In-vitro comparison for tolerance to NaCl against callus induction and root- shoot bud formation in...

In-vitro comparison for tolerance to NaCl against callus induction and root-

shoot bud formation in sesame.

P. Chakraborti, S.P. Sinhamahapatra* and A.K. Basu

Department of Seed Science and Technology

*Department of Plant Breeding

Bidhan Chandra Krishi Viswavidyalaya

Mohanpur, Nadia, West Bengal, India

Materials and MethodsNine genotypes – R-9, B-14, B-9, B-

67, T-12, RT-4, IDP-51, IET-2 and HT-1 – primarily, screened from 22 against salt tolerance through germination and survivality. NaCl conc. Were – 0.5% (9.3 dSm-1), 0.75% 13.5 dSm-1), 1.00% (17.4 dSm-1) and 1.25 (20.7 dSm-1). Different explants were placed in MS and B-5 medium with varying conc. of hormones. Explants producing good callus and also multiple shoot bud and root at a particular hormone conc. Were again studied in the same condition supplemented with 0.1%, 0.2% and 0.3% NaCl salt.

Hormone

Conc.

Responsive portion

Types of response Resp-onsive % of the

tube

Colour of the callus

Survival period (days)

Character of the

responsive portion

1 Petiole Medium growth of callus

18 Brownish 17-20 Very soft callus

1 + S -- No response -- -- -- --

2 Plant tip, stem & root

Medium growth of callus

28 Yellowish 19-22 Soft, nodular callus

2 + S Stem & root

Very poor growth of callus

7 Brownish 7-10 Soft callus

3 Plant tip Medium type in performance

24 Yellowish 20-23 Compact, nodular callus

3 + S -- No response -- -- -- --

4 Hypocotyl, Epicotyl & plant tip

Good growth of callus

38 Yellowish white

27-30 Compact, nodular callus

4 + S (Very poor performance) -- -- -- --

5 Hypocotyl, Epicotyl & Plant tip

Good growth of callus

55 Yellowish white

30-38 Compact, nodular callus; root formation after 25-28 days

5 + S Same Poor growth in salty medium

13 Yellowish white

12-13 Soft callus



29 Yellowish 22-28 Soft callus

6 + S Same Poor growth of callus

9 Brownish 8-11 Very soft callus

Response of different explants in MS medium supplemented with different hormones and salinity levels

1 2 3 4 5 6 7


Medium type of initiation of

callus

44 Yellowish 30-32 Compact callus

7 + S Same Decrease growth of callus in

increased salty condition

14 Brownish 12-14 Soft callus

8 Hypocotyl, Epicotyl & Cotyledon

Good initiation of callus followed

by initiation of multiple shoot

buds

65 Creamish white with greenish

tint

30-35 Compact callus, poor

growth of shoot buds

8 + S Same Growth of callus is poor


9 Hypocotyl, Epicotyl & Cotyledon

Good initiation of callus followed by multiple shoot

buds

61 Creamish white

30-33 Compact callus, poor


9 + S Same Medium to poor growth of callus

19 Yellowish 9-13 Soft, nodular callus

10 Hypocotyl, Epicotyl &

Cotyledon & germinating

seeds

Good initiation of callus. Shoot

bud initiated form plumule tip of germinating

seeds


tint

30-38 but

shoot buds

upto 40 days

Compact callus, poor


10 + S Hypocotyl, Epicotyl & Cotyledon

Poor growth of callus

18 Brownish white

10-12 Soft callus

10 Hypocotyl, Epicotyl &

Cotyledon & germinating

seeds

Good start of callus. Shoot bud

initiated from plumule tip of

the germination seeds


tint

30-39 but

shoot buds

upto 40 days

Compact callus, shoot buds initiated

10 + S Hypocotyl, Epicotyl & Cotyledon

Medium to poor initiation of callus with increase in

salinity condition


Hormone

Conc.

Responsive portion

Types of response Resp-onsive % of the

tube

Colour of the callus

Survival period (days)

Character of the responsive

portion

1 Stem, root plant tip


18 Yellowish 18-22 Very soft, nodular callus

1 + S No response

--- --- --- --- ---

2 Stem, root and plant

tip


32 Yellowish 23-28 Soft, nodular

callus, root formation at later stage

2 + S Plant tip Only initiation of callus at lower

conc.

10 Brownish 8-11 --

3 Plant tip Medium growth of callus

30 Yellowish 20-23 Compact, nodular callus

3 + S No response

--- --- --- --- ---

4 Plant tip & Petiole


39 Yellowish 26-33 Nodular callus, root formation at later stage

4 + S Plant tip & Petiole

Poor growth 21 Brownish 10-11 Soft nodular callus

5 Plant tip, Petiole & cotyledon


65 Creamish white

28-34 Compact callus

5 + S Plant tip, Petiole & cotyledon

Poor growth 22 Yellowish 8-12 Compact callus



51 Creamish white



Poor growth 24 Yellowish 10-12 Compact callus

Response of different explants in B-5 medium supplemented with different hormones and salinity levels

1 2 3 4 5 6 7


Good initiation of callus followed by

multiple shoot buds initiation

63 Creamish with

greenish tint

27-41 Compact callus, poor growth of multiple

shoot buds


Medium growth at lower conc.

23 Yellowish white

8-14 Compact callus

8 Plant tip petiole,

cotyledon & germinating

seeds

Good initiation of callus followed by multiple shoot bud

formation at growing plumule tip of germinating

seeds


tint

32-40 Compact callus,

medium growth of multiple

shoot buds

8 + S Plant tip petiole,


seeds but without

germinating seeds

Medium growth of callus at lower

conc.

22 Yellowish white

9-16 Compact callus

9 Plant tip petiole,


seeds

Good initiation of callus & plumule tip of germinating

seeds produced multiple shoot bud


tint

35-41 Compact callus, good development of multiple shoot buds

9 + S Petiole, cotyledon &

plant tip

Medium growth of callus at lower

conc.

22 Yellowish brown

12-14 Compact callus, shoot

buds initiated

10 Plant tip, petiole & cotyledon

Good initiation of callus but not

continued


tint


10 + S Same Medium type of initiation of callus

at lower conc.

20 Yellowish Brown

10-11 Soft callus

1 2 3 4 5 6 7

11 Plant tip, petiole,

cotyledon and germinating

seeds

Good initiation of callus and plumule tip of germinating

seeds produced multiple shoot bud


tint

37-43 Compact callus, good development of multiple shoot buds

11 + S Plant tip, petiole,

cotyledon and germinating

seeds

Moderately callus initiation at lower

conc

23 Brownish 9-12 Compact callus, shoot

buds initiation

Conclusion

• White seeded genotypes responded well for multiple shoot bud.

• Kinetin and BA was much active than 2,4-D.

• Callus growth and multiple shoot bud formation were restricted with increase in salinity.

In-vitro comparison for tolerance to NaCl against callus induction and root- shoot bud formation in...

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