1. Human umbilical cord mesenchymalstem cells attenuate cisplatin-inducedacute and chronic renalinjury

2. INTRODUCTION 3. MATERIALS AND METHODShucMSCs isolation and identificationCM-Dil cell-labellingCell co-cultureCisplatin-induced renal injury rat model (6.0 mg/kg)hucMSCs transplantation (intraperitoneally injection)Renal function and Malondialdehyde (MDA) determinationHistopathology and Massons trichrome stainingTUNEL staining and immunohistochemistryQuantitative RT-PCRWestern blot 4. hucMSCs accelerate the recovery of renal function in cisplatin-inducedAKI rats 5. hucMSCs promote proliferation of and inhibitapoptosis and alleviate inflammation in cisplatin-inducedAKI rats in the renal tubular cells of cisplatin-inducedAKI rats 6. hucMSCs protect renal cell mitochondria fromdysfunction and inhibit the activation of mitochondrialapoptosis signaling 7. Effects of hucMSCs on rat renal function indexes and histology 8. Inhibition of renal interstitial fibrosis by hucMSC injection 9. hucMSCs inhibit epithelial-mesenchymal transition inrenal cells in vivo and in vitro 10. ConclusionIn conclusion,results demonstrate that hucMSCs attenuate cisplatin-induced acuteand chronic renal injury through preventing mitochondria dysfunction, inhibitingthe activation of mitochondrial signaling pathway and suppressing thedevelopment of EMT.These findings suggest that hucMSC transplantation is a useful approach for thetherapy of cisplatin-induced AKI and the subsequent renal interstitial fibrosis.