Thermo ScientificHypersil GOLD HPLC Columns

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Outstanding peak shape for your separations

Designed for improved chromatography, Thermo Scientific™ Hypersil GOLD™

columns are the culmination of over 35 years of experience in the product

development and manufacturing of HPLC media and columns. The range

and capabilities of this state-of-the-art family of columns, with numerous

chemistries and a range of particle sizes and hardware formats meet the

challenges of modern chromatography.

The highly pure Hypersil GOLD silica is manufactured, bonded and packed

in ISO 9001:2008 accredited facilities, operating under strict protocols using

robust procedures and extensive quality control testing. The manufacturing

and bonding process creates an even surface with fewer silanols leading

to reduced secondary interactions. This ensures consistent performance,

column after column.

Hypersil GOLD HPLC columns are available in 12 different chemistries to optimize separations and maximize productivity. The extensive range of Hypersil GOLD columns offers chromatographers outstanding peak shape for reversed phase, ion exchange, HILIC or normal phase chromatography. With all 12 phases being available with 1.9 μm particle size, Hypersil GOLD columns offers chromatographers flexibility in choosing the correct column, whether they are using conventional or ultra-high pressure LC systems.

Thermo Scientific Hypersil GOLD Columns

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Hypersil GOLD ColumnsOutstanding Peak Shape Using Generic Gradients with C18 Selectivity 2–7Hypersil GOLD C8 ColumnsEnhanced Resolution, Efficiency, Sensitivity and Speed 8–9Hypersil GOLD C4 ColumnsLow Hydrophobicity Columns for Less Retention 10Hypersil GOLD aQ ColumnsEnhanced Retention and Resolution of Polar Analytes 11–12Hypersil GOLD PFP ColumnsUnique Selectivity with Perfluorinated Columns 13–14Hypersil GOLD CN ColumnsCyano Columns for Reversed and Normal Phase Separations 15–16Hypersil GOLD Phenyl ColumnsExcellent Retention and Unique Selectivity for Aromatic Analytes 17–18Hypersil GOLD Amino ColumnsHighly Versatile Aminopropyl Stationary Phase 19Hypersil GOLD AX ColumnsSeparation of Anionic Species and Polar Molecules 20Hypersil GOLD SAX ColumnsQuarternary Amine Strong Anion Exchange Column, Designed for Aqueous Mobile Phase 21Hypersil GOLD Silica ColumnsExcellent Peak Shape in Normal Phase Chromatography 22Hypersil GOLD HILIC ColumnsEnhanced Retention of Polar and Hydrophilic Analytes 23Hypersil GOLD 1.9 μmSmall Particles to Improve Speed and Efficiency 24–26Hardware SolutionsHardware Solutions for High Throughput Screening, Capillary and Preparative Chromatography 27Column ProtectionExtend Column Lifetime and Improve Performance 28

Ordering Information 29–32

Improved Selectivity, Resolution and Productivity

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Hypersil GOLD columns offer improved peak shape, even for basic analytes.

Enhanced Resolution

Robust assay development requires a clear definition of resolution expectations. Narrow symmetrical chromatographic peaks ensure that optimum resolution is achieved. Obtaining narrow peak widths is especially challenging for basic pharmaceutical compounds. The reduced silanol activity on Hypersil GOLD columns reduces tailing for basic analytes, thus improving resolution.

Hypersil GOLD columns are based on improved, highly pure silica and a novel proprietary derivatization and endcapping procedure using alkyl chain chemistry. This gives:

• Significant reduction in peak tailing while retaining C18 (USP L1) selectivity• Excellent resolution, efficiency and sensitivity• Confidence in the accuracy and quality of analytical data

Competitor C18 column

Hypersil GOLD columns provide excellent resolution between critical pairs, aiding separation of closely related species.

Hypersil GOLD Columns Outstanding peak shape using generic gradients with C18 selectivity

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Improved Sensitivity

Outstanding peak shape results in greater sensitivity. When peaks exhibit tailing, peak height is reduced, therefore compromising the sensitivity of the analysis. The highly symmetrical peaks provided by Hypersil GOLD columns enhance peak height and allow for optimised peak integration calculations. This can be particularly critical when low concentrations of an analyte are present, for example in an impurity assay.

Reproducibility

Our Hypersil GOLD columns are exceptionally reproducible forreliable chromatography, column after column. This allows theuser to be confident that assays developed with Hypersil GOLDcolumns will be robust and stable for the life of the assay,making them an ideal choice for new method development.

pH Stability

Our Hypersil GOLD columns are well suited to extended pHapplications and have been shown to produce robust assaysat high pH. At low pH, excellent column stability andreproducibility are illustrated over 1500 injections at pH 1.8.

Stability of Hypersil GOLD columns at low pH. No loss of retention after 28 L of mobile phase in 19.5 days of analysis.

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Column: Hypersil GOLD , 5 µm, 150 × 4.6 mm Mobile Phase: A: 0.1% ammonia pH 10.6 B: Methanol + 0.1% ammonia Gradient: 5–100% B in 15 min Flow Rate: 1.0 mL/min Injection Volume: 10 µL Detection: UV @ 254 nm Temperature: 30 °C

Hypersil GOLD Columns

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Column: Hypersil GOLD, 5 µm, 150 × 4.6 mm Mobile Phase: A: 0.1% acetic acid B: Acetonitrile Gradient: 20–70% B in 10 mins Flow Rate: 1 mL/min Detection: UV @ 254 nm Temperature: 25 °C

Column: Hypersil GOLD, 5 µm, 150 × 4.6 mm Mobile Phase: A: 20 mM ammonium formate at pH 3.0 B: Methanol Gradient: Time (min) % B 0 10 5 10 10 70 Flow Rate: 1.5 mL/min Detection: UV @ 270 nm Temperature: 25 °C

Column: Hypersil GOLD, 5 µm, 150 × 4.6 mm Mobile Phase: A: 0.05 M monopotassium phosphate pH 3 B: Acetonitrile Isocratic: 50:50 Flow Rate: 1.25 mL/min Detection: UV @ 220 nm Temperature: 25 °C

Pharmaceutical Cepha antibiotics

Cough/cold formulation

Anaesthetics

1. Cefadroxil 2. Cefaclor 3. Cephalexin 4. Cephradine 5. Cefazolin

1. 4-Amino phenol 2. (chlorpheniramine) maleate 3. Phenylephrine 4. Acetaminophen 5. Saccharin 6. Impurity from 4-Amino phenol 7. 4-Nitro phenol 8. Chlorpheniramine

1. Lidocaine 2. Tetracaine 3. Benzocaine

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Column: Hypersil GOLD, 5 µm, 150 × 4.6 mm Mobile Phase: A: Methanol B: Water Isocratic: 75:25 Flow Rate: 1 mL/min Detection: UV @ 269 nm Temperature: 25 °C

Column: Hypersil GOLD, 3 µm, 150 × 2.1 mm Mobile Phase: A: 25 mM ammonium acetate at pH 5 B: Acetonitrile Gradient: 20–100% B in 10 min Flow Rate: 0.2 mL/min Detection: UV @ 254 nm Temperature: 40 °C

Column: Hypersil GOLD, 5 µm, 150 × 4.6 mm Mobile Phase: A: Water B: Acetonitrile Gradient: 25–70% B in 20 min Flow Rate: 1.5 mL/min Detection: UV @ 220 nm Temperature: 25 °C

Environmental Polycyclic aromatic hydrocarbons

Banned aromatic amines

Endocrine disruptors

1. Naphthalene 2. Fluorene 3. Phenanthrene 4. Anthracene 5. Pyrene 6. Chrysene

1. 2,4-Diaminotoluene 2. 4,4-Oxydianiline 3. o-Toluidine 4. 2-Methoxy-5-methylaniline 5. 2,4,5-Trimethylaniline 6. 4,4'-Methylene-bis (2-chloroaniline) 7. Unknown

1. Desethyl atrazine 2. Estriol 3. Simazine 4. Atrazine 5. Diuron 6. Bisphenol A 7. Estrone

1. Cefadroxil 2. Cefaclor 3. Cephalexin 4. Cephradine 5. Cefazolin

1. 4-Amino phenol 2. (chlorpheniramine) maleate 3. Phenylephrine 4. Acetaminophen 5. Saccharin 6. Impurity from 4-Amino phenol 7. 4-Nitro phenol 8. Chlorpheniramine

1. Lidocaine 2. Tetracaine 3. Benzocaine

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Column: Hypersil GOLD, 5 µm, 150 × 4.6 mm Mobile Phase: A: Water B: Acetonitrile Isocratic: 43:57 Flow Rate: 1 mL/min Detection: UV @ 254 nm Temperature: 25 °C

Column: Hypersil GOLD, 5 µm, 50 × 2.1 mm Mobile Phase: A: 0.1% formic acid B: Acetonitrile + 0.1% formic acid Gradient: 15–80% B in 5 min Flow Rate: 1 mL/min Detection: UV @ 254 nm Temperature: 30 °C

Column: Hypersil GOLD, 5 µm, 150 × 4.6 mm Mobile Phase: A: 0.1% formic acid B: Acetonitrile + 0.1% formic acid Gradient: 30–50% B in 15 min Flow Rate: 1 mL/min Detection: UV @ 254 nm Temperature: 30 °C Concentration: 2.5 ng/µL

Toxicology Testosterones

Chlorpromazine

Tricyclic antidepressants

1. 11-Ketotestosterone 2. 19-Nortestosterone (nandrolone) 3. Testosterone 4. Epitestosterone

1. Chlorpromazine

1. Doxepin 2. Protriptyline 3. Imipramine 4. Nortriptyline 5. Amitriptyline 6. Trimipramine

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Column: Hypersil GOLD, 5 µm, 150 × 4.6 mm Mobile Phase: A: 10 mM ammonium acetate at pH 5.0 B: Methanol Gradient: 30–45% B in 10 min Flow Rate: 1 mL/min Detection: UV @ 230 nm Temperature: 25 °C

Column: Hypersil GOLD, 5 µm, 150 × 4.6 mm Mobile Phase: A: 0.1% formic acid B: Acetonitrile Isocratic: 70:30 Flow Rate: 1 mL/min Detection: UV @ 270 nm Temperature: 40 °C

Column: Hypersil GOLD, 5 µm, 150 × 4.6 mm Mobile Phase: A: Water B: Methanol Isocratic: 5:95 Flow Rate: 1 mL/min Detection: -ESI Temperature: 30 °C

Food Safety Energy drink additives

Coumaric acids

Tocopherols

1. Acesulfame 2. Saccharin 3. Caffeine 4. Benzoic acid 5. Sorbic acid 6. Aspartame

1. Uracil 2. p-Coumaric Acid 3. m-Coumaric Acid 4. o-Coumaric Acid

1. δ-Tocopherol 2. γ-Tocopherol 3. α-Tocopherol

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Similar Selectivity but Less Retention Than C18

Hypersil GOLD C8 media provides similar selectivity to C18 with a predictable elution order, but less retention. This feature isis particularly useful where smaller amounts of hydrophobicity are needed in order to successfully retain compounds ofof interest. Hypersil GOLD C8 columns are recommended for analytes of medium hydrophobicity or when a less hydrophobicphase is required to obtain adequate retention.

Faster Separations

Hypersil GOLD C8 columns can provide improved throughput of analysis over that of a C18 alkyl chain chemistry. Hydrophobicinteractions are reduced, allowing compounds to elute quicker from the column.

• Analytes of medium hydrophobicity• When a less hydrophobic phase is required to obtain adequate retention

Hypersil GOLD C8 Columns Enhanced resolution, efficiency, sensitivity and speed

Excellent Peak Shapes with High Efficiency and Outstanding Sensitivity

Hypersil GOLD C8 columns provide very symmetrical peak shapes while also improving capabilities such as speed of analysis, efficiency and sensitivity.

Hypersil GOLD C8 Columns

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Column: Hypersil GOLD C8, 5 µm, 150 × 4.6 mm Mobile Phase: Methanol Flow Rate: 1.5 mL/min Detection: UV @ 450 nm Temperature: 25 °C

Column: Hypersil GOLD C8, 5 µm, 150 × 4.6 mm Mobile Phase: A: 0.1% formic acid B: Acetonitrile Isocratic: 15:85 Flow Rate: 1 mL/min Detection: UV @ 200 nm Temperature: 25 °C

Column: Hypersil GOLD C8, 5 µm, 150 × 4.6 mm Mobile Phase: A: Water B: Acetonitrile Gradient: Time (min) % B 0 20 15 23 25 75 Flow Rate: 1.5 mL/min Detection: UV @ 240 nm Temperature: 25 °C

Column: Hypersil GOLD C8, 5 µm, 150 × 4.6 mm Mobile Phase: A: Water B: Acetonitrile Gradient: 60–90% B in 10 min; hold 10 min Flow Rate: 1 mL/min Detection: UV @ 254 nm Temperature: 25 °C

Fatty acids

Environmental Triazines and uron herbicides

Phthalates

1. Linolenic Acid 2. Linoleic Acid

1. Simazine 2. Monuron 3. Chlorotoluron 4. Atrazine 5. Diuron 6. Propazine 7. Linuron

1. Dimethyl phthalate 2. Diethyl phthalate 3. Dipropyl phthalate 4. Diisopropyl phthalate 5. Di-n-butyl phthalate 6. Di-n-octyl phthalate

1. Lutein 2. Lycopene 3. β-Carotene

Food Safety β-Carotene

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Lower Hydrophobicity for Faster Separations

Hypersil GOLD C4 columns provide similar selectivity to C18 and C8 columns but with less retention. The shorter chain length and lower hydrophobic character make C4 a particularly useful stationary phase for the retention and separation ofhydrophobic polypeptides and proteins.

• Analytes with high hydrophobicity• When a less hydrophobic phase is required to obtain adequate retention

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Hypersil GOLD C4 Columns Low hydrophobicity columns for less retention

Excellent Peak Shape, Showing High Efficiency and Outstanding Sensitivity

Based on the same highly pure silica, Hypersil GOLD C4 columns deliver excellent peak shape. For high speed, high efficiency separations, Hypersil GOLD C4 columns are available with 1.9 μm particle size.

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Column: Hypersil GOLD C4, 5 μm, 150 × 4.6 mm Mobile Phase: Water/acetonitrile (50:50) Flow Rate: 1.0 mL/min Temperature: 25 °C Detection: 214 nm Injection volume: 10 μL

Column: Hypersil GOLD C4, 1.9 μm, 100 × 2.1 mm Mobile Phase: Water/acetonitrile (20:80) Flow Rate: 0.55 mL/min Temperature: 30 °C Detection: 200 nm Injection volume: 1 μL

Pharmaceutical Parabens

Food safety Fatty Acids

1. Methylparaben 2. Ethylparaben 3. Propylparaben 4. Butylparaben

1. Linolenic acid 2. Linoleic acid

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Retention and Resolution of Polar Analytes

Because Hypersil GOLD aQ columns are packed with a polar endcapped C18 phase, they offer superior retention of polarcompounds. Dispersive interactions are the primary mechanism of retention with alkyl chain bonded phases. The polarfunctional group used to endcap Hypersil GOLD aQ media provides an additional controlled interaction mechanism by which polar compounds can be retained and resolved. The resulting optimized peak shape provides excellent resolution sensitivity and efficiency, making Hypersil GOLD aQ columns ideal for the quantitative analysis of trace levels of polar analytes.

Polar Endcapped C18 Stationary Phase for Alternative Selectivity

The additional interaction mechanism often provides selectivity differences over the traditional alkyl chain chemistries, andoffers a solution for the separation of polar compounds which exhibit insufficient retention on pure alkyl chain phases undertypical reversed phase mobile phase conditions.

• Analysis of water soluble vitamins and organic acids• Use with highly aqueous mobile phase

Ideal for Highly Aqueous Mobile Phases

The wettability of reversed phase media can be increased by the introduction of polar functional groups. The polar endcapping of Hypersil GOLD aQ media also makes it usable in 100% aqueous mobile phases without the risk of loss of performance or poor stability.

Excellent Peak Shapes

Hypersil GOLD aQ silica, ensures optimized peak shape, resolution, sensitivity and efficiency. Hypersil GOLD aQ columns provide only controlled secondary interactions to ensure excellent peak shape for all analyte types, making them ideal for the quantitative analysis of trace levels of polar analytes.

Hypersil GOLD aQ Columns Enhanced retention and resolution of polar analytes

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Hypersil GOLD aQ Columns

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Column: Hypersil GOLD aQ, 5 µm, 150 × 4.6 mm Mobile Phase: A: 50 mM monopotassium phosphate pH 3.5 B: Methanol Gradient: 0–100% B in 15 min Flow Rate: 1 mL/min Detection: UV @ 205 nm

Column: Hypersil GOLD aQ, 5 µm, 150 × 4.6 mm Mobile Phase: A: 0.1% formic acid B: Acetonitrile + 0.1% formic acid Gradient: 1 0–100% B in 15 min Flow Rate: 1.0 mL/min Detection: UV @ 270 nm Temperature: 30 °C

Column: Hypersil GOLD aQ, 5 µm, 150 × 4.6 mm Mobile Phase: A: 50 mM monosodium phosphate pH 2.5 B: Methanol Gradient: 1–100% B in 10 min Flow Rate: 1 mL/min Detection: UV @ 254 nm Temperature: 30 °C

Column: Hypersil GOLD, 5 µm, 150 × 4.6 mm Mobile Phase: A: 0.1% tetrahydrofuran + 0.015% triethylamine in water B: 0.1% tetrahydrofuran + 0.015% triethylamine in acetonitrile Gradient: Time (min) % B 0 17 2 20 7 35 20 35 Flow Rate: 1 mL/min Detection: UV @ 269 nm Temperature: 25 °C

Pharmaceutical Sulfonamides

Xanthines

Biochemical PTH amino acids

1. Sulfaguanidine 2. Sulfanilamide 3. Sulfathiazole 4. Sulfamerazine 5. Sulfamonomethoxine 6. Sulfaquinoxaline

1. Hypoxanthine 2. Xanthine 3. Theobromine 4. Theophylline 5. Caffeine

1. Serine 2. Asparagine 3. Aspartic acid 4. Glutamic acid 5. Alanine 6. Tyrosine 7. Methionine 8. Tryptophan 9. Phenylanaline 10. Leucine

1. Vitamin B1 (thiamine) 2. Vitamin B6 (pyridoxine) 3. Vitamin B3 (nicotinamde) 4. Vitamin B5 (pantothenic acid) 5. Folic Acid 6. Vitamin B12 (cyanocobalamin) 7. Vitamin H (biotin) 8. Vitamin B2 (riboflavin)

Food Safety Water soluble vitamins

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Alternative Selectivity to C18 with Excellent Peak Shape and Sensitivity

Hypersil GOLD PFP (pentafluorophenyl) columns build on the performance of the Hypersil GOLD silica by providing excellentpeak shapes while also offering alternative selectivity in reversed phase chromatography compared to alkyl chain phases. The Hypersil GOLD PFP manufacturing process provides improvements in speed of analysis, peak shape and sensitivity over other fluorinated phases.

Extra Retention for Halogenated Species

Introduction of fluorine groups into the stationary phase causes significant changes in solute-stationary phase interactions.This can lead to extra retention and selectivity for positional isomers of halogenated compounds.

• Analyzing difficult to resolve mixtures of halogenated compounds• Non-halogenated polar aromatic compounds• Analysis of complex taxane samples

Unique Selectivity for Non-halogenated Polar Compounds

Hypersil GOLD PFP Columns are also well suited to the selective analysis of non-halogenated compounds, in particular polar compounds containing hydroxyl, carboxyl, nitro, or other polar groups. High selectivity is often most apparent when thefunctional groups are located on an aromatic or other rigid ring system.

Hypersil GOLD PFP columns are particularly suited to the analysis of compounds containing substituted aromatic rings. This is because the fluorine atoms around the phenyl ring enhance pi-pi interactions increasing retention and selectivity.

Hypersil GOLD PFP Columns Unique selectivity with perfluorinated columns

1. Sulfaguanidine 2. Sulfanilamide 3. Sulfathiazole 4. Sulfamerazine 5. Sulfamonomethoxine 6. Sulfaquinoxaline

1. Hypoxanthine 2. Xanthine 3. Theobromine 4. Theophylline 5. Caffeine

1. Serine 2. Asparagine 3. Aspartic acid 4. Glutamic acid 5. Alanine 6. Tyrosine 7. Methionine 8. Tryptophan 9. Phenylanaline 10. Leucine

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Hypersil GOLD PFP Columns

Column: Hypersil GOLD PFP, 5 µm, 150 × 4.6 mm Mobile Phase: A: Water B: Methanol/acetonitrile (7:93) Gradient: Time (min) % B 0 35 7 35 25 58 Flow Rate: 1.5 mL/min Detection: UV @ 220 nm

Column: Hypersil GOLD PFP, 5 µm, 150 × 4.6 mm Mobile Phase: Water + 0.1% tetrahydrofuran Flow Rate: 1.0 mL/min Temperature: 30 °C Detection: UV @ 220 nm

Column: Hypersil GOLD PFP, 1.9 µm, 50 × 2.1 mm Mobile Phase: A: 25 mM ammonium acetate pH 5.0 B: Acetonitrile Gradient: 10–100% B in 3 mins Flow Rate: 0.5 mL/min Temperature: 40 °C Detection: UV @ 254 nm (2 µL flow cell) Injection Volume: 0.5 µL

Column: Hypersil GOLD PFP, 5 µm, 150 × 4.6 mm Mobile Phase: A: Water + 0.1% formic acid B: Acetonitrile + 1.0% formic acid Gradient: 15–45% B in 20 mins Flow Rate: 1.5 mL/min Temperature: 25 °C Detection: UV @ 270 nm Injection Volume: 5 µm

Fluorinated nucleic bases

Environmental Banned aromatic amines

Phenolic positional isomers

1. Fluorocytosine 2. Fluorouracil

1. 2,4-Diaminotoluene 2. o-Toluidine 3. 4,4-Oxydianiline 4. 2-Methoxy-5-Methylaniline 5. 2,4,5-Trimethylaniline 6. 4,4-Methylene-bis (2-chloroaniline) 7. Impurity from Analyte No. 6

1. 3,4-Dimethoxyphenol 2. 2,6-Dimethoxyphenol 3. 2,6-Difluorophenol 4. 3,5-Dimethoxyphenol 5. 2,4-Difluorophenol 6. 2,3-Difluorophenol 7. 3,4-Difluorophenol 8. 3,5-Dimethoxyphenol 9. 2,6-Dimethoxyphenol 10. 2,6-Dichlorophenol 11. 4-Chloro-3-Methylphenol 12. 3,4-Dichlorophenol 13. 4-Chloro-2-Methylphenol 14. 3,5-Dichlorophenol

1. 10 -Deacetyl baccatin 2. Baccatin III 3. 10-Deacetyl-7-xylosyl taxol B 4. Taxinine M 5. 10-Deacetyl-7-xylosyl taxol 6. 10-Deacetyl taxol 7. 10-Deacetyl-7-xylosyl taxol C 8. 7-Xylosyl taxol 9. Cephalomanine 10. 10-Deacetyl-7epitaxol 11. Paclitaxol 12. Taxol C 13. 7-Epitaxol

Pharmaceutical Taxanes

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Alternative Selectivity with Lower Hydrophobicity than C18

Hypersil GOLD CN columns offer alternative selectivity in reversed phase chromatography with lower hydrophobicity compared to C18 alkyl chain phases. Hypersil GOLD CN columns can also be used in normal phase chromatography, where they offer less retention and different selectivity compared to silica columns.

• Steroids and polyphenols in reversed phase• Surfactants and other polar species in normal phase

Hypersil GOLD CN Columns Cyano columns for reversed and normal phase separations

Column: Hypersil GOLD CN, 5 µm, 150 × 4.6 mm Mobile Phase: A: 10 mM potassium phosphate pH3 B: Acetonitrile Gradient: Time (mins) % B 0 0 1 10 8 70 Flow Rate: 1.25 mL/min Temperature: 25 °C Detection: UV @ 220 nm

Column: Hypersil GOLD CN, 5 µm, 150 × 4.6 mm Mobile Phase: A: 20 mM ammonium formate pH3 B: Acetonitrile Gradient: Time (mins) % B 0 0 15 20 Flow Rate: 1.0 mL/min Temperature: 25 °C Detection: UV @ 254 nm

Pharmaceutical Penicillins

TB Drugs

1. N-acetyl Penicillamine 2. Ampicillin 3,4,5. Impurities from Penicillin G 6, Penicillin G

1. Isoniazid 2. Pyrazinamide

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Column: Hypersil GOLD CN, 5 µm, 150 × 4.6 mm Mobile Phase: A: Water B: Acetonitrile Gradient: Time (mins) % B 0 10 15 50 Flow Rate: 1.5 mL/min Temperature: 25 °C Detection: UV @ 254 nm

Column: Hypersil GOLD CN, 5 µm, 150 × 4.6 mm Mobile Phase: A: 25 mM potassium phosphate pH2 B: Methanol Isocratic: 95% A: 5% B Flow Rate: 1.5 mL/min Temperature: 25 °C Detection: UV @ 230 nm

Organic acids

1. 4-Fluorobenzoic 2. o-Toluic Acid 3. p-Toluic Acid 4. 2,4,6-Trimethylbenzoic Acid 5. 2,5-Dimethylbenzoic Acid

1. Hydrocortisone 2. Cortisone 3. Corticosterone 4. 11-α Hydroxprogesterone 5. 17-α Hydroxprogesterone 6. Progesterone 7. Deoxycorticosterone

Toxicology Steroids

Hypersil GOLD CN Columns

Alternative Selectivity for Aromatic and Moderately Polar Analytes

Our Hypersil GOLD Phenyl reversed phase HPLC columns exhibit alternative selectivity to alkyl chain columns, particularlyfor aromatic and moderately polar analytes.

Enhanced Pi-pi Interactions with Aromatics

Many phenyl phases use a propyl (C3) linker between the silica and the phenyl ring. The Hypersil GOLD Phenyl bonded phasecontains a butyl (C4) linker which allows for superior alignment of the phenyl ring with aromatic molecules, enhancing pi-piinteractions and therefore their retention.

• Analyte mixtures with varying polarity and aromaticity• Where alternative selectivity to C18 is required

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Hypersil GOLD Phenyl Columns Excellent retention and unique selectivity for aromatic analytes

Moderate Hydrophobicity

The C4 linker also provides the stationary phase with moderate hydrophobicity, making it ideal for the separation of analytemixtures with varying polarity and aromaticity.

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Hypersil GOLD Phenyl Columns

Column: Hypersil GOLD Phenyl, 5 µm, 150 × 4.6 mm Mobile Phase: A: 20 mM potassiumn phosphate pH 2.5 B: Acetonitrile Gradient: 20–50% B in 15 mins Flow Rate: 1 mL/min Temperature: 30 °C Injection Volume: 5 µL Detection: UV @ 225 nm

Column: Hypersil GOLD Phenyl, 5 µm, 150 × 4.6 mm Mobile Phase: 20 mM potassium phosphate pH 7.0/ acetonitrile (80/20) Flow Rate: 1 mL/min Temperature: 25 °C Injection Volume: 5 µL Detection: UV @ 254 nm

Column: Hypersil GOLD Phenyl, 5 µm, 150 × 4.6 mm Mobile Phase: A: Water B: Methanol Gradient: 40–70% B in 10 mins Flow Rate: 1 mL/min Temperature: 25 °C Injection Volume: 5 µL Detection: UV @ 260 nm

Column: Hypersil GOLD Phenyl, 1.9 µm, 50 × 2.1 mm Mobile Phase: A: 0.1% formic acid B: 0.1% formic acid in acetonitrile Gradient: 10–60% B in 3.4 min 60–90% B in 0.24 min Flow Rate: 0.5 mL/min Temperature: 60 °C Injection Volume: 0.7 µL Detection: UV @ 225 nm and 254 nm

Antacids

Veterinary drug coccidiostats

Antidepressants

1. Famotidine 2. Cimetidine 3. Ranitidine

1. 4-amino-3,5-dinitrobenzamide 2. Zoalene (3,5-nitro-o-toluamide 3. Nitromid (3,5-dinitrobenzamide) 4. Ethopabate

1. Uracil 2. Acetaminophen 3. p-Hydroxybenzoic acid 4. o-Hydroxybenzoic acid 5. Oxazepam 6. Diazepam 7. Di-isopropyl phthalate 8. Di-n-propyl phthalate

1. Carbadox 2. Thiamphenicol 3. Furazolidone 4. Oxolinic Acid 5. Sulfadimethoxine 6. Sulfaquinoxaline 7. Nalidixic Acid 8. Piromidic Acid

Pharmaceutical Antibacterials

Excellent Chromatographic Properties in Four Modes: Weak Anion Exchange, Reversed Phase, HILIC and Normal Phase

Hypersil GOLD Amino columns can be used with common buffers and an organic modifier as a weak ion exchange material for the analysis of anions and organic acids. When used under normal phase conditions, Hypersil GOLD Amino columns offer an alternative selectivity to silica. Hypersil GOLD Amino columns excel for carbohydrate analysis when used in HILIC mode.

• Retains anions and organic acids in weak anion exchange• Excellent for carbohydrate analysis in HILIC

19

Hypersil GOLD Amino

175

L8

2 to 8

2

Hypersil GOLD Amino Columns Highly versatile aminopropyl stationary phase

Column: Hypersil GOLD Amino, 5 μm, 150 × 4.6 mm Mobile Phase: Acetonitrile/water (80:20) Flow Rate: 1.2 mL/min Temperature: 35 °C Detection: RI Injection Volume: 20 μL

Column: Hypersil GOLD Amino, 5 µm, 150 × 4.6 mm Mobile Phase: Acetonitrile/water (80:20) Flow Rate: 1.2 mL/min Temperature: 35 °C Detection: RI Injection Volume: 20 µL

Food Safety Sugars

Sorbitol

1. Fructose 2. Glucose 3. Sucrose 4. Maltose 5. Lactose

1. Sorbitol

14

12

0

2

4

6

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uRIU

Minutes0 2 4 6 8 10 12 14 16 18

1

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uRIU

Outstanding Peak Shape and Sensitivity

Based on the same highly pure silica backbone, Hypersil GOLD Amino columns offer improved peak shape over type A silicacolumns. For high speed, high efficiency separations, Hypersil GOLD Amino columns are available with 1.9 μm particle size.

20

Weak Anion Exchange Phase

Hypersil GOLD AX columns utilise a novel polymeric amine ligand bonded to highly pure base deactivated silica. The silicasubstrate brings higher efficiency than polymer based ion exchange columns.

• Smaller proteins and peptides• Anionic species• Polar molecules

Hypersil GOLD AX

175

2 to 8

6

Hypersil GOLD AX Columns Separation of anionic species and polar molecules

Suitable for HILIC

Hypersil GOLD AX columns are particularly suited to the analysis of polar compounds in HILIC applications. For high speed, high efficiency separations, Hypersil GOLD AX columns are available with 1.9 µm particle size.

Column: Hypersil GOLD AX, 5 µm, 150 × 4.6 mm Mobile Phase: Aqueous phosphate buffer (50 mM, pH 3) Flow Rate: 1.0 mL/min Temperature: 40 °C Detection: UV @ 254 nm Injection Volume: 10 µL

Column: Hypersil GOLD AX, 5 µm, 100 × 4.6 mm Mobile Phase: 100 mM ammonium acetate pH 6.8/ acetonitrile (30:70) Flow Rate: 0.5 mL/min Temperature: 30 °C Detection: UV @ 240 nm Injection Volume: 50 µL

Biopharma Monophosphates

Food Safety Vitamin C

Minutes0 2 4 6 8 10

1

23

4

150

125

100

75

50

25

0

mAU

Minutes250 5 10 15 20

mAU

100

0

20

40

60

801

1. Uracil 2. Cytidine-5’-monophosphate 3. Adenosine-5’-monophosphate 4. Guanosine-5’-monophosphate

1. Vitamin C

21

High Stability to Aqueous Mobile Phase

The Hypersil GOLD SAX stationary phase utilises a highly stable quaternary amine strong anion exchange ligand bonded to highly pure silica. Hypersil GOLD SAX columns are suited to the analysis of smaller organic molecules such as nucleotidesand organic acids using aqueous and low pH mobile phases.

• Smaller organic molecules• Ionic species

Hypersil GOLD SAX

175

L14

2 to 8

2.5

1

2

3

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0 1 2 3 4 5 6 7 8Minutes

175

150

125

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75

50

25

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mA

U

Hypersil GOLD SAX Columns Quaternary amine strong anion exchange column

Outstanding Peak Shape and Sensitivity

Based on the same highly pure silica backbone, Hypersil GOLD SAX columns offer improved peak shape over type A silicacolumns. For high speed, high efficiency separations, Hypersil GOLD SAX columns are available with 1.9 μm particle size.

Column: Hypersil GOLD SAX, 5 μm, 150 × 4.6 mm Mobile Phase: 50 mM phosphate buffer pH 3.0 Flow Rate: 1.0 mL/min Detection: UV @ 254 nm Column Temperature: 40 °C Injection Volume: 10 µL

Biopharma Monophosphates 1. Uracil

2. Cytidine-5’-monophosphate 3. Adenosine-5’-monophosphate 4. Guanosine-5’-monophosphate

22

Outstanding Peak Shape and Sensitivity

Unbonded, highly pure base deactivated silica media that is the backbone of the Hypersil GOLD range of columns. Hypersil GOLD Silica columns are a powerful and efficient tool for the chromatography of non-polar and moderately polar organic compounds by normal phase chromatography. For high speed, high efficiency separations, Hypersil GOLD Silica columns are available with 1.9 μm particle size.

Hypersil GOLD Silica Columns Excellent peak shape in normal phase chromatography

Minutes

mAU

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

90

80

70

60

50

40

30

20

10

0

1

2

3

4

5

6 7

Hypersil GOLD Silica

175

L3

2 to 8

• Steroids in normal phase• Polar analytes in HILIC

Column: Hypersil GOLD Silica, 5 μm, 150 × 4.6 mm Mobile Phase: Hexane/ethanol (19:1) Flow Rate: 1.5 mL/min Temperature: 30 °C Detection: UV @ 254 nm Injection Volume: 5 μL

Forensics Steroids 1. Progesterone

2. 21-Hydroxyprogesterone -21-acetate 3. 17-a-Hydroxyprogesterone 4. Cortisone 5. 11-a-Hydroxyprogesterone 6. Corticosterone 7. Hydrocortisone

• Polar and hydrophilic compounds • Carbohydrates• Enhanced sensitivity in MS

23

2 to 8

6

175

Hypersil GOLD HILIC

Hypersil GOLD HILIC Columns Enhanced retention of polar and hydrophilic analytes

Enhanced Retention of Polar and Hydrophilic Analytes

Hydrophilic interaction liquid chromatography (HILIC) is an increasingly popular technique offering complementary selectivity to reversed-phase. With the ability to retain highly polar and hydrophilic compounds, Hypersil GOLD HILIC columns have been developed to aid the analysis of compounds that are traditionally difficult to retain using conventional C18 columns.In HILIC, by incorporating water in the highly organic mobile phase, an adsorbed water-rich layer is formed on the polarstationary phase surface into which analyte molecules partition. Retention is governed by dipole-dipole interactions andhydrogen bonding mechanisms.

Column: Hypersil GOLD HILIC, 5 µm, 150 × 4.6 mm Mobile Phase: Water/acetonitrile (10:90) + 0.1% formic acid Flow Rate: 1.0 mL/min Temperature: ambient Detection: UV @ 205, 230 & 260 nm Injection Volume: 10 µL

Column: Hypersil GOLD HILIC, 5 µm, 150 × 4.6 mm Mobile Phase: Water/acetonitrile (10:90) + 0.1% formic acid Flow Rate: 0.6 mL/min Temperature: 30 °C Detection: +ESI Injection Volume: 1 µL (made up in mobile phase)

Food Safety Water soluble vitamins

ChemicalUrea

1. Thiamine 2. Nicotinic acid 3. Nicotinamide 4. Pyridoxine 5. Riboflavin 6. PABA.

1. Urea

Minutes1 2 3 4 5 60 7 8 9 10

1

2

3

45

7

6

5

4

3

2

1

0

mAU

1

0 1 2 3 4 5 6 7 8 9

Minutes

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80

90

100

Rel

ativ

e Ab

unda

nce

Improved Sensitivity with MS Detection

The highly organic mobile phases containing low salt levels used for HILIC, make Hypersil GOLD HILIC columns ideal for use with electrospray mass spectroscopy.

1. Progesterone 2. 21-Hydroxyprogesterone -21-acetate 3. 17-a-Hydroxyprogesterone 4. Cortisone 5. 11-a-Hydroxyprogesterone 6. Corticosterone 7. Hydrocortisone

24

The Power of 1.9 μm Particles

1.9 μm particles give higher efficiency than 3 μm or 5 μm particles and this efficiency is delivered over a greater range of optimum linear velocity. This makes it possible to operate at higher flow rates without losing performance. Because shorter columns packed with 1.9 μm particles give equivalent efficiency to longer columns packed with 5 μm particles faster analysis and solvent savings for the chromatographer become a reality.

Hypersil GOLD 1.9 μm Small particles to improve speed and efficiency

Pressure Rating of Hypersil GOLD 1.9 μm Columns

Column Hardware Pressure Rating

Analytical columns 1250 bar/18000 psi

Capillary/nano columns 400 bar/6000 psi

Javelin HTS columns 400 bar/6000 psi

Three Tips for Method Transfer

1. To maintain an equivalent separation when transferring a method it is important to keep the reduced linear velocity constant between the original and new method.

2. Sub-2 μm based methods are most often transferred to smaller volume columns, so the same injection volume will take up a larger proportion of the new column, possibly leading to band broadening. It is therefore important to scale down the injection volume to match the change in column volume.

3. Geometrical transfer of the gradient requires calculation of the number of column volumes of mobile phase in each segment (time interval) of the gradient in the original method to ensure that the new calculated gradient takes place over the same number of column volumes, for the new column.

HPLC Method Development Calculatorwww.thermoscientific.com/crc

Hypersil GOLD 1.9 μm

1

23

4

5

6

7

0 2 4 6 8 10 12 14Minutes

4 x FasterHigher Resolution

P at to = 585 bar

12.3 minRs (5,6) = 2.8

1

2 3

4

5

67

0 5 10 15 20 25 30 35Minutes

40 45 50

46.5 minRs (5,6) = 1.6

HPLC: 150 x 4.6 mm, 5 μm

1

2 3

4

5

6

7

0 1 2 3 4 5Minutes

6 7 8

P at to = 330 bar

6.5 minRs (5,6) = 1.6

UHPLC: 50 x 2.1 mm, 1.9 μm

7 x FasterSame Resolution

UHPLC: 100 x 2.1 mm, 1.9 μm

Speed

10 mm HTS

20 mm

30 mm

50 mm

100 mm 150 mm 200 mm

Efficiency

Which 1.9 μm Column?

We offer an extensive range of columns packed with 1.9 µm particles to suit the full variety of application needs.The choice of column will depend upon the requirement of the analysis. • Speed: choose from 10 mm Javelin HTS, 20, 30 or 50 mm long analytical columns• Efficiency: choose a longer column (for example 150 or 200 mm)• Low backpressure: Hypersil GOLD 1.9 µm media is packed into a high pressure column 50 mm long and 4.6 mm internal diameter. Traditionally, a 1.9 µm column is used on UHPLC instruments. However, by producing less backpressure, this new wider column is suitable for users of conventional systems where pressure limits are often in the 6000 psi/400 bar region, ensuring fast chromatography without the need for extensive instrument optimization.

Transferring a method using these tips can give results as shown below for the separation of Ibuprofen and impurities.

25

26

Hypersil GOLD 1.9 μm Javelin HTS Columns for Speed

Hypersil GOLD 1.9 μm Javelin HTS columns take fast LC to the extreme. These short 10 mm columns enable analysis times as

fast as 8 seconds to be achieved. The use of ultra-low dead volume, direct connect Javelin hardware also minimizes dispersion.

1

Column: Hypersil GOLD 1.9 µm, 10 × 2.1 mm Mobile Phase: Warer/acetonitrile 40/60 + 0.1% tetrahydrofuran isocratic Flow Rate: 0.4 mL/min Temperature: 5 °C Detection: 254 nm Injection Volume: 0.5 µL

Toxicology Nandrolone 1. Nandrolone

(19-Nortestosterone)

200 mm Column for Efficiency

The 1.9 μm particles used in Hypersil GOLD columns give less backpressure than 1.8 or 1.7 μm, permitting the use of longer

columns for greater efficiency.

System Considerations

With 1.9 μm particles, analyses can be performed with a high linear velocity through the column without loss in performance,provided the LC system is optimized to operate under these conditions. In order to produce fast, efficient chromatography,all system components for the assay should also be considered. Modern ultra high pressure liquid chromatography (UHPLC)instruments, including the Thermo Scientific™ Dionex™ UltiMate™ 3000 HPLC system, will take account of these factors.

There are three major system considerations to remember when using short columns packed with 1.9 µm particles.

1. The system volume (connecting tubing ID and length, injection volume, UV detector flow cell volume) must be minimized2. The detector time constant and sampling rate need to be carefully selected3. When running fast gradients pump delay volume needs to be minimal.

0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30

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200

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350

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8 9

Column: Hypersil GOLD 1.9 µm, 200 × 2.1 mm Mobile Phase: A: Water + 0.1% formic acid B: Acetonitrile + 0.1% formic acid Gradient: 5 to 95% B in 24 min Flow Rate: 0.6 ml/min Temperature: 60 °C Injection Volume: 1 µL Detection: UV at 270 nm Pressure: 606 bar

Environmental Phenolic pollutants 1. Phenol

2. 4-nitrophenol 3. 2-nitrophenol 4. 4-chlorophenol 5. 2-chlorophenol 6. 2,6-dimethylphenol 7. 2,4-dimethylphenol 8. 2,4-dichlorophenol 9. Pentachlorophenol

Hypersil GOLD 1.9 μm

Hypersil GOLD columns are available in particle sizes and column designs to meet all separation needs, including improved resolution, enhanced sensitivity, and faster analyses. With particle sizes from 1.9 μm to 12 μm, Hypersil GOLD columns offer chromatographic solutions with consistent separations and performance. Specialized hardware includes:

• Preparative columns• Thermo Scientific™ Javelin™ HTS direct-connection columns• Guard columns for column protection

Preparative Columns

Analytical methods may require scale up to preparative sizes to isolate and purify compounds from mixtures. In choosing the best column and packing material for your preparative application, consider:

• Selectivity• Loadability of the media• Column dimensions

We have established a strong reputation for the manufacture and supply of high quality preparative columns, designed to give the same levels of performance and reproducibility as our popular analytical columns. Scale up is easiest when starting from an analytical column packed with smaller particle size media offering the same selectivity as the larger particle size preparative media. Hypersil GOLD phases are offered in various sizes to complement lab scale operations and facilitate the scale up to preparative chromatography. Contact us for ordering details on Hypersil GOLD preparative columns.

Columns for High Throughput ScreeningJavelin HTS columns are specifically designed for high throughput applications. Using finger tight fittings and low dead volume hardware to minimise band broadening, these columns are ideal for ballistic gradients, providing enough retention and sensitivity for very fast assays. Javelin HTS columns are available in multipacks to provide a cost effective solution.

Hardware Solutions Hardware solutions for high throughput screening, capillary and preparative chromatography

27

1. Nandrolone (19-Nortestosterone)

1. Phenol 2. 4-nitrophenol 3. 2-nitrophenol 4. 4-chlorophenol 5. 2-chlorophenol 6. 2,6-dimethylphenol 7. 2,4-dimethylphenol 8. 2,4-dichlorophenol 9. Pentachlorophenol

Description Particle Size Length (mm) ID (mm) Part Number

Hypersil GOLD Javelin HTS Column (3/pk) 1.9 10 2.1 25002-012135

5 20 4.0 25005-024035

Javelin HTS Column

28

Guard Columns

Drop-in guard cartridges and holders offer convenience, economy, and effective protection for extending analytical column lifetimes. The 10 mm design offers maximum protection with minimal increase in retention. Hypersil GOLD drop-in guard cartridges are provided in packs of 4 each. Ordering details on the following pages.

UHPLC FilterReplaceable 0.2 μm Thermo Scientific UHPLC filter cartridges can be used to protect Hypersil GOLD 1.9 μm columns against particulate contamination, extending column lifetime. It’s low dead volume design maintains chromatographic performance without degrading peak shape and causes minimal efficiency loss through dispersion. The UHPLC filter adds minimal increase in backpressure and so can be fitted to any length column.

Column Protection Extend column lifetime and improve performance

Description Length (mm) ID (mm) Part Number

UNIGUARD Guard Cartridge Holder 10 1.0 851-00

2.1 852-00

3.0 852-00

4.0/4.6 850-00

Part Number

UNIGUARD Guard Cartridge Holder

Description ID (mm) Part Number

UHPLC Filter Holder 27006

UHPLC Filter Cartridge, 0.2 µm (5/pk) 2.1 22180

1.0 22185

29

Ordering InformationHypersil GOLD HPLC ColumnsParticle Size (µm) Description Length (mm) ID (mm) Hypersil GOLD Hypersil GOLD C8 Hypersil GOLD PFP

1.9 UHPLC Column 20 2.1 25002-022130 25202-022130 25402-022130 30 1.0 25002-031030 – – 2.1 25002-032130 25202-032130 25402-032130 50 1.0 25002-051030 25202-051030 25402-051030 2.1 25002-052130 25202-052130 25402-052130 3.0 25002-053030 25202-053030 25402-053030 4.6 25002-054630 25202-054630 25402-054630 100 1.0 25002-101030 25202-101030 25402-101030 2.1 25002-102130 25202-102130 25402-102130 3.0 25002-103030 25202-103030 25402-103030 150 2.1 25002-152130 25202-152130 25402-152130 200 2.1 25002-202130 25202-202130 25402-2021303 Drop-in Guard (4/pk) 10 1.0 25003-011001 25203-011001 25403-011001 2.1 25003-012101 25203-012101 25403-012101 3.0 25003-013001 25203-013001 25403-013001 4.0/4.6 25003-014001 25203-014001 25403-014001 HPLC Column 30 2.1 25003-032130 25203-032130 25403-032130 3.0 25003-033030 25203-033030 25403-033030 4.6 25003-034630 25203-034630 25403-034630 50 2.1 25003-052130 25203-052130 25403-052130 3.0 25003-053030 25203-053030 25403-053030 4.0 25003-054030 25203-054030 25403-054030 4.6 25003-054630 25203-054630 25403-054630 100 1.0 25003-101030 25203-101030 25403-101030 2.1 25003-102130 25203-102130 25403-102130 3.0 25003-103030 25203-103030 25403-103030 4.0 25003-104030 25203-104030 25403-104030 4.6 25003-104630 25203-104630 25403-104630 150 1.0 25003-151030 25203-151030 25403-151030 2.1 25003-152130 25203-152130 25403-152130 3.0 25003-153030 25203-153030 25403-153030 4.0 25003-154030 25203-154030 25403-154030 4.6 25003-154630 25203-154630 25403-1546305 Drop-in Guard (4/pk) 10 2.1 25005-012101 25205-012101 25405-012101 3.0 25005-013001 25205-013001 25405-013001 4.0/4.6 25005-014001 25205-014001 25405-014001 HPLC Column 30 2.1 25005-032130 – – 3.0 25005-033030 – – 4.6 25005-034630 – – 50 2.1 25005-052130 25205-052130 25405-052130 3.0 25005-053030 25205-053030 25405-053030 4.6 25005-054630 25205-054630 25405-054630 100 2.1 25005-102130 25205-102130 25405-102130 3.0 25005-103030 25205-103030 25405-103030 4.6 25005-104630 25205-104630 25405-104630 150 2.1 25005-152130 25205-152130 25405-152130 3.0 25005-153030 25205-153030 25405-153030 4.0 25005-154030 25205-154030 25405-154030 4.6 25005-154630 25205-154630 25405-154630 250 2.1 25005-252130 25205-252130 25405-252130 3.0 25005-253030 25205-253030 25405-253030 4.0 25005-254030 25205-254030 25405-254030 4.6 25005-254630 25205-254630 25405-254630

30

Hypersil GOLD HPLC ColumnsParticle Size (µm) Description Length (mm) ID (mm) Hypersil GOLD aQ Hypersil GOLD C4 Hypersil GOLD CN

1.9 UHPLC Column 20 2.1 25302-022130 – – 30 1.0 – – – 2.1 25302-032130 – – 50 1.0 25302-051030 – – 2.1 25302-052130 25502-052130 25802-052130 3.0 25302-053030 – – 4.6 25302-054630 – – 100 1.0 25302-101030 – – 2.1 25302-102130 25502-102130 25802-102130 3.0 25302-103030 – – 150 2.1 25302-152130 25502-152130 25802-152130 200 2.1 25302-202130 25502-202130 25802-2021303 Drop-in Guard (4/pk) 10 1.0 25303-011001 25503-011001 25803-011001 2.1 25303-012101 25503-012101 25803-012101 3.0 25303-013001 25503-013001 25803-013001 4.0/4.6 25303-014001 25503-014001 25803-014001 HPLC Column 30 2.1 25303-032130 25503-032130 25803-032130 3.0 25303-033030 – – 4.6 25303-034630 25503-034630 25803-034630 50 2.1 25303-052130 25503-052130 25803-052130 3.0 25303-053030 – – 4.0 25303-054030 – – 4.6 25303-054630 – – 100 1.0 25303-101030 – – 2.1 25303-102130 25503-102130 25803-102130 3.0 25303-103030 25503-103030 25803-103030 4.0 25303-104030 – – 4.6 25303-104630 25503-104630 25803-104630 150 1.0 25303-151030 25503-151030 25803-151030 2.1 25303-152130 25503-152130 25803-152130 3.0 25303-153030 25503-153030 25803-153030 4.0 25303-154030 – – 4.6 25303-154630 25503-154630 25803-1546305 Drop-in Guard (4/pk) 10 2.1 25305-012101 25505-012101 25805-012101 3.0 25305-013001 25505-013001 25805-013001 4.0/4.6 25305-014001 25505-014001 25805-014001 HPLC Column 30 2.1 – – – 3.0 – – – 4.6 – – – 50 2.1 25305-052130 25505-052130 25805-052130 3.0 25305-053030 – – 4.6 25305-054630 25505-054630 25805-054630 100 2.1 25305-102130 25505-102130 25805-102130 3.0 25305-103030 25505-103030 25805-103030 4.6 25305-104630 25505-104630 25805-104630 150 2.1 25305-152130 25505-152130 25805-152130 3.0 25305-153030 – – 4.0 25305-154030 – – 4.6 25305-154630 25505-154630 25805-154630 250 2.1 25305-252130 25505-252130 25805-252130 3.0 25305-253030 25505-253030 25805-253030 4.0 25305-254030 25505-254030 25805-254030 4.6 25305-254630 25505-254630 25805-254630

31

Hypersil GOLD HPLC ColumnsParticle Size (µm) Description Length (mm) ID (mm) Hypersil GOLD Phenyl Hypersil GOLD Amino Hypersil GOLD AX

1.9 UHPLC Column 20 2.1 – – – 30 1.0 – – – 2.1 – – – 50 1.0 – – – 2.1 25902-052130 25702-052130 26102-052130 3.0 – – – 4.6 – – – 100 1.0 – – – 2.1 25902-102130 25702-102130 26102-102130 3.0 – – – 150 2.1 25902-152130 25702-152130 26102-152130 200 2.1 25902-202130 25702-202130 26102-2021303 Drop-in Guard (4/pk) 10 1.0 25903-011001 25703-011001 26103-011001 2.1 25903-012101 25703-012101 26103-012101 3.0 25903-013001 25703-013001 26103-013001 4.0/4.6 25903-014001 25703-014001 26103-014001 HPLC Column 30 2.1 25903-032130 25703-032130 26103-032130 3.0 – – – 4.6 25903-034630 25703-034630 26103-034630 50 2.1 25903-052130 25703-052130 26103-052130 3.0 – – – 4.0 – – – 4.6 – – – 100 1.0 – – – 2.1 25903-102130 25703-102130 26103-102130 3.0 25903-103030 25703-103030 26103-103030 4.0 – – – 4.6 25903-104630 25703-104630 26103-104630 150 1.0 25903-151030 25703-151030 26103-151030 2.1 25903-152130 25703-152130 26103-152130 3.0 25903-153030 25703-153030 26103-153030 4.0 – – – 4.6 25903-154630 25703-154630 26103-1546305 Drop-in Guard (4/pk) 10 2.1 25905-012101 25705-012101 26105-012101 3.0 25905-013001 25705-013001 26105-013001 4.0/4.6 25905-014001 25705-014001 26105-014001 HPLC Column 30 2.1 – – – 3.0 – – – 4.6 – – – 50 2.1 25905-052130 25705-052130 26105-052130 3.0 – – – 4.6 25905-054630 25705-054630 26105-054630 100 2.1 25905-102130 25705-102130 26105-102130 3.0 25905-103030 25705-103030 26105-103030 4.6 25905-104630 25705-104630 26105-104630 150 2.1 25905-152130 25705-152130 26105-152130 3.0 – – – 4.0 – – – 4.6 25905-154630 25705-154630 26105-154630 250 2.1 25905-252130 25705-252130 26105-252130 3.0 25905-253030 25705-253030 26105-253030 4.0 25905-254030 25705-254030 26105-254030 4.6 25905-254630 25705-254630 26105-254630

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Hypersil GOLD HPLC ColumnsParticle Size (µm) Description Length (mm) ID (mm) Hypersil GOLD SAX Hypersil GOLD Silica Hypersil GOLD HILIC

1.9 UHPLC Column 20 2.1 – – – 30 1.0 – – – 2.1 – – – 50 1.0 – – – 2.1 26302-052130 25102-052130 26502-052130 3.0 – – – 4.6 – – – 100 1.0 – – – 2.1 26302-102130 25102-102130 26502-102130 3.0 – – – 150 2.1 26302-152130 25102-152130 26502-152130 200 2.1 26302-202130 25102-202130 26502-2021303 Drop-in Guard (4/pk) 10 1.0 26303-011001 25103-011001 26503-011001 2.1 26303-012101 25103-012101 26503-012101 3.0 26303-013001 25103-013001 26503-013001 4.0/4.6 26303-014001 25103-014001 26503-014001 HPLC Column 30 2.1 26303-032130 25103-032130 26503-032130 3.0 – – – 4.6 26303-034630 25103-034630 26503-034630 50 2.1 26303-052130 25103-052130 26503-052130 3.0 – – – 4.0 – – – 4.6 – – – 100 1.0 – – – 2.1 26303-102130 25103-102130 26503-102130 3.0 26303-103030 25103-103030 26503-103030 4.0 – – – 4.6 26303-104630 25103-104630 26503-104630 150 1.0 26303-151030 25103-151030 26503-151030 2.1 26303-152130 25103-152130 26503-152130 3.0 26303-153030 25103-153030 26503-153030 4.0 – – – 4.6 26303-154630 25103-154630 26503-1546305 Drop-in Guard (4/pk) 10 2.1 26305-012101 25105-012101 26505-012101 3.0 26305-013001 25105-013001 26505-013001 4.0/4.6 26305-014001 25105-014001 26505-014001 HPLC Column 30 2.1 – – – 3.0 – – – 4.6 – – – 50 2.1 26305-052130 25105-052130 26505-052130 3.0 – – – 4.6 26305-054630 25105-054630 26505-054630 100 2.1 26305-102130 25105-102130 26505-102130 3.0 26305-103030 25105-103030 26505-103030 4.6 26305-104630 25105-104630 26505-104630 150 2.1 26305-152130 25105-152130 26505-152130 3.0 – – – 4.0 – – – 4.6 26305-154630 25105-154630 26505-154630 250 2.1 26305-252130 25105-252130 26505-252130 3.0 26305-253030 25105-253030 26505-253030 4.0 26305-254030 25105-254030 26505-254030 4.6 26305-254630 25105-254630 26505-254630

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TG20762_E 03/13S