Instrumental Analysis, Laboratory Oral Presentation
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Transcript of Instrumental Analysis, Laboratory Oral Presentation
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Instrumental Analysis, LaboratoryOral PresentationNikko BacalzoDouglas DyJeremy LyFrance Tan
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Determination of Anions in Toothpaste by Ion Chromatography Instrument Used:Ion Chromatography
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FLUORIDE
http://toxicfreekidsblog.org/2011/07/toxic-toothpaste/http://www.webmd.com/oral-health/image-collection-oral-health#phototake_rm_photo_of_tooth_illustration.jpg
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http://www.skepticnorth.com/2010/10/fluoridiated-water-debate-returns-nothing-new-is-said/http://www.fluoridation.com/teeth.htm
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ION CHROMATOGRAPHY
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ION CHROMATOGRAPHY
http://www.separations.us.tosohbioscience.com/ServiceSupport/TechSupport/ResourceCenter/PrinciplesofChromatography/IonExchange
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ION CHROMATOGRAPHY
Injection valve
Readout
Mobile phase/ solvent reservoir Pump Column Detector
Excess/waste
Filter
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Data and Results
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Data and Results
0 5 10 15 20 25 30 350
50000
100000
150000
200000
250000
300000
350000
f(x) = 21381.584705256 x + 50726.6666666663R² = 0.892850575318569
concentration (ppm)
peak
are
a
Fluoride calibration
curve
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Data and Results
0 5 10 15 20 25 30 35 40 450
50000
100000
150000
200000
250000
f(x) = 31566.7370857077 x − 30763.6666666669R² = 0.861303259627165
concentration (ppm)
peak
are
a
Chloride calibration
curve
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Data and Results
BrandIon n
%(w/w) mean ± std dev
%RSD
Sensodyne
F 3 -- --
Cl 2 a0.00923 ± 0.00002
0.270
Colgate F 3 -- b --Cl 3 -- --
HapeeF 3 -- --Cl 2 a 0.016 ± 0.002 15.3
Close-Up
F 3 -- --Cl 3 -- --
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Data and Resultsconc
(ppm)spiked (mg)
unspiked (mg)
added
(mg)
%recovery
SPIKE 40.62 2.031 0.02052 1.344 149.6
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Conclusion• Four toothpaste brands were analyzed for their fluoride
and chloride contents (Sensodyne, Colgate, Close-Up and Hapee).
• Standards were run in the ion chromatograph, and the retention times and the concentration-peak area relationships of each were drawn from the data.
• Out of which only Sensodyne and Hapee showed quantifiable amounts of chloride in the toothpaste formulation.
• Calculation yielded concentrations of 0.00923% and 0.016% chloride in both toothpastes, respectively.
• Other samples had no sufficiently quantifiable amounts of both anions, suspected to be consequence of matrix effect.
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Recommendations• Use other MP: carbonate/bicarbonate buffer, KOH• Use gradient elution• Standard addition instead of calibration curve• Homogenization of samples• Analyze other anions (MFP, benzoate, sulfate,
phosphate)• Method validation
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References• Takahara, Y, et al. Photooxidation of organic
compounds in a solution containing hydrogen peroxide and TiO2 particles under visible light. Journal of Applied Chemistry. Vol.35, 793-797. <http://www.springerlink.com/content/t866330021785302/>
• Shimadzu Corporation. The UV-2401PC: A Superbly Engineered Instrument. Accessed on May 21, 2012. <http://www.pa.ibf.cnr.it/web/pdf/12.pdf>
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Thank you for listening