IMPROVING PROFITABILITY OFPOULTRY PRODUCTION THROUGH

PRECISION NUTRITION

BART HILLEN, DANISCO ANIMAL NUTRITION

Title Only

DUPONT 2016 SEGMENT NET SALES

2

Agriculture

39%Nutrition &

Health

13%Performance

Materials

21%Protection Solutions

12%Electronics &Communicatio

ns

8%Industrial

Biosciences

6%

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PROTEIN NEEDS ARE INCREASING

3

17%

101%

-4%

21% 20%

40%

1 2 3 4 5 6-4%

Increasing protein demand needs to be

met with more effective production

o Africa’s population to double by 2050

from approx. 1.3BB to approx. 2.5BB

o Africa is expected to be the highest

growing region in the world 2017-2050

o By 2050, Africa will be the 2nd most

populous region in the world

World Population Forecasted change from 2017 to 2050

Sou

rce:

Uni

ted

Nat

ions

, “W

orld

Pop

ulat

ion

Pro

spec

ts –

Key

Fin

ding

s &

Adv

ance

Tab

les”

201

7 R

evis

ion

Asia Africa N. America OceaniaEurope L. America &

Caribbean

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CHALLENGES POULTRY PRODUCTION 2017

Bas

ed o

n R

auw

et a

l., 1

998

and

Ros

s in

fo

*Amount of feed required for 1Kg weight gain

Improvement in Slaughter Weight vs FCR*

1 9 7 01 9 6 0 1 9 8 0 1 9 9 0 2 0 1 1

2.50 Kg 2.51 Kg 2.04 Kg 1.92 Kg 1.72 Kg

1.68 Kg1.82 Kg

1.98 Kg2.19 Kg

2.77 Kg

4

Title Only

Reduced performance +

mortality

O

Production frontier

Meat

(kg/flock)

Feed, labor, other inputs ($)

Commercial performance

Value

Potential

STILL GENETIC POTENTIAL TO BE CAPTURED

5

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OPPORTUNITY TO IMPACT UNDIGESTED NUTRIENTS TO IMPROVE EFFICIENCY

84,7

96,9

84,383,9

97,8

80,7

83,9

96,7

84,483,6

97,8

82

Protein Starch Fat

Corn

Wheat

Corn+HFI

Wheat + HFI

Rom

ero

et a

l, 20

14, H

FI –

high

fibe

r in

gred

ient

s: c

anol

a, c

DD

GS

6

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WHAT ARE ENZYMES?

SUBSTRATE

ENZYME

ENZYME – SUBSTRATE

COMPLEX

Active Site

Active Site

Product A

Product B

Reaction occurs

releasing products

A and B

Enzyme

surviving

reaction

7

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PHYTIC ACID = INOSITOL PHOSPHOROUS 6 (IP6)

8

Releasing phosphorus from phytate

Myo-inositol hexakisphoshate (phytate) Myo-inositol

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PHYTATE ALSO KNOWN AS IP6 IS THE NATURAL STORE OF PHOSPHORUS IN PLANTS BUT THE CONTENT IS VARIABLE

9

Min

Mean

Max

(PP)

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METHODOLOGY: DIRECT MEASUREMENT OF P RELEASE

Method used by DuPont: Meta- analysis of trial data: more than 10 trials

10

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PHYTATE HAS A NEGATIVE EFFECT ON BROILERS PERFORMANCE

Liu

et a

l.,

20

08

1,40

1,45

1,50

1,55

1,60

FCR, 0-28 d

900

920

940

960

980

1000

BWG, 0-28 d

b

a a

b

11

PP= Phytate P

0.20% PP 0.42% PP 0.20% PP 0.42% PP

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PHYTATE BINDS WITH PROTEIN AT ACID pH

Binds with protein (Wise 1983) Chelates with calcium and trace-elements (Wise 1983)

pH 4.5

Acidic pH Basic pH

1

32

56

OH

P OOH

O

H

OH

P OOH

O

H

H

OH

P OOH

O

H

O H

PO O H

O

OH

P OOH

O

H

H

O

H

P OOH

O

4

Phytate reduces the availability of

• protein,

• calcium,

• trace-elements

• and possibly other nutrients such as

starch

12

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PHYTASE APPLICATION

1 9 9 01 9 8 0 2 0 0 0 2 0 1 0 2 0 1 5

Aspergillus-3 Peniophore

Lyeii

Citrobacter

braakil

E. Coli-6 &

E. Coli

(Trichoderma

reesel)

Buttlauxella spp

(Trichoderma

reesel)

Source

13

DoseSubstrate

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PHYTASES DO NOT BEHAVE THE SAME WAY

Assay run for 30 minutes at 37°C, using 5.1 mM Na-phytate as a substrate and 0.02

FTU/ml

DuP

ont L

abor

ator

y, 2

012

0

20

40

60

80

100

120

140

160

180

200

2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7

%A

ctiv

ivy

rela

tive

to

pH

5.5

pH

Axtra PHY Citrobacter phytase E.Coli phytase Peniophora phytase

Axtra®PHY

E.Coli phytaseP.lycii Phytase

Citrobacter Phytase

All phytases are

standardized at

pH 5.5

Gizzard Crop & Small Intestine

Proventriculus

% Activity

relative

to pH 5.5

14

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RETENTION TIME IS LIMITED, ESPECIALLY IN THE PROVENTRICULUS AND GIZZARD, THE MAIN LOCATION FOR PHYTATE HYDROLYSIS

*Short chain fatty acids

12-24h for small

particles

SMALL INTESTINE

60 - 120 mins

pH 5-6.5

PROVENTRICULUS

& GIZZARD

60 - 90 mins

pH 2-4

COLON

30 – 60 mins

pH 7

Hydrolysis

CROPUp to 120 mins

pH 5-6

Hydrolysis and

absorption

15

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FEED INGREDIENTS CHANGE CONSTANTLY,OUR PRODUCTS ARE DESIGN TO KEEP YOU AHEAD

16

Cost

Feed & RM

Quality

Animal

Performance

OPTIMIZING THE OPPORTUNITY FOR FEED COST-SAVING FROM ENZYMES VS. THE RISK OF INCURRING NUTRITIONAL DEFICIENCY

NUTRITIONAL

DEFICIENCY

Phytase Research & Science

behind matrix values decreases risk17

COST SAVINGSFROM ENZYMES

OPTIMIZEOPPORTUNITY HEALTHY

Risk – the Right

Balance

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WHAT ABOUT OTHER SUBSTRATES THAN PHYTATE?

PhytateXylanase

ß-Glucanase

Fibres Protein StarchBacillus subtilis

Bacillus licheniformisAmylase

A.niger

C.braachi

E.coli

Citrobacter spp

Buttiauxella spp

18

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CELLULOSE, HEMICELLULOSE, LIGNIN –MOST IMPORTANT CHARACTERISTICS OF CELL WALLS

Plant cellsLayered mesh of

microfibrils in

plant cell wall

Cell wall

19

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CORN MORPHOLOGY IS IMPORTANT TO DEGREE OF STARCH-PROTEIN BINDING, DEGREE OF STARCH DIGESTION AND RESPONSIVENESS TO ENZYMES

Vitreous Endosperm Floury Endosperm

Starch

Granule

Protein

Matrix

Publis

hed w

ith p

erm

issio

n:

Copyright (2

003)

National A

cadem

y o

f S

cie

nces, U

SA

20

Title Only

21

CORN STARCH DIGESTIBILITY IS ALSO VARIABLE

20,0

30,0

40,0

50,0

60,0

70,0

80,0

1 6 11 16 21 26 31 36 41 46 51 56 61 66 71 76 81 86 91 96 101

106

111

116

121

126

131

136

141

146

151

156

161

166

171

176

181

186

191

196

201

206

211

216

221

Starch %

In vitro starch

digestibility %

56%

25%

Dan

isco

Cor

n H

arve

st R

epor

t 220

sam

ples

73%

69%

21

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AGENDA

WHO IS

DUPONT?

01

POULTRY

PRODUCTION

CHALLENGES

02

ENZYMES

AND THEIR

ROLE IN

MODERN

DAY

BROILER

NUTRITION

03

SUMMARY

04

Title Only

1.52b

1.59a

1.56ab

1.58a1.58a

1.52b

1,48

1,50

1,52

1,54

1,56

1,58

1,60

1 2 3 4 5 6

COMBINATIONS INCREASE CONSISTENCY, PARTICULARLY ON TOP OF HIGH PHYTASE DOSES

Am

erah

et a

l., 2

016

All diets contained 1000 FTU Buttiauxella sp. phytase /kg

23

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SUMMARY

o Enzymes help increasing feed digestibility

o Modern birds yield higher performance

o RM prices increase, demanding more creative use of raw materials

o Variability in raw materials results in more difficult substrates

o Axtra® PHY is fastest acting phytase at low pH

o Reduce risk with reliable matrix values and services

o Superior enzyme combination for corn based diets: Axtra® XAP

24

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