Inhibition of gene expression by means of nucleic...
Transcript of Inhibition of gene expression by means of nucleic...
![Page 1: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/1.jpg)
Prof. Józef Dulak, PhD, DScDepartment of Medical Biotechnology
Faculty of Biochemistry, Biophysics and BiotechnologyRoom 3.025/3.07 Phone 664-63-75
Email: [email protected]
15th December 2014
Inhibition of gene expressionby means of nucleic acids
Lecture 9
![Page 2: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/2.jpg)
Genetic therapy
Acquired diseases
Inherited diseases Acquired diseases
Gene addition Gene inhibition Gene repairSubstitutingdelivery of
proper version of mutated gene
Enhancingdelivery of gene which
expression isimpaired
Suppresivedelivery of gene killing
the cells
Silencing ofgene expressionwith non-coding
nucleic acids(antisenseDNA decoyssiRNA/shRNA
microRNA)
Correction ofthe mutated
gene
![Page 3: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/3.jpg)
DNA RNA
Genes(delivered by DNA vectors)
Non-coding sequences
Antisense oligonucleotides
DNA decoys
Genes(delivered by RNA vectors)
Non-coding sequences
ribozymes siRNAmicroRNA
Therapeutic nucleic acids
3
![Page 4: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/4.jpg)
Watson-Crick based-dependent hybridisation is crucial for the inhibitory activity of several classes of inhibitory nucleic acid
However, this is not the only way in which nucleic acids caninhibit gene expression
4
![Page 5: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/5.jpg)
Hybridisation of nucleic acids as a way to inhibitgene expression
DNA hybridizeswith DNA DNA hybridizes
with RNA RNA hybridizes
with RNA
U
U
U
U
U
U
U
U
5
![Page 6: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/6.jpg)
Inhibitory nucleic acids
1. Antisense oligonucleotides: short, 12-20 nts. 2. Triple helix-forming oligonucleotides (TFO)
pyrmidine oligodeoxynucleotides that specifically bind to a major groove of polypurine region of dsDNA via the formation of triple helices according to recognition rules established by Hoogsten
3. Ribozymes – short catalytically active RNSs4. Deoxyribozymes (DNAzymes) – short catalytic DNA that
cleave sequence-specifically targeted RNA. More stable than RNA, it is easier to synthesize and to modify them.
5. siRNA/microRNA
I. Nucleic acids that bind to other nucleic acid
II. Nucleic acids that bind to proteins
6
![Page 7: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/7.jpg)
Antisense oligonucleotides
7
![Page 8: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/8.jpg)
Antisense oligonucleotidesShort fragments of single strand, chemically modified
DNA nucleotides (oligonucleotides), complementary to a given mRNA.
Paul Zamecnik (b. 1912)
discoverer of tRNA died October 2009
Later in his career, Zamecnik and Stephenson developed antisense technology, in which short, synthetic nucleotide sequences can be used to silence the activity of individual genes. They published their results, in which they used a 13-nucleotide sequence to halt production of Rous sarcoma virus in chicken embryos, in 1978. That paper appeared in Proceedings of the National Academy of Sciences,
8
![Page 9: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/9.jpg)
Antisense oligonucleotides
9
![Page 10: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/10.jpg)
Antisense oligonucleotides
10
![Page 11: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/11.jpg)
The enzyme RNase H (EC 3.1.26.4) is a ribonuclease that cleaves the 3'-O-P-bond ofRNA in a DNA/RNA duplex to produce 3'-hydroxyl and 5'-phosphate terminated
products. RNase H is a non-specific endonuclease and catalyzes the cleavage of RNA via a hydrolytic mechanism, aided by an enzyme-bound divalent metal ion.
Members of the RNase H family can be found in nearly all organisms, from archaeaand prokaryota to eukaryota . In eukaryotic DNA replication RNase H is
responsible for cutting out the RNA primer, allowing completion of the newly synthesized DNA
RNAse H
11
![Page 12: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/12.jpg)
Antisense oligonucleotides
- 13-25 nucleotides long - hybridize to corresponding RNA - act by: a) modulation of splicing
b) inhibition of protein translation by disruption of protein assembly
c) utilise RNAse H enzymes
12
![Page 13: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/13.jpg)
Oligonucleotides fate in vivo
1.Degradation by serum nucleases 2. Degradation by liver cells (unspecific uptake) 3. Degradation by kidney cells and excretion with urine
13
![Page 14: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/14.jpg)
Modification of antisense oligonucleotide
1st generation
14
![Page 15: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/15.jpg)
Clinical application of antisense oligonucleotides
Vitravene - CMV-induced retinitis
Bcl2 antisense - melanoma
15
![Page 16: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/16.jpg)
Application of antisense nucleotides for DNA repair strategies -
To be disucssed in January
![Page 17: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/17.jpg)
DNA RNA
Genes(delivered by DNA vectors)
Non-coding sequences
Antisense oligonucleotides
DNA decoys
Genes(delivered by RNA vectors)
Non-coding sequences
ribozymes siRNAmicroRNA
Therapeutic nucleic acids
17
![Page 18: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/18.jpg)
DNA decoys
Pułapki oligonukleotydowe
18
![Page 19: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/19.jpg)
DNA decoys
19
![Page 20: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/20.jpg)
DNA decoys
DNA decoys – inhibit transcription
Antisense – inhibit translation
DNA decoys – double strandedDNA fragments binding the specific transcription factor
20
![Page 21: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/21.jpg)
Narrowing of blood vessels after angioplastyor CABG
narrowing occurs also in vessels used for by-pass grafting
- STENOSIS
RESTENOSIS
21
![Page 22: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/22.jpg)
cdkCyclin A Rb
E2F
TTTCGCGC
c-mycc-mybcdc2 kinasePCNA
silent
cdkCyclin A Rb
E2FTTTCGCGC
c-mycc-mybcdc2 kinasePCNA
transactivate
P
Transcription factor E2F
Mann MJ, Mol Med. Today 2000
Quiescence
Proliferation
E2F induces proliferation of vascular smooth muscle cells what maycause vessel narrowing
22
![Page 23: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/23.jpg)
cdkCyclin A Rb
E2FTTTCGCGC
c-mycc-mybcdc2 kinasePCNA
transactivate
P
cdkCyclin A RbE2F
TTTCGCGC
c-mycc-mybcdc2 kinasePCNA
silent
P
DNA decoy
TTTCGCGC
TTTCGCGC
TTTCGCGC
TTTCGCGC
Mann MJ, Mol Med. Today 2000
Inhibition of cell proliferation by E2F decoys
23
![Page 24: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/24.jpg)
Testing an Edifoligide, an E2F-decoy in preventionof bypass graft stenosis
Mann et al., Lancet. 1999 Oct 30;354(9189):1493-8.
![Page 25: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/25.jpg)
Failure of phase III trials with E2F decoys...
edifoligide failed to show any benefit for primary and secondary end points in two phase III trials...
In the PREVENT III trial of 1,404 patients with critical limb ischemia who needed peripheral artery bypass graft surgery, there was no difference between edifoligide and placebo on the primary end point of limb amputation. No differences were seen in secondary end points of critical graft stenosis, recurrent limb ischemia, or quality of life.
Similar results were reported in the PREVENT IV trial, which tested edifoligide against placebo in 3,014 patients for the prevention of vein graft failure after coronary artery bypass surgery.
Multiple isoforms of E2F exist, and the drug may not have inhibited them all. Edifoligide's pharmacokinetics may not have allowed it to inhibit E2F adequately
Reasons?
JAMA 2005, November, 294: 2495-2497 25
![Page 26: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/26.jpg)
Macular degeneration
26
![Page 27: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/27.jpg)
Types of AMD AMD is the #1 cause of severe vision loss in people over age 60. Today, at least 15 million people in the United States have this health problem.
Dry AMD is the most common form of AMD, representing approximately 90% of all AMD cases. However, dry AMD accounts for only 10% of the severe vision loss associated with macular degeneration. Dry AMD is characterized by development of yellow-white deposits underneath your retina, known as drusen, and can also be determined by deterioration of your retina. There is no generally accepted treatment for dry AMD, although vitamins, antioxidants and zinc supplements may slow its progression. Over time, dry AMD cases often develop into wet macular degeneration
Wet AMD occurs when abnormal blood vessels start to grow under the center of your retina. These new blood vessels may be very fragile and often leak blood and fluid. The blood and fluid can damage your macula or create a scar on your retina, causing vision problems. Damage to the macula can occur rapidly, causing a noticeable blurring or even loss of central vision. The vision loss may be permanent, because abnormal blood vessels and scar tissue are actually destroying normal retina tissue. Once lost, these light-sensitive cells in your retina cannot be replaced.
It is estimated that about 1.6 million people in the United States currently have wet AMD, with 200,000 new cases per year.
27
![Page 28: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/28.jpg)
VEGF-A – a major mediator of blood vessels formation
Receptors on endothelial
cells
VEGF-A is a major angiogenic growth factor. It acts on endothelial cells, being produced by
numerous cell types, including vascular smooth muscle cells (VSMC), fibroblasts or tumor cells.
![Page 29: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/29.jpg)
Various isoforms of VEGF
Exons 1-5 8
Exons 1-5 86A1 A2
Exons 1-5 87
VEGF-A121
VEGF-A145
VEGF-A165
VEGF-A189
VEGF-A206
Exons 1-5 876A1 A2
Exons 1-5 876A1 A2 6B
VEGF165 isoform appears to be the most important29
![Page 30: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/30.jpg)
Treatment for AMD
1. Photodynamic therapy
1. Antibodies – anti-VEGF2.1. Bevacizumab (Avastin) 2.2. Rivacizumab (Lucentis)
3. Aptamers – pegaptanib sodium (Macugen)
30
![Page 31: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/31.jpg)
MACUGEN (PEGAPTANIB)
Pegaptinib sodium is a pegylated oligonucleotide aptamer that binds to and inactivates VEGF165
31
![Page 32: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/32.jpg)
Pegaptanib sodiumPegaptanib sodium is a covalent conjugate of an oligonucleotide of twenty-eight nucleotides in length that terminates in a pentylamino linker, to which two 20-kilodalton monomethoxy polyethylene glycol (PEG) units are covalently attached via the two amino groups on a lysine residue.
The chemical name for pegaptanib sodium is as follows: RNA, ((2'-deoxy-2'-fluoro)C-Gm-Gm-A-A-(2'-deoxy-2'-fluoro)U-(2'-deoxy-2' -fluoro)C-Am-Gm-(2'-deoxy-2-fluoro)U-Gm-Am-Am-(2'-deoxy-2' -fluoro)U-Gm-(2'-deoxy-2'-fluoro)C-(2'-deoxy-
2'-fluoro)U-(2'-deoxy-2'-fluoro)U-Am-(2'-deoxy-2' -fluoro)U-Am-(2'-deoxy-2'-fluoro)C-Am-(2'-deoxy-2'-fluoro)U-(2'-deoxy-2'-fluoro)C-(2'-deoxy-2' -fluoro)C-Gm-(3'® 3')-dT), 5'-ester with a ,a '-[4,12-dioxo-6-[[[5-
(phosphoonoxy)pentyl]amino]carbonyl]-3,13-dioxa-5,11-diaza-1,15-pentadecanediyl]bis[w -methoxypoly(oxy-1,2-ethanediyl)], sodium salt.
The molecular formula for pegaptanib sodium is : C294H342F13N107Na28O188P28 [C2H4O]n
32
![Page 33: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/33.jpg)
Macugen
-registered by FDA in December 2004
MACUGEN treatment given every 6 weeks for up to 2 years has been shown to significantly reduce the risk of moderate vision loss in patients with all types of wet AMD.
- Specifically binds VEGF-A165
administered by intravitreal injection every sixweeks for at least two years
33
![Page 34: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/34.jpg)
Aptamers
1. DNA decoys – bind transcription factors
Nucleic acids that bind proteins
Exist naturally – produced by viruses (HIV, adenoviruses)
2. Pegaptinib sodium – a pegylated oligonucleotide binding VEGF165
34
![Page 35: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/35.jpg)
DNA RNA
Genes(delivered by DNA vectors)
Non-coding sequences
Antisense oligonucleotides
DNA decoys
Genes(delivered by RNA vectors)
Non-coding sequences
ribozymes siRNAmicroRNA
Therapeutic nucleic acids
35
![Page 36: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/36.jpg)
RibozymesSmall RNA molecules which allow sequence-specific endoribonucleolytic
cleavage in a catalytic manner
Processing of rRNA in Tetrahymena
Self-cleaving RNAs from: - bacteriophages - plant satellite virusoids - satellite tobacco ringspot virus - human delta hepatitis virus
RNA components of a polysaccharide branching enzyme and RNAse P
Discovered in the begining of 80’s by Thomas Cech and Sydney Altman
36
![Page 37: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/37.jpg)
„Hammerhead” ribozymes
-They are present in genomes of viroids and virusoids, small single-stranded RNA pathogens of plants
- they are built from three helices connected by loops- modified ribozymes have only one loop
37
![Page 38: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/38.jpg)
H- A, C lub U
„Hammerhead” ribozymes
38
![Page 39: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/39.jpg)
Correction of mutation by means of ribozymes
/correct exon
mutated exon
complementary sequence allowing for hybridisation of ribozyme to mRNA
recovered, correct mRNA
Szala et al., Terapia genowa, 2003 39
![Page 40: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/40.jpg)
Delivery of ribozymes to target cells
- exogenous - ribozymes must be chemically modified - endogenous - coding sequences for ribozymes are delivered
with viral/plasmid vectors
Degradation of ribozymes in the serum
Ribozymes are currently not tested widely for therapeutic purposes
40
![Page 41: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/41.jpg)
RNA interference
PSTG – post-transciptional gene silencing
Specific inhibition of gene expression by double-stranded RNA, which stimulates the degradation of a target mRNA
41
![Page 42: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/42.jpg)
Jorgensen i wsp, 1990
chalcone
![Page 43: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/43.jpg)
THE PLANT CELL, Vol 2, Issue 4 279-289, Copyright © 1990 by American Society of Plant Biologists
Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans
C. Napoli, C. Lemieux and R. Jorgensen DNA Plant Technology Corporation, 6701 San Pablo Avenue, Oakland, California 94608
We attempted to overexpress chalcone synthase (CHS) in pigmented petunia petals by introducing a chimeric petunia CHS gene. Unexpectedly, the introduced gene created a block in anthocyanin biosynthesis. Forty-two percent of plants with the introduced CHS gene produced totally white flowers and/or patterned flowers with white or pale nonclonal sectors on a wild-type pigmented background; none of hundreds of transgenic control plants exhibited such phenotypes. Progeny testing of one plant demonstrated that the novel color phenotype co-segregated with the introduced CHS gene; progeny without this gene were phenotypically wild type. The somatic and germinal stability of the novel color patterns was variable. RNaseprotection analysis of petal RNAs isolated from white flowers showed that, although the developmental timing of mRNA expression of the endogenous CHS gene was not altered, the level of the mRNA produced by this gene was reduced 50-fold from wild-type levels. Somatic reversion of plants with white flowers to phenotypically parental violet flowers was associated with a coordinate rise in the steady-state levels of the mRNAs produced by both the endogenous and the introduced CHS genes. Thus, in the altered white flowers, the expression of both genes was coordinately suppressed, indicating that expression of the introduced CHS gene was not alone sufficient for suppression of endogenous CHS transcript levels. The mechanism responsible for the reversible co-suppression of homologous genes in trans is unclear, but the erratic and reversible nature of this phenomenon suggests the possible involvement of methylation.
43
![Page 44: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/44.jpg)
dsRNA inhibits gene expression more effectively than ssRNA
Control Embryo
Embryo with Visualised
mRNA of mex-3
Embryo injected with single-stranded antisense RNA
Embryo injected with dsRNA
Fire at al., 1998
dsRNA several hundred nucleotides long
Nematode after injection of unrelated dsRNA
Nematode injected with specific dsRNA
![Page 45: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/45.jpg)
- RNA interference is an inherited mechanism
- Only few dsRNA were required for inhibition of gene expression, indicating for an amplification process
DsRNA is more effective than single stranded
45
![Page 46: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/46.jpg)
Nobel prize 2007
Andrew Fire Craig Mello 46
![Page 47: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/47.jpg)
Genetic inhibition by double-stranded RNAAbstract
A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo. The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA
and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods
Inventors: Fire; Andrew (Baltimore, MD), Kostas; Stephen (Chicago, IL), Montgomery; Mary (St. Paul, MN), Timmons; Lisa (Lawrence, KS), Xu; SiQun (Ballwin, MO),
Tabara; Hiroaki (Shizuoka, JP), Driver; Samuel E. (Providence, RI), Mello; Craig C. (Shrewsbury, MA)
Assignee: Carnegie Institute of Washington (Washington, DC)
Appl. No. 09/215,257
Filed: December 18, 1998
This application claims the benefit of U.S. Provisional Appln. No. 60/068,562, filed Dec. 23, 1997.
Patent for RNA interference
47
![Page 48: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/48.jpg)
Mechanisms of RNA interference
Dicer – an enzyme that cleaves long dsRNA
BL Davidson & PB McCray Jr, Nature Rev Genet, 201148
![Page 49: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/49.jpg)
49
![Page 50: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/50.jpg)
Methods for delivery of RNAi triggers
BL Davidson & PB McCray Jr, Nature Rev Genet, 201150
![Page 51: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/51.jpg)
Strategies for delivery of siRNA in vivo
51
![Page 52: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/52.jpg)
Some clinical trials for RNAi therapy
Z. Li & T. Rana, Nature Reviews Drug Discovery, August 2014
![Page 53: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/53.jpg)
microRNA
53
![Page 54: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/54.jpg)
K. Goljanek –Whysall et al., Clinical Science 2012
microRNAs regulate mRNA expression
Some microRNAs are encoded in introns on mRNA genes
![Page 55: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/55.jpg)
Marquez & McCaffrey, Hum Gene Therapy 2008
The microRNA/RNA interference pathway
55
![Page 56: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/56.jpg)
microRNA
1. Regulate the expression of about 50% (or more…) mRNA genes
2. Regulation is strong and dependent on endogenous RNA interference system
3. microRNAs target specific sequences in mRNA
4. microRNAs are often expressed in a cell-specific manner
5. microRNA targeted vectors may allow cell-specific regulation of gene expression
![Page 57: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/57.jpg)
• it is well documented that an individual miRNA can influence hundreds of gene transcripts to coordinate complex programs of gene expression and thereby, affect global changes in the physiologyof a cell
• miRNAs have been implicated as key molecular players in virtually all the cellular processes,
• numerous studies have demonstrated that alterations in the spectrum of miRNAs are correlated with various pathologicalsituations
microRNAs: a few basic facts
![Page 58: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/58.jpg)
Exploiting and antagonizing microRNA regulation for
therapeutic and experimental applications
58
![Page 59: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/59.jpg)
Cell-specific regulation of gene expression
1. Cell specific promoters
-eg, Flk-1, Tie -2: endothelial-specific
-Keratin 14 – keratinocyte specific
2. Vectors inhibited by microRNA
In 3’ region of the transgene the sequence recognized by microRNA expressed in the cells, in which we do not want to express the transgene is incorporated
eg. if we want the expression in hepatocyte but not in Kuppfer cell, we haveto add to the 3’ mRNA of the transgene the sequence recognized specifically by microRNA expressed in Kuppfer cells
![Page 60: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/60.jpg)
Naldini i wsp. 2009
microRNA regulated vectors to drive expressionin a specific cell
![Page 61: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/61.jpg)
Inborn errors of metabolism – Krabbe’s disease
![Page 62: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/62.jpg)
Krabbe’s disease
From: Wikipedia
![Page 63: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/63.jpg)
Krabbe’s disease
![Page 64: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/64.jpg)
Psychosine is responible for demyelination in Krabbe’s disease
![Page 65: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/65.jpg)
Krabbe’s disease
![Page 66: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/66.jpg)
Treatment of globoid-cell leukodystrophy
![Page 67: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/67.jpg)
microRNA-regulated gene therapy of globoid cell lecukodystrophy
![Page 68: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/68.jpg)
microRNA-regulated gene therapy of globoid cell lecukodystrophy
![Page 69: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/69.jpg)
Gene therapy for Krabbe’s disease
Orchard & Wagner, NEJM 2011
Gertner/Naldini, Science Transl Med. 2010
1. Krabbe’s disease- lack of galactocerebrosidase2. GALC can be delivered by HSC3. However, GALC is toxic for early HSC
![Page 70: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/70.jpg)
Therapeutic efficacy of Krabbe’s disease gene therapy
![Page 71: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/71.jpg)
microRNAs for genetic therapies
![Page 72: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/72.jpg)
miR-34a as tumor suppressor microRNA
![Page 73: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/73.jpg)
Hepatitis C
• An RNA virus discovered 25 years ago
• Major cause of liver cancer and liver transplant
• Globally 350,000 people die of HCV
• In USA more people die of HCV than AIDS
• Treatment: ribavirin and interferon – but with side effects: fever, headaches, fatigue,depression and anemia
• Therapy lasts up to 11 months and clears the infection in 5—70 %
• Current therapies: addition of protease inhibitors – improves cure rates and lessenstreatment time – but can be applied only against the most common type of HCV(so not equally effective around the world)
![Page 74: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/74.jpg)
Role of miR-122 in HCV replication
HL Janssen et al., NEJM , 3 May 2013
miR-122, by binding to HCV RNA, promotes its propagation
![Page 75: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/75.jpg)
Miravirsen – targeting HCV infection
HL Janssen et al., NEJM , 3 May 2013
![Page 76: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/76.jpg)
Miravirsen – targeting HCV infection
N Engl J Med. 2013 May 2;368(18):1685-94. doi: 10.1056/NEJMoa1209026. Epub 2013 Mar 27
![Page 77: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/77.jpg)
Miravirsen – targeting HCV infection
N Engl J Med. 2013 May 2;368(18):1685-94. doi: 10.1056/NEJMoa1209026. Epub 2013 Mar 27
![Page 78: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/78.jpg)
Miravirsen – targeting HCV infection
N Engl J Med. 2013 May 2;368(18):1685-94. doi: 10.1056/NEJMoa1209026. Epub 2013 Mar 27
![Page 79: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/79.jpg)
Selected anti-miR therapeutics curently in development
Z. Li & T. Rana, Nature Reviews Drug Discovery, August 2014
![Page 80: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/80.jpg)
Oligonucleotide manipulation of microRNAfunction
Small & Olson, Nature 201180
![Page 81: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/81.jpg)
Different ways of inhibition of gene expression
/miRNA
S. Richards., The Scientist, 03.2012
DNA decoys (inhibit transcription)
81
![Page 82: Inhibition of gene expression by means of nucleic acidsbiotka.mol.uj.edu.pl/zbm/handouts/2014/JD/09_gene... · Nucleic acids that bind to proteins . 6. Antisense oligonucleotides](https://reader034.fdocuments.in/reader034/viewer/2022050311/5f7310bfcf25fc45f361c864/html5/thumbnails/82.jpg)
Exam
2nd February 2015(Monday)
13 pm, room D107