ﺺّﺨﻠﻤﻟا · 2010-11-04 · Rhizopus, Mucor ScopulariopsisPenicillium Fusarium...
Transcript of ﺺّﺨﻠﻤﻟا · 2010-11-04 · Rhizopus, Mucor ScopulariopsisPenicillium Fusarium...
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Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series Vol. (30) No. (2) 2008
)٥/٣/٢٠٠٨ قبل للنشر في . ٢٠٠٨ / ١ / ٢تاریخ اإلیداع (
� الملّخص �
1611/PDA,MSA
PDA
209.7X105Cladosporium Aspergillus
AlternariaPenicillium Rhizopus Scopulariopsis Absidia Mucor
100AspergillusCladosporium
vuillAspergillus fumigatus
9.614.6
B1,B2,G1,G2
16 PPB/
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Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series Vol. (30) No. (2) 2008
Detecting Contamination of Soybean Meal by Storage Fungi and Aflatoxins
Dr. Sabah Almaghribi* Dr. Mahmud Hasn**
Noha Alio***
(Received 2 / 1 / 2008. Accepted 5/3/٢٠٠٨)
� ABSTRACT �
16 species have been obtained from 11 samples of local and imported soybean meal. The isolation is on PDA, MSA media. The average number of fungal colonies is higher on PDA, where it notches 209.7x10 5 colony/g. The most important isolated species are: Cladosporium, Aspergillus, Alternaria, Penicillium, Rhizopus Scopulariopsis, Absidia, and Mucor. The contamination percentage of the samples concerned is 100% with Aspergillus, Cladosporium, and the most prevalent species is Aspergillus fumigatus vuill. The moisture content of samples ranges between 9.6 and 14.6% and does not show any obvious relationship with the Aflatoxins concentration. All samples are contaminated with Aflatoxins, and the highest percentage is 16 ppb. Keywords: Aflatoxins, Fungi, soybean meal.
* Professor, Department of plant protection, Faculty of Agriculture, Tishreen University, Lattakia,
Syria ** professor, Department of plant protection, Faculty of Agriculture, Tishreen University, Lattakia,
Syria. *** Postgraduate Student, Dep. Plant Protection, Faculty of Agriculture, Tishreen University, Lattakia,
Syria.
Tishreen University Journal. Bio. Sciences Series ٢٠٠٨) 2(العدد ) ٣٠( العلوم البیولوجیة المجلد مجلة جامعة تشرین
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2005
100120
2005
1997
42
45
2005
Krysinska & Dutkiewicz, 2000
AspergillusFusariumPenicillium ScopulariopsisMucorRhizopus,
CladosporiumAspergillus FusariumPenicillium
Okoli et) al.,2007
Aspergillus Aspergillus fumigatus
Aspergillosis)
24482005
A. flavus A. parasiticus
Aflatoxins 2003
Oliveira et al.,2006
Bennett & Klich, 2003
Fusarium87
300Dalcero et al.,1997
Penicillium
MucorAbsidiaRhizopus
2000
١٩٢
Chelkowski (1991)
CFU1x 105Sweeney Dobson
(1998)
B1,B2,G1,G2
20062007
11
Denver4105
5
P.D.A
1990
M.S.A
Maghribi,1985 ;2000
2018030
1012 18102
101105
115
45Kacaniova,2003
25710
Tishreen University Journal. Bio. Sciences Series ٢٠٠٨) 2(العدد ) ٣٠( العلوم البیولوجیة المجلد مجلة جامعة تشرین
١٩٣
Onion et al;1981
Kacaniova,2003
Onion et al., 1981; Nelson et al., 1983
B1,B2,G1,G2
Aflatest minicolumnVicam Fluorometer
050 PPBVicam,1997
11PDAMSA
1
PDAMSA
PDA209.7X1051990
1
PDAMSA
1333.0x10 5666.0x10 5
4366.0x10 5 266.0x10 5
566.0x10 5 0.1x10 5
7 333.0x10 5 66.0x10 5
8 333.0x10 5 0.1x10 5
9 233.0x10 5 1.2x10 5
3 166.0x10 5333.0x10 5
2 0.2x10 5 133.0x10 5
6243.0x10 5 2.1x10 5
10 233.0x10 5 0.1x10 5
11 1.0x10 52.1x10 5
209.7X10 5 133.6X10 5
L.S.D5% 76.1
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2Sweeney Dobson1998
3
Aspergillus fumigatus
37Bennett & Klich,
20032000
2
Aspergillus 11100
Penicillium 872.72
Cladosporium 11100
Mucor 654.54
Rhizopus 872.72
Absidia 872.72
Alternaria 9 81.81
Fusarium 4 36.36
Scopulariopsis 8 72.72
Cephalosporium 654.54
Nigrospora 1 9.09
Mycelia sterilia 1 9.09
Pythium1 9.09
Phytophthora1 9.09
Mortierella1 9.09
Syncephastrum1 9.09
8548.29188
1623
Aspergillus 100
Okoli et al.,2007)Aspergillus
fumigatus A. glaucus A. candidus,A. flavus
Kore, 1996Cladosporium
CladosporinEmodinDehoog et al.,2000
Tishreen University Journal. Bio. Sciences Series ٢٠٠٨) 2(العدد ) ٣٠( العلوم البیولوجیة المجلد مجلة جامعة تشرین
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Rhizopus, Penicillium, Absidia, Scopulariopsis
Kacaniova,2003 Absidia
( Knudtson & Kirkbride,1992 Penicillium
Ochratoxins
Bennett & Klich,
2003;1991Rhizopus
ToxoflavenRhizopteren2000
Scopulariopsis
Esterella et al.,2003
Issakainen et al.,2007
Fusarium36.36
Zearalenone
Fumonisin Fusarin TrichothecenesT-
2ToxinPark et al.,1999
3
Penicillium spp. Link ex S.F.Gray
Cladosporium spp. Link ex Fries
Mucor racemosus Fresenius Mucor sp. Micheili ex Fries
Rhizopus stolonifer (Ehrenberg ex Fr) LinderRhizopus sp. . Ehrenberg ex Corda
Absidia ramosae(Lindt) Lendner Absidia sp.Van Tighem
Aspergillus fumigatus vuillA. flavus Link ex Fries
A. niger V.Tiegh A. candidus Link A. terreus Thom A. tamarii Kita
A. ochraceus Wilhelm A. versicolor Vuill
A. glaucus Link ex Fries A. sydowii
Mycelia sterilia Alternaria spp.Nees ex Wallroth
Pythium sp.F Fusarium moniliforme Sheldonusarium spp. Link ex Fries
Phytophthora sp.Scopulariopsis brevicaulis (Sacc)Bainier
Morterila ramanniana Moler(Linnemann)Cephalosporium sp. Link ex Fries
Syncephastrum racemosum(Con)SchroterNigrospora sp. Berk &Broome
B1,B2,G1,G2
Aflatest minicolumnVicam
Fluoromete
١٩٦
Gupta and Venki 1975
24A. flavus
4A. flavus
4
2680
10PPB
20PPB2002
4
B1,B2,G1,G2PPB
9.614.6
12(Okoli et al.,
2006)
Tishreen University Journal. Bio. Sciences Series ٢٠٠٨) 2(العدد ) ٣٠( العلوم البیولوجیة المجلد مجلة جامعة تشرین
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1990599
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1997929930
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2003253258
4
2005
413
52000
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1991179
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2005371
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2005283284391392
9
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2002
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