Lab 1 Microscopy

Lab 1 Microscopy

• Parts & use of microscope

Lab 2 Cells & Tissues

• Cell theory• Cell structure, review pp. 17 & 18• Histology: epithelial, connective, muscular &

nervous• Slides– List on pp. 16 (plus frog skin)

Frog skin—simple squamous

http://www.google.com/imgres?imgurl=http://science.tjc.edu/Course/BIOLOGY/1413/frog%2520skin%2520high.jpg&imgrefurl=http://science.tjc.edu/Course/BIOLOGY/1413/Index.htm&usg=__4cL7Euwtmbemt1SpybRA-hBSMUI=&h=480&w=640&sz=90&hl=en&start=3&sig2=FvSAT1dq1SyYmrSzcLG4Mw&zoom=1&tbnid=62iQH6T1adK3UM:&tbnh=103&tbnw=137&ei=v_KeTvf2BaLm0QHQhujFCQ&prev=/search?q=frog+skin&hl=en&sa=X&rls=com.microsoft:en-US&rlz=1I7GGLD_enUS314&tbm=isch&prmd=ivns&itbs=1

Simple cuboidal

http://www.google.com/imgres?imgurl=http://www.carlalbert.edu/assets/images/Math%2520and%2520Science/simple_cuboidal_epithelium.jpg&imgrefurl=http://www.carlalbert.edu/index.php?id=496&usg=__2JBTmipm7U0Na-C-PwNMnaMbyBY=&h=1024&w=1280&sz=248&hl=en&start=3&sig2=ZoyNm3M0dYpSgej8kSGwEw&zoom=1&tbnid=MIyEzrs_Nlc4KM:&tbnh=120&tbnw=150&ei=GPOeTt_qN6b40gG60cSdCQ&prev=/search?q=simple+cuboidal&hl=en&sa=X&rls=com.microsoft:en-US&rlz=1I7GGLD_enUS314&tbm=isch&prmd=ivns&itbs=1

Simple columnar

http://www.google.com/imgres?imgurl=http://www.carlalbert.edu/assets/images/Math%2520and%2520Science/simple_columnar_epithelium.jpg&imgrefurl=http://www.carlalbert.edu/index.php?id=496&usg=__Y4px5AhJgqiAlZeYq2KtULETTMo=&h=1024&w=1280&sz=250&hl=en&start=1&sig2=FlX7gixT3bSSQv3Md7TNjQ&zoom=1&tbnid=tk_HOETTuHqM3M:&tbnh=120&tbnw=150&ei=Y_OeTo_vKcTa0QHr2LHWAg&prev=/search?q=simple+columnar&hl=en&sa=X&rls=com.microsoft:en-US&rlz=1I7GGLD_enUS314&tbm=isch&prmd=ivns&itbs=1

Human blood

http://www.google.com/imgres?imgurl=http://www.medicalhistology.us/twiki/pub/Main/ChapterTenSlides/a100_11_blood_smear_40x_wright_labeled.jpg&imgrefurl=http://www.medicalhistology.us/twiki/bin/view/Main/BloodAndBoneMarrowAtlas10&usg=__rvP52-zZUmKXw72JlEq73A7QZIs=&h=600&w=800&sz=481&hl=en&start=2&sig2=qkkHL7mExf0Grm5BFq_Zxw&zoom=1&tbnid=oHSNOG4SI4Bh3M:&tbnh=107&tbnw=143&ei=QvWeTvKYLYfY0QHQ1OiKCQ&prev=/search?q=blood+smear&hl=en&sa=X&rls=com.microsoft:en-US&rlz=1I7GGLD_enUS314&tbm=isch&prmd=ivns&itbs=1

Frog blood

http://www.google.com/imgres?imgurl=http://www.connecticutvalleybiological.com/images/JO510.jpg&imgrefurl=http://www.connecticutvalleybiological.com/rana-sp-frog-blood-smear-giemsa-p-14547.html&usg=__bE41R__bLjUrus8BjHRIo7sASSo=&h=488&w=650&sz=291&hl=en&start=9&sig2=L8_kkGgjyEPzuzjNJelG5A&zoom=1&tbnid=lPlsOJGWrY7F2M:&tbnh=103&tbnw=137&ei=cvWeTrnZNqLH0AHo3vzECQ&prev=/search?q=frog+smear&hl=en&sa=X&rls=com.microsoft:en-US&rlz=1I7GGLD_enUS314&tbm=isch&prmd=ivns&itbs=1

Loose areolar CT

http://www.google.com/imgres?imgurl=http://kentsimmons.uwinnipeg.ca/cm1504/15lab42006/loosehis.jpg&imgrefurl=http://kentsimmons.uwinnipeg.ca/cm1504/15lab42006/lb4pg6.htm&usg=__amHajYbRTsuOBXcusvxA6WA5eLw=&h=350&w=350&sz=138&hl=en&start=4&sig2=pnOHb832j93Awp1hNteIAA&zoom=1&tbnid=TtOS_AzCs_xiEM:&tbnh=120&tbnw=120&ei=mPOeTrenLsHk0QHR3pilCQ&prev=/search?q=areolar&hl=en&sa=X&rls=com.microsoft:en-US&rlz=1I7GGLD_enUS314&tbm=isch&prmd=ivns&itbs=1

Hyaline cartilage

http://www.google.com/imgres?imgurl=http://stevegallik.org/sites/histologyolm.stevegallik.org/images/hyalinecartilage.jpg&imgrefurl=http://histologyolm.stevegallik.org/node/123&usg=__YS1dJoB5vjHCmqzbg6l12eH3Ea8=&h=1024&w=1280&sz=215&hl=en&start=3&sig2=hukmEqns5U09XMhwKBSzVQ&zoom=1&tbnid=yTZmjikbMSG2gM:&tbnh=120&tbnw=150&ei=vPOeTp3CEoPc0QGBudXeDg&prev=/search?q=hyaline&hl=en&sa=X&rls=com.microsoft:en-US&rlz=1I7GGLD_enUS314&tbm=isch&prmd=ivns&itbs=1

Adipose

http://www.google.com/imgres?imgurl=http://images9.org/A-new/adipose-tissue/adipose-tissue-1.jpg&imgrefurl=http://images9.org/Images/a/adipose-tissue&usg=__JXGgNHIMfkVZ20d_OEKt6RUEWuQ=&h=1024&w=1280&sz=166&hl=en&start=3&sig2=KVKClGzx9W-1p3Osp5pf2Q&zoom=1&tbnid=fk12W1DZCm1ToM:&tbnh=120&tbnw=150&ei=5vSeTpqoOYfc0QHG7NmdCQ&prev=/search?q=adipose&hl=en&sa=X&rls=com.microsoft:en-US&rlz=1I7GGLD_enUS314&tbm=isch&prmd=ivns&itbs=1

bone

http://www.google.com/imgres?imgurl=http://www.cytochemistry.net/microanatomy/bone/bone1.jpg&imgrefurl=http://www.cytochemistry.net/microanatomy/bone/compact_bone_histology.htm&usg=__nxX-1HfhBLCDfzOCsHqjvSJnGHI=&h=491&w=722&sz=69&hl=en&start=1&sig2=9Xui9dRl0OtRY6UkVcB5ew&zoom=1&tbnid=KlVKBPr9zrtVFM:&tbnh=95&tbnw=140&ei=3POeTrKkLafu0gHrkdGWCQ&prev=/search?q=ground+bone&hl=en&sa=X&rls=com.microsoft:en-US&rlz=1I7GGLD_enUS314&tbm=isch&prmd=ivns&itbs=1

Smooth muscle

http://www.google.com/imgres?imgurl=http://clcpages.clcillinois.edu/home/bio567/pages/newtissues/Smooth%2520muscle%252001a.jpg&imgrefurl=http://clcpages.clcillinois.edu/home/bio567/pages/newtissues/smoothmuscle.htm&usg=__f9LGj3c_JzGW3vVCxRXXshxUIZk=&h=600&w=800&sz=67&hl=en&start=16&sig2=KHUdkR_I_9UR0ByuPKWhUw&zoom=1&tbnid=-KE8MIlHqiXm8M:&tbnh=107&tbnw=143&ei=L_SeTsL2Isb20gHVnrmjCQ&prev=/search?q=smooth+muscle&hl=en&sa=X&rls=com.microsoft:en-US&rlz=1I7GGLD_enUS314&tbm=isch&prmd=ivns&itbs=1

Skeletal muscle

http://www.google.com/imgres?imgurl=http://clcpages.clcillinois.edu/home/bio567/pages/newtissues/Skeletal%2520muscle%252003a.jpg&imgrefurl=http://clcpages.clcillinois.edu/home/bio567/pages/newtissues/skeletalmuscle.htm&usg=__QkfTZW7iJORpdc0ldTuZDqj0zdk=&h=600&w=800&sz=72&hl=en&start=4&sig2=cQHgGbD1pEDJ6BPiMPnrHg&zoom=1&tbnid=dNSqAnZ4sDwZkM:&tbnh=107&tbnw=143&ei=d_SeTsiICeTg0QG027WsCQ&prev=/search?q=skeletal+muscle&hl=en&sa=X&rls=com.microsoft:en-US&rlz=1I7GGLD_enUS314&tbm=isch&prmd=ivns&itbs=1

Cardiac muscle

Motor end plate

Lab 4 Development

• 5 phases• 3 germ layers• Cleavage: radial v. spiral• Development in sea star (slide) & frog (model)– Zygote, morula, blastula, gastrula, neurula (frog),

larva

• Development in chick (slides)• Extraembronic membranes (4)

Sea star development

Frog development

Lab 5 Morphology

• Symmetry• Body cavity• Metamerism• Cephalization• Protostomes v. Deuterostomes

Lab 6 Protozoa

• Single celled, eukaryotic, animal-like protists• Flagellated protozoa– Euglena (live & prepared)– Trypanosoma

• Amoeboid– Amoeba (live & prepared)– Foraminiferans (slide)– Radiolarians (slide)

Euglena

Amoeba

foraminiferans

radiolarians

Protozoa

• Alveolates– Paramecium caudatum

Lab 7 Porifera

• Sponges– Spicules of calcium carbonate or silica– Fibers of collagen or spongin– Outer pinacocytes– Inner choanocytes– Mesohyl in the middle

• Canal systems: asconoid, syconoid or leuconoid

Sponges

• Class Demospongiae: horny sponges• Class Calcispongiae: calcareous sponges• Class Hexactinellida: glass sponges

Class Demospongiae

Clathrina

Glass sponge

Phylum Cnidaria

• Radial symmetry• Diploblastic• Outer epidermis• Inner gastrodermis• Cnidocytes—nematocysts• Polyp & medusa stage

Cnidaria

• Class Hydrozoa– Obelia (prepared slide)– Hydra (live & prepared)– Physalia (preserved)

• Class Scyphozoa– Aurelia

• Class Anthozoa– Sea anemones & corals

Obelia

Physalia

Aurelia

Anthozoans

Lab 9 Platyhelminthes

• Flatworms• Triploblastic & bilateral• Class Turbellaria– Planaria (live & prepared slides)

• Class Trematoda– Complex life cycle—2+ hosts– Clonorchis sinensis—human liver fluke (slide)– Fasciola hepatica—sheep liver fluke (slide)– Cercaria—tadpole like stage (slide)

Planaria

Clonorchis sinensis

Fasciola hepatica

cercaria

Platyhelminthes

• Class Cestoda—tapeworms• Taenia piscivora (slide)• Scolex & proglottids

Lab 10 Pseudocoelomates

• Phylum Nematoda– Ascaris– Trichinella

Pseudocoelomates

• Phylum Rotifera– Corona & mastax

Lab 11 Phylum Mollusca

• Soft body• Mantle secretes shell• Triploblastic & coelomate• Radula

Class Polyplacophora

Class Bivalvia

Class Gastropods

Class Cephalopoda

Lab 12 Phylum Annelida

• Metamerism• setae

Class Polychaeta

Earthworm dissection

Class Oligochaeta

Class Hirudinea

Lab 13 Phylum Arthropoda

• Hardened exoskeleton• Jointed appendages• Molting

Subphylum Trilobita

Class MerostomataSubphylym Chelicerata

Subphylum Chelicerata

Subphylum MyriapodaClass DiplopodaClass Chilopoda

Class BranchiopodaSubphylum Crustacea

Class MaxillopodaSubphylum Crustacea

Class MalacostracaSubphylum Crustracea

Class InsectaSubphylum Hexapoda

Lab 1 Microscopy

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Transcript of Lab 1 Microscopy