“Microscopy and Cells” Lab Worksheet (M/C #2) NAME worksheet 6e.pdf“Microscopy and Cells”...
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“Microscopy and Cells” Lab Worksheet (M/C #2) NAME: Ex. 2-1: THE COMPOUND LIGHT MICROSCOPE Is your microscope monocular or binocular? What is the magnification of your ocular(s)? What is the magnification of each of your objectives? (list them in order of increasing magnification) Is your microscope equipped with phase-contrast optics? Does your microscope have a mechanical or stationary stage? Ex. 2-2: BASIC MICROSCOPE TECHNIQUES Move the slide slowly to the right. In what direction does the image in the ocular move? Is the image in the ocular inverted relative to the specimen on the stage? Is the working distance greater with the 40X or 10X objective? What is the total magnification with the 40X objective in place? What is the total magnification if the ocular is 20X and the objective is 100X? What is the diameter of the field of view with the 4X objective? 10X objective? 40X objective? What is the relationship between the size of the field of view and magnification? Focus on the region where the threads cross with the 4X objective. Are both threads in focus? Rotate the 10X objective into position and focus on the cross. Are both threads in focus? Which has a shorter depth of field, the 4X objective or the 10X objective? Which thread comes into focus first? Is this thread lying under or over the other thread? Which has a shorter depth of field, the 10X objective or the 40X objective? 53
Transcript of “Microscopy and Cells” Lab Worksheet (M/C #2) NAME worksheet 6e.pdf“Microscopy and Cells”...
“Microscopy and Cells” Lab Worksheet (M/C #2) NAME:
Ex. 2-1: THE COMPOUND LIGHT MICROSCOPE Is your microscope monocular or binocular?
What is the magnification of your ocular(s)?
What is the magnification of each of your objectives? (list them in order of increasing magnification)
Is your microscope equipped with phase-contrast optics?
Does your microscope have a mechanical or stationary stage?
Ex. 2-2: BASIC MICROSCOPE TECHNIQUES
Move the slide slowly to the right. In what direction does the image in the ocular move?
Is the image in the ocular inverted relative to the specimen on the stage?
Is the working distance greater with the 40X or 10X objective?
What is the total magnification with the 40X objective in place?
What is the total magnification if the ocular is 20X and the objective is 100X?
What is the diameter of the field of view with the 4X objective? 10X objective? 40X objective?
What is the relationship between the size of the field of view and magnification?
Focus on the region where the threads cross with the 4X objective. Are both threads in focus?
Rotate the 10X objective into position and focus on the cross. Are both threads in focus?
Which has a shorter depth of field, the 4X objective or the 10X objective?
Which thread comes into focus first? Is this thread lying under or over the other thread?
Which has a shorter depth of field, the 10X objective or the 40X objective?
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Ex. 2-3: THE STEREOSCOPIC MICROSCOPE
Which light gives you the best image of your object, reflected or transmitted light?
Sketch a sample of Elodea leaf at both low power and high power under the stereoscopic microscope:
____X total magnification (low power)
____X total magnification (high power)
Is it possible to see individual cells in the Elodea leaf using the stereoscopic microscope?
What about organelles?
Ex. 2-4: THE TRANSMISSION ELECTRON MICROSCOPE
Name three structures found in both light and electron microscopes.
What is the energy source used to view a specimen with an electron microscope?
What is the energy source used to view a specimen with a light microscope?
Describe how the magnification lenses differ for light vs electron of microscopes.
Ex. 2-5: THE ORGANIZATION OF CELLS
Sketch the following as viewed under the compound microscope, identifying any structures you can:
AMOEBA ____X total magnification
TRICHONYMPHA ____X total magnification
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SCENEDESMUS ____X total magnification
VOLVOX ____X total magnification
ELODEA ____X total magnification
HUMAN EPITHELIAL CELLS ____X total magnification
List the structures in Elodea that can be seen with the compound light microscope.
REVIEWING YOUR KNOWLEDGE 1. What characteristics do all eukaryotic cells have in common? 2a. Indicate the cellular structures that are found in only plant or animal cells. 2b. Describe the biological role of each structure unique to plant cells. 3. Review the criteria used to distinguish between colonial and multicellular organisms. Why is Volvox now considered multicellular?
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