Lecture 6 ELISA!. E L I S A nzyme- inked mmuno- orbent ssay Protein that facilitates a chemical...
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Transcript of Lecture 6 ELISA!. E L I S A nzyme- inked mmuno- orbent ssay Protein that facilitates a chemical...
![Page 1: Lecture 6 ELISA!. E L I S A nzyme- inked mmuno- orbent ssay Protein that facilitates a chemical reaction Immune system - antibodies Survey technique:](https://reader035.fdocuments.in/reader035/viewer/2022062722/56649f355503460f94c53bfe/html5/thumbnails/1.jpg)
Lecture 6
ELISA!
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ELISA
nzyme-
inked
mmuno-
orbent
ssay
Protein that facilitates a chemical reaction
Immune system - antibodies
Survey technique:• High throughput• Overview
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Antibodies?
VirusNH3
+
OH
O-
O
O
S
O
-O
O
+H3N
H-bonding signature
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Antibodies?
N
N
N
Cl
NH
NH
Atrazine
How can you use antibodies to detect it?
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Antibodies?
N
N
N
S
NH
NH
ProteinExpose
Collect sera
Harvest atrazine specific
antibodies
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How ELISA works
Competition for the antibody active sites between:
• Analyte of interest - Antigen (ie. Atrazine)• Analyte mimic or antigen mimic
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The Major Players
N
N
N
Cl
NH
NH
Compound of interest
Atrazine
=
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The Major PlayersCompetitor
N
N
N
Cl
NH
NH
Enzyme= E
ELISA
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The Major PlayersThe Antibody
ELISA
Fe
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Detection
H2N NH2 NHHNE
H2O2
Compound that is
detected
Blue Yellow
via enzyme catalyzed reaction
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Cross-reactivity
N
N
N
Cl
NH
NH
N
N
N
Cl
NH
NH
N
N
N
Cl
H2N NH
N
N
N
S
NH
NH
Atrazine Desethyl Atrazine
Simazine Ametryn
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