Lab Equipment, Calibration and Maintenance and

Quality DataKSA 105/106/112KSA 207/208/210

MIS

SIO

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TATE

MEN

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Welcome to a rewarding career as a laboratory

professional!

You are making a difference in many people’s lives;

you are essential!

WH

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RE

01Mollie Wooden, Lab Supervisor at Monterey Bay Analytical Services: mwooden@mbasinc.com

David Holland, Lab Director at Monterey Bay Analytical Services: dholland@mbasinc.com

Mindy Boele, Water Quality Manager, City of Vacaville Utilities Department

02

03

David and Mindy will be monitoring the Q&A, if they can’t answer we will review before moving on to the next section.U

SE Q

&A

BO

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Questions during?

TOO

LSBefore you sign up for a test:

https://www.cwea.org/pdf/tcp/resources/hb_lab99.pdf

Study Guide:

G1: https://cert.cwea.org/wp-content/uploads/sites/3/2019/04/studyguide-lab1-ed2.pdf

G2: https://cert.cwea.org/wp-content/uploads/sites/3/2019/04/studyguide-lab2-ed2.pdf

Look online for references to things you don’t know or have access to in your

lab (YouTube!)

TOP

ICS

01 What to expect for the test – 5min

02

Lab Equipment (KSA 105/207)– 10min03

Calibration and Maintenance (KSA 106/208)- 20min04

Measuring Uncertainty/QA/QC (KSA112)- 20 min05

Essential Lab Duties (KSA210)- 10 min

FOC

US

• Computer based

• Multiple choice

• Choose best

available answer

• Feedback section at

the end of test

• No penalty for

wrong answers

KSA

(Knowledge

Skills and

Abilities)

WeightTotal covered

today

105 7%

106 8% 22%

112 7%

207 7%

208 9% 23%

210 7%

HO

W T

O S

TAR

T ST

UD

YIN

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Take the practice exam

• Write down KSAs failed

Read all sections of failed KSAs

• Focus on standard methods references

Retake test

• Reread KSA sections failed

• Take a class or ask for help

Right before test

• Write down all questions and correct answers

• Retake test until 100%

• Explain question and answer to someone else

Essential Lab Duties

KSA 210*Bonus section from your guys notes

to me ☺

HO

W T

O B

E U

SEFU

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Micro

Preparing media, autoclaving bio

hazards

Clean glassware

3 washes rule; soap or acid rinse?

Organize

Move/Dump old samples, label

bottles, create kits for sampling

Standardizing

Diluting stock acids and bases into workable range

Chemical Inventory

Create/Update inventory list

Preparing Solids

Prep filters, dishes, know how to properly

use a balance

Lab Equipment

KSA 105/207

**please write in Q&A box if there is something specific you would like me to cover

• Electronic and manual

• Quarterly Calibration

• Proper volume selection

PIP

ET A

ND

BU

RET

• Calibrate daily-bracketing weights used on balance

• SIG FIGS! How many decimal places are significant?

GR

AV

IMET

RIC

• Autoclace: Temperature, Pressure and Time (SM1090H)

• Bunsen burner= flame sterilization

• Oven at 160-180C (SM9020.4.g)

• Filter sterilize 0.2 um filter• UV light at 254-nm

STER

LIZI

NG

• Temperature <6°C - and holds steady

• Adequate space for samples

REF

RIG

ERA

TOR

S

• Check yearly• Compare

against NIST• Increments of

measurement• Min and max

temp BRACKET valueTH

ERM

OM

ETER

S

• Measures turbidity (SM2130)• Scattered light detectors at 90degree to

incident beam

NEP

HEL

OM

ETER

• Visual observation: PtCo method- Nessler tubes• Spec/Colorimeter: remove particulate matter

CO

LOR

IMET

RIC

• pH probe• SEC/EC probe

ELEC

TRO

MET

RIC

• LDO-luminescent dissolved oxygen

• ISE probe- ion selective, NH3

ELEC

TRO

MET

RIC

GRADE 2

• Used to measure anions: NO3, SO4, PO4, Cl, NO2, Br, F

ION

CH

RO

MA

TOG

RA

PH

SGRADE 2

• Digestion- addition of acid and heat: metals

• Extraction- acidify, shake/filter, draw off solvent: oil and grease• Liquid-liquid and

solid-phase• Filtration- 0.45 or 0.2 um• Distillation- heating until

steam and cooling distillate for analysis

SAM

PLE

PR

EPGRADE 2

1. Lauryl Sulfate Broth is sterilized before use for:

a. 15 minutes at 121°C (15 lbs. pressure).

b. 15 minutes at 118°C (12 lbs. pressure).

c. 20 minutes at 115°C (10 lbs. pressure).

d. 20 minutes at 121°C (15 lbs. pressure).

QU

IZ2. Standard Methods says

to incubate coliforms at 35.5C+/-0.5C, your thermometer must:

A. Read in 0.5 degree increments

B. Be electronicC. Be MercuryD. Read up to 35 degrees

3. What is the name of the vessel used to measure settleable solids?

a. Conical conesb. Volumetric flaskc. Beakerd. Imhoff Cones

QU

IZ

4. A pH measurement requires the following:a. a voltmeter, glass pH electrode, reference electrode, and a

temperature compensating device.b. a voltmeter, glass pH electrode, combination electrode,

and a temperature compensating device.c. a voltmeter, combination electrode, reference electrode

and a temperature compensating device.d. a voltmeter, glass pH electrode, reference electrode, and a

combination electrode.

QU

IZ

5. Which of the following parameters cannot be measured using a specific-ion electrode?

a. Nitrateb. Fluoridec. Chemical oxygen demandd. AmmoniaQ

UIZ

(G

2)

6. Preliminary treatment for acid-extractable metals would consist of:

a. filtering the sample, acidifying the filtrate, and analyzing directly.

b. filtering the sample, digesting the filter and residue, and performing analysis.

c. adding 5 mL of a 50% hydrochloric acid solution to a 10 mL sample, heating 15 minutes on a steam bath, filtering, and then diluting to 100 mL.

d. adding 5 mL of a 50% nitric acid solution to a 10 mL sample, heating until almost evaporated and nitrous oxide fumes develop, filtering, and then diluting to a 100 mL volume.

QU

IZ (

G2

)

Ask in Q&A box!

Questions?

Calibration and Maintenance

KSA 106/208

**please write in Q&A box if there is something specific you would like me to cover

MET

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EVEL

OP

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Working Range

Establish MDL and highest

value

Standards

Names, hazards and

concentrations

Units

Ppm, ppb, unitless, weird

units?

Frequency

How often do you calibrate?

Per lot, quarterly, daily?

**Usually est. in source method

Think about tests done using each instrument

TUR

BID

ITY

Working Range

Reporting limit: 0.05 NTU

-Usually dilute above 40 NTU

Standards

Formazin (usually 1.0 NTU), run room

temp to not fog glass

Units

NTU

Frequency

Quarterly

DO Working

Range

Looking for BODs in range of

7-9 mg/L

Standards

Glucose glutamic acid (GGA), CBOD needs nitrification inhibitor, you need Seed (influent or

purchased), dilution buffer,

sodium sulfite for DeCl2

Units

Mg/L

Frequency

Daily (with use)-prove probe is

working

pH Working

Range

1-14

Standards

pH standard and calibration

brackets potential results (NIST

traceable)*see next slide for

slope*

Units

mg/L

Frequency

Daily (with use)-prove probe is

working

pH changes greatly with temperature change

pH

https://www.mt.com/dam/SIPharmaEvent/How%20to%20Measure%20pH%20Correctly.pdf

• Titration volume appropriate

• pH probe calibrated• Titrator has no

bubbles, clear line• Always be aware of

what the color change or pH change will be

TITR

ATO

RS

SEC

/EC

Working Range

Measure of ions in water; good overall view of

WQ

Standards

KCl, cell constant (varies by probe),

temperature compensated

Units

Umhos/cm or uS/cm

(equivalent)

Frequency

Daily (with use)-prove probe is

working

• Light source, monochromator, detector; follows Beer’s law (abs. proportional to conc.)

• Applicable wavelengths available and stable

• Clean cuvettes, no scratches

• Air bubbles in samples• Correlation coefficient

(r^2): >0.995

SPEC

TRO

PH

OTO

MET

ERS

BA

LAN

CES

Working Range

Make sure you have enough sigfigs: TDS

xx.xxxx

Standards

NIST certified weights

Units

Mg or g- chose closest to

measured value

Frequency

Daily (with use)-prove probe is

working

• Monitored each time of use (daily) with appropriatethermometer

• Bacteria incubators checked twice daily 4 hours apart (weekends!)

OV

ENS,

INC

UB

ATO

RS,

FR

IDG

ES, W

ATE

RB

ATH

S

• Inspected monthly• UV at 254nm• Culture counter

has backlight• Cultures read 30-

300

MIC

RO

SCO

PES

, CU

LTU

RE

CO

UN

TER

, UV

LIG

HT

• Serial dilutions/step-wise: using too small of volumes increases error; must use multiple dilutions to achieve desired concentration

DIL

UTI

ON

1. At a given temperature, the intensity of the acidic or basic character of a solution is indicated by:

a. conductivity.b. pH or hydrogen ion

activity.c. specific ion probe

analysis.d. alkalinity.

QU

IZ2. A solution with a pH of 5

has a concentrationof hydrogen ions that is

how many times higherthan a solution with a pH of

7?a. 2 times higherb. 10-5 times higherc. 10-2 times higherd. 100 times higher

3. Conductance measurements are temperature compensated to:

a. 20.0°C.b. 25.0°C.c. 30.0°C.d. 20.0° to 25.0° C.

QU

IZ4. The conductivity meter is

calibrated with:a. 0.0100 N potassium

chloride standard.b. 0.0100 N silver chloride

standard.c. 0.0100 N sodium chloride

standard.d. 0.1000 N sodium chloride

standard.

Ask in Q&A box!

Questions?

Quality Lab Data

KSA 112

**please write in Q&A box if there is something specific you would like me to cover

• QA: say what you do

• QC: do what you say

• RECORD it all

QA

/QC

• You WILL be asked you to calculate mean median and mode of a set of numbers

• Note* RPD vs RSD- mean vs standard deviationMEA

N/S

TDD

EV, M

EDIA

N,

MO

DE

• Method Detection Limit, minimum reportable value with 99% confidence

• MDLs and MDLb• https://www.epa.gov/sites/production/files/2016-

12/documents/mdl-procedure_rev2_12-13-2016.pdf

MD

L

Let’s look at MBAS’s Alk

MDL Study…

• SM1020.13• Look at standard %Rec

or duplicate %RPD to gauge data quality

• Upper and lower:• Warning limit: PM

due• Control limit: reject

data

CO

NTR

OL

CH

AR

TS

• REGEANT• STANDARDS• WATER QUALITY• Manufacturer, lot, expiration, concentration,

expiration after diluted… • SM1080, proving quality of your reagent water

QU

ALI

TY O

F M

ATE

RIA

L

• REGEANT• STANDARDS• WATER QUALITY• Manufacturer, lot, expiration, concentration,

expiration after diluted… • SM1080, proving quality of your reagent water

QU

ALI

TY O

F M

ATE

RIA

L

• SM2020B.1 and .2• Initial demonstration of

capability: 4 LFBs, MDL, operational range

• Ongoing: continuing standard curves, LFBs, Method Blanks, QCS.

• Table 2020I and 2020 II show what QC is needed for each analysis

iDO

Cvs

oD

OC

Ask in Q&A box!

Questions?

Helpful trainings:https://events.cwea.org/events/?tribe_paged=1&tribe_

event_display=list&tribe_eventcategory=8https://www.events.rcac.org/rcac/Calendar.asp

MBAS Website: www.mbasinc.com

Instagram Lab Adventures: @CaLabAnalyst

Please fill out the survey at the end, I really look at it!See you next week for:

“Lab Calculations, Documentation and QA/QC”

**Bring your calculations you’re struggling with and we’ll go through them!

Thanks for attending!