IUB FCCF – FACSAria II Questionnaire · PDF file 2019. 11. 7. · IUB FCCF...

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Transcript of IUB FCCF – FACSAria II Questionnaire · PDF file 2019. 11. 7. · IUB FCCF...

  • IUB FCCF – FACSAria II Questionnaire

    Sort form rev. June 2015

    Please provide ALL the information requested below: Name: _____________________________ Lab affiliation: ___________________ Account #: __________ Sample information Biosafety Level 1 ○ 2 ○ Sample is: mouse ○ human ○ other ○ : if other, name of organism is :______________________ Sample type: primary ○ tissue culture ○ other ○ : if other, sample type is:____________________ Cell/particle name(s) (list ALL cells or particles in the sample, even if not being sorted): For the following questions, if the answer is no, please type N/A (Not Applicable) in the box Sample is/contains a biohazard: No ○ Yes ○ Name of biohazard: ______________________________ Sample contains a viral vector: No ○ Yes ○ Name of viral vector: ______________________________ Transfection Date (Month/Day/Year): __________ Sample is genetically modified: No ○ Yes ○ Genetic modification: _____________________________ Sample contains: HBV: No ○ Yes ○ HCV: No ○ Yes ○ HIV: No ○ Yes ○ TB: No ○ Yes ○ Sample contains a disease carrying organism: No ○ Yes ○ Name of organism: __________________

    See next page for Aria II set-up questionnaire

  • IUB FCCF – FACSAria II Questionnaire

    Sort form rev. June 2015

    Please provide ALL the information requested below: Aria II set-up information Type of Aria II session requested: Sort ○ Analysis ○ Both ○ Nozzle: 70µm 85µm 100µm 130µm Cell or particle diameter (used to choose nozzle size and sort set-up): _______________ micron If multiple cell types are being analyzed or sorted, please list all cell/particle types and diameters here: Total number of samples (including controls): _________ Number of samples for sorting: ___________ Sample volume (controls): ___________ ml(s) Sample volume (sample): ____________ ml(s) Cell concentration (controls): ____________/ml Cell concentration (sample): ____________/ml Collection device #1 Collection device #2 Collection device #3 If using a multi-well plate, If using a multi-well plate, If using a multi-well plate, how many wells: how many wells: how many wells: Total number of collection tubes: Total number of collection plates: Number of cells to collect: ___________ per Sample acquisition temperature: Room temperature ○ 4C ○ Sample collection temperature: Room temperature ○ 4C ○ List all of the stains, fluorophores, antibody-fluorophore combinations, etc. (i.e. PI, GFP, APC): List any fixatives, chemicals, etc. that have been added to the sample: Any additional information:

    Name: Lab affiliation: Account: Name of biohazard: Name of viral vector: Genetic modification: Name of organism: Total number of samples including controls: Number of samples for sorting: Sample volume controls: Sample volume sample: Cell concentration controls: Cell concentration sample: Number of cells to collect: Biosafety: Off Sample is: Off Cell/particle names: Biohazard: Off Transfection Date: Genetically modified: Off Viral vector: Off HBV: Off TB: Off HCV: Off HIV: Off Diameter: name of organism: sample type: Disease carrier: Off Nozzle: Off Cell types and diameter: Collection device 1: [select one] Collection device 2: [select one] Wells 1: [n/a] Wells 2: [n/a] Wells 3: [n/a] Collection device 3: [select one] Number collection tubes: Number collection plates: collect: [select one] Sample temp 1: Off Sample temp 2: Off Additional info: Fluorophores: Fixatives, Chemicals: Sample type buttons: Off Aria II session buttons: Off