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है”ह”ह

IS 2923 (1995): Carmoisine, Food Grade [FAD 8: FoodAdditives]

IS 2923 : 1995

Yn

IYltlll ?

( T mdian Standard

CARMOISINE, FOOD GRADE - SPECIFICATION

( Second Revision ) l

UDC 664099.62 : 667.283.8 CAR

0 BIS 1995

BUREAU OF INDIAN STANDARDS MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG

NEW DELHI 110002

March 1995 Price Group 2

xxxx2008

Food Additives Sectional Committee, FAD 8

FOREWORD

This Indian Standard (Second Revision) was adopted by the Bureau of Indian Standards, after the draft finalized by the Food Additives Sectional Committee had been approved by the Food and Agriculture Division Council.

This standard is one of the series of Indian Standards on synthetic food colours permitted under the Prevention OfFoodAdulteration Rules, 1955 issued by the Ministry of Health, Government of India. These rules, inter-alia prescribe :

‘All food colours including natural colouring matter and permitted synthetic food colours and their preparations or mixtures excluding saffron and curcumin shall be sold only under the BIS Certification Mark’.

This Indian Standard was first issued in 1964. It was revised in 1974 to bring it in line with the FAO/WHO specifications after taking into account the indigenous data generated. This standard is being revised again taking into consideration the latest FAOWHO specifications for food colours [see FAO Food and Nutrition Paper 31/l (1984) ‘Specification for identity and purity of food colours’], latest specifications laid down under Food Chemical Codex, EEC Directives, Canadian Food Laws and Food and Drugs Act of USA In addition, due consideration has been given to indigenous data.

In this revision the minimum requirement for total dye content has been raised and the maximum requirement of loss on drying at 135°C and chloride and sulphate expressed as sodium salt has been decreased. The limits for water insoluble matter and subsidiary dyes have been made more stringent to align them with the International requirements.

While formulating this standard, necessary consideration has been given to the relevant Rules prescribed by the Government of India under the Prevention of Food Adulteration Act, 1955 and the Standards of Weights and Measures (Packaged Commodities) Rules, 1977.

Description

Common IVame - Carmoisine.

Synonyms - Azorubine, Cl. Food Red 3, E.E.C. Serial No. E 122.

Colour of the 0.1 Percent (mlv) Solution in Distilled Water -red.

Colour Index Number (1975) - No. 14720.

Class-Monoazo.

Chemical Name-Disodium salt of 2-(4-sulpho-l-naphthylazo)-l-naphthol-4-sulphonic acid.

Empirical Formula-CzoH1zN207S2Na2.

Molecular Weight-502.44.

Sofubifify -- Soluble in water. Sparingly soluble in ethanol.

For the purpose of deciding whether a particular requirement of this standard is complied with, the final value, observed or calculated, expressing the result ofa lest or analysis, shall be rounded off in accordance with IS 2 : 1960 ‘Rules for rounding off numerical values (revised)‘. The number of significant places retained in the rounded off value should be the same as that of the specified value in this standard.

Indian Standard

CARMOISINE, FOOD GRADE - SPECIFICATION

( Second Revision ) 1 SCOPE 3.2 Freedom from Contaminants,

1.1 This standard prescribes the requirements and the methods of sampling and test for carmoisine,

. food grade.

2 REFERENCES

The following Indian Standards are necessary ad- juncts to this standard:

IS No. Title

1070 : 1992 Reagent grade water (third revision)

1699 : 1994 Methods of sampling and test for food colours (second revision)

2491 : 1972 Code for hygienic conditions for food processing units @sst revision)

3 REQUIREMENTS

3.1 The material shall conform to the require- ments prescribed in Table 1.

Precautions shall be taken to ensure that the material is free from mercury, selenium and chromium in any form; aromatic amines, aromatic nitro compounds, aromatic hydrocarbons, and cyanides.

3.3 The product shall be processed, packed, stored and distributed under hygienic conditions in licenced premises (see IS 2491 : 1972).

4 PACKING AND MARKING

4.1 Packing

The material shall be packed in glass containers, metal containers, polyethylene containers, or cardboard containers suitably lined with polyethylene. Subject to agreement between the purchaser and the vendor any other suitable con- tainer may also be used.

Table 1 Requirements for Carmoisine K&use 3.1)

SI NO.

(1)

0

Charackristk

(2)

Total dye content, corrected for sample dried at 105 2 1°C for 2 h, percent by mass, Min

ii) Loss on drying at 135”C, percent by mass, Mm and

Chloride and sulphates expressed as sodium salt, percent by mass, Mar .

iii)

iv)

v)

vi)

Water-insoluble matter, percent by mass,Mor

‘Combined ether atrkts, percent by mass, MP*

Subsidiary dyes, percent by mass, Mar

Dye intermediate, percent by mass, Mar

vii)

viii)

iX)

Lead, mgkg. Mau

Arsenic, mgkg, Mar

Heavy metals, mgkg, Mar

l&quirewal

(3)

87

13

0.2

0.2

1.0

0.5

10

3

40

Method of Test, R&lo Annex of claame of

tht standard IS 1699 : 1994 (4) (9

A

C (Routine method)

6

13 14

7

8

(Ref2na method)

15

15

16

1

IS 2923 : 1995

4.2 Marking

4.2.1 Each container shall be legibly and indelibly marked with the following information :

a) b) Cl d) e)

9 g) h) i) ‘0

The word’s ‘FOOD COLOUR’; Common name of the colour; c:lemical name of the colour; Colour index number; Names of the major dye intermediates present; Source of manufacture; Date of manufacture; Net mass in grams or kilograms; Batch or code number; and Any other requirements as specified under the Standards of Weights and Measures (Packaged Commodities) Rules, 19771 Prevention of Food Adulteration Rules, 1955.

4.2.2 BIS Certification Marking Unless specified otherwise, pure chemicals and dis- The product may also.be marked with the Standard tilled water (see IS 1070 : 1992) shall be employed

Mark. in tests.

4.2.2.1 The use of the Standard Mark is governed by the provisions of the Bureau of Indian Standards

NOTE - ‘Pure chemicals’ shall mean chemicals that do not contain impurities which affect the results of analysis.

Act, 1986 and the Rules and Regulations made thereunder. The details of conditions under which the licence for the use of Standard Mark may be granted to manufacturers or producers may be obtained from the Bureau of Indian Standards.

5 SAMPLING

5.1 Representative samples of the material for tests shall be drawn and criteria for ascertaining conformity to the requirements of this standard shall be determined according to the method prescribed in 4 of IS 1699 : 1994.

6TESTS

6.1 Tests shall be carried out as prescribed in co1 4 and 5 of Table 1.

6.2 Quality of Reagents

ANNEX A

[ Table 1, SZ No. (i) ]

DETERMINATION OF TOTAL DYE CONTENT

A-0 GENERAL 105 + 1°C for 2 hours. Calculate the loss of mass on

‘Iwo methods, spectrophotometric method and the drying; from this data, calculate the dry mass of the

titanium trichloride method, are specified. In case sample (M) in the final solution taken for measure-

of dispute, spectrophotometric method shall be ment Of OPtica1 densit~* regarded as the reference method. A-l.3 Calculation A-l SPECTROPHOTOMETRIC METHOD

A-l.1 Appamtus

Suitable spectrophotometer with properly calibrated scales for both wave length and optical density. However, a suitable spectrocolorimeter may also be used after calibration against a spectrophotometer.

A-1.2 Procedure

Weigh accurately about 250 mg of the dye sample and dissolve with 0.1 N hydrochloric acid in a 250

Total dye content in the

sample, percent = ODxlOo M x 520

where

OD = optical density found;

M = dry mass of the sample, in g, in 100 ml solution; and

520 = E’% I cm, 516 nm, for carmoisine in 0.1 N hydrochloric acid.

ml volumetric flask, dilute this with the same sol- A-2 TITANIUM TRICHLORIDE METHOD vent to make a final concentration of 1 mg/lOO ml (approximately). Findout theoptical densifyofthis A-2.1 Procedure dilute solution, against 0.1 N hydrochloric acid as blank, at 516 nm, in a glass cell with 10.0 mm light ‘The method given in 5 of IS 1699 : 1994 shall be

path. followed. The percentage of total dye content shall

A-1.2.1 Simultaneously weigh accurately about 2 g be calculated using the following equation:

of the dye-sample and dry this in an air-oven at 1 ml of 0.1 N Tic13 = 0.012 56 g of carmoisine

2

IS 2923 : 1995

ANNEX B

[7izble 1, Sl No. (v)]

DETERMINATION OF SUBSIDIARY DYES

B-l The method given in 9 of IS 1699 : 1994 using the following conditions shall be followed:

Developing solvent No. 4 Height of ascent of solvent front : approximately 17 cm.

ANNEX C

[Table 1, Sl No. (vi)]

DETERMINATION OF DYE INTERMEDIATES

C-l APPARATUS C-3.2.3 Reference Substances

C-l.1 Chromatography Tank and Ancillary Equip ment - as given under 93.1 of IS 1699 : 1994.

C-l.2 Micrusyrlnge - capable of delivering 0.2 ml with a tolerance of + 0.002 ml.

C-l.3 Ultraviolet Lamp - at 365.5 nm.

C-l.4 Filter Paper - Whatman No. 1 or equivalent.

C-2 REAGENTS

C-2.1 Ammonium Hydroxide Solution - sp. gr. 0.923.

C-2.2 1-Naphthylamine-4-Sulphonic Acid

C-2.3 2-Naphtho13 : 6 Disulphonic Acid

C-2.4 Developing Solvent - sodium bicarbonate solution (10 percent m/v).

C-3 PROCEDURE

a) 1-naphthylamine-4-sulphonic acid solu- tion [ C-3.1.2 (a)] - 5 ~1 shall be equivalent to 0.5 p,g corresponding to 0.25 percent in the dye sample. 10 ~1 shall be equivalent to 1 pg cor- responding to 0.5 percent in the dye sample.

b) 1-naphthol-4-sulphonic acid solution [C-3.1.2(b)] - 5 ~1 shall be equivalent to 0.5pg corresponding to 0.25 percent in the dye sample. 10 ~1 shall be equivalent to 1 c(g cor- responding to 0.5 percent in the dye sample.

C-3.1 Preparation of Solutions

C-3.1.1 Prepare 2 percent m/v solution of dye in a mixture of nine parts of water and one part of the ammonium hydroxide.

C-3.1.2 Reference Substances

a) PrepareO.O1 percent, on 100 percent basis, m/v solution of 1-naphthylamine-4-sul- phonic acid solution in nine parts of water and one part of ammonium hydroxide.

b) Prepare 0.01 percent, on 100 percent basis, m/v solution of 1-naphthol-4-sulphonic acid in a mixture of nine parts of water and one part of ammonium hydroxide.

C-3.2 Test Substances

C-3.3 Mark out a sheet of chromatographic paper as shown in Fig. 1 of IS 1699 : 1994 and apply 10 ~1 of the dye solution as uniformly as possible with the help of microsyringe. Also apply reference substances (C-3.1.2). Mount the sheet together with a plain sheet to act as a blank in the frame (C). Pour sufficient developing solvent into the tray (0) to bring the surface of the solvent about one cen- timltre below the base line of the chromatogram sheet. Put the frame (C) in the same position and replace the cover. Take out the paper after 30 cm run. Dry in an oven at 70 to 75 “C.

C-3.4 Detection and Semi-quantitative Estima- tion

C-3.2.1 L$? Solution

10 ~‘1 of the solution (C-3.1.1) shall be equivalent to 200 g of the sample.

Inspect the chromatogram under ultraviolet light. Compare visually the intensity of the fluorescence of the sample with the reference substances and report thecontent of I-naphthylainine-4-sulphonic acid, and 1-naphthol-4-sulphonic acid which shall be nearest in intensity to one of the corresponding reference substances.

Bureau of Indian Standards

BIS is a statutory institution established under the Bureau ofZndian Standards Act, 1986 to promote harmonious development of the activities of standardization, marking and quality certification of goods and attending to connected matters in the country.

Copyright

BIS has the copyright of all its publications. No part of these publications may be reproduced in any form without the prior permission in writing of BIS. This does not preclude the free use, in the course of implementing the standard, of necessary details, such as symbols and sizes, type or grade designations. Enquiries relating to copyright be addressed to the Director (Publications), BIS.

Review of Indian Standards

Amendments are issued to standards as the need arises on the basis of comments. Standards are also reviewed periodically; a standard along with amendments is reaffirmed when such review indicates that no changes are needed; if the review indicates that changes are needed, it is taken up for revision. Users of Indian Standards should ascertain that they are in possession of the latest amendments or edition by referring to the latest issue of ‘BIS Handbook’ and ‘Standards Monthly Additions’.

This Indian Standard has been developed from Dot : No. FAD 8 ( 258 ).

Amendments Issued Since Publication

Amend No. Date of Issue Text Affected

,

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