HPLC Systems. Column Chromatography HPLC Modes HPLC – System Components.
Lecture 12 HPLC
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Lecture 12 HPLC
description
Lecture 12 HPLC. Lecture 12 HPLC. GC setup. LC setup. LC Injector. Back to Van Deempter. A +. ∞. H . C . Back to Van Deempter. d p 2 . +. C e d p. ∞. H . . D MP. Mobile Phase. d p 2 . +. C e d p. ∞. H . . D MP. LC Columns Particle Size (d p ). d p 2 . +. - PowerPoint PPT Presentation
Transcript of Lecture 12 HPLC
![Page 1: Lecture 12 HPLC](https://reader033.fdocuments.in/reader033/viewer/2022061608/5681679b550346895ddcdc25/html5/thumbnails/1.jpg)
Lecture 12HPLC
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Lecture 12HPLC
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GC setup
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LC setup
![Page 5: Lecture 12 HPLC](https://reader033.fdocuments.in/reader033/viewer/2022061608/5681679b550346895ddcdc25/html5/thumbnails/5.jpg)
LC Injector
![Page 6: Lecture 12 HPLC](https://reader033.fdocuments.in/reader033/viewer/2022061608/5681679b550346895ddcdc25/html5/thumbnails/6.jpg)
Back to Van Deempter
H ∞ A + C
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H ∞ + dp
2DMP
Ce dp
Back to Van Deempter
![Page 8: Lecture 12 HPLC](https://reader033.fdocuments.in/reader033/viewer/2022061608/5681679b550346895ddcdc25/html5/thumbnails/8.jpg)
Mobile Phase
H ∞ + dp
2DMP
Ce dp
![Page 9: Lecture 12 HPLC](https://reader033.fdocuments.in/reader033/viewer/2022061608/5681679b550346895ddcdc25/html5/thumbnails/9.jpg)
LC ColumnsParticle Size (dp)
Dong, M. Today’s Chemist at Work. 2000, 9(2), 46-48.
H ∞ + dp
2DMP
Ce dp
![Page 10: Lecture 12 HPLC](https://reader033.fdocuments.in/reader033/viewer/2022061608/5681679b550346895ddcdc25/html5/thumbnails/10.jpg)
LC ColumnsParticle Size (dp)
H ∞ + dp
2DMP
Ce dp
![Page 11: Lecture 12 HPLC](https://reader033.fdocuments.in/reader033/viewer/2022061608/5681679b550346895ddcdc25/html5/thumbnails/11.jpg)
LC ColumnsTortuosity ()
Ascentis Express, Supelco, technical information
H ∞ + dp
2DMP
Ce dp
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Injector/column interaction
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Types of LC columns
C18 C8
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Column elution
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Size exclusion chromatography