Impact of food habit on body elements level and health
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Transcript of Impact of food habit on body elements level and health
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IMPACT OF FOOD HABIT ON BODY ELEMENTS LEVEL AND HEALTH
Dr. G. NAGARAJ
Lab. Asst. in Zoology
ZOOLOGY SECTIONREGIONAL INSTITUTE OF EDUCATION (NCERT)
MYSORE e-mail: [email protected]
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Background • Every food item is a source for multi-
element exposure
• Food habit may have association with changes in
body element levels
• Such altered status of body elements may leads to
health disorders
• It is needed to assess concentration of elements in
subjects with chronic health hazard
• Such assessment is done by biological monitoring
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Objectives of the study
To find out the influence of food habit on body levels of trace elements and in turn it’s effect on physical health of Mysore subjects.
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MATERIALS AND METHODS
1. Subjects: The residents of Mysore city & surrounding villages (M:100 & F:76)
2. Samples: Teeth, blood, urine, scalp hair & fingernail3. Sampling places: Teeth: JSS dental college, Mysore Blood, scalp hair, fingernails and urine: Kamakshi,
Bassappa Memorial and Vickram Hospitals, Mysore.
4. Questionnaire: personal and sample characters were collected
5. Processing of samples:Washing: T, F - DDW, H, N, - DDW & Acetone-wit magnetic stirrerDrying: Hot air oven - 50C - 24hrDigestion: HNO3 & HClO4 (4:1), Kjeldhal apparatus – 80’C - 8 hr Make up to 10 ml with DDW and stored in refrigerator at 4’C
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Element Analysis:
Sample Element Instrument Place
TeethCr, Cu, Fe, Ni, Pb, Zn
AAS 6 VARIO
AAS-GBC 902
CES- Anna Univ, Chennai.
SES-JNU- New Delhi.
Blood, urine, hair,
fingernail,
Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Zn
ICP-AES - JY-IYON-2002–2 DEE - SJCE,
MysoreQuality control studies: Certified Reference Material (CRM) i.e. human hair powder supplied by the National Institute of Environmental studies (NIES), Japan, (NIES-CRM) was analysed Anna University, Chennai Jawaharlal Nehru University (JNU), New Delhi.
Statistical analysis: Mean, Standard Deviation, Student ‘t’ test.
Mean elements concentration for food habit and blood sugar level were computed in each sample & given in following tables;
ResultTable 1. Comparison of element levels of blood
Note: Significance: * -P 0.05, N – Number of samples, SD- Standard Deviation.
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Table 2. Comparison of element levels of teeth
Note: Significance: * -P 0.05, N – Number of samples, SD- Standard Deviation
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Table 3. Comparison of element levels of urine
Note: Significance: **-P 0.01, N – Number of samples, SD- Standard Deviation.
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Table 4. Comparison of element levels of scalp hair
Note: Significance: * -P 0.05, N – Number of samples, SD- Standard Deviation
Table 5. Comparison of element levels of fingernail
Note: Significance: **-P 0.01, N – Number of samples, SD- Standard Deviation.
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FINDINGS
1.Rural vegetarian had lower Cd-B & Pb-B than non-veg.
2.Rural diabetics had higher Co-H & Fe-H than non-diabetics.
3.Urban diabetics showed lower Zn-FN, & higher Cd-FN,
Cr-FN, Cu-FN, Mn-FN, Ni-FN, Pb-FN & Pb-T.
4.Lower Zn & Higher Pb are due to food habit & environment;
is one of the casual factors related to diabetes.
5.Positive correlation exists between elements & diabetes.
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CONCLUSION
Food habit affects/changes the elements level and causes elements imbalance leading to altered element metabolism, which may be one of the causal factor for diabetes.
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THANK YOU