iGEM Fusion

Science, Technology, Engineering, and Math all rolled up into iGEM Ung.igem.org/High_School_Division iGEM Fusion


iGEM Fusion. Science, Technology, Engineering, and Math all rolled up into iGEM Ung.igem.org/ High_School_Division. Synthetic Biology. Foundation Science for iGEM Synthetic Biology take molecular genetics and apply engineering principles: Standardization: - PowerPoint PPT Presentation

Transcript of iGEM Fusion

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Science, Technology, Engineering, and Math all rolled up into iGEM


iGEM Fusion

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Foundation Science for iGEMSynthetic Biology take molecular genetics and apply engineering principles:

Standardization:Development of systems that are standards in the biological worldex. Restriction enzyme systems, promoters/translational unit systemsDecoupling:Separating a complex system into simple systems that work togetherex. Building a fragrance free chassis, building tolerance to salt water, sense arsenic, and fragrance system. When they are put together they can make a bacteria that can tolerate salt water, sense arsenic and release a fragrance in the presence of arsenic.Abstraction:Systems can be thought of in a hierarchy in two formsWork in one level and disregard the other levelsAllow for the exchange between levelsex. Relationship between RBS and ORF

Synthetic Biology

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Teams design, engineer, and build a biological machine from standard partsTeam structure:All students should have beginning biologySelect students that are willing to work after schoolMay want students that have a computer backgroundTimelineFlexible schedule dependent on the studentsAfterschool activityCorrelate with curriculum in a classRegistration is from Mid August to Mid Novemberregister at http://ung.igem.org/Main_PageTeam abstract are due in May with registration to the jamboree.

Declare a track for the jamboree. Teams will be judged based on the track chosen.

Environmental, Health and Medicine, Informational Processing, Food and energy, Foundational Advance, Manufacturing, New Application, Software Tools

Jamboree is last Saturday in JuneBudgetIf you have the equipment, $300-$1,000 to do the lab work

iGEM: International Genetically Engineered Machine

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Project idea:Project brain storming session relevant to a need in society

Ethics of synthetic biology: Just because we can does not mean we do

Pick a Biological system Research the host: based on the complexity and mentors available to your team

Make sure that you have competent cells to work with. Most cells that come from kits are considered competent. You can look on the suppliers website to find out.

Research existing biological circuitsTeam members research current parts that my be available for their system. These parts can be from the host or form the registry.

Design the new circuit to manipulate the hostTeam members design the plasmid’s circuit to allow the host to become the machine. This can be a multiple step system where students set goals to achieve during the building session. Students research plasmid and part design to make their robot. They research current parts and systems to

help with the designing.

Community outreach program:Team members talk about their project to the community .

Hook activitiessmelly bacteria or photogenic bacteria

Footsteps of an iGEMer: Fall Semester

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Building of the machine:Teams build the machine using partsInsertion into a plasmidTransformation into the host cell

Standardization of resultsMathematically compute what the results mean to the userex. Level of florescence to concentration of promoter

Celebration of successJamboree

Teams get together to display their results at the poster session. Then teams do a 20 minute presentation to judges.October

Teams can be volunteers for the Americas regional in IndianapolisTeams present their posters during the poster session at the Americas


Footsteps of an iGEMer: Spring Semester

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Search for PartsNatural DNA

Parts from the Registry

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iGEM parts that are received after registration

Extraction of the parts form the genomeUsing primers ordered from a vendor

Write a lab that has the part and request that partIn the research of the part, students will read about

labs performing testing. Students can contact the lab and ask for a sample.

Acquire the Parts

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Restriction Enzymes

3 A Assembly Method

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Primers and homologiesC:\Users\William Schini\Videos\The Gibson Assembly Song.mp4

Gibson Assembly

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Community outreach: 2012 each team will be asked to develop and implement a community outreach program. Students can talk with local groups about their project. Develop activities and perform them with middle school students. Ethics of Synthetic Biology (GMO):Team leaders can discuss the ethics of GMO’s and understand the uses of GMO’s

Create new parts for submission into the registry


Create new plasmid backboneSubmit work for publishing

Other avenues

Benefits for the student:Any team competing in the jamboree will be able to

present their poster at the collegiate regional. Potentially to the final jamboree.

Students get to meet with leading scientists in the field of molecular genetics, synthetic biology, biology, math, and engineering.

Connections developed through contact with mentors and scientists.

Colleges do notice iGEM on applications, especially schools of medicine.