Introduction to Microscopy:
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Transcript of Introduction to Microscopy:
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Introduction to Microscopy:
• The Light Microscope
• The TEM
• The SEM
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Introduction to Microscopy (Use of Microscopes to study minute [small] objects)
• The image to the right is a light microscopelight microscope
• Light microscopes bounce light waves off of an object. The light source may be from the sun or from a bulb from a lamp.
• The diaphragm of a light microscope controls the amount of light the enters the stage to illuminate the specimen being examined.
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Resolution: The resolution of a microscope is a measure of the clarity the image produced by the scope; the smallest distance between
to points that can be seen as being separate.
• http://www.olympusmicro.com/primer/java/microscopy/airydiscs/index.html
• The image below shows the difference between the resolution of a light microscope versus an electron microscope
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Electron Microscopes: these microscopes bounce a beam of electrons off of an object instead of light. These
microscopes have a high resolution (smaller distance between two points)
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There are two types of electron microscopes:
• SEM (scanning electron microscope)– Produces a three-dimensional image)
• TEM (transmission electron microscope)– Produces a two-dimensional image)
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SEM Images (Three-Dimensional)
http://micro.magnet.fsu.edu/primer/java/electronmicroscopy/magnify1/index.html
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TEM images
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Which has better resolution of image?
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Controls amount of light entering the stage
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