticalanalysis and documentation. The camera iscompatible with PC/Mac software and isdesigned to simplify the imaging processfrom capture through to processing.

Leica DFC290Powerful digital color camera system for real-time imaging

92

Digital Photo


� Home �

Digital Photo

The Leica DFC300 FX Digital Color Cameraspecializes in high sensitivity imaging influorescence for genetic research, biotech-nology and medicine. The Leica DFC300 FXdigital camera records live cells, sequencesof motion and fluorescence specimens orparticles that are susceptible to photo-bleaching, even at the lowest light inten-sities. High sensitivity in the visible spectrumensures reliable results in fluorescencemicroscopy, especially for GFP and otherlow illumination applications. The stylishcamera housing is lightweight and compact, and easily attaches to your particular microscope (c-mount). The LeicaDFC300 FX camera also provides quicktransfer for PC and Macs with standard FireWire interface.

Leica DFC300 FX Digital color camera for fluorescence applications


93

� Home �

Digital Photo

The high dynamic range offered by theLeica DFC340 FX allows dark and lightobjects (monochrome) to be recorded andevaluated within the same image. The 2-megapixel CCD sensor in the digital cameraensures that each image reveals extra-ordinary detail for the most critical publica-tion quality. The progressive scan readoutmode provides full resolution in every liveand captured frame. A variety of binningand readout modes adapt to both bright anddim signals in the images to ensure nearreal time live speeds while reducing the risk of photobleaching the most sensitivesamples.

Leica DFC340 FX Specifically designed for imaging applications with limited light

94


� Home �

Digital Photo

The Leica DFC350 FX monochrome DigitalCamera is specially designed for fluores-cence analytical imaging requirements. Featuring 1.4 megapixel resolution and a FireWire interface, it records live cells, sequences of motion and fluorescencespecimens or particles that are susceptibleto photobleaching, even at the lowest lightintensities. High sensitivity in the visibleand infrared spectrum ensures reliable results in fluorescence microscopy, especially for GFP and other low illumi-nation applications. The stylish housing is lightweight and compact, and easily attaches to your particular microscope (c-mount).

Leica DFC350 FX Monochrome digital camera for fluorescence applications

95


� Home �

Digital Photo

Excellent picture quality is essential forprecise image analysis, documentation andreporting. The 5 megapixel Leica DFC420Digital Camera system with multishot tech-nology and FireWire interface provideshigh-resolution pictures with outstandingdetail accuracy and brilliant color repro-duction. The Leica DFC420 is the cost-effective alternative to traditional filmphotography and analog video camera systems. Exceptional picture quality andease of use make the Leica DFC420 the perfect choice for precise, fast imaging fordocumentation and analysis.

Leica DFC420Digital color camera (c-mount) for all applications

96


� Home �

Digital Photo

The DFC420 C is a high resolution DigitalFireWire Camera with integrated Peltiercooling that allows crisp, sharp images tobe created without noise, even under lowillumination. The advanced 5 megapixelsensor digitizes information of the CCD chipin the camera head, leading to optimumnoise suppression and highly detailedimages. Exceptional picture quality and easeof use make the Leica DFC420 C a perfectchoice for brightfield, darkfield and phasecontrast microscopy in life science, indus-trial and clinical applications. The LeicaDFC420 C is compatible with Leica’s micro-scopes, stereomicroscopes and macro-scopes and Leica software to form anintegrated and powerful imaging system formicroscopy applications. Combining theLeica DFC420 C with the new LAS modulesprovides a sophisticated solution for routineand research analysis.

Leica DFC420 CDigital FireWire color camera for analysis & documentation

97


� Home �

Digital Photo

New applications in life science and industry require innovative approaches toimaging. Quickly producing high qualityimages for documentation, evaluation andanalysis is a key factor for imaging success. The Leica DFC490 digital camerasystem with multishot technology and FireWire interface provides images for thehighest color fidelity, resolution and detail.Real time speeds can be achieved using anarray of innovative read-out modes.

The innovative Leica DFC490 integrates an8 Megapixel CCD, which offers superiorquality, ultra high resolution images thatwere previously only possible with multipleacquisition cameras. High resolution CCD'sare especially beneficial for low magni-fication imaging on microscopes as theamount of information provided by theoptical system is much larger than in highmagnification conditions.

Leica DFC490Digital color camera (c-mount) for all applications

98


� Home �

Digital Photo

The Leica DFC500 digital camera systemallows versatile use of all modern microsco-pic procedures in research, development,medicine, science and industry. LeicaDFC500 digital cameras, with 12 megapixelsand a color depth of 42 bits RGB, takedifficult pictures of objects in extremelypoor light, e.g. with weak fluorescence.

Leica DFC500High resolution digital camera system

99


� Home �

Digital Photo

Leica Microsystems is proud to producethe most complete 3-D microscopic imagingsystem. Images that could previously onlybe visualized through stereoscope eye-pieces can now be captured and displayedelectronically. Starting with a high-perfor-mance stereo-microscope, we add a dual-chip digital camera which can produce atrue 3-D image on screen for training pur-poses and capture these images for furtherprocessing to reveal specimen measure-ment data such as profile, surface area,and volume. The complete 3-D picture ofmicroscopic specimens, from eyepiece toon-screen to topographical measurements,is now at your fingertips.

Leica IC 3DDigital color camera for M stereomicroscopes

100


� Home �

The Leica IC A is an ergonomic, reasonablypriced, high-performance video camera forLeica M stereomicroscopes and for the Leica Colposcope.

The Leica IC A opens up new horizons in industrial quality control, in the interpre-tation of thin section images, in education,in medicine, in the demonstration of imagesto large groups of people, and in digitalpost-processing.

Leica IC AIntegrated video module for M stereomicroscopes

101

Analog Photo


� Home �

Analog Photo

The Leica ICC A is an ergonomic, reasonably-priced, high-performance video camera forLeica DM L microscopes. The Leica ICC Aopens up new horizons for the image analysis of thin sections in science, foreducation, medicine, industrial qualitycontrol, as well as for training, live presen-tations to large audiences, and digitalpost-processing.

Leica ICC AAnalog video camera for compound microscopes (DML series)

102


� Home �

Surgical MicroscopesSurgical Microscopeswww.leica-microsystems.com/Surgical_Microscopes

http://www.leica-microsystems.com/Surgical_Microscopes

� Home �

All important and necessary patient datacan now be displayed in the LeicaM525/M520/M500N optical platform on theLeica OH4, OH3, MS3 or F40 with a multi-tude of benefits: highest brightness, contrast >300:1, true color and 1024x768 pixel resolution.

The new Master-I-ViewTM function allowsthe surgeon to observe the data with hisdominant eye, left or right. Leica’sQuad-Shutter TechnologyTM automaticallycontrols the appearance of different datatypes by taking care of the individual setup.A lightsaving, ergonomic beamsplitter design rounds off the outstanding functio-nality of this new Leica module.

Leica DI C500Digital imaging color module for surgical microscopes

104

Accessories

Range of use:� Neurosurgery� Orthopaedic Surgery� Plastic/Reconstructive Surgery� Documentation System

� Home �

The Leica ULT500 (Leica Ultra Observer) isthe new lightsaving solution for completeand flexible co-observation for all appli-cations with the M525/M520/M500N opticscarriers as in neurosurgery, spinal surgeryor ENT.

A compact design allows better ergonomicsfor the surgeon and the assistants, theobservation ports can easily be switched.Modular like all Leica accessories, the Leica Ultra Observer can be mountedeasily and directly to any Leica OH4, MS3 orF40 as well as to any previous model suchas Leica OH3, OHS1, MS2 or MS1.

Leica ULT500Ultra observation unit for surgical microscopes

105

Accessories

Range of use:� Neurosurgery� Orthopaedic Surgery� Plastic/Reconstructive Surgery� Documentation System

� Home �

The Leica Zoom Video Adapter producesimages with a previously unobtainablebrilliance and natural color intensity.Changing the magnification and refocusingfor the most successful documentation isnow possible, in standard situations or inteaching.

The Leica Zoom Video Adapter fits indivi-dual requirements. Whether the surgeonalready has or wants to acquire a 1-chipCCD or a 3-chip CCD camera with C-mount,Leica’s Zoom Video Adapter integrates perfectly to compensate varying light intensities of any available screens and video cameras.

Leica Zoom Video AdapterZoom video adapter with integrated fine focus and neutral density filter

106

Accessories

Range of use:� Ophthalmic Surgery� Neurosurgery� ENT Surgery� Orthopaedic Surgery� Plastic/Reconstructive Surgery� Veterinary Surgery� Documentation System

� Home �

Surgical Microscopes

We believe that excellent diagnosis is justas important as a successful operation.That’s why we designed the Leica M300 tothe same high quality specifications as ourreputed surgical microscopes. The result:images of a standard you won’t find in anyother diagnostic microscope.

Although the Leica M300 naturally does nothave all the features of a surgical micro-scope, it shows you more diagnostic detailsthan any conventional product. There is noother diagnostic microscope offering any-where near the same light intensity andimaging quality.

Leica M300Diagnostic microscope system with surgical optics and 5-step magnification

107

Range of use:� ENT Surgery� Gynaecologic Surgery� Dental Surgery� Veterinary Surgery

� Home �


The Leica M400 E offers brilliant optics withoutstanding field depth and superlativecontrast. Brought straight to the operatingtable with the long swivel arm, the LeicaM400 E incorporates Leica’s patentedone-hand-movement system, an extremelysmooth balancing system for reliably fastand precise positioning.

Besides the integrated halogen illuminationwith coaxial light beam there is also anoptional light intensifier or a xenon lightsource. The standard configuration has awide range of accessories for sharedviewing and documentation. Thanks to itscompact design, surgery is more comfor-table and more efficient.

Leica M400 ESurgical microscope for ENT surgery, with 5-step magnification

108

Range of use:� ENT Surgery� Gynaecologic Surgery� Dental Surgery� Veterinary Surgery


The new Leica M525 F40 provides uniquesupport for neurosurgeons, ENT, and spinalspecialists during surgery. Leica’s M525OptiChromeTM premium optics and thecompact Leica F40 stand are the perfectanswer to the challenges of microsurgery.

Best viewing, perfect balance, easy mobili-ty, optimal stability and excellent value formoney all define this new Leica microscopesystem. The slim design of the Leica F40stand conceals innovative engineering thatprovides a unique homogeneity of movement.Sophisticated interface solutions make theLeica M525 F40 compatible with neuro-navigation/IGS systems.

A new feature of the Leica M525 micro-scope is AutoIrisTM – the coupling of theillumination brightness control to theworking distance for even more reliablework at short distances.

Leica M525 F40Surgical microscope system for neuro-, ENT-, & PRS surgery

109

Range of use:� Neurosurgery� ENT Surgery� Orthopaedic Surgery� Plastic/Reconstructive Surgery� Veterinary Surgery

� Home �

� Home �

Leica M525 MC1Surgical microscope system for neuro-, ENT-, & PRS surgery

110


The Leica M525 MC1 features the premiumOptiChromeTM optical system. It standssecurely on the floor and can be positionedquickly and precisely. Its unique balancingsystem provides the best possible supportfor ENT surgery, neurosurgery and a varietyof multidisciplinary applications. LeicaErgonOpticTM offers a range of observationoptions.Standard features include a 300 W high-performance xenon lamp, motorized 6:1zoom and motorized focus via multifocallens from 207 mm to 470 mm – both withvariable speeds. The intelligent ISUS setupsystem supports custom configurations andfast responses by the surgical team thanksto the autodiagnostic function.A new feature of the Leica M525 micro-scope is AutoIrisTM – the coupling of theillumination brightness control to the work-ing distance for even more reliable work atshort distances.

Range of use:� Neurosurgery� ENT Surgery� Orthopaedic Surgery� Plastic/Reconstructive Surgery� Gynaecologic Surgery� Dental Surgery� Veterinary Surgery

� Home �

The Leica M525 MS3 is a robotic stand withpremium OptiChromeTM optics for neuro-,spine surgery and ENT. Due to the sixelectromagnetic brakes, the stand caneasily be moved in six axes and is alsocombined with robot movements in threeaxes.New, easy-grip handles of robust castmetal have been designed for ergonomicpositioning. Each handle has an easy-to-operate joystick to activate the motorizedXY adjustment of the optics carrier. A 150°inclination range combined with an ultracompact optical microscope allows thesurgeon to adjust the position of the micro-scope comfortably even during extremelydifficult operations. Ready for XY-tool-tracking with IGS.A new feature of the Leica M525 micro-scope is AutoIrisTM – the coupling of theillumination brightness control to theworking distance for even more reliablework at short distances.

Leica M525 MS3Surgical microscope system for neurosurgery

111


Range of use:� Neurosurgery� ENT Surgery� Orthopaedic Surgery� Plastic/Reconstructive Surgery

� Home �

Designed and manufactured using superiormaterials and the highest quality standards,the premium Leica M525 OH4 is built forlong service life and outstanding reliability.The Leica M525 OH4 fulfils Leica's vision ofproviding the best viewing conditions andthe greatest maneuverability to enablesuccessful surgery.

The Leica M525 OH4 stand not only comple-ments Leica's M525 optics, but also adds tothe overall microsurgical experience withsuperior movement, innovative illuminationand user-friendly features.

Leica M525 OH4Premium surgical microscope system for neurosurgery

112


Range of use:� Neurosurgery� ENT Surgery� Orthopaedic Surgery� Plastic/Reconstructive Surgery

� Home �

The new Leica M620 F18 is a perfect balanceof form and function. Precision optics com-bined with finest mechanics make the LeicaM620 F18 an outstanding choice. Swiss de-sign, quality and precision – the LeicaM620 F18 meets the needs of modern dayophthalmic surgery.The Leica M620 features Leica’s exclusiveOptiChromeTM optics combined with Leica’sexclusive Direct Halogen Illuminationsystem. As a result of more than 25 years ofexperience in developing surgical micro-scopes dedicated to ophthalmology, thenew Leica M620 F18 offers the quality andreliability of a premium surgical microscopeat an attractive price/performance value.The new Leica M620 F18 floorstand is com-pact and moves easily. With the new high-quality precision bearings and a longswingarm, the stand is highly maneuvera-ble and easy to set up for surgery.

Leica M620 F18Floorstand surgical microscope system for ophthalmology

113


Range of use:� Ophthalmic Surgery� Veterinary Surgery

� Home �


High-caliber teaching equipment is a fun-damental prerequisite for the best learningenvironment. Using a high-quality micro-scope under the supervision of a qualifiedspecialist is one of the keys to successfuladvanced education and practice.

The new Leica M620 TTS (table top stand)microscope meets the needs of surgicaltrainees as well as trainers. The M620 TTSmicroscope system offers brilliant resolu-tion, a large depth of field with outstandingstereopsis, and natural color reproductionon a convenient table top stand.

Leica M620 TTSTable top microscope system for ophthalmology

114

Range of use:� Ophthalmic Surgery

The Leica M651 has been specially designedfor use in microsurgery and is well knownfor its remarkably clear, sharp images,great depth of field, pronounced 3D effect,high light intensity, and its faithful colorrendering.

Due to its high perfomance, the Leica M651is also used for various technical, industrialand research applications. The brilliantLeica optics in combination with an easy tohandle and stable floor stand perfectly support the work in all of these fields andoffer an outstanding price performance ratio.

Leica M651High-performance multidiscipline medical microscope as well as non-medicalstereomicroscope, with 5-step magnification

115


Range of use:� Gynaecologic Surgery� Veterinary Surgery

� Home �

� Home �

The table top model of the field provenLeica M651 surgical microscope offersbeginners ideal conditions for practicingmicrosurgical techniques. It is equallysuitable for advanced surgeons who wantto refresh their skills.

The Leica M651 MSD is well known for itspeak performance in image definition,depth of field range, stereopsis, lightintensity and color rendering: ideal condi-tions for prospective surgeons to get usedto their own hand movements in amagnified image, learn to differentiatebetween minuscle features and learn touse the finest surgical thread available.

Leica M651 MSDTable top microscope for teaching and practice

116


Range of use:� Ophthalmic Surgery� Veterinary Surgery


The unique “two-in-one”surgical microscopeLeica M680 has been specially designed toprovide ideal conditions for two surgeonsworking together. Leica’s unique 2x2 formulais represented by two independent zoomsystems and two independent focusingsystems with a remarkable depth of fieldand an outstanding wide field of view.

Besides conventional operating, one pushof a button couples the two magnificationchangers, allowing solo operations to be performed with the Leica M680 while retaining the individual focusing facilities.

Thanks to Leica M680 you simply see betterand more.

Leica M680Dual surgical microscope

117

Range of use:� Orthopaedic Surgery� Plastic/Reconstructive Surgery� Gynaecologic Surgery� Veterinary Surgery

� Home �

� Home �

With the Leica M820 F19 high performancemicroscope with OptiChromeTM optics forophthalmic surgery Leica Microsystemsexpands its product range of the M8 seriesfor demanding ophthalmology.

The combination of excellent optics withcomfortable handling and easy maneuvera-bility of the Leica F19 compact stand setsthe Leica M820 F19 apart as a surgicalmicroscope with an outstanding priceperformance ratio.

Leica M820 F19Surgical APO microscope system for ophthalmology

118



� Home �

The Leica M844 with its Leica F19 standcontains four high precision mechanicalfriction brakes for homogenous arm movement and high operating stability. The Leica M844 F19 carries the original APO OptiChromeTM optics, stands on the same small footprint and holds the sametwo-in-one display and XY unit as the LeicaM844 F40.


119



� Home �

The Leica M844 F40 is the premium classsurgical microscope system for ophthal-mology. Optimal lighting conditions are provided by the Leica direct halogen illumi-nation system for improved contrast andRed Reflex.

The original APO OptiChromeTM optics inconjunction with the Quad-ZoomTM zoomsystem guarantee optical brilliance. APOOptiChromeTM, in combination with theLeica F40 floor stand, are the ideal answersto the challenges of ophthalmic microsurgeryin the 21st century.

The Leica M844 F40 provides the bestpreconditions for safe, fatigue-free work –regardless of the physical size and postureof the user.


120



The Leica 2D video system offers you twosmart solutions for microsurgical videoimaging: The Leica 2D pick-up system, anintegrated camera module to fit on all LeicaM series and the Leica 2D C-mount for aninstallation with standard video adapters.

The compact minimal-height design ensuresoptimal ergonomic conditions. The separate2D camera control unit provides you with aBNC, S-Video and RGB output signal andensures that all commercially available TVmonitors can be used with the system.

Leica 2DCompact camera module for microsurgical applications

121

Video Recording System

Range of use:� Ophthalmic Surgery� Neurosurgery� ENT Surgery� Orthopaedic Surgery� Plastic/Reconstructive Surgery� Veterinary Surgery� Documentation System� Home �

� Home �

Video Recording System

The new Leica MDRS4 is a compact, power-ful digital recording system, specificallydesigned to mount on a surgical microscope.The Leica MDRS4 provides a completelyintegrated yet independent video recordingsystem.

The Leica MDRS4 records directly to a 100 MBhard drive and if desired synchronally to aUSB drive. Within 15 minutes a completeDVD will be burnt. The new Leica MDRS4 isthe ideal solution to create high-qualityvideos and still images for networking,documentation and archiving to patientrecords.

Leica MDRS4Leica medical digital recording system

122

Range of use:� Documentation System

� Home �

Histology Systems andMaterials Sectioningwww.leica-microsystems.com/Histology_Systems

www.leica-microsystems.com/Materials_Sectioning

http://www.leica-microsystems.com/Histology_Systems

http://www.leica-microsystems.com/Materials_Sectioning

� Home �

Comfortable, error-free printing at highresolution. Every Leica machine is designedfor high throughput. This also applies to ournew Leica IP C and IP S ink-jet printingsystems for automated imprinting of micro-scope slides (Leica IP S) and specimencassettes (Leica IP C). The two all-newprinters offer unmatched printing qualitywithout compromising on printing flexibility:one- and two-dimensional barcodes, logos,photographs, and alphanumeric char-acters. All data remain clearly legible as theimprints are chemically and mechanicallyresistant. Furthermore, errors arising frommanual labeling are simply a thing of thepast.

Leica IP C & IP SModular printing systems for cassettes and microscope slides

124

Laboratory Printer Systems

Range of use:� Histology Printing Systems

� Home �

Tissue Processors

The Leica ASP300 S smart, fully enclosedtissue processor is designed for routineand research histopathology. Proventechnology combined with top qualitycomponents and Leica´s RemoteCareTM

diagnostic (optional) provide superior instrument reliability. Straightforwardroutine user operations by an intuitive userinterface, color touch screen and a varietyof “smart” features, such as ReagentManagement System and quick start forcommonly used programs, improve speci-men quality and laboratory economy.

Leica ASP300 SFully-enclosed tissue processor

125

Range of use:� Tissue Processing

� Home �

Peloris is a rapid tissue processor thatcreates high quality results for any tissue. Ithas a xylene-free option and a uniqueActivFloTM system that generates idealprocessing conditions. A range of consum-ables adds extra value with solid Para-blocksTM wax for easy, safe wax transfer,cassettes that eliminate biopsy pads, andreagents to completely eliminate xylene.With Peloris, any laboratory can enjoy theconfidence of high quality results whilemeeting new workflow challenges likereduced turnaround times, Lean productionand same day diagnosis.

Peloris is a high-productivity system that isideal for laboratories pursuing LeanHistologyTM and six sigma principles.

Leica Peloris™Rapid tissue processor

126

Tissue Processors

Range of use:� Tissue Processing

� Home �

Embedding Products

The Leica EG1150 modular tissue embeddingcenter incorporates two separate compo-nents, the Leica EG1150 C cold plate andthe Leica EG1150 H heated paraffin dispen-sing module. The independent modulesoffer the flexibility to arrange embeddingworkflow in either direction (left to right orright to left) to suit the user's needs.

For orienting specimens from biopsies andother, especially very small specimens, anadjustable magnifier is available as anoption.

Leica EG1150Modular tissue embedding system

127

Range of use:� Embedding

� Home �

Embedding Products

Compact dimensions, ease of operation,and excellent standards of convenienceand safety characterize the Leica EG1160paraffin embedding station. The separatelyheated paraffin dispensing system of theLeica EG1160 with an integrated filterensures constant, reproducible paraffinflow at ten different flow rate settings.

All instrument functions – including theautomatic starting time – are individuallyprogrammable at just the press of a button.Large and temperature-controlled handrest areas give the user maximum freedomof movement.

The Leica EG1160 offers a wide range ofaccessories (cold light source, vacuumattachment and magnifier).

Leica EG1160Embedding center, dispenser and hot plate

128

Range of use:� Embedding

� Home � 129

The Leica LN22 liquid nitrogen freezingattachment was designed specifically foruse with the Leica RM2265 rotary micro-tome. Sections of uncompromising qualitycan be obtained from even the mostchallenging specimens in the fields ofindustry and materials research – at sec-tion thickness settings between 0.25 and100 µm and working temperatures as low as-150 °C.With the Leica RM2265, changing fromambient temperature to low-temperaturesectioning and vice versa can be realizedquickly and easily.

Leica LN22Nitrogen freezing device

Microtomes

Range of use:� Sectioning

� Home �

Microtomes

The Leica RM2125 compact rotary micro-tome – the standard for laboratory routine.It is designed specifically for paraffinsectioning applications in routine laborato-ries. Offering the latest in technology at avery attractive price/benefit ratio the focusis set on what is essential without compro-mising on sectioning quality, ease ofoperation and user safety. With our tailor-made range of optional accessories, youcan set up these instruments in virtually anyconfiguration for both routine and specialapplications.

Leica RM2125Manual rotary microtome

130


� Home �

The Leica RM2235 is a robust rotary micro-tome designed for manual sectioning ofprimarily biological specimens embeddedin paraffin. Low-maintenance cross rollerbearings for horizontal and verticalspecimen movement ensure accuratereproducibility of the section thickness andoptimum quality even when sectioning hardtissues. It boasts numerous featurespreviously unavailable in this microtomeclass. The Leica RM2235 comes in twodifferent basic versions and in differentconfigurations to meet all current userrequirements in the field of clinicalhistopathology.

Leica RM2235Manual rotary microtome

131

Microtomes


� Home �

The RM2245 is a semi-motorized rotarymicrotome, designed for routine andresearch applications in histology, histo-pathology and industrial quality assurancelaboratories. Manual sectioning is enhancedby the high-precision motorized specimenfeed, which results in efficient operationwith maximum section reproducibility. Withthe Leica RM2245 there is a choice betweenthe conventional manual sectioning methodof full handwheel rotation and the "rockingmode". In rocking mode, the handwheel isonly turned back and forth over a shortdistance. The RM2245 comes with a holdersystem for disposable blades, a size-optimized control panel and a precisionspecimen orientation with clear zeroreference point.

Leica RM2245Semi-motorized rotary microtome

132

Microtomes


� Home �

The Leica RM2255 rotary microtome,designed for fully motorized sectioning ofboth paraffin- and resin-embedded speci-mens, offers a broad application spectrumin routine and research laboratories inhistology as well as in industrial materialsand quality control. Its two-in-one designconcept, which allows motorized as well asmanual sectioning, provides reproduciblequality sections. The entire accessory lineincluding knife holders, specimen clampingsystems and specimen orientation devicehas been re-engineered providing the mostsuperior technology available on themicrotomy market.

Leica RM2255Fully motorized rotary microtome

133

Microtomes


� Home �

The Leica RM2265 is Leica’s top-of- the-linefully motorized and programmable rotarymicrotome designed primarily to satisfy therequirements of biomedical researcherssectioning hard to semi-soft materials andcustomers needing to section industrialmaterials for quality assurance and materi-als defects analysis. The RM2265 is also theinstrument of choice for sectioning at ultra-low temperatures (-150 °C) in combinationwith the Leica LN22 liquid nitrogen freezingattachment.

Leica RM2265Fully motorized rotary microtome

134

Microtomes


� Home �

The Leica SM2000 R sliding microtome withlow-maintenance cross-roller-bearing guide-ways is designed to section paraffin-embedded samples in routine histopathologylaboratories. The SM2000 R can also beused for the specific industrial applicationsof foam and wood sectioning. The easy-to-use knife holder is designed to accommo-date both standard knives and disposableblades. The instrument is available fullyequipped to meet most of today's slidingmicrotome sectioning requirements or canbe customized to meet more specialapplications.

Leica SM2000 RSliding microtome

135

Microtomes


� Home �

Designed for biomedical and industrialapplications, the Leica SM2400 is a univer-sal instrument for a variety of applicationsin all laboratories where paraffin or variousindustrial materials need to be sectioned.Its sturdy construction lends it qualities thatensure excellent sectioning results. Thesafety features of the system, such as asledge locking device and knife guard aresome of the outstanding features toguarantee convenient operation.

Leica SM2400Sliding microtome (paraffin, plastics)

136

Microtomes


� Home �

Superior system for sectioning and surfacepreparation of large and hard specimens.

The Leica SM2500 is the microtome of choicefor all sectioning applications involvinglarge and hard specimens. You can confi-gure the Leica SM2500 for applications inboth biomedicine and industry using thewide range of accessories.

In addition, the Leica SM2500 can be fittedwith the optional ultramilling attachmentLeica SP2600 for preparation of particularlyhigh-quality specimen surfaces.

Leica SM2500 & SP2600 UltramillerHeavy-duty sliding microtome with separate electronic control unit

137

Microtomes

Range of use:� Sectioning� Ultramilling

� Home �

Microtomes

The Leica SP1600 saw microtome is designedfor cutting hard and brittle materials suchas bone and teeth embedded in methylmethacrylate with or without implants witha maximum size of 35 mm in diameter.Implant materials such as steel, titanium orbioceramics cause no problems for theLeica SP1600 saw microtome. It is used instructural bone research laboratories,dental institutes and various industriallaboratories. The specimen is clamped inthe center of a diamond-coated internal-hole saw and pushed by a spring mecha-nism against the saw blade which rotateshorizontally at a speed of 600 rpm, produc-ing specimen slices of the desired thick-ness.

Leica SP1600Saw microtome

138


� Home �

The Leica SP9000 knife sharpener is designedfor resharpening c-profile microtome knivesup to 22 cm in length, giving a used knifeedge the sharpness of a new or factory-re-conditioned knife. The knife is resharpenedby clamping it in the knife holder at a fixedangle. The transparent plastic cover of theinstrument is closed during the honingprocedure to ensure safe operation and toavoid dust and grinding particles beingscattered over the work place.

Leica SP9000Automatic knife sharpener

139

Microtomes


� Home �

The Leica VT1000 S is a vibrating blademicrotome, designed specifically to meetthe highly sophisticated sectioning require-ments in the fields of neurophysiology,neuropathology and experimental (brain)research – such as patch-clamping technique– and some industrial applications relatedto structural analysis of foam and othervery soft materials.

The Leica VT1000 S can easily be adapted,depending on the application, by alteringthe frequency between 0 and100 Hz and byselecting the appropriate amplitude (0.2 to 1mm in 5 steps). The instrument features avery fine adjustable knife advance speedbetween 0.025 to 2.5 mm/s and programm-able sectioning window and specimenretraction.

Leica VT1000 SVibrating blade microtome

140

Microtomes


� Home �

The Leica VT1200 and VT1200 S vibratingblade microtomes are designed for section-ing of unfixed tissue specimens. The LeicaVT1200/S microtomes feature a bladeholder design that minimizes verticaldeflection of the blade and protects delicatespecimens, such as brain, spinal cord andother mammalian tissues, from mechanicaldamage. Instrument stability and minimalvertical deflection result in sections of thehighest quality, while retaining viable cellson the section surfaces in greater numbers.

The semi-automated Leica VT1200 is de-signed for users who prefer to control thesectioning parameters manually, whereasthe Leica VT1200 S is the fully automatedand recommended model for multi-userlaboratories.

Leica VT1200 & VT1200 SVibrating blade microtome

141

Microtomes


� Home �

State-of-the-art technology and perfectuser friendliness characterize the largeLeica cryostats – distinctive features whichare also available in a compact, portabledesign. With the need for mobile diagnos-tics ever increasing, the Leica CM1100 isthe ideal cryostat for safe and fast on-the-spot evaluation – at the same time it is alsoan excellent back-up instrument. With atotal weight of only 50 kg, the Leica CM1100can be easily carried and be transported inpractically any vehicle.

Leica CM1100Bench top cryostat for clinical pathology

142

Cryostats


� Home �

The Leica CM1510 S is a cryostat whichreliably meets all demands in routine cryo-microtomy and excels by outstandingeconomic efficiency: a very attractively priced instrument which leaves nothing to be desired in its class; compact dimensions – especially advantageous inlaboratories where space is limited; highspecimen throughput for rapid and efficientperformance, and a proven track record ofreliability. And of course – just as any otherLeica product – the Leica CM1510 S offersexemplary safety standards and is capableof fulfilling even the most exacting operatorrequirements for excellent ergonomics.

Leica CM1510 SCryostat for routine histology

143

Cryostats


� Home �

The Leica CM1850 cryostat for standardapplications in the clinical histopathologylaboratory is designed for easy use and ahigh-volume workload. The insulation tech-nology used enhances the durability of therefrigerating system and maintains stablecryochamber temperatures even whenoperating the instrument under unfavorableenvironmental conditions.

Leica CM1850Cryostat for clinical applications

144

Cryostats


� Home �

The Leica CM1850 UV incorporates theproven excellent features of its predeces-sor – and much more. It is equipped with anoptimized refrigeration system affordingextremely short specimen freezing times. The cryochamber can be disinfected quick-ly and effectively at any time with ultra-violet light (UVC) for increased operatorsafety. UV surface disinfection significantlyreduces the danger of infection by bacteria,spores and viruses.Leica’s new, antimicrobial nano-silvercoating, AgProtectTM, provides outstandingprotection to cryostat users by reducingexposure to surface pathogens. Leica’sAgProtectTM covers the cryostat’s outsidesurfaces and protects the operator and otherindividuals in the work area by penetratingthe membranes of microbes to preventreplication. The outstanding safety of theCM1850 UV has been tested and certifiedby an independent laboratory.

Leica CM1850 UVCryostat for routine histology

145

Cryostats


� Home �

The versatile and uncomplicated use of theLeica CM1900 as well as the reliable resultsobtained make it the instrument of choice inmany routine as well as advanced cryostatapplications. The open top stainless steelcryochamber with separate specimencooling is easily accessible and offersample space for convenient working andstorage of specimens. The cryostat can beeasily disinfected with the Leica Cryofectspray at a temperature of around -20° C.

Leica CM1900Rapid sectioning cryostat for routine diagnostics

146

Cryostats


� Home �

The Leica CM1900 UV comes with excellentreferences: the efficient germicidal effectof UVC rays for surface disinfection in theLeica CM1900 UV cryostat has been provenby an independent laboratory. Expertopinion furnishes detailed information onthe test procedure and the efficacy of themethod. The procedure is ozone-free andnon toxic for the user. Userfriendly selec-tion between short and long term exposurecan be initiated without the need ofchanging the operating temperatures. UVCsurface disinfection is effective at ambient,mid or low range temperatures and can beterminated to allow urgent incoming casesto be immediately sectioned.

Leica CM1900 UVRapid sectioning cryostat for routine diagnostics

147

Cryostats


� Home �

The Leica CM3050 S cryostat was designedprimarily for the demanding needs of cryo-sectioning systems in the field of biomedi-cal, neuro-anatomical and pharmaceuticalresearch. Particularly when working withdelicate specimens – for example brainsamples in neuroscience – the precisespecimen orientation and the specimenfeed system via step motor guaranteesreproducible thin serial sections of maximumquality. The Leica motorized CM3050 S isavailable with or without active specimencooling. An ergonomic height adjustmentcan be retrofitted, enabling the user tooperate the cryostat in a sitting or standingposition.

Leica CM3050 SResearch cryostat

148

Cryostats


� Home �

The Leica CM3600 XP is a fully computer-ized cryomacrotome for whole-body sec-tioning primarily in the fields for quantitati-ve investigation of the effect of labeledcompounds, pharmaceuticals and (bio)-chemicals in pre-clinical studies, as well asdetailed anatomical and morphologicalanalysis.

The Leica CM3600 XP instrument softwarebased on Windowsxp allows the creationand recording of GLP compliant documen-tation.

Sections of reproducible thickness can beproduced with the sturdy heavy duty micro-tome and directly dehydrated in the largestainless steel cabinet. The standard knifeholder allows sectioning of specimens ofup to the size of 450x150x160 mm (LxWxH).

Leica CM3600 XPCryomacrotome

149

Cryostats


� Home �

Stainers, Coverslippers

The Leica CV5030 fully automated glasscoverslipper produces slides with superioroptical quality for reliable long-term storage.It is designed to coverslip microscope glassslides bearing tissue sections or cytopatho-logy smears. The capability of handling alarge variety of slide racks from differentsuppliers makes the Leica CV5030 a mostflexible instrument. All common mountingmedia, including xylene-free varieties, canbe used according to individual operatorsettings. The system is capable of high-throughput coverslipping of more than 500slides per hour using standard microscopeslides and glass coverslips of differentsizes. The operator can choose betweenwet and dry coverslipping.

Leica CV5030Robotic coverslipper

150

Range of use:� Coverslipping

� Home �

The Leica ST4040 Linear Staining System isdesigned to meet the current and futureneeds of today's high throughput staininglaboratories. Routine applications such asH&E can be run at a throughput capacity ofup to 1000 slides per hour. The modulardesign of the system allows the customerutmost flexibility in instrument configura-tions to meet the needs in both stainingvolume and workflow of histopathology andcytopathology laboratories. In addition,optional loading and unloading stations areavailable to further increase capacity.

Leica ST4040Linear stainer

151


Range of use:� Staining

� Home �

The Leica Autostainer XL is a robotic stainingsystem for performing all standard routinestaining methods of thin sections mountedon glass slides. The system is a fully self-contained bench top unit with 18 reagentstations, an integrated oven and 5 washstations with flow control valves.Continuous loading and unloading of slidecarrier racks ensures that the instrument isflexible even when the workload is high.

In addition, the Leica ST5010 can accom-modate up to 11 slide racks containing 30slides each, processing at least 200 slidesper hour, depending on the program run.

Leica ST5010Autostainer XL

152



� Home �

With the Leica ST5020 Multistainer, a newlevel of performance is introduced to thepathology laboratory. This new system wasdesigned to achieve perfect staining resultsand enable modern histology and cytologylaboratories to experience an unsurpassedlevel of flexibility. Both routine and specialstaining protocols can be performed aloneor in combination to produce consistentand high-quality staining results.

The Leica ST5020 features a unique userinterface built around a solvent-resistantcolor touch screen.

Based on a modular design principle, theLeica ST5020 can be easily set up to meetindividual laboratory needs.

Leica ST5020Multistainer

153



� Home �

The Leica TS5015 Transfer Station interfacesthe Leica Autostainer XL (ST5010) with theLeica CV5030 Coverslipper.

The Leica ST5010 is the solution for auto-mated, routine slide staining in histologyand cytology. Since its introduction, it hasbeen technically enhanced and made inGermany according to the highest manu-facturing standards. With the availability ofthe CV interface, thousands of laboratoriesnow have the unique opportunity toupgrade to a walk-away system andsignificantly reduce hands-on time.

The simple operation makes the CV5030easily adaptable to the varying require-ments of routine samples, such as histologysections, cytology smears or monolayerpreparations.

Leica TS5015Transfer station to automate the staining and coverslipping processbetween ST5010 (Autostainer XL) and CV5030

154



� Home �

The Leica TS5025 Transfer Station interfacesthe Leica ST5020 (Multistainer) with theLeica CV5030 Coverslipper. Both instru-ments can be combined to set up anintegrated, fully automated staining/coverslipping workstation. Together, theLeica ST5020 Multistainer and the CV5030Coverslipper create a highly flexible systemfeaturing walk-away convenience.

After a staining process is finished, theslide racks are transported via the transferstation from the stainer into the cover-slipper.

Leica TS5025Transfer station to automate the staining and coverslipping processbetween ST5020 (Multistainer) and CV5030

155



� Home �

The fully integrated Bond system is impro-ving the quality and workflow of IHC andISH staining. With complete automationand specially developed reagents (includingready-to-use antibodies), the Bond systemeliminates unnecessary manual handlingand ensures consistency at every step.

Bond also has a unique continuous proces-sing capability with three independent slidetrays. This allows laboratories to startslides as soon as they are ready whileretaining capacity to add more slides at anytime. Continuous processing ensuresstained slides are ready exactly when theyare needed.

Bond is a high-productivity system thatis ideal for laboratories pursuing LeanHistologyTM and six sigma principles.

Leica Bond™Fully automated IHC and ISH

156



� Home �

The Novocastra reagent range includesantibodies, probes, detection systems andancillaries. These advanced products reflectover 20 years of development experienceand provide superior results for immunohis-tochemistry and in situ hybridization.Manufactured in-house by Novocastrascientists, these products have earned areputation for quality and innovation. Ourhighly qualified scientific team collaboratesclosely with key opinion leaders. Thisensures our latest products focus ondiagnostically useful target proteins andare effective on formalin-fixed, paraffin-embedded human tissue. The range containsover 1,400 products and is continuallyexpanding with new clinically-focusedreagents.

Leica Novocastra™IHC and ISH reagents

157



� Home �158

� Home �

www.leica-microsystems.com/EM_Sample_Preparation

EM Sample PreparationEM Sample Preparation

http://www.leica-microsystems.com/EM_Sample_Preparation

� Home �

The Leica EM UC6 Ultramicrotome offers arange of outstanding features and benefitsof use from the absolute beginner to theskilled ultramicrotomist. The eucentric move-ment of the viewing system allows exami-nation of sections even with lowered waterlevel (as with e.g. Lowycryl) without loss ofergonomic posture. Defined positions provideoptimal positioning of the stereo micro-scope for alignment with glass and diamondknives. The innovative touch sensitivecontrol unit of the Leica EM UC6 enablesfast and safe alignment of knife and speci-men with help files and prompts to hand forbeginners. The AutoTrim function withprogrammable knife and cutting movementssimplifies the trimming process.

Leica EM UC6Ultramicrotome for ultrathin sectioning and facing

160

Ultramicrotomes

Range of use:� Industrial Materials� Biological Specimens

� Home �

The Leica EM FC6 cryochamber is designedfor low temperature sectioning of biologicaland industrial samples at temperaturesfrom -15 to -185°C. It sets new standards incryosectioning, not only for TEM but alsofor LM, SEM and AFM.

The Leica EM FC6 has been designed inaccordance with customer requests for amore ergonomic, easy-to-use instrumentwith a wider temperature range and in-creased sectioning stability. Ergonomichand rests ensure fatigue-free cryosectio-ning, while the integration of the EM FC6controls in the control unit of the LeicaEM UC6 saves space on the desk and theLED illumination of the chamber ensures aperfect view of the sections.

Leica EM FC6Low temperature sectioning system for Leica EM UC6

161

Ultramicrotomes


� Home �

Low temperature sectioning system forbiological and industrial samples at tempe-ratures as low as -185°C. The FCS fits ontothe Ultracut UCT, Ultracut R and Ultracut Sbecoming an integral part of the instrument.Sections can be made on either glass ordiamond knives, the knife holder accommo-dating both plus a trimming tool.Features include: contact free, through-the-wall, specimen holder system, open topdesign with no cover allowing easy accessand no disturbing ice-condensation.Warmed external surfaces for operatorcomfort. Memory for 3x3 settings of knife,specimen and gas temperature. Accuratecontrol of specimen, knife and chamber gastemperature. Alignment of knife withself-locking drives from outside thechamber.

Leica EM FCS Low temperature sectioning system for Leica Ultracut UCT

162

Ultramicrotomes


� Home �

The Leica EM AC20 is a new generation ofautomatic contrasting instruments for ultra-thin sections, developed in close coope-ration with the scientific community. Notonly does the Leica EM AC20 deliver highquality contrasting results but does so witha much lower reagent consumption. Theinstrument uses a peristaltic pump andnon-contact valves, which means thereagents travel through nothing but tubingon their way to the chamber where thegrids are located. This equals good resultsand easy maintenance.

Leica EM AC20Automatic contrasting instrument for electron microscopy

163

EM-Laboratory

Range of use:� Biological Specimens

� Home �

The new Leica EM AFS2 is capable of freezesubstitution and progressive lowering oftemperature (PLT) techniques as well asallowing low temperature embedding andpolymerization of resins. A stereomicro-scope for viewing and positioning of thesamples is available and the LED illumina-tion from within the chamber makes workeasy.

The freeze substitution processor EM FSPis an automatic reagent handling system.Sitting on the EM AFS2, it dispensesreagents for both freeze substitution andPLT techniques.

Leica EM AFS2Automatic freeze substitution system

164

EM-Laboratory


� Home �

With the unique Leica EM AMW, tissue canbe rapidly processed, embedded and poly-merized into resin for subsequent electronmicroscopy analysis with unmatchedautomation, in hours rather than days.Leica’s patented combination of microwavechamber and automatic reagent changerminimizes the manual effort and greatlyspeeds up processing time. Users can dedi-cate the time saved to other tasks andlaboratory workflow can be significantlystreamlined. The mono-mode microwavechamber of the Leica EM AMW focuses theenergy on a defined area, resulting in ahomogenous field pattern surrounding thesample, without hot and cold spots andnegating the need for water loads. A soft-ware-controlled, non-contact infraredsensor monitors the temperature duringprocessing and ensures accurate and re-producible results.

Leica EM AMWAutomatic microwave tissue processor for electron microscopy

165

EM-Laboratory


� Home �

The modular design of the Leica EM CPCcryoworkstation facilitates quick and easychange of the cryofixation modules allowingplunge freezing of tissue into propane, baregrid method with ethane or metal mirrorfreezing (slam freezing) onto gold coatedcopper blocks.

Leica EM CPC Universal cryofixation and cryopreparation system

166

EM-Laboratory


� Home �

The first automated immunogold labelingsystem, the innovative Leica EM IGL, sets anew industry standard by automating thetedious, time-consuming manual process ofimmunogold labeling. The Leica EM IGLoffers many advantages such as the simul-taneous labeling of 24 grids as well as theability to use up to 24 different primaryantibodies in one run.Automation with the EM IGL saves up to80% of the user's time, thus saves costsand frees technicians for more productiveactivities.Specimen safety is ensured by applying thecorrect sequence of reagents and minimi-zing cross contamination from forceps andloops during labeling. Reproducible resultscan be achieved time after time as theEM IGL follows the exact incubation timesspecified in the protocol.

Leica EM IGLThe first automated immunogold labeling system

167

EM-Laboratory


� Home �

The Leica EM KMR2 glass knifemakerproduces 45° glass knives from 6.4, 8 and 10mm thick glass utilizing the balanced-breakmethod. This method begins with a stan-dard 400 mm long glass strip in any of thethree thicknesses and continuously breaksthe strip into equal halves. You then have anumber of flat sided squares perfect formaking 45° knives suitable for light andelectron microscopy applications.Accessories include knife boxes, scoringwheels and trufs, the ultramicrotomist’sstandard boat for glass knives.

Leica EM KMR2Glass knifemaker

168

EM-Laboratory


� Home �

The Leica EM MM80 E Metal Mirror Cryofix-ation system consists of an LN2 cooled goldcoated, highly pure copper block used forthe impact freezing (slam freezing) of samplessuch as tissues and cell suspensions toform a vitreous ice layer (vitrification) forfreeze substitution and subsequent Trans-mission Electron Microscopy (TEM). Thisinstrument ensures high quality results withsimple operation, low initial costs, and lowoperating expense. In standby mode it usesless than 1 liter LN2/hour. The heating cycleautomatically switches off after use.

Leica EM MM80 E Metal mirror cryofixation system for impact freezing

169

EM-Laboratory


� Home �

EM-Laboratory

The Leica EM MP multiplate (hotplate)features three different temperatures formounting trufs onto glass knives, dryingsemithin sections on glass slides and stainingsemithin sections. A perfect tool for the specimen preparation laboratory.

Leica EM MP Multi-heating plate

170


� Home �

The new Leica EM PACT2 High PressureFreezer is the first step in the preparation ofsamples for many subsequent techniques,allowing high quality freezing up to 200 µminto the specimen.The instrument only needs to be connectedto a standard power supply and comes withits own compressor located on the sametrolley making it fully mobile.

The Rapid Transfer System EM RTS is anaccessory to Leica EM PACT2. It allows anease of sample preparation never beforeexperienced in the field of High PressureFreezing. Place your sample in the flatcarrier held in the fork and simply push itinto the instrument. The EM RTS does therest. It takes the sample, locks it into thepod and shoots it into the Leica EM PACT2where it is frozen – all in less than 2.5seconds!

Leica EM PACT2High pressure freezer

171

EM-Laboratory


� Home �

EM-Laboratory

The Leica EM RAPID milling device hasbeen designed for Research on ActivePharmaceutical Ingredient Dispersal prepa-ration for investigation with NIR spectro-photometry. With a tungsten carbide ordiamond miller, pills can be decapsulatedwithout any smearing caused by the pills’coating. A stereo microscope offers brilliantobservation while milling the sample. Theadjustable milling speed allows preparationof even fragile pills. Investigations on adefined layer can be performed by stepwiseremoval with pre-selected step advancesand total advance indication. Multiple glassslide and single pill holders reduce the leadtime to the NIR instrument. A low-noiseextraction unit with a Hepa filter provides asilent and safe environment.

Leica EM RAPID Pharmaceutical milling system for pills

172


� Home �

With the Leica EM TP tissues can be pro-cessed into resin for subsequent EM andLM analysis. The Tissue Processor has anexhaust system allowing safe use of toxicsubstances such as osmium. Individualseals reduce evaporation of reagents whilethe preheat/cool system allows the correctreagent temperature to be maintainedthroughout processing. Small vials areavailable for EM and larger ones for LMprocessing. The larger vials can also beused for high throughput EM-runs, utilizingthe Tripod system, where over 150 EMsamples can be processed in one run.

Leica EM TP Automated routine tissue processor

173

EM-Laboratory


� Home �

EM-Laboratory

The Leica EM TP4 C Automatic Tissue Pro-cessor, for resin processing of mineralizedtissue, has been adapted especially for theTechnovit 9100 new (Heraeus Kulzer) resinembedding system for fast, fully automatedprocessing at room temperature and at+4 °C.

Resin is the preferred embedding mediumfor undecalcified iliac crest biopsies aswell as specific organ biopsies such astesticular and renal, selected regions oflymph nodes, lymphomata and fine-needlebiopsies

Leica EM TP4 CAutomatic tissue processor for resin processing of mineralized tissue

174


� Home �

The Leica EM TRIM2 is a high speed millingsystem with an integrated stereo micro-scope and LED ring illuminator for trimmingof biological and industrial samples prior toultramicrotomy. A pivot arm and adjustmentassembly holds the specimen carrier foroptimum orientation. It can be used witheither tungsten carbide or diamond millingtools. The area of interest can be centeredand a flat block face milled onto the frontface of the sample.For TEM and LM the sample must betrimmed to shape by adjusting the angle ofthe pivot arm and trimming the desired blockshape – pyramidal, square rectangular, etc ...Viewing of the specimen perpendicular tothe axis of the stereomicroscope allowsdistance definition i.e. from the front face ofthe sample. All this takes place underconstant observation with the stereo micro-scope.

Leica EM TRIM2Specimen trimming device for TEM, SEM, LM

175

EM-Laboratory


� Home �

EM-Laboratory

The Leica EM TXP is a unique target pre-paration device especially developed forcutting and polishing samples prior to exami-nation by SEM, TEM and LM techniques. Itexcels with challenging specimens wherepin-pointing and preparing barely visibletargets becomes easy. Before the LeicaEM TXP, sawing, milling, grinding andpolishing exactly to the target was often avery time-consuming and difficult proce-dure as points of interest were easily missedand specimens often difficult to handle dueto their small size.

With the Leica EM TXP such samples caneasily be prepared.

Leica EM TXPTarget surfacing system

176

Range of use:� Industrial Materials

� Home �

www.leica-microsystems.com

Ord

er n

umbe

r: E

nglis

h 92

4107

� Home �

http://www.leica-microsystems.com

Leica Microsystems product catalog EN

Documents

Transcript of Leica Microsystems product catalog EN