Introduction microscopy

18
Introduction microscopy Introduction microscopy most cells are .01-.1 mm unaided eye sees .1mm Hooke built microscope (30X)

description

Introduction 􀁡microscopy. Introduction 􀁡microscopy 􀁡most cells are .01-.1 mm 􀁡unaided eye sees .1mm 􀁡Hooke 􀁠built microscope (30X). Purpose of Microscope. 􀁡magnification 􀁠 enlarged image by a factor 􀁡resolving power 􀁠 ability to separate two adjacent points in a specimen - PowerPoint PPT Presentation

Transcript of Introduction microscopy

Page 1: Introduction microscopy

Introduction1048673microscopy

Introduction1048673microscopy

1048673most cells are 01-1 mm1048673unaided eye sees 1mm

1048673Hooke1048673built microscope (30X)

Purpose of Microscope

1048673magnification1048673 enlarged image by a factor

1048673resolving power1048673 ability to separate two adjacent points in a

specimen1048673contrast

1048673 how well details stand out against thebackground1048673parfocal

1048673remain in focus when switching objectives

Rules and Care of theMicroscope abridged

1048673carry microscope correctly1048673clean lenses with lens paper and slides

with Kimwipes1048673set smallest objective in place (4X) and

bring stage close of objectives1048673use oil with oil immersion lens (100X) only

1048673clean all lenses when finished1048673have instructor check scope before storing

bull The Compound Microscopebull Metric measurement of a field of viewbull 1048673ocular = 10Xbull 1048673mag of lens total mag (ocular x obj)bull 4X 40Xbull 10X 100Xbull 40X 400Xbull 100X 1000X

bull 6bull Exercisesbull Ex 71 fill in tablebull Ex 72 depth of focus bull colored threadsdetermine orderbull Ex 73 image orientationbull letter ldquoerdquo draw at 40x and 100xbull 1048673Ex 74 dissecting microscopebull 1048673Observe bacterial culture dish

Other Microscopes

bull 1048673dissectinggreater working distancereflected and transmitted light

bull 1048673light microscope

bull 1048673phase contrast microscope

bull 1048673transmission electron microscope TEM

bull 1048673scanning electron microscope SEM

Cell Theory

bull Cell Theory

bull 1048673all organisms are made of cells

bull 1048673the cell is the smallest living thing

bull 1048673all cells arise from pre-existing cells

Eukaryotic Cells

bull Plant Elodea cells w water

bull draw and label 400X total mag

bull Label nucleus plasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Chloroplastvacuole

Eukaryotic Cells

bull Plant Onion bulb cellsw iodine

bull 1048673draw and label 400X total mag

bull Label nucleus w DNAplasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Nucleolusvacuole

Eukaryotic Cells

bull Animal human cheek cells

bull w methylene blue stain

bull draw and label 400X total mag

bull nucleus w DNA plasma membranecytoplasm w cytosol

Prokaryotic Cells

bull 1048673label cellcell wall of peptidoglycan

bull plasma membranecytoplasm w cytosol

bull Ribosomenucleoid w DNA

bull draw three basic shapes

bull determine stain reaction

bull Gram positive = purple

bull Gram negative = red

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 2: Introduction microscopy

Purpose of Microscope

1048673magnification1048673 enlarged image by a factor

1048673resolving power1048673 ability to separate two adjacent points in a

specimen1048673contrast

1048673 how well details stand out against thebackground1048673parfocal

1048673remain in focus when switching objectives

Rules and Care of theMicroscope abridged

1048673carry microscope correctly1048673clean lenses with lens paper and slides

with Kimwipes1048673set smallest objective in place (4X) and

bring stage close of objectives1048673use oil with oil immersion lens (100X) only

1048673clean all lenses when finished1048673have instructor check scope before storing

bull The Compound Microscopebull Metric measurement of a field of viewbull 1048673ocular = 10Xbull 1048673mag of lens total mag (ocular x obj)bull 4X 40Xbull 10X 100Xbull 40X 400Xbull 100X 1000X

bull 6bull Exercisesbull Ex 71 fill in tablebull Ex 72 depth of focus bull colored threadsdetermine orderbull Ex 73 image orientationbull letter ldquoerdquo draw at 40x and 100xbull 1048673Ex 74 dissecting microscopebull 1048673Observe bacterial culture dish

Other Microscopes

bull 1048673dissectinggreater working distancereflected and transmitted light

bull 1048673light microscope

bull 1048673phase contrast microscope

bull 1048673transmission electron microscope TEM

bull 1048673scanning electron microscope SEM

Cell Theory

bull Cell Theory

bull 1048673all organisms are made of cells

bull 1048673the cell is the smallest living thing

bull 1048673all cells arise from pre-existing cells

Eukaryotic Cells

bull Plant Elodea cells w water

bull draw and label 400X total mag

bull Label nucleus plasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Chloroplastvacuole

Eukaryotic Cells

bull Plant Onion bulb cellsw iodine

bull 1048673draw and label 400X total mag

bull Label nucleus w DNAplasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Nucleolusvacuole

Eukaryotic Cells

bull Animal human cheek cells

bull w methylene blue stain

bull draw and label 400X total mag

bull nucleus w DNA plasma membranecytoplasm w cytosol

Prokaryotic Cells

bull 1048673label cellcell wall of peptidoglycan

bull plasma membranecytoplasm w cytosol

bull Ribosomenucleoid w DNA

bull draw three basic shapes

bull determine stain reaction

bull Gram positive = purple

bull Gram negative = red

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 3: Introduction microscopy

Rules and Care of theMicroscope abridged

1048673carry microscope correctly1048673clean lenses with lens paper and slides

with Kimwipes1048673set smallest objective in place (4X) and

bring stage close of objectives1048673use oil with oil immersion lens (100X) only

1048673clean all lenses when finished1048673have instructor check scope before storing

bull The Compound Microscopebull Metric measurement of a field of viewbull 1048673ocular = 10Xbull 1048673mag of lens total mag (ocular x obj)bull 4X 40Xbull 10X 100Xbull 40X 400Xbull 100X 1000X

bull 6bull Exercisesbull Ex 71 fill in tablebull Ex 72 depth of focus bull colored threadsdetermine orderbull Ex 73 image orientationbull letter ldquoerdquo draw at 40x and 100xbull 1048673Ex 74 dissecting microscopebull 1048673Observe bacterial culture dish

Other Microscopes

bull 1048673dissectinggreater working distancereflected and transmitted light

bull 1048673light microscope

bull 1048673phase contrast microscope

bull 1048673transmission electron microscope TEM

bull 1048673scanning electron microscope SEM

Cell Theory

bull Cell Theory

bull 1048673all organisms are made of cells

bull 1048673the cell is the smallest living thing

bull 1048673all cells arise from pre-existing cells

Eukaryotic Cells

bull Plant Elodea cells w water

bull draw and label 400X total mag

bull Label nucleus plasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Chloroplastvacuole

Eukaryotic Cells

bull Plant Onion bulb cellsw iodine

bull 1048673draw and label 400X total mag

bull Label nucleus w DNAplasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Nucleolusvacuole

Eukaryotic Cells

bull Animal human cheek cells

bull w methylene blue stain

bull draw and label 400X total mag

bull nucleus w DNA plasma membranecytoplasm w cytosol

Prokaryotic Cells

bull 1048673label cellcell wall of peptidoglycan

bull plasma membranecytoplasm w cytosol

bull Ribosomenucleoid w DNA

bull draw three basic shapes

bull determine stain reaction

bull Gram positive = purple

bull Gram negative = red

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 4: Introduction microscopy

bull The Compound Microscopebull Metric measurement of a field of viewbull 1048673ocular = 10Xbull 1048673mag of lens total mag (ocular x obj)bull 4X 40Xbull 10X 100Xbull 40X 400Xbull 100X 1000X

bull 6bull Exercisesbull Ex 71 fill in tablebull Ex 72 depth of focus bull colored threadsdetermine orderbull Ex 73 image orientationbull letter ldquoerdquo draw at 40x and 100xbull 1048673Ex 74 dissecting microscopebull 1048673Observe bacterial culture dish

Other Microscopes

bull 1048673dissectinggreater working distancereflected and transmitted light

bull 1048673light microscope

bull 1048673phase contrast microscope

bull 1048673transmission electron microscope TEM

bull 1048673scanning electron microscope SEM

Cell Theory

bull Cell Theory

bull 1048673all organisms are made of cells

bull 1048673the cell is the smallest living thing

bull 1048673all cells arise from pre-existing cells

Eukaryotic Cells

bull Plant Elodea cells w water

bull draw and label 400X total mag

bull Label nucleus plasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Chloroplastvacuole

Eukaryotic Cells

bull Plant Onion bulb cellsw iodine

bull 1048673draw and label 400X total mag

bull Label nucleus w DNAplasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Nucleolusvacuole

Eukaryotic Cells

bull Animal human cheek cells

bull w methylene blue stain

bull draw and label 400X total mag

bull nucleus w DNA plasma membranecytoplasm w cytosol

Prokaryotic Cells

bull 1048673label cellcell wall of peptidoglycan

bull plasma membranecytoplasm w cytosol

bull Ribosomenucleoid w DNA

bull draw three basic shapes

bull determine stain reaction

bull Gram positive = purple

bull Gram negative = red

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 5: Introduction microscopy

bull 6bull Exercisesbull Ex 71 fill in tablebull Ex 72 depth of focus bull colored threadsdetermine orderbull Ex 73 image orientationbull letter ldquoerdquo draw at 40x and 100xbull 1048673Ex 74 dissecting microscopebull 1048673Observe bacterial culture dish

Other Microscopes

bull 1048673dissectinggreater working distancereflected and transmitted light

bull 1048673light microscope

bull 1048673phase contrast microscope

bull 1048673transmission electron microscope TEM

bull 1048673scanning electron microscope SEM

Cell Theory

bull Cell Theory

bull 1048673all organisms are made of cells

bull 1048673the cell is the smallest living thing

bull 1048673all cells arise from pre-existing cells

Eukaryotic Cells

bull Plant Elodea cells w water

bull draw and label 400X total mag

bull Label nucleus plasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Chloroplastvacuole

Eukaryotic Cells

bull Plant Onion bulb cellsw iodine

bull 1048673draw and label 400X total mag

bull Label nucleus w DNAplasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Nucleolusvacuole

Eukaryotic Cells

bull Animal human cheek cells

bull w methylene blue stain

bull draw and label 400X total mag

bull nucleus w DNA plasma membranecytoplasm w cytosol

Prokaryotic Cells

bull 1048673label cellcell wall of peptidoglycan

bull plasma membranecytoplasm w cytosol

bull Ribosomenucleoid w DNA

bull draw three basic shapes

bull determine stain reaction

bull Gram positive = purple

bull Gram negative = red

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 6: Introduction microscopy

Other Microscopes

bull 1048673dissectinggreater working distancereflected and transmitted light

bull 1048673light microscope

bull 1048673phase contrast microscope

bull 1048673transmission electron microscope TEM

bull 1048673scanning electron microscope SEM

Cell Theory

bull Cell Theory

bull 1048673all organisms are made of cells

bull 1048673the cell is the smallest living thing

bull 1048673all cells arise from pre-existing cells

Eukaryotic Cells

bull Plant Elodea cells w water

bull draw and label 400X total mag

bull Label nucleus plasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Chloroplastvacuole

Eukaryotic Cells

bull Plant Onion bulb cellsw iodine

bull 1048673draw and label 400X total mag

bull Label nucleus w DNAplasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Nucleolusvacuole

Eukaryotic Cells

bull Animal human cheek cells

bull w methylene blue stain

bull draw and label 400X total mag

bull nucleus w DNA plasma membranecytoplasm w cytosol

Prokaryotic Cells

bull 1048673label cellcell wall of peptidoglycan

bull plasma membranecytoplasm w cytosol

bull Ribosomenucleoid w DNA

bull draw three basic shapes

bull determine stain reaction

bull Gram positive = purple

bull Gram negative = red

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 7: Introduction microscopy

Cell Theory

bull Cell Theory

bull 1048673all organisms are made of cells

bull 1048673the cell is the smallest living thing

bull 1048673all cells arise from pre-existing cells

Eukaryotic Cells

bull Plant Elodea cells w water

bull draw and label 400X total mag

bull Label nucleus plasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Chloroplastvacuole

Eukaryotic Cells

bull Plant Onion bulb cellsw iodine

bull 1048673draw and label 400X total mag

bull Label nucleus w DNAplasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Nucleolusvacuole

Eukaryotic Cells

bull Animal human cheek cells

bull w methylene blue stain

bull draw and label 400X total mag

bull nucleus w DNA plasma membranecytoplasm w cytosol

Prokaryotic Cells

bull 1048673label cellcell wall of peptidoglycan

bull plasma membranecytoplasm w cytosol

bull Ribosomenucleoid w DNA

bull draw three basic shapes

bull determine stain reaction

bull Gram positive = purple

bull Gram negative = red

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 8: Introduction microscopy

Eukaryotic Cells

bull Plant Elodea cells w water

bull draw and label 400X total mag

bull Label nucleus plasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Chloroplastvacuole

Eukaryotic Cells

bull Plant Onion bulb cellsw iodine

bull 1048673draw and label 400X total mag

bull Label nucleus w DNAplasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Nucleolusvacuole

Eukaryotic Cells

bull Animal human cheek cells

bull w methylene blue stain

bull draw and label 400X total mag

bull nucleus w DNA plasma membranecytoplasm w cytosol

Prokaryotic Cells

bull 1048673label cellcell wall of peptidoglycan

bull plasma membranecytoplasm w cytosol

bull Ribosomenucleoid w DNA

bull draw three basic shapes

bull determine stain reaction

bull Gram positive = purple

bull Gram negative = red

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 9: Introduction microscopy

Eukaryotic Cells

bull Plant Onion bulb cellsw iodine

bull 1048673draw and label 400X total mag

bull Label nucleus w DNAplasma membrane

bull cytoplasm w cytosolcell wall of cellulose

bull Nucleolusvacuole

Eukaryotic Cells

bull Animal human cheek cells

bull w methylene blue stain

bull draw and label 400X total mag

bull nucleus w DNA plasma membranecytoplasm w cytosol

Prokaryotic Cells

bull 1048673label cellcell wall of peptidoglycan

bull plasma membranecytoplasm w cytosol

bull Ribosomenucleoid w DNA

bull draw three basic shapes

bull determine stain reaction

bull Gram positive = purple

bull Gram negative = red

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 10: Introduction microscopy

Eukaryotic Cells

bull Animal human cheek cells

bull w methylene blue stain

bull draw and label 400X total mag

bull nucleus w DNA plasma membranecytoplasm w cytosol

Prokaryotic Cells

bull 1048673label cellcell wall of peptidoglycan

bull plasma membranecytoplasm w cytosol

bull Ribosomenucleoid w DNA

bull draw three basic shapes

bull determine stain reaction

bull Gram positive = purple

bull Gram negative = red

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 11: Introduction microscopy

Prokaryotic Cells

bull 1048673label cellcell wall of peptidoglycan

bull plasma membranecytoplasm w cytosol

bull Ribosomenucleoid w DNA

bull draw three basic shapes

bull determine stain reaction

bull Gram positive = purple

bull Gram negative = red

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 12: Introduction microscopy

ModelsStudy both animal and plant cell

modelsbull 1048673

  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18
Page 13: Introduction microscopy
  • Introduction 1048673microscopy
  • Purpose of Microscope
  • Rules and Care of the Microscope abridged
  • Slide 4
  • Slide 5
  • Other Microscopes
  • Cell Theory
  • Eukaryotic Cells
  • Eukaryotic Cells
  • Slide 10
  • Prokaryotic Cells
  • Models Study both animal and plant cell models
  • Slide 13
  • Slide 14
  • Slide 15
  • Slide 16
  • Slide 17
  • Slide 18