INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP ...

INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)

INTERNATIONAL COLLABORATIVE EXERCISE (ICE)

Summary ReportBIOLOGICAL SPECIMEN

2010/2

This report contains the data received from laboratories participating in the current exercise. The results compiled in this report are not intended to be an overview of the quality of work and cannot be interpreted as such. These comments do not reflect the general state of the art within the profession.

Participant results are reported using a randomly assigned "WebCode". This code maintains participant's anonymity, provides linking of the various report sections, and will change with every report.

Table of contents1. Test Sample BS 1....................Page 5

Statement of FindingsQuantative Analysis

2. Test Sample BS 2....................Page 13Statement of FindingsQuantative Analysis

3. Test Sample BS 3....................1Statement of FindingsQuantative Analysis

4. Test Sample BS 4....................Page 29Statement of FindingsQuantative Analysis

Samples Substances Concentrations Comments on substances

Sample 1 [blank sample]

Sample 2 Amfetamine 1380 ng/ml

Sample 3 Methylecgonine 290 ng/ml

Morphine (Total) 920 ng/ml

Morphine and/or Metabolites 0 ng/ml Quantification value given as Morphine (Total)

Benzoylecgonine 580 ng/ml

Sample 4 Alprazolam 810 ng/ml

Test Samples Information

Samples Comments on samples

Sample 1 BS1 contained only lyophilised urine without any drug of abuse included in the ICE menu.

Sample 2 Sample BS2 contained amfetamine as amfetamine sulphate. Urine was spiked with amfetamine sulphate at a concentration of 1880 ng/ml (equivalent to 1380 ng of base/ml) and 50ml aliquots lyophilised.

Sample 3 To prepare BS3, urine was spiked with morphine sulphate (1220 ng/ml, equivalent to 920 ng of base/ml), benzoylecgonine (720 ng/ml, equivalent to 580 ng of base/ml) and ecgonine methyl ester (360 ng/ml, equivalent to 290 ng of base/ml). These analytes were predissolved in water prior to their addition to urine. The urine was then dispensed in 50ml aliquots and lyophilised.

Sample 4 For sample BS4, urine was spiked with alprazolam (810 ng/ml). The analyte was first dissolved in ethanol and then added to the urine. The spiked urine was dispensed in 50 ml aliquots and lyophilised.

2010/2-BS Copyright (c) 2011 UNODC(2)

INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISE (ICE)

Summary Comments

UNODC International Collaborative Exercises (ICE)

An important element of the UNODC International Quality Assurance Programme (IQAP) is the implementation of the International Collaborative Exercises (ICE). The exercises allow laboratories, from both developing and developed countries, to continuously monitor their performance in drug testing on a truly global scale. This report provides information on analytical results of laboratories participating in Biological Specimens group which includes lyophilized (dried) urine samples containing controlled substances and their metabolites and related compounds. In order to maintain confidentiality, the participating laboratories have been assigned random "WebCodes". These codes will change in every round. Two rounds are offered per year with each round presenting participants with four different test samples for analysis .

The analytical results returned by laboratories participating in ICE are evaluated by UNODC and a confidential report is provided to each laboratory on its own performance. Analytical results are reviewed by the UNODC Standing Panel of Forensic Experts which oversees the implementation of these exercises, and offers guidance and support in addressing relevant quality issues. The exercises provide an overview of performance and capacity of participating laboratories and enable UNODC to tailor technical support in the laboratory sector for greatest impact.

Introduction



Codes and Abbreviations

Tech Code Name

100 Agglutination Techniques

110 Enzyme Immunoassay Techniques

120 Fluorescence Polarization Immunoassay

130 Radioimmunoassay

140 Colorimetric reactions

141 Marquis reagent (sulphuric acid, formaldehyde)

142 Cobalt thiocyanate

150 Thin Layer Chromatography

160 High Performance Liquid Chromatography

161 High Performance Liquid Chromatography with diode array detection

170 Gas Chromatography NPD

171 Gas Chromatography FID

172 Gas Chromatography ECD

180 Gas Chromatography/Mass Spectrometry

190 Fourier Transform Infrared Spectrometry

200 Spectrophotometry (visible, UV)

210 Others (specify)

211 NMR

220 Microcrystal test

230 Liquid chromatography/mass spectrometry

231 Liquid chromatography/tandem mass spectrometry

(+) “positive”: Indicates that the analyte is identified; for presumptive tests (e.g. colour reactions), indicates that a positive reaction was obtained.

(-) “negative”: Indicates that the analyte is not identified.

(ANP): Analysis not performed.



Identified substances for Test Sample 1

Code Identified SubstancesDXPIIN metamfetamine

JOOOTO Stimulans

NYXLXU -

ZZXR5W -

Sample 1 Analysis



Code [blank sample]

1NV3E7

4IXYPR On analysis, no common controlled drug was detected in the urine sample.

6YSLUB

88INL8

8TTO8K Sample is negative for drugs

AQKCVV The finding indicates that person did not consume drugs.

B9S6GV

BUPTX1

CS3NSE

DKOHFB In urine sample nº 1, no controled substance was identified

DXPIIN BS-1 found to contain of 10930 ng metamfetamine in 1 ml base

E88H3U no controlled drugs detected, but we found caffeine in this sample.

GPLXQO

HM6CXT

HU0MCM

I7K2BL BS1 was free of analysed controlled drugs

JOOOTO Caffeine

Statement of findings for Test Sample 1



Code [blank sample]

K3WP2Q

LFLIOP

N1A8M1 On analysis, I did not found any common drugs in the urine specimen.

NYXLXU No drugs detected and quantification was not performed.

OKKKEE

P000X0

PB2OVR

QHH888

R44G84

S5KPPP no drug found

TOKPPM

UB733C caffeine

VAVVUA

VIWWYY Negative

VKKNKC

W11111 NO HUBO CONSUMO DE DROGAS

W8MCCC Common drugs not detected



Code [blank sample]

WERFPM

WLZQNY

X77KLJ

XZW3XR

Z696LZ

ZJYZOT

ZZXR5W Test sample 2010/2/BS-1 does not contain controlled drugs.

Response SummaryParticipants: 41



Identification methods for Test Sample 1

Code 100 110 120 150 161 170 171 172 180 230 231 210

1NV3E7 S I SI I

4IXYPR S I I S KIMS

6YSLUB SI

88INL8 S I

8TTO8K S S I

AQKCVV S I

B9S6GV SI S immunochromatography

BUPTX1 S I SI

CS3NSE I S immunochromatographie

DKOHFB I

DXPIIN S I I

E88H3U S I I

GPLXQO S I I

HM6CXT S I I I

HU0MCM S I I I

I7K2BL S I I

JOOOTO I S Dipro Druglab screen

K3WP2Q S I

LFLIOP S S I

N1A8M1 S SI

NYXLXU SI S ELISA

OKKKEE S I I S Remedi HS(TM)

P000X0 S I I I

PB2OVR S I

QHH888 S SI S I

R44G84 SI SI I

S5KPPP SI SI test kit

TOKPPM S I

UB733C S S I I

VAVVUA S I I I I I

VIWWYY S I I

VKKNKC S

W11111 I I SI SI HPTLC

W8MCCC S I

WERFPM S S I

Analytical Technique Codes

Legend: S ... used for Screening I ... used for Identification SI ... used for both



Code 100 110 120 150 161 170 171 172 180 230 231 210

WLZQNY S I S

X77KLJ S I I I

XZW3XR S I S Lateral Flow Immunoassay

Z696LZ S I I I

ZJYZOT S I

ZZXR5W S I

Section 100 110 120 150 161 170 171 172 180 230 231 210

Screening 1 23 8 4 0 1 0 1 9 2 0 7

2.4% 56.1% 19.5% 9.8% 0.0% 2.4% 0.0% 2.4% 22.0% 4.9% 0.0% 17.1%

Identification 0 0 0 4 9 5 2 1 37 4 8 2

0.0% 0.0% 0.0% 9.8% 22.0% 12.2% 4.9% 2.4% 90.2% 9.8% 19.5% 4.9%

# labs using this technique

1 23 8 8 9 5 2 2 37 5 8 7

2.4% 56.1% 19.5% 19.5% 22.0% 12.2% 4.9% 4.9% 90.2% 12.2% 19.5% 17.1%

Response Summary Participants: 41 (combined ... some laboratories used technique for screening and for identification)



False positives for Test Sample 1

Code NameDXPIIN Metamfetamine



Z-Scores for Test Sample 1

Quantification is not required.




Code Identified Substances1NV3E7 amphetamine

4IXYPR Amfetamine

6YSLUB Amfetamina

8TTO8K Afetamine

AQKCVV Amfetamine

B9S6GV amfetamine and acetaminophen

CS3NSE Amphetamine

DKOHFB amfetamine

DXPIIN amfetamine

E88H3U Amphetamine

HM6CXT Amfetamine

HU0MCM Amphetamine

I7K2BL amphetamine and traces of MDMA

JOOOTO Amfphetamine grups, Stimulans

K3WP2Q Amphetamine

N1A8M1 Amphetamine

NYXLXU Amphetamine

P000X0 amfetamine

QHH888 AMPETHAMINE

R44G84 Amfetamine

S5KPPP Amphetamine

UB733C amfetamine

VAVVUA Metamphetamine

VIWWYY Amphetamine

W11111 AMFETAMINE

W8MCCC amphetamine

WERFPM Amphetamine

WLZQNY amphetamine

X77KLJ Amphetamine

XZW3XR Amfetamine

Z696LZ Amfetamin

ZZXR5W amphetamine,methamphetamine

Sample 2 Analysis



Code Amfetamine

1NV3E7 +

The results indicate either the use of amphetamine or not recent use of methamphetamine.

4IXYPR +

On analysis, the urine sample was found to contain the following: Amfetamine 1380 ng/ml

6YSLUB +

88INL8 +

8TTO8K +

Sample is positive for Amfetamine, Paracetamol

AQKCVV +

The finding indicates that person consumed amfetamine.

B9S6GV +

BUPTX1 +

CS3NSE +

DKOHFB +

In urine º 2, amfetamine was identified

DXPIIN +

BS-2 found to contain of 28430 ng amfetamine in 1 ml base

E88H3U +

We found amphetamine as controlled drugs and caffeine in this sample.

GPLXQO -

HM6CXT +

Amfetamine also known as "speed" is a stimulating agent used to not feel fatigue and for its appetite suppressant properties. it is a controlled narcotic in France

HU0MCM +

The sample was found to contain 0.88 microgram/milliliter amphetamine

I7K2BL +

The sample was found to contain amphetamine type stimulants. (amphetamineand traces of MDMA)




Code Amfetamine

JOOOTO +

AmphetamineCaffeine

K3WP2Q +

LFLIOP +

N1A8M1 +

On analysis, I found the urine specimen to containe Amphetamine.

NYXLXU +

Amphetamine was detected in sample BS 2 but quantification was not performed.

OKKKEE +

P000X0 +

taking of amfetamine

PB2OVR +

QHH888 +

R44G84 +

S5KPPP +

Amphetamine 1760.98 ng/ml

TOKPPM +

UB733C +

caffeine

VAVVUA -

1-EIA AND FPIA + as screning test2- Liquid-Liquid extraction,after evaporation to dryness under streem of liquid nitrogen and reconistitution by 100 micro liter of methanol identified and confirmed by GC/NPD And LC/MS/MS

VIWWYY +

VKKNKC ANP

W11111 +

HUBO CONSUMO DE AMFETAMINE



Code Amfetamine

W8MCCC +

1485 ng/ml amphetamine detected.

WERFPM +

POSITIVE FOR AMFETAMINE TYPE STIMULANTS

WLZQNY +

X77KLJ +

Amphetamine could come from the intake of the drug itself or from the metabolism of the other drugs

XZW3XR +

Anfetamine was detected no other drugs such as methamfetamine or drugs metabolizing to amfetamine were identified, this indicate that amfetamine was consumed.

Z696LZ +

This compound, a psychoactive drug, produce feelings of increased energy, euphoria, emotional warmth and distortions time perception and tactile experiences.

ZJYZOT -

ZZXR5W +

Test sample 2010/2/BS-2 contain amphetamine,methamphetamine traces.


Amfetamine: 37




Code 100 110 120 150 161 170 171 172 180 230 231 210

1NV3E7 S I SI I

4IXYPR S I I S KIMS

6YSLUB SI

88INL8 S I

8TTO8K S S I

AQKCVV S I


BUPTX1 S I SI


DKOHFB I

DXPIIN S I I

E88H3U S I I

GPLXQO S I I

HM6CXT S I I I

HU0MCM S I I I

I7K2BL S I I


K3WP2Q S I

LFLIOP S S I

N1A8M1 S SI

NYXLXU SI S ELISA


P000X0 S I I I

PB2OVR S I

QHH888 S SI S I

R44G84 SI SI I


TOKPPM S I

UB733C S S I I

VAVVUA S I I I I I

VIWWYY S I I

VKKNKC S


W8MCCC S I

WERFPM S S I





Code 100 110 120 150 161 170 171 172 180 230 231 210

WLZQNY S I S

X77KLJ S I I I


Z696LZ S I I I

ZJYZOT S I

ZZXR5W S I

Section 100 110 120 150 161 170 171 172 180 230 231 210

Screening 1 23 8 4 0 1 0 1 9 2 0 7

2.4% 56.1% 19.5% 9.8% 0.0% 2.4% 0.0% 2.4% 22.0% 4.9% 0.0% 17.1%

Identification 0 0 0 4 9 5 2 1 37 4 8 2

0.0% 0.0% 0.0% 9.8% 22.0% 12.2% 4.9% 2.4% 90.2% 9.8% 19.5% 4.9%


1 23 8 8 9 5 2 2 37 5 8 7

2.4% 56.1% 19.5% 19.5% 22.0% 12.2% 4.9% 4.9% 90.2% 12.2% 19.5% 17.1%





Code Name88INL8 Morphine and/or Metabolites

VAVVUA Metamfetamine

ZZXR5W Metamfetamine




CODEAmfetamineMAD: 148.2

Conc. * AbsDev ZScore

4IXYPR 1386.0 75.8 0.3

8TTO8K 2061.0 599.2 2.7

B9S6GV 1190.0 271.8 1.2

BUPTX1 1345.0 116.8 0.5

CS3NSE 1300.0 161.8 0.7

DXPIIN 28430.0 26968.2 122.8

HM6CXT 1494.0 32.2 0.1

HU0MCM 885.0 576.8 2.6

JOOOTO 3799.0 2337.2 10.6

K3WP2Q 1610.0 148.2 0.7

OKKKEE 1610.0 148.2 0.7

P000X0 1438.6 23.2 0.1

PB2OVR 1315.0 146.8 0.7

S5KPPP 1761.0 299.2 1.4

UB733C 1295.0 166.8 0.8

VIWWYY 1200.0 261.8 1.2

W11111 1329.0 132.8 0.6

W8MCCC 1485.0 23.2 0.1

WLZQNY 1600.0 138.2 0.6

Z696LZ 1506.7 44.9 0.2

*) Concentration: ng/ml

Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI




Code Identified Substances1NV3E7 morphine, benzoylecgonine, methylecgonine

4IXYPR benzoylecgonine & morphine

6YSLUB benzoylecgonine and morphine

8TTO8K Benzoylecgonine, Methylecgonine, Morphine

AQKCVV Benzoylecgonine, methylecgonine, morphine

B9S6GV morphine, bze and EME

CS3NSE Methylecgonine/Benzoylecgonine/Morphine libre

DKOHFB morphine, benzoylecgonine and methylecgonine

DXPIIN methylecgonine; morphine

E88H3U Morphine and Benzoylecgonine

HM6CXT Morphine, Benzoylecgonine and Methylecgonine

HU0MCM Ecgonine methylester, Benzoylecgonine, morphine

I7K2BL morphine, benzoyl ecgonine, methyl egconine

JOOOTO Cocaine metabol. opiates, Stimulans

K3WP2Q Morphine, Benzoylecgonine, Methylecgonine

N1A8M1 Morphine, Methylecgonine

NYXLXU -

P000X0 benzoylecgonine, methylecgonine and morphine

QHH888 METHYLECGONINE,BENZOYLECGONINE,MORPHINE

R44G84 Benzoylecgonine, Methylecgonine, Morphine

S5KPPP Morphine, codeine, benzoylecgonine, methylecgonine

UB733C benzoylecgonine, methylecgonine, morphine

VAVVUA orphine and Benzoyl ecgonine

VIWWYY Cocaine metabolites and morphine

W11111 Methylecgonine,Benzoilecgonine, Morphine

W8MCCC opiate

WERFPM Morphine, Benzoylecgonine, Methylecgonine

X77KLJ Morphine, benzoilecgonine,metylecgonine

XZW3XR Benzoylecgonine,Methylecgonine, Morphine,6-MAM

Z696LZ Benzoylecgonine, methylecgonine, morphine

ZZXR5W morphine,methylecgonine,benzoilecgonine

Sample 3 Analysis



Code Benzoylecgonine Methylecgonine Morphine (Total) Morphine and/or Metabolites

1NV3E7 + + +

The results indicate either the use of morphine or not recent use of heroin. The presence of benzoylecgonine and methylecgonine indicates the use of cocaine.

4IXYPR + - +

On analysis, the urine sample was found to contain the following: Benzoylecgonine 786 ng/mlMorphine 925 ng/ml

6YSLUB + ANP +

88INL8 + + +

8TTO8K + + +

Sample is positive for Benzoylecgonine, Methylecgonine and Morphine

AQKCVV + + +

The finding indicates that person consumed:1.) cocaine2.) heroin and/or morphine.

B9S6GV + + +

opiates and cocaine

BUPTX1 + + +

CS3NSE + + +

Use of cocaïneUs eof opioides legal or illegal

DKOHFB + + +

In urine º 3, morphine, benzoylecgonine and methylecgonine were identified

DXPIIN - + +

BS-3 found to contain of methylecgonine and morphine (references are not available for quantitative analysis)

E88H3U + ANP +

We found Morphine and Benzoylecgonine as controlled drugs and caffeine in this sample.

GPLXQO - - +

HM6CXT + + +

Benzoylecgonine and methylecgonine are cocaine metabolites. They prove cocaine intake. Morphine is a powerful analgesic agent offen used in first emergency cares, but it's also an abused drug. It's the metabolite of many opiates (heroin, codeine...) These substances are all controlled narcotics in France.





HU0MCM + + +

The sample was found to contain ecgonine methyl ester (0.23 microgram per milliliter), benzoylecgonine (0.73 microgram per milliliter), morphine (0.82 microgram per milliliter)

I7K2BL + + +

The sample was found to contain metabolites of opiates (morphine) and metabolites of cocaine (benzoyl ecgonine, methyl ecgonine)

JOOOTO + + +

Benzoylecgonine, MethylecgonineMorphineCaffeine

K3WP2Q + + +

Morphine as free base and total morphine was in the sample as well.

LFLIOP + + +

N1A8M1 ANP + +

On analysis, I found the urine specimen to containe Amphetamine and Methylecgonine.

NYXLXU ANP ANP ANP

No drugs detected but in screening with ELISA sample contain OPIATEs group.Quantification was not performed.

OKKKEE + + +

P000X0 + + +

taking of cocaine wich is metabolized in BZE and EME. taking of morphine or other opiates (heronie, 6 MAM...) wich is metabolized in morphine

PB2OVR + ANP +

QHH888 + + +

R44G84 + + +

S5KPPP + + +Codeine 261.46 ng/ml, Free morphine 755.05 ng/ml, Total morphine 791.52 ng/ml, Benzoylecgonine 3593.21 ng/ml, Methylecgonine 839.59 ng/ml

TOKPPM - + -

UB733C + + +caffeine




VAVVUA + - +

1-EIA AND FPIA + as screning test2- acidic hydrolysis,Liquid-Liquid extraction,after evaporation to dryness under streem of liquid nitrogen,and derivatization by BSTFA-TMS, confired by GC/MS

VIWWYY + + +

Past use of cocaine and morphine

VKKNKC ANP ANP ANP

W11111 + + +

Hubo consumo de Cocaine y de Morphine

W8MCCC ANP ANP ANP

opiate detected

WERFPM + + +

POSITIVE FOR OPIATES AND COCAINE METABOLITES

WLZQNY + + +

X77KLJ + + +

The detection of cocaine metabolites indicate consumption of cocaine. Morphine could come from the comsumption of the drug itself or from the metabolism of other drugs

XZW3XR + + +

1)Benzoylecgonine and methylecgonine indicates that cocaine was consumed2) Morphine indicates an opiate was consumed and 6-MAM marker of Heroin indicate this opiate was consumed.

Z696LZ + + +

BZE and EME, metabolites of cocaine ate caracteristics of a consumption of cocaine. This compounds are found in urine for 3 days after consumption of cocaine. Cocaine is an alkaloid obtained from leaves of the coca. It is a stimulant of the central nervous system. Toxicity results in cardiac and respiratory failures. Morphine, an opium alkaloid, is a a highly potent analgesic drug with high potential for addiction. It is associated with analgesia, sedation, euphoria, respiratory depression, physical dependence. Thes presence can be caracteristic of a consumption of heroin or morphine.

ZJYZOT - - -

ZZXR5W + + +

Test sample 2010/2/BS-3 contain morphine methylecgonine,benzoilecgonine.


Benzoylecgonine: 33

Methylecgonine: 31

Morphine and/or Metabolites: 36




Code 100 110 120 150 161 170 171 172 180 230 231 210

1NV3E7 S I SI I

4IXYPR S I I S KIMS

6YSLUB SI

88INL8 S I

8TTO8K S S I

AQKCVV S I


BUPTX1 S I SI


DKOHFB I

DXPIIN S I I

E88H3U S I I

GPLXQO S I I

HM6CXT S I I I

HU0MCM S I I I

I7K2BL S I I


K3WP2Q S I

LFLIOP S S I

N1A8M1 S SI

NYXLXU SI S ELISA


P000X0 S I I I

PB2OVR S I

QHH888 S SI S I

R44G84 SI SI I


TOKPPM S I

UB733C S S I I

VAVVUA S I I I I I

VIWWYY S I I

VKKNKC S


W8MCCC S I

WERFPM S S I





Code 100 110 120 150 161 170 171 172 180 230 231 210

WLZQNY S I S

X77KLJ S I I I


Z696LZ S I I I

ZJYZOT S I

ZZXR5W S I

Section 100 110 120 150 161 170 171 172 180 230 231 210

Screening 1 23 8 4 0 1 0 1 9 2 0 7

2.4% 56.1% 19.5% 9.8% 0.0% 2.4% 0.0% 2.4% 22.0% 4.9% 0.0% 17.1%

Identification 0 0 0 4 9 5 2 1 37 4 8 2

0.0% 0.0% 0.0% 9.8% 22.0% 12.2% 4.9% 2.4% 90.2% 9.8% 19.5% 4.9%


1 23 8 8 9 5 2 2 37 5 8 7

2.4% 56.1% 19.5% 19.5% 22.0% 12.2% 4.9% 4.9% 90.2% 12.2% 19.5% 17.1%





Code NameS5KPPP Codeine

XZW3XR 6-Monoacetylmorphine (6-MAM)




CODEBenzoylecgonineMAD: 101.9

MethylecgonineMAD: 65.6

Conc. * AbsDev ZScore Conc. * AbsDev ZScore

4IXYPR 786.0 16.0 0.1

8TTO8K 717.0 85.0 0.6 358.0 55.0 0.6

B9S6GV 860.0 58.0 0.4 303.0 0.0 0.0

BUPTX1 834.0 32.0 0.2 370.9 67.9 0.7

CS3NSE 802.0 0.0 0.0 258.0 45.0 0.5

HM6CXT 961.0 159.0 1.1 290.0 13.0 0.1

HU0MCM 737.0 65.0 0.4 232.0 71.0 0.7

K3WP2Q 892.0 90.0 0.6 304.0 1.0 0.0

OKKKEE 693.0 109.0 0.7 1036.0 733.0 7.5

P000X0 725.3 76.7 0.5 368.6 65.6 0.7

PB2OVR 563.0 239.0 1.6

S5KPPP 3593.2 2791.2 18.5 839.6 536.6 5.5

UB733C 545.0 257.0 1.7 268.0 35.0 0.4

VIWWYY 700.0 102.0 0.7 210.0 93.0 1.0

W11111 1305.0 503.0 3.3 180.0 123.0 1.3

WLZQNY 1100.0 298.0 2.0 220.0 83.0 0.9

Z696LZ 903.9 101.9 0.7 326.2 23.2 0.2






Code Identified Substances1NV3E7 alprazolam

4IXYPR Alprazolam

8TTO8K Alprazolam

AQKCVV Alprazolam, diazepam

B9S6GV alprazolam

CS3NSE Alprazolam

DKOHFB alprazolam

DXPIIN alprazolam

E88H3U Alprazolam

HM6CXT Alprazolam

HU0MCM Alprazolam

I7K2BL alprazolam

JOOOTO Benzodiazepines, Stimulans

K3WP2Q Alprazolam

N1A8M1 Alprazolam

NYXLXU Alprazolam

P000X0 alprazolam

QHH888 ALPRAZOLAM

R44G84 Alprazolam

S5KPPP Alprazolam

UB733C alprazolam

VAVVUA Alprazolam

W11111 Alprazolam

W8MCCC alprazolam

WERFPM Alprazolam

X77KLJ Alprazolam

XZW3XR Alprazolam

Z696LZ Alprazolam

ZZXR5W benzodiazepines

Sample 4 Analysis



Code Alprazolam

1NV3E7 +

The results indicate the use of alprazolam.

4IXYPR +

On analysis, the urine sample was found to contain the following: Alprazolam 742 ng/ml

6YSLUB ANP

88INL8 +

8TTO8K +

Sample is positive for Alprazolam

AQKCVV +

The finding indicates that person consumed benzodiazepines:1.) alprazolam2.) diazepam.

B9S6GV +

benzodiazepines

BUPTX1 +

CS3NSE +Use of Alprazolam

DKOHFB +

In urine º 2, alprazolam was identified

DXPIIN +

BS-4 found to contain of 19740 ng alprazolam in 1 ml base

E88H3U +

We found Alprazolam as controlled drugs and caffeine in this sample.

GPLXQO +

HM6CXT +

Alprazolam is an anxiolytic benzodiazepine. It can also be used in the prevention and treatment of delirium tremens and alcoholism.

HU0MCM +

The sample was found to contain 0.68 microgram/milliliter alprazolam

I7K2BL +

The sample was found to contain a benzodiazepine (alprazolam).




Code Alprazolam

JOOOTO +

AlprazolamCaffeine

K3WP2Q +

LFLIOP +

N1A8M1 +

On analysis, I found the urine specimen to containe Alprazolam.

NYXLXU +

Alprazolam was detected in sample BS 4 but quantification was not performed.

OKKKEE +

P000X0 +

taking of drugs contained alprazolam

PB2OVR +

QHH888 +

R44G84 +

S5KPPP +

Alprazolam 596.49 ng/ml

TOKPPM -

UB733C +

caffeine

VAVVUA +

1-EIA AND FPIA + as screning test2- Liquid-Liquid extraction,after evaporation to dryness under streem of liquid nitrogen and reconistitution by 100 micro liter of methanol identified and confirmed by GC/FID And LC/MS/MS

VIWWYY ANP

Negative

VKKNKC ANP

W11111 +

Hubo consumo de Alprazolam



Code Alprazolam

W8MCCC +

alprazolam detected

WERFPM +

POSITIVE FOR BENZODIAZEPINES

WLZQNY +

X77KLJ +

The result could come from intake of the drug itself

XZW3XR +

In the sample was detected Alprazolam, this finding indicate that Alprazolam was consumed.

Z696LZ +

Alprazolam is psychoactive drug and is used for their hypnotic, sedative, anxiolytic, anticonvusant and muscle relaxant properties.

ZJYZOT -

ZZXR5W ANP

Test sample 2010/2/BS-4 contain bezodiazepines.


Alprazolam: 35




Code 100 110 120 150 161 170 171 172 180 230 231 210

1NV3E7 S I SI I

4IXYPR S I I S KIMS

6YSLUB SI

88INL8 S I

8TTO8K S S I

AQKCVV S I


BUPTX1 S I SI


DKOHFB I

DXPIIN S I I

E88H3U S I I

GPLXQO S I I

HM6CXT S I I I

HU0MCM S I I I

I7K2BL S I I


K3WP2Q S I

LFLIOP S S I

N1A8M1 S SI

NYXLXU SI S ELISA


P000X0 S I I I

PB2OVR S I

QHH888 S SI S I

R44G84 SI SI I


TOKPPM S I

UB733C S S I I

VAVVUA S I I I I I

VIWWYY S I I

VKKNKC S


W8MCCC S I

WERFPM S S I





Code 100 110 120 150 161 170 171 172 180 230 231 210

WLZQNY S I S

X77KLJ S I I I


Z696LZ S I I I

ZJYZOT S I

ZZXR5W S I

Section 100 110 120 150 161 170 171 172 180 230 231 210

Screening 1 23 8 4 0 1 0 1 9 2 0 7

2.4% 56.1% 19.5% 9.8% 0.0% 2.4% 0.0% 2.4% 22.0% 4.9% 0.0% 17.1%

Identification 0 0 0 4 9 5 2 1 37 4 8 2

0.0% 0.0% 0.0% 9.8% 22.0% 12.2% 4.9% 2.4% 90.2% 9.8% 19.5% 4.9%


1 23 8 8 9 5 2 2 37 5 8 7

2.4% 56.1% 19.5% 19.5% 22.0% 12.2% 4.9% 4.9% 90.2% 12.2% 19.5% 17.1%





Code NameAQKCVV Diazepam




CODEAlprazolamMAD: 112.0

Conc. * AbsDev ZScore

4IXYPR 742.0 0.0 0.0

8TTO8K 830.0 88.0 0.5

B9S6GV 789.0 47.0 0.3

BUPTX1 1247.2 505.2 3.0

CS3NSE 1019.0 277.0 1.7

DXPIIN 19740.0 18998.0 114.5

HM6CXT 608.0 134.0 0.8

HU0MCM 686.0 56.0 0.3

K3WP2Q 630.0 112.0 0.7

P000X0 829.7 87.7 0.5

S5KPPP 596.5 145.5 0.9

UB733C 420.0 322.0 1.9

W11111 158.0 584.0 3.5

WLZQNY 790.0 48.0 0.3

Z696LZ 724.0 18.0 0.1





The Laboratory and Scientific SectionUNODC, International CentreWagramer Strasse 5, P.O. Box 500A-1400, Vienna, AUSTRIAEmail: [email protected]



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