cm

Y

Highly Potent Rabbit Anti-(mouse Lymphocyte)/era and

Rabbit Rnti-(mouseThymocyte)/era

To meet the needs of investigators who require highly active immuno- suppression with low toxicity, this sera is now available. Each lot of anti- (lymphocyte) serum (ALS) and anti-(thymocyte) serum (ATS) is assayed by means of skin allograft survival and the assay results are enclosed with each shipment. A typical assay of Rabbit ATS showed a mean survival time (MST) of 24.2 days, while a typical assay of Rabbit ALS had not reached its MST even 33 days post-allotransplantation. Horse Anti-Mouse Lymphocyte Serum also provides significant immuno-suppression and is assayed in the same manner as Rabbit ALS. A typical assay showed a graft survival of 17.2 days (MST)

MICROBIOLOGICAL ASSOCIATES S U B S I D I A R Y O F ~ ' ~ C ~ I ~ S C O R P O R A T I O N

4733 Bethesda Avenue, Bethesda, Maryland 20014 (301) 654-3400 503 San Pable Avenue, Albany, California 94706 (415) 526-6228

Serving the Biomedical Field Since 1947

X

THE J O U R N A L OF

IMMUNOLOGY

VOLUME 107 NUMBERS 4 TO 6

BALTIMORE, MD. 21202 1971

CoPYmQnT ~), 1971, BY THE WILLIAMS & WIL]1NS COMPANT

T~s J o ~ oP I~KVNOLOeY Vol. 107, No. 6, December 1971 Copyright ~ 1971 by The Williams & WUkin~ Co. P r i ~ in U.8.A.

CONTENTS

N ~ B E R 1, JULY 1971

IMMUNOCHEMISTRY

The Evolution of the Immune Response. XII . The Trnmunoglobulins of the Turtle. Molecular Re- quirements for Biologic Activity of 5.7S lmmunoglobulin. S .L . Chartrand, G. W. Litman, N. Lapointe, R. A. Good and D. Frommel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Comparative Ligand-Binding by Purified Nurse Shark Natural Antibodies and Anti-2,4-Dinitro- phenyl Antibodies from Other Species. Edward W. Voss, Jr., Stuart Rudikoff and M. M. Sigel . . . . . . . . . . . . . . . . . . . . . . ~ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Deficiency of C3 Inactivator in Man. Neil Abramson, Chester A. Alper, Peter J. Lachmann, Fred S. Rosen and James H. Jandl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Kinetics of Bovine Complement. I. Formation of a Lytic Intermediate. Jack S. C. Fong, Louis H. Muschel and Robert A. Good . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Kinetics of Bovine Complement. II. Properties of the Lytic Intermediate. Jack S. C. Fong, Louis H. Muschel and Robert A. Good . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Presence in Rabbit Colostrum of IgA Autoantibodies to a Gastric Antigen. Ronald D. Wise, Edward A. Liehter and Sheldon Dray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

The Immune Response in the Hamster. . Biologic Activities of 7S-tl and 7ST2 Globulins. J. Coe, L. Peel and R. F. Smith . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

Antibody Formation against ,gaZmonella Flagella by an Immune Ribonucleic Acid Fraction. Nobuo Yamaguchi, Satonori Kurashige and Susumu Mitsuhashi . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

Further Characterization of Purified Fractions of M Protein from a Strain of Group A, Type 12 ~treptococcus. Kenneth L. Vosti, Rudolph H. Johnson and Michael F. Dillon . . . . . . . . . . . . . . . 104

IgA and Other Immunoglobulins from the European Hedgehog, J. P. Vaerman and J. F. Heremans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 201

Specificity of Anti-Poly G.Poly C Antibodies. E. Nahon-Merlin, A. M. Michelson, C. Verger and F. Lacour . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 222

IMMUNOGENETICS

Conversion of Non-Immune Rabbit Spleen Cells by Ribonucleie Acid of Lymphoid Ceils from an Immunized Rabbit to Produce IgM and IgG Antibody of Foreign Heavy-Chain Allotype. Clara Bell and Sheldon Dray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

Genetic Polymorphism in Rhesus C3 and Ge Globulin. Chester A. Alper, Noel I. Robin and Samuel Refetoff . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

Serum Antigens of Cattle. II. lmmunogenetics of Two Immunoglobulin Allotypes. Dennis Blakeslee, John E. Butler and W. H. Stone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 227

CELLULAR IMMUNOLOGY

Proliferation of Maximally Activated Antigen-Primed Cells: A Computer Simulation. Stanley Cohen and Martin Milgrom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115

Antigenic Relationship between Human IgE lmmunoglobulin and Rat Homocytotropic Antibody. Athanasia Liakopoulou and Lewis Perelmutter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131

Cutaneous Basophil Hypersensitivity. III . Participation of the Basophil in Hypersensitivity to Antigen-Antibody Complexes. Delayed Hypersensitivity and Contact Allergy. Passive Transfer. Harold F. Dvorak, Blanche A. Simpson, Robert C. Bast, Jr. and Sidney Leskowitz. 138

Comparison of Lymphocyte Transformation, Inhibition of Macrophage Migration and Skin Tests Using Dialyzable and Nondialyzable Tuberculin Fractions from Mycobacterium bods (BCG). Sotiros D. Chaparas, Daniel E. Thor and Sally R. Hedrick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149

Immune Paralysis Induced by Pneumococcal Polysaceharide. I. Peritoneal Cell Phagocytic Ac- t ivity and Interaction with Spleen Cells. William G. Wu, Finn O. Horve and Carole C. Williams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154

Immune Paralysis Induced by Pneumocoecal Polysaccharide. II. Studies with Peritoneal and Spleen Cell Extracts and RNA. Carole C. WilliAms and William G. Wu . . . . . . . . . . . . . . . . . . . . 163

Secretion of Monospeeifie Anti-Hapten IgG Antibodies by Rabbit Immunoeytes. Edgar E. Hanna and Bruce Merchant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172

iii

iv CONTENTS [VOL. 107

Augmentation of PHA Responsiveness in Mixed Thymus-Marrow Cultures. Alan Winkelstein.. 195 Murine Malaria: The Role of Humoral Factors and Macrophages in Destruction of Parasitized

Erythrocytes. B. Sue Criswell, William T. Butler, Roger D. Rossen and Vernon Knight .... 212 Prediction of Immunosuppressive Potency of Antilymphocyte Serum by in Vitro Testing. P.G.

Gill, W. enables and J. Ludbrook ........................................................ 244 Immunologic Enhancement in Malaria Infection (Plasmodium b~rffhei). Christoph Jerusalem,

Margaret L. Weiss and Lambert Poels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 260 Distribution of Antigen Ribonucleoprotein Complexes within Rat Peritoneal Exudate Cells.

David C. Bishop and A. Arthur Gottlieb . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 269

IMMUNOPATHOLOGY

Detection of Antibodies to the Basic Protein of Human Myelin by Radiolmmunoassay and lrnmu- nofluorescence. Vanda A. Lennon, Senga Whittingham, Patrick R. Carnegie, Thomas A. McPherson and Inn R. Mackay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Studies on Antigen-Antibody Complexes. II. Quantification of Tissue Uptake of Soluble Complexes in Normal and Complement-Depleted Rabbits. William P. Arend and Mart Mannik . . . . . . . . 63

MICROBIAL AND VIRAL IMMUNOLOGY

Blocking Serum Lysis of Brucella aborSu8 by Hype~mmune Rabbit lmmunoglobulin A. Wendell H. Hall, Robert E. Manion and Horace H. Zinneman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Experimental Respiratory Syncytial Virus Infection of Adults. Possible Mechanisms of Resistance to Infection and Illness. John Mills V, John E. Van Kirk, Peter F. Wright and Robert M. Chanock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123

The Effect of Transplanted Lymphoid Cells, Irradiation, and Drug Therapy on Permanent Im- munologic Tolerance. JCrgen Hannover Larsen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185

Role of Macrophages and Antibody in Resistance of Mice against Yellow Fever Virus. B. Zisman, E. F. Wheelock and A. C. Allison . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 236

Immunoelectrophoretic Studies of the California Encephalitis Virus Group. F. M. WeUings, G. E. Sather and W. McD. H~mmon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 252

COMMUNICATIONS

PCA Reactions in Adult Specific Pathogen-Free Chickens. A. Conway Ettinger and A. A. Hirata. 278 Experimental Allergic Encephalomyelitis: Species Variability of the Encephalitogenic Determi-

nant. Robert H. Swanborg and Laurence S. Amesse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 281 Size and Density Analysis of Rodent Lymphoid Cells. Alan C. Aisenberg and Caroline Murray.. 284 The Thymus in the Chicken and Its Anatomical Relationship to the Thyroid. D. Panigraphi,

G. L. Waxier and V. H. Mailman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289 In Vitro Response of Human Leukocytes to Associated and Dissociated Hemocyanin. Alexander

A. Green and Luis Borella . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 293 The Unique Molecular Weight of the Heavy Chain from Human IgG3. Paul H. Saluk and L.

William Clem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 298 An in Vitro Immune Response to Penicillin. David Naor, Claudia Henry and H. Hugh Fudenberg. 302 Hapten-Carrier Relationships in the Production of Rat Homocytotropic Antibodies. Gill H.

Strejan and David G. Marsh . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 306

SPECIAL ARTICLE

Abstracts of Papers Presented at the Fourth International Complement Workshop, Johns Hopkins University School of Medicine, Baltimore, Maryland, January 27-29, 1971 . . . . . . . . . . . . . . . . . . . . 309

NUMBER 2, AUGUST 1971

IMMUNOCHEMISTRY

Limited Heterogeneity of Antibodies against the Para-Azobensoate (Xp) Hapten. Donald R. Hoffman, Allan L. Grossberg and David Pressman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 325

Effect of Cold Exposure on the Immunologic Response of Rabbits to Human Serum Albumin. J. E. M. St. Rose and B. H. Sabiston . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339

1971] CONTENTS v

Antigen Recognition and the Immune Response: The Capacity of L-Tyrosine-Azobenzenearsonate to Serve as a Carrier for a Macromolecular Hapten. Sefik S. Alkan, Danute E. Nitecki and Joel W. Goodman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 353

Lack of Antibody Activity in Human Myeloma Proteins. T . J . Yoo and Edward C. Franklin . . . . 365 Rabbit Secretory IgA. I. Isolation of Secretory Component after Selective Dissociation of the

Immunoglobulin. Jose A. O'Daly and John J. Cebra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 436 Rabbit Secretory IgA. II. Free Secretory Component from Colostrum and Its Specific Association

with IgA. Jose A. O'Daly and John J. Cebra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 449 Association of Polypeptides with Ribonucleic Acid. I. Effect of Charge of the Polypeptide and

Immune Status of Animals. Marianne L. Egan, Robert D. Smyth and Paul H. Maurer . . . . . . 540 Immunochemical Studies on Bovine Carboxypeptidases A. Kenneth Amiraian and Thomas H.

Plummer, Jr . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 547 Immunochemistry of the Cell-Wall Carbohydrate of Group L Hemolytic Streptococci. W.W.

Karakawa, J. E. Wagner and J. H. Pazur . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 554 Distribution of H-2 AUoantigenic Specificities on Radiolabeled Papain-Solubilized Antigen

Fragments. Susan E. Cullen and Stanley G. Nathenson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 563

IMMUNOGENETICS

Genetic Control of Immune Responsiveness to Limiting Doses of Proteins and Hapten Protein Conjugates in Guinea Pigs. Ira Green and Baruj Benacerraf . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 374

The PLL Gene and Histocompatibility Genotype in Inbred and Random-Bred Guinea Pigsl Leonard Ellman, Ira Green and Baruj Benacerraf . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 382

Recessive Inheritance of Rapid Anti-Allotype Antibody Production. Berenice Kindred and Eberhardt Weiler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389

An Efficient Method for Isolation of HL-A Antigens from Hematopoietie Cell Lines. Y. Miyakawa, N. Tanigaki, Y. Yagi and D. Pressman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 394

HL-A Antigens from Hematopoietic Cell Lines: Molecular Size and Eleetrophoretic Mobility. N. Tanigaki, Y. Miyakawa, Y. Yagi and D. Pressman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 402

The Immunologic Response to Xenografts. Recognition of Mouse H-2 Histocompatibility Antigens by the Rat. David H. Sachs, Henry J. Winn and Paul S. Russell . . . . . . . . . . . . . . . . . . . . . . . . . . . . 481

CELLULAR IMMUNOLOGY

Macrophage-Antigen Interaction: Uptake Metabolism and Immunogenicity of Foreign Albumin. Jon R. Schmidtke and Emil R. Unanue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331

Inability of the NZB/NZW F~ Thymus to Transfer Cyclophosphamide-Induced Tolerance to Sheep Erythroeytes. Michael E. Jacobs, Joyce Kay Gordon and Norman Talal . . . . . . . . . . . . . . . . . . . . 359

Surface Immunoglobulins on Mouse Lymphoid Cells. Arthur D. Bankhurst and Noel L. Warner.. 368 The Influence of Transfusions from Unrelated Donors upon Marrow Grafts between Histocom-

patible Canine Siblings. Rainer Storb, Robert H. Rudolph, Theodore C. Graham and E. Donnall Thomas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 409

The Third Cell Type Required for the Immune Response of Spleen Cells in Vitro. Humberto Cosenza, Lee D. Leserman and Donald A. Rowley . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 414

Morphologic Changes in Draining Lymph Nodes and in Lymphocyte Cultures after Sensitization with Complete or Incomplete Freund's Adjuvant. Correlation with Immunologic Events in Vivo and in Culture. Ann M. Dvorak, Robert C. Bast, Jr., and Harold F. Dvorak . . . . . . . . . . . 422

In Vitro Studies of the Response of Hamster Lymphoeytes to Phytohemagglutinin and Antigenic Stimulation. Gerald W. Fernald and Richard S. Metzgar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 456

The lmmunosuppreesive Effects of Rauscher Leukemia Virus upon Spleen Cells Cultured in Cell- Impermeable Diffusion Chambers. II. Effects Upon IgM and IgG Immunologic Memory and on Response to Phytohemagglutinin and Stimulation with Allogeneic Cells. Luis Borella . . . . 464

Role of the Bursa of Fabricius in IgG and IgM Production in the Chicken: Evidence for The Role of a Non-Bursal Site in the Development of Humoral Immunity. K. G. Lerner, B. Gliek and F. C. McDuffie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 493

Alterations in the Kinetics of Hemagglutinin Formation following Embryonic Bursectomy. Edward J. Moticka and Pierson J. Van Alten . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 512

Recovery of the Antigen Retention Mechanism after Sublethal X-Irradiation in Mice Receiving Cells from Various Sources. P. Nettesheim and Anna S. Hammous . . . . . . . . . . . . . . . . . . . . . . . . 518

vi CONTENTS [VOL. 107

Effect of Antimacrophage Serum on Hypersensitivity (Schistasama maz~so~i EGG) and Foreign Body (Divinyl-Benzene Copolymer Bead) Granulomas. D .L . Boros and K. S. Warren . . . . . . 534

Regulation of Homocytotropic Antibody Formation in the Rat. IV. Effects of Various Immuno- suppressive Drugs. Masaru Taniguchi and Tomio Tada . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 579

IMMUNOPATHOLOGY

Biopolymer Membrane: A Model System for the Study of The Neutrophilic Leukocyte Response To Immune Complexes. David Hawkins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 344

Streptococcal Related Glomerulonephritis. III . Glomerulonephritis in Rhesus Monkeys lmmuno- logically Induced Both Actively and Passively with a Soluble Fraction from Nephritogenic Streptococcal Protoplasmic Membranes. A.S. Markowitz, Donald Horn, C. Aseron, Robert Novak and H. A. Battifora . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 504

Tumor Immunity in Hamsters Immunized with Fetal Tissues. J .H . Coggin Jr., K. R. Ambrose, B. B. Bellomy and N. G. Anderson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 526

In Vitro Studies of the Human Response to Organ Allografts: Appearance and Detection of Circu- lating Activation Lymphocytes. Evan M. Hersh, William T. Butler, Roger D. Rossen, Robert O. Morgan and Wadi Suki . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 571

MICROBIAL AND VIRAL IMMUNOLOGY

The Role of Complement in the Phagoeytosis of Candida albica~ by Mouse Peripheral Blood Leukocytes. Remo Morelli and Leon T. Rosenberg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 476

COMMUNICATIONS

Inhibition of Histamine and SRS-A from Monkey Lung Tissue by Chlorophenesin. A. Malley and L. Baecher . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 586

Renal Tubular Disease and Autoantibodies against Tubular Basement Membrane Induced in Guinea Pigs. Raymond W. Steblay and Ulrich Rudofsky . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 589

The Tmmunosuppressive Effect of Rabbit Anti-Mouse Maerophage Serum. Abdallah M. Isa . . . . . 595 Long-Term Reconstitution of Burseetomized Chickens. W.A. Cain and L. A. David . . . . . . . . . . . . 598 Combined Effect of Phytohemaggiutinin and Antithymocyte Serum on Skin Xenograft Survival

in Mice. C . M . C . Chen, C. F. Chung and L. C. Teai . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 601 Localization of Tmmunoglobulins in Human Prostatic Tissue. R . J . Ablin, M. J. Gonder and

W. A. Soanes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 603 Method of Serum IgA Isolation. J. Mestecky, R. Kuihavy and F. W. Kraus . . . . . . . . . . . . . . . . . . . . . 605 A Simplified Method for Measuring H-m~n IgE. Ysolina M. Centifanto and Herbert E. Kaufman. 608 The Role of Cyclic AMP in the Cytolytic Activity of Lymphocytes. Christopher S. Henney and

Lawrence M. Lichtenstein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 610 A Rapid Method for Direct HL-A Typing of Cultured Lymphoid Cells. Soldano Ferrone, Michele

A. Pellegrino and Ralph A. Remfeld . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 613

N~ BZR 3, SEPTEMBmR 1971

Presidential Address. Byron H. WA~....An . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 617

IMMUNOCHEMISTRY

Characterization of Three Homogeneotm Canine Tmmunogiobulins. Arthur I. Hurvitz, J. Michael Kehoe and J. Donald Capra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 648

Isolation and Purification of a Peptido-Rhamnomannan from the Yeast Form of ~porothri= sch~wkii. Structural and lmmunochemical Studies. Kenneth O. Lloyd and Minda A. Bitoon. 663

Studies of the Mechanism of Binding of Chemically Modified Cytophilic Antibodies to Macro- phages. Susan G. Thrasher and Stanley Cohen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 672

Increased Complement Efficiency through Control of Ionic Strength and Temperature. William D. Linscott . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 698

Spontaneous Reoxidation of the Subunits of Immunoglobulin M (IgM). T . K . S . Mukkur and F. P. Tnman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 705

C3b Inactivator of Man. II. Fragmeata Produced by C3b Inactivator of Cleavage of Cell-bound or Fluid Phase C3b. Shaun Ruddy and K. Frank Austen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 742

1971] CONTENTS vii

Tmmunochemical Assay of Antilymphocyte Serum with Subcellular Fractions and Purified Mem- branes. Roland Von Fellenberg, Hans U. Briner and Elisabeth Guggisberg . . . . . . . . . . . . . . . . . . 751

Detection of Bound C3 by a New Immunochemical Method. Tibor Borsos and Edward J. Leonard. 766 The Nature of the Antibody in Iso-Tmmtme Anti-L Sera Affecting Active Potassium Transport in

LK Sheep Red Cells. J . J . Snyder, B. A. Rasmnsen and P. K. Lauf . . . . . . . . . . . . . . . . . . . . . . . 772 Isolation and Physico-Chernlcal Characterization of the "IgM-Like" Immunogiobulin from the

Stingray Dasl/a/is amer/cana. William H. Johnston, Jr., Ronald T. Acton, Peter F. Woin- heimer, William Niedermeier, E. Edward Evans, Rmm~ Shelton and J. Claude Bennett . . . . . . 782

Exposure of Hidden Antigenic Determinants in Human IgG by Digestion with Spleen Proteases. Kurt Fehr and Joseph LoSpalluto . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 814

Tmmunochemical Studies of Infections Mononucleosis. I. Isolation and Characterization of Hetero- phile Antigens from Hemoglobin-Free Stroma. Mary Ann Fletcher and Betty J. Woolfolk . . . . 842

Degradation of Human IgM by Pepsin: Characterization of a High Molecular Weight Fragment. Ralph E. Schroheuloher and J. Claude Bennett . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 870

Evolution of the Tmmune Response. X. Tmmunoglobulins of the Bowfin: Subunit Nature. G.W. Litman, D. Frommel, J. Finstad and R. A. Good . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88J,

IMMUNOGENETICS

Genetic Control of Immune Responsiveness to a Glutamic Acid, Alanine, Tyrosine Copolymer in Mice. I. Linkage of Responsiveness to H-2 Genotype. W . J . Martin, P. H. Maurer and B. Benacerraf . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 715

IgE Levels in Normal Infants and Mothers and Inheritance Hypothesis. Michael Bazaral, H. Alice Orgel and Robert N. Hamburger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 794

CELLULAR IMMUNOLOGY

Activity in Vitro of Lymphocytos and Macrophages in Delayed Hypersensitivity. S. B. Salvin, Stewart Sell and Jane Nishio . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 655

Augmented Anti-Silz Antibody Responses to an SzIx-Protein Conjugate. William E. Paul, David H. Katz and Baruj Benacerraf . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 685

Quantitation of Tmrnunoglobulin- and Virus-Producing Cells in Rats Infected with Moloney Leu- kemia Virus. Natalie E. Cremer, Dee O. N. Taylor and Edwin H. Lennette . . . . . . . . . . . . . . . 689

Characterization of the Antibody Response to Type I I I Pneumococcal Polysaccharide at the Cellular Level. III . Studies on the Average Avidity of the Antibody Produced by Specific Plaque-Forming Cells. Phillip J. Baker, Benjamin Prescott, Philip W. Stashak and Diana F. Amsbaugh . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7i9

Cell Separation on Affinity Columns: The Preparation of Pure Populations of Anti-Hapten Specific Lymphocytes. Leon Wofsy, John Kimura and Paolo Truffa-Bachi . . . . . . . . . . . . . . . . . . . . . . . . . . 725

Antigenic Competition between Mouse Thyroglobulin and Bovine Serum Albumin Demonstrated by Adoptive Transfer. Frank J. Twarog and Noel R. Rose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 738

Relative Absence of Circulating Antigen-Reactive Cells during Oral Tmmunization. Salmon S. Goldberg, Sumner C. Kraft, Raymond D. A. Peterson and Richard M. Rothberg . . . . . . . . . . . . . 757

Early and Enhanced Germinal Center Formation and Antibody Responses in Mice after Primary Stimulation with Antigen-Isologons Antibody Complexes as Compared with Antigen Alone. Jean Lalssue, Hans Cottier, Max W. Hess and Richard D. Stoner . . . . . . . . . . . . . . . . . . . . . . . . . . 822

IMMUNOPATHOLOGY

Characterization of lmmunoglobulin G Anti-Glomerular Basement Membrane Antibodies Eluted from Kidneys of Patients with Glomerulonephritis. II. IgG Subtypes and in Vitro Comple- ment Fixation. John J. McPhaul, Jr., and Frank J. Dixon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 678

Studies on the Immune Response to Penicillin and Cephalothln in Humans. I. Optimal Conditions for Titration of Hemagglutinating Penicillin and Cephalothin Antibodies. Peter Spath, George Garratty and Lawrence Petz . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 854

Studies on the Tmmune Response to Penicillin and Cephalothin in Humans. II. Immunohemato- logic Reactions to Cephalothin Administration. Peter Spath, George Garratty and Lawrence Petz . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 860

viii CONTENTS [COL. 107

MICROBIAL AND VIRAL IMMUNOLOGY

Production and Evaluation of a Formalin-Killed Chikungunya Vaccine. V. R. Harrison, K. H. Eckels, P. J. Bartelloni and C. Hampton . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 643

AntioNeuraminidase Antibody Response in Serum and Nasal Secretions following Intranasal or Subcutaneous Inactivated A2/Hong Kong/68 Influenza Virus Vaccine. David S. Fedson, Robert V. Fulk, Margret A. Huber, Margaret A. Reisberg and Julius A. Kasel . . . . . . . . . . . . . . . 730

Ir-munoprecipitin Reaction of Influenza Virus-Antibody Complex with Anti-IgG. Harry Daugharty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 802

Arthritis Associated with Induced Rubella Infection. Pearay L. Ogra and Kenneth Herd . . . . . . . . 810 Precipitating Antibody to Group A Streptococcal Polysaccharide in Humans. Robert A. Zim-

merman, Arthur H. Auernheimer and Angelo Taranta . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 832 Characterization of Precipitating Antibody in Equine Infectious Anemia. Hideo Nakajima,

Yu]i Kono and Chuzo Ushimi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 889

COMMUNICATIONS

Cell-Mediated Cytotoxicity: An Assay Using *~sI-Iododeoxyuridine-Labeled Target Cells. Alfred M. Cohen, James F. Burdick and Alfred S. Ketcham . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 895

The IgE-Mediated Release of an Eosinophil Leukocyte Chemotactic Factor from Human Lung. A. B. Kay and K. Frank Austen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 899

Cells Involved in the Immune Response. XXIV. The Demonstration of the Multiclonal Nature of the Antigen-Reactive Cell Population in the Bone Marrow of the Normal Adult Rabbit. Maxwell Richter and Nabih I. Abdou . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 903

Inhibition of Macrophage Migration: A Test System Using Human Monocytes. Leonard S. Gold- berg, James S. Louie and Mary H. Baker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 906

A Simplification of the Radioactive Antigen-Binding Test by a Double Label Technique. Emil C. Gotschlich . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 910

lmmunoglobulin D Concentration in Pregnant Women. David G. Klapper and Hiram W. Men- denhall . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 912

kntigenicity of Alum and Aqueous Adenovirus Hexon Antigen Vaccines in Man. J. A. Kasel, R. B. Couch and R. G. Douglas, Jr . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 916

Complement Utilization by Guinea Pig 71 and ~2 Immunoglobulins through the C3 Activator System. Ann L. Sandberg, Otto G5tze, Hans J. Mfiller-Eberhard and Abraham G. Osler . . . . 920

SPECIAL ARTICLES

Workshop on Myeloma Proteins, Homogeneous Antibodies and Autoimmunity . . . . . . . . . . . . . . . . . . 924

NUMBER 4, OCTOBER 1971

IMMUNOCHEMISTRY

The Lymphocytotoxie Reaction: The Mechanism of Rabbit Complement Action. S. Ferrone, N. R. Cooper, M. A. Pellegrino and R. A. Reisfeld . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 939

A Simple Method for Quantitation of Haptens Using " Indi rec t" Single Radial lmmunodiffusion. Wolfgang Richter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 948

Peptides from the Site of Rabbit Antibody: Anti-3-Azopyridine Antibody. Bernard Friedenson, Yasushi Takcda, Oliver A. Roholt and David Pressman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 957

Further Studies on Migration Inhibitory Factor (MIF): Evidence for Its Glycoprotein Nature. Heinz G. Remold and John R. David . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1090

Comparative Immunologic Studies on Fish and Invertebrate Collagens. W. Meigel, B. Pontz, R. Timpl, E. Hieber, A. Nordwig, C. Steffen and K. KQhn . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1146

The Carbohydrate Moieties of Mouse Immunoglobulins: Composition and Evidence against a Role in Transplacental Transport. Frederick Miller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1161

Univalent Arsanilic Acid Derivatives as Elicitors of Passive Cutaneous Anaphylaxis. Leon T. Rosenberg, Alfred A. Amkraut, Richard B. Corp and Sidney Raffel . . . . . . . . . . . . . . . . . . . . . . . . . . 1175

CELLULAR IMMUNOLOGY

Detection of Anti-Phage Antibody by Passive Hemagglutination. II. Early Antibodies Produced in Vivo or in Vitro. W.S. Walker, M. Fishman and F. L. Adler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 953

1971] CONTENTS ix

Response of Canine Fetuses and Neonates to Antigenic Stimulation. Mosh4 Shifrine, J. B. Smith, Marie S. Bulgin, B. J. Bryant, Yuan-Chang Zee and B. I. Osburn . . . . . . . . . . . . . . . . . . . . . . . . . . . . 965

Mechanisms of Passive Sensitization. II. Presence of Receptors for IgE on Monkey Mast Cells. Hisao Tomioka and Kimishige Ishizaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 971

The Use of Human F(ab~)2 Fragments as Blocking Agents in the Mixed Lymphocyte Reaction. M. O. Hornung, E. Layne and J. C. McDonald . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 979

The Isotopic Antiglobulin Assay: Application to the Study of Hybrid Cell Membranes. M.C . Rechsteiner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 985

Antibody Formation by Spleen Cells of Immunologically Tolerant Mice Stimulated with Antigen- Antibody Complexes. Claudio Intini, Diego Segre, Mariangela Segre and Walter L. Myers.. . 1014

Cyclic AMP and Immune Responses. I. Influence of Poly A.U and cAMP on Antibody Formation in Vitro. Masaaki Ishizuka, Werner Braun and Takao Matsumoto . . . . . . . . . . . . . . . . . . . . . . . . . 1027

Cyclic AMP and Immune Responses. II. Phosphodiesterase Inhibitors as Potentiators of Poly- nueleotide Effects on Antibody Formation. Werner Braun and Masaaki Ishizuka . . . . . . . . . . . 1036

Suppression of Antibody Synthesis. I. Evidence for a Circulating Inhibitor of Antibody Synthesis Demonstrable at the Cellular Level. Anthony S. Fauci and John S. Johnson . . . . . . . . . . . . . . . . 1052

Suppression of Antibody Synthesis. II. The Effect of Carrier-Specific Cells upon Haptenic Com- petition. Anthony S. Fauei and John S. Johnson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1057

Graft-vs-Host Inhibition. IV. Production of Allogeneic Radiation Chimeras Using Incubated Spleen and Liver Cell Mixtures. Mortimer M. Bortin, Alfred A. R]mm and Edward C. Saltz- stein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1063

Alterations in the Antibody Response to Bovine Serum Albumin by Capsular Polysaccharide of Klebsiella pneumoniae. Izumi Nakashima, Takashi Kobayashi and Nobuo Kato . . . . . . . . . . . . 1112

Regulation of Homocytotropic Antibody Formation in the Rat. V. Cell Cooperation in the Anti- Hapten Homocytotropie Antibody Response. Tomio Tada and Ko Okumura . . . . . . . . . . . . . . . . 1137

Antigen-Binding Cells. I. Their Identification and Role in the Immune Response. E .R. Unanue.. 1168

IMMUNOPATHOLOGY

Spontaneous Thyroiditis in the Obese Strain of Chickens. VI. Thyroxine-Binding Antibodies. Lars-~. Nilsson, Noel R. Rose and Ernest Witebsky . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 997

Hemolytic Anemia in Agammaglobulinemic Chickens: A Model of "Autoimmune" Disease in Im- mune Deficiency. Paul L. Weiden and R. Michael Blaese . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1004

The Immediate Allergic Response: In Vitro Separation of Antigen Activation, Decay and Hista- mine Release. Lawrence M. Lichtenstein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1122

The Immediate Allergic Response: In Vitro Action of Cyclic AMP-Active and Other Drugs on the Two Stages of Histamine Release. Lawrence M. Lichtenstein and Robert DeBernardo... : .. 1131

Immunologic Investigations of Naturally Occurring Canine Thyroiditis. Gerald J. Mizejewski, Jennifer Baron and Gerald Poissant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1152

MICROBIAL AND VIRAL IMMUNOLOGY

Delayed Hypersensitivity to Ribosomal Protein from BCG. L. Ortiz-Ortiz, E. B. Solarolo and L. F. Bojalil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1022

Procholeragenoid: An Aggregated Intermediate in the Formation of Choleragenoid. Richard A. Finkelstein, Koichiro Fuji ta and Joseph J. LoSpalluto . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1043

Immunity to Hemophilus infl~nzae Type b. I. The Isolation, and Some Physicoehemical Serologic and Biologie Properties of the Capsular Polysaccharide of Hemophilus influenzae Type b. Louis P. Rodrigues, Rachel Schneerson and John B. Robbins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1071

Immunity to Disease Caused by Hemophilus influenzae Type b. II. Specificity and Some Biologic Characteristics of "Natural ," Infection-Acquired, and Immunization-Induced Antibodies to the Capsular Polysaccharide of Hemaphilus influenzae Type b. Rachel Schneerson, Louis P. Rodrigues, J. C. Parke, Jr., and John B. Robbins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1081

&ssay of Australia Antigen and Antibody Employing Double-Antibody and Solid-Phase Radio- immunoassay Techniques and Comparison with the Passive Hemagglutination Methods. F. Blaine Hollinger, Vance Vorndam and Gordon R. Dreesman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1099

Local Immunologic Response to Immunization with Inactivated Newcastle Disease Virus. Ziebxiya Zakay-Rones, R. Levy and G. Spira . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1180

x CONTENTS [voL. 107

COMMUNICATIONS

Effects of Intraperitoneal Thymus Graft on Hemolytic Plaque Formation. ~blkRsu Takada, Yumiko Takada and Julian L. Ambrus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1185

Comparison of the Effect of Elastase on Human Secretory IgA and Serum IgA. Anne Tax and Leonhard Korngold . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1189

Spontaneous Renal Lesions and Glomerular Deposits of IgG and Complement in Guinea Pigs. Raymond W. Steblay and Ulrich Rudofsky . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1192

Removal of Neuraminic Acid from H-2 Alloantigeus without Effect on Antigenic Reactivity. Akira Shimada and Stanley G. Nathenson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1197

Functional Relationship of Factor B in the Properdin System to C3 Proactivator of Human Serum. Ira Goodkofsky and Irwin H. Lepow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1200

Antiserum to a Soluble Lymphoid Membrane Preparation Stimulates HnmRu Leukocyte DNA Synthesis. Albert B. Einstein, Jr., Dean L. Mann, Susan Ficker and William D. Terry . . . . . . . 1205

ErythroeyteoBound C5 and C6 in Autolmmune Hemolytic Anemia. Robert O. Kerr, Agustin P. Dalmasso and Manuel E. Kaplan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1209

Species Distribution of J Chain. P . P . Weinheimer, J. Mestecky and R. T. Acton . . . . . . . . . . . . . . . 1211

NU~B~.R 5, NOVm~BZR 1971

IMMUNOCHEMISTRY

Recoustitution from Subunits of the Hapten Binding Sites and Idiotypic Determinants of Mouse Anti-Phosphorylcholine Myeloma Proteins. Alan Sher, Edith Lord and Melvin Cohn . . . . . . . 1226

Purification of the First Component of Human Complement by Affinity Chromatography on Hu- man 7 Globulin Linked to Sepharose. David H. Bing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1243

Amino Acid Sequences of Free and Blocked N-Termini of Leopard Shark Immunoglobulins. G.G. B. Klaus, D. E. Nitecki and J. W. Goodman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1250

Dual Pathways of Complement Interaction with Guinea Pig lmmunoglobulins. Ann L. Sandberg and Abraham G. Osier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1268

Characterization of a Soluble Nuclear Ribonucleoprotein Antigen Reactive with SLE Sera. Martha Mattioli and Morris Reichlin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1281

Isolation of H-2 Alloantigens Solubilised by the Detergent NP-40. Benjamin D. Schwartz and Stanley G. Nathenson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1363

Isolation of 7S IgM and Kappa Chaius from the Surface Membrane of Ti~ue Culture Cells Derived from a Burkitt Lymphoma. Trond Eskeland and Eva Klein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1368

Cytophilic Binding of Antlgen-Conjugated Erythrocytes: A Sensitive A~ay. Susan G. Thrasher, Nadia Doroszczak and Stanley Cohen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1394

The Reactions of Pepsin and Pepsin-Trypsin Digestion Producta of Bovine Serum Albumin with Antisera from Rabbits Ingesting this Protein. Ruel N. Wright and Richard M. Rothberg . . . . 1410

Immunoglobulin Classes in Aquatic Mammals. Characterization by Serologic Cross-Reactivity, Molecular Size and Binding of Humsu Free Secretory Component. Donald R. Nash and Jean-Pierre Mach . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1424

Immunoglobulin E Antibodies in Guinea Pigs: Characterisation of Monomeric and Polymeric Components. Colin Dobson, John H. Rockey and E. J. L. Soulsby . . . . . . . . . . . . . . . . . . . . . . . . 1431

IMMUNOGENETICS

Inheritance and Strain Distribution of a Rat Immunoglobnlln Allotype. George A. Gutman and Irving L. Weissman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1390

CELLULAR IMMUNOIDGY

Antibody-Mediated Suppression of the Immune Respomm: Effect on the Development of Immu- nologic Memory. John W. Safford, Jr., and Sei Tokuda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1213

Direct Observations on the Effect of "Proliferation Inhibitory Factor" on the Clonal Growth of Target Cells. Alison M. Badger, Sidney R. Cooperband and Jon A. Green . . . . . . . . . . . . . . . . . . 1259

Antibody Response in Genetically Thymus-less Nude Mice Inieeted with Normal Thymus Cells. Berenice Kindred . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1291

A Factor in Maternal Plasma during Pregnancy that Suppre~es the Reactivity of Mixed Leuko- cyte Culture. Shinpei Kasakura . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1296

1971] CONTENTS xi

Studies on Rabbit Lymphocytes in Vitro. XIV. Fractionation of Rabbit Peripheral Blood Lympho- cytes by Antibody-Coated Polyacrylamide Beads. Stewart Sell and Telsa An . . . . . . . . . . . . . . . . 1302

Effect of Blocking Cell Receptors on An Immune Response Resulting from in Vitro Cooperation between Thymocytes and Thymus-Independent Cells. G. Doria, G. Agarossi and S. Di Pietro. 1314

Carrier Function in Anti-Hapten Antibody Responses. V. Analysis of Cellular Events in the En- hancement of Antibody Responses by the "Allogeneic Effect" in DNP-OVA-Prhned Guinea Pigs Challenged with a Heterologous DNP-Conjugate. David H. Katz, William E. Paul and Baruj Benacerraf . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1319

Fate and Immunogenicity of Antigens Endocytosed by Macrophages: A Study Using Foreign Red Cells and lmmunoglobulin G. Andre Cruchaud and Emil R. Unanue . . . . . . . . . . . . . . . . . . . . . . . 1329

Effect of Antigen Variation on Production of Antibody in Canine Tracheal Secretions. Phillip Lieberman, Roy Patterson, erlyn Petersen, Lawrence W. Chakrln, Joe R. Wardell, Jr. and John Ricks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1349

Effect of Age upon Primary Alloantigen Recognition by Mouse Spleen Cells. William H. Adler, Tomoo Taldguchi and Richard T. Smith . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1357

Immunobiology of Human Lymphoid Cell Lines. I. Immunoglobulin Biosynthesis in Cultures from Hypogammaglobulinemias and Paraproteinemias. Daniel P. Stites, Alan S. Levin, Kay E. Austin and H. Hugh Fudenberg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1376

Transformation of Lymphocytes from Animals Sensitized to Trichinella spiralis. C . W . Kim, M. P. Jamuar and L. D. Hamilton . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1382

Bone Marrow-Derived Cells as Target Cells for Polynueleotide Adiuvants. P . A . Campbell and P. Kind . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1419

Antibody Production in Normal Children Receiving Penicillin at Birth. Michael J. Fellner, Maria V. Klaus, Rudolf L. Baer and Aaron R. Rausen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1440

IMMUNOPATHOLOGY

Acute Viral Infection: Tissue Injury Mediated by Anti-Viral Antibody Through a Complement Effector System. Michael B. A. Oldstone and Frank J. Dixon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1274

Induction of Thyroiditis in the Rabbit by Intravenous Injection of Papain-Treated Rabbit Thyro- globulin. Charles L. Anderson and Noel R. Rose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1341

MICROBIAL AND VIRAL IMMUNOLOGY

Herpesvirus Neutralization: The Role of Complement. Craig Wallis and Joseph L. Melnick . . . . . 1235 lmmunosuppression by Leukemia Viruses. VII . Stimulatory Effects of Friend Leukemia Virus on

Pre-Existing Antibody-Forming Ceils to Sheep Erythrocytes and Eschcrichia coli in Non- Immunized Mice. Shinji Hirano, Herman Friedman and Walter S. Ceglowski . . . . . . . . . . . . . . . 1400

Effect of Selected Lipids and Suffactants on lmmunogenicity of Several Bacterial Antigens. Man- jul K. Agarwal and E. Neter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1448

Immune Fever in the Rabbit: Responses of the Hematologic and Complement Systems. Ira D. Mickenberg, Ralph Snyderman, Richard K. Root, Stephen E. Mergenhagen and Sheldon M. Wolff . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1457

The Relationship of Complement Consumption to Immune Fever. Ira D. Mickenberg, Ralph Snyderman, Richard K. Root, Stephen E. Mergenhagen and Sheldon M. Wolff . . . . . . . . . . . . . . . 1466

COMMUNICATIONS

Schistosomal Egg Antigen-induced Lymphocyte Blastogenesis in Experimental Murine Echisto- soma man~oni Infection. Daniel G. Colley . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1477

Aleutian Disease of Mink: Detection of Large Quantities of Complement-Fixing Antibody to Viral Antigen. T .C . McGuire, T. B. Crawford, J. B. Heuson and J. R. Gorham . . . . . . . . . . . . . . . . . . 1481

Release of Macrophage Migration Inhibitory Factor by Virus-Infected Spleen Cells. David G. Tubergen and Michael B. A. Oldstone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1483

7S IglVI and IgA Influenza Fluorescent Antibodies in Serum. Gordon C. Brown and Theresa P. O'Leary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1486

Inhibition of Lymphocyte Stimulation by Cyclic and Non-Cyclic Nudeotides. Gene Estes, Solo- mon S. Solomon and Walter L. Norton . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1489

Enhancement of Hemolytic Complement Activity by Treatment of Human Serum with Iodine. Margaret J. Policy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1493

xii CONTENTS (voL. 107

Neoantigenic Expressions Engendered by Plasmin Cleavage of Fibrinogen. Edward F. Plow, Cecil Hougie and Thomas S. Edgington . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1496

Membrane Immunoglobulins of Lymphocytes in NZB Mice. Nirmala Bhoopalam, ~'incent J. Yakulis, Nicholas Costea and Paul Heller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1501

NUMBER 6, D]~C~]31R 1971

IMMUNOCHEMISTRY

Common Structural and Antigenic Properties of Human "rM Anti-~ Globulins. Edward C. Frank- lin and Blas Frangione . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1527

The Specificity and Cross-Reactivity of Antisynaptosome Antibodies as Determined by Sequential Adsorption Analysis. Don D. Mickey, Paul N. McMillan, Stanley H. Appel and Eugene D. Day . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1599

The Specificity and Cross-Reactivity of Antimyelin Antibodies as Determined by Sequential Ad- sorption Analysis. Paul N. McMillan, Don D. Mickey, Bernard Kaufman and Eugene D. Day . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1611

Immunoglobulins in Intact, Immunized, and Contaminated Axenic Mice: Study of Serum IgA. J. Beneveniste, G. Lespinats, C. Adam and J.-C. Salomon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1647

Serum and Secretory IgA in Axenic and Holoxenic Mice. Jacques Benveniste, Genevieve Lespi- nats and Jean-Claude Salomon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1656

Isolation of the Anticomplementary Protein from Cobra Venom and Its Mode of Action on C3. Hans J. Miiller-Eberhard and Karl-Erik FjellstrSm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1666

Bence Jones Proteins and Light Chains of Immunoglobulins. VI. lmm~unochemical Detection of Interclass Similarities among ~-Chains. Carla L. McLaughlin and Alan Solomon . . . . . . . . . . . 1699

The Use of Polyacrylamide Gel as an Tmmunoadsorbent for Anti-2,4-Dinitrophenyl Antibodies. H. Jaquet and H. Francis Havas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1729

Immunogenic Specificity of Baci l lus subt i l i s Phage SPO1-DNA. N.A. Braude and L. A. Zamchuk. 1766

IMMUNOGENETICS

Relations of the Human Antibody Response to Flagellin to Gm Genotype. J. Vivian Wells, H. Hugh Fudenberg and Ian R. MacKay ..................................................... 1505

CELLULAR IMMUNOLOGY

The Importance of 0 and Ig Bearing Cells in the Immune Response to Various Antigens. T. Taka- hashi, J. J. Mond, E. A. Carswell and G. J. Thorbecke . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1520

Quantitation of the Cell-Mediated Immune Response. I. The Number of Cytolytically Active Mouse Lymphoid Cells Induced by Immunization with Allogeneic Mastocytoma Cells. Chris- topher S. Henney . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1558

Regulation of Antibody Response i n Vi t ro . I. Suppression of Secondary Response by Anti-Immuno- globulin Heavy Chains. Tadamitsu Kishimoto and Kimiahige Ishizaka . . . . . . . . . . . . . . . . . . . . 1567

Role of Basophilic Leukocytes in Cellular Immunity to Vacclnia Virus Infection. Harold F. Dvorak and Martin S. Hirsch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1576

Cell Surface Associated Gamma Globulins in Lymphocytes. II. The Pluripotentiality of Mouse Spleen Lymphocytes. S.-T. Lee, F. Paraskevas and L. G. Israels . . . . . . . . . . . . . . . . . . . . . . . . . . 1583

Immunoglobulin (Ig) Receptors on Human Peripheral Leukocytes. II. Class Restriction of Ig Receptors. Nabih I. Abdou . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1637

The Effect of Excision of the Site of Application on the Induction of Delayed Contact Sensitivity. H. P. Godfrey and Harold Baer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1643

Antigen Binding Cells. II. Effect of Highly Radioactive Antigen on the Immunologic Function of Bone Marrow Cells. E . R . Unanue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1663

Regulation of Homocytotropic Antibody Formation in the Rat. VI. Inhibitory Effect of Thymo- eytes on the Homocytotropic Antibody Response. Ko Okumura and Tomio Tada . . . . . . . . . . 1682

Antibody against Neoplastic Plasma Cells. II. Suppressive Effect on Antibody-Producing Cells. Takeshi Watanabe, Yasuo Yagi and David Pressman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1706

The DNA Synthetic Response of Adoptively Transferred Thymocytes in the Spleens of Lethally Irradiated Mice. I. Effects of Varying Antigen and Thymocyte Doses. Richard K. Gershon and Ronald S. Hencin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1723

1971] CONTENTS xiii

Suppression of in Vitro Antigen-Induced Lymphocyte Transformation by Carrageenan, A Macro- phage-Toxic Agent. W. Wayne Lake, David Bice, Howard J. Schwartz and John Salvaggio 1745

Cellular Formation of Vibriolytic Antibody by Mouse Immunocytes: Cytokinetics and Specificity. Robert F. McAlack, Jan Cerny and Herman Friedman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1752

IMMUNOPATHOLOGY

The Preparation of Human Kininogen. II. Further Characterization of Purified Human Kininogen. Jocelyn Spragg and K. Frank Austen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1512

The Immunologic Release of Constituents from Neutrophil Leukocytes. I. The Role of Antibody and Complement on Nonphagoeytosable Surfaces or Phagocytosable Particles. Peter M. Henson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1535

The Immunologic Release of Constituents from Neutrophil Leukocytes. II. Mechanisms of Re- lease During Phagocytosis, and Adherence to Nonphagocytosable Surfaces. Peter M. Henson. 1547

Murine Amyloidosis: Immunologic Characterization of Amyloid Fibril Protein. Chaviva Isersky, David L. Page, Pedro Cuatrecasas, Ronald A. DeLellis and George G. Glenner . . . . . . . . : .... 1690

Immune Adherence Reactions of Human Erythrocytes Sensitized with Complement in Vitro and in Vivo. Lawrence Petz, H. Hugh Fudenberg and Diane Fink . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1714

The Complement-Fixation Reaction in Equine Infectious Anemia: Demonstration of Inhibition by IgG(T). T . C . McGuire, G. L. Van Hossier, Jr., and J. B. Henson . . . . . . . . . . . . . . . . . . . . . 1738

MICROBIAL AND VIRAL IMMUNOLOGY

Latent Measles Virus Infection of the Hamster Central Nervous System. Douglas J. Wear and Fred Rapp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1593

Complement-Requiring Neutralizing Antibodies in Hyperimmune Sera to Human Cytomegalo- viruses. Bettie J. Graham, Yoichi Minamishima, Gordon R. Dreesman, Harold G. Haines and Matilda Benyesh-Melnick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1618

Anthrax Indirect Microhemagglutination Test. Thomas M. Buchanan, James C. Feeley, Peggy S. Hayes and Philip S. Brachman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1631

Leukemia Virus-Induced lmmunosuppression. VIII. Rapid Depression of in Vitro Leukocyte Migration after Infection of Mice with Friend Leukemia Virus. Herman Friedman and Walter S. CegIowski . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . : . . . . . . . . . . . . . . . . 1673

COMMUNICATIONS

Evolution of Delayed Hypersensitivity Reactions in Man. Michael W. Brandriss . . . . . . . . . . . . . . 1771 Immune Response in Vitro to Salmonella H-Antigens, Not Affected by Anti-Theta Serum. E.

Diener, Frances O'Caltaghan and N. Kraft . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1775 Potentiation of Cultured Mouse Thymocyte Responses by Factors Released by Peripheral Leu-

cocytes. I. Gery, R. K. Gershon and B. H. Waksman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1778 Amino Terminal Sequences of Heavy and Light Chains of Chicken Anti-Dinitrophenyl Antibody.

Ralph Kubo, Irwin Y. Rosenblum and Albert A. Benedict . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1781 A Polypeptide Chain from Leopard Shark 19S Immunogiobulin Analogous to Mammalian J Chain.

G. G. B. Klaus, M. S. Halpern, M. E. Koshland and J. W. Goodman . . . . . . . . . . . . . . . . . . . . . . . 1785 Noncovalent Interaction between Immunoglobulin (Ig) Polypeptide Chains. II. Absence of Hy-

brid Molecule Formation between Ig Classes. Barry Zimmerman and Howard M. Grey . . . . . . 1788 Lymphoproliferative Cycles in the Thymus Cortex. B . J . Bryant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1791 Immunoglobulin Receptors on Mouse Mast Cells. Mast Cells of Different Strains and Mastocy-

tomas. Zoltan Ovary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1795

BIOLOGICAL STANDARDIZATION

Measurements of Concentrations of Human Serum lmmunoglobulins . . . . . . . . . . . . . . . . . . . . . . . . . . . 1798

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Medical and Veterinary

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This book is designed to assist the medical and veterinary student and practi- tioner to identify the protozoa which cause disease in man and in domestic animals. It is intended to be complementary to the various textbooks avail- able. Experience has shown that diagrams are of limited value in helping students to recognize the various protozoa. The outstanding feature of this book is the comprehensive depiction of the protozoan parasites in color photography.

1971/208 pp./186 figs./$19.75

Infection in Hospital A Code of Practice

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This book brings together the personal experience of a number of people in various branches of medicine, concerned in the control of infection in hospital and in the community. Simple guidance on the treatment and how best to avoid infection arising and spreading is also provided.

1971/101 pp./$6.75

Tropical Diseases 4th Edition

By Frederick Wright, M.A., M.D., F.R.C.P., D.T.M.&H., Senior Lecturer in Diseases of Tropical Climates, University of Edinburgh, and James P. Baird, M.D., F.R.C.P., recently Professor of Military Medicine of the Royal Army Medical College

This book has proved a valuable supplement to Sir Stanley Davidson's "Principles and Practice of Medicine". The conditions covered are grouped largely on an aetiological basis, beginning with important protozoal infec- tions not included in the classic text. Considerable progress has been made in tropical medicine since the printing of the third edition and every endeavour has been made to bring the text up to date by appropriate deletions and additions.

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THE JOURNAL OF IMMUNOLOGY Information for Subscribers

Published monthly, beginning January, 1929. (From the 1916-1925, and in 1928 Volumes I-X inclusive, and Vol. XV published bi-monthly.)

The subscription price is $48.00 per year in U.S.A.; $51.00 elsewhere. Single copies, when available, are $5.00 in U.S.A.; $5.75 elsewhere. The subscriptions of members of the American Association of Immunologists are included in the membership dues and are handled by the Association's Treasurer, Dr. Henry Metzger, American Association of Immunologists, 9650 Roekville Pike, Bethe.sda, Md. 20014. All other subscriptions should be sent to the publishers.

Change of address: Publisher must be notified 60 days in advance. Journals undeliverable be- cause of incorrect address will be destroyed. Duplicates can be obtained (if available) from the publisher at the regular price of single issues.

New subscriptions and renewals are entered to begin with the issue scheduled for publica- tion 60 days after receipt of the order, unless otherwise requested.

Subscriptions should be renewed promptly: To avoid a break in your series, subscriptions should be renewed promptly. The publishers cannot guarantee to supply back issues on belated renewals.

Back volumes are available in microfilm from University Microfilm, Ann Arbor, Michigan 48106, and Arcata Microfilm Corp., Spring Valley, New York 10977.

Reprints of individual articles are available only from authors.

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Clinical and Experimental Immunology A n Official J o u r n a l o f

T H E B R I T I S H S O C I E T Y F O R I M M U N O L O G Y

Volume 8 Number 3 March 1971

CONTENTS

J. L. TURK AND M. F. R. WATERS: Immunological significance of changes in lymph nodes across the leprosy spectrum

A. E. G. KR. YON DEM BORNE, C. P. ENGELFRIET, DO BECKERS AND J. J. VAN LOOHEM: Autoimmune haemolytic anaemias. IV. Biochemical studies of red cells from patients with autoimmune haemolytic anaemia with incomplete warm autoantibodies

K. PENTTINEN, A. VAHERI AND G. MYLLYLA. Detection and characterization of immune complexes by the platelet aggregation test. I . Complexes formed in vitro

G. MYLLYI~, A. VAHERI AND K. PETTINEN: Detection and characterization of immune complexes by the platelet aggregation test. II. Circulating complexes

S. E. SALMON, VERA B. MORHENN AND M. J. CLINK: Uptake of radioiodinated antigens by human monocytes

MARGARET G. FITZGERALD: The establishment of a normal human population dose-response curve for lymphocytes cultured with PHA (phytohaemaggiu- tinin)

J. AYOUB AND S. KASAKURA : In v/tro response of foetal lymphocytes to PHA, and a factor plasma which suppreses the PHA response of adult lymphocytes

MARTIIA M. EIBL: Rosette formation by sensitized human erythrocytes on various heterologous macrophages

P. BRAIN AND JUNE GORDON: Rosette formation by peripheral lymphocytes. II. Inhibition of the phenomenon

MARION WALLER, R. J. DUMA, E. D. FARLEY, JR., AND JANE ATKINSON: The in- fluence of infection on titres of antiglobulin antibodies

H. A. WARD, R. C. NA.IRN AND W. G. R. M. DE BOER: Heterologous antiserum against gastric parietal cells induced by sub-microsomal gastric mueosal fraction

B. M. GREENWOOD, J. H. L. PLAYFAIR AND G. TORRIGIANI: Immunosuppression in marine malaria. I. General characteristics

A. CHAFFER AND J. H. L. PLAYFAIR: The genetic basis of autoimmunity in NZB mice studied by progeny testing

MARION M. ZATZ, R. C. MELLORS AND E. M.. LANCE : Changes in lymphoid popula- tions of ageing CBA and NZB mice

A. F. GAZDAR, W. BEITZEL AND N. TALAL: The age related responses of New Zealand mice to marine sarcoma virus

UNO AXELSSON AND ANNA-BRITA LAURELL: A case of angioneurotic oedema with a high content of non-functioning, double peaked C1 esterase inhibitor

The subscription price in lhe Uniled States and Canada is $80.00 for lhe two annual volumes of six parts (Mngles copies 87.50 each)

BLACKWELL SCIENTIF IC PUBLICATIONS LTD O X F O R D AND EDINBURGH

THIB JOUgWtT. OP ~ o z , o a r VoL 107, No . 6, D e e e m b e r 1971 Copyright ~ 1971 by The Willimrrm & W|llrln. CO. V ~ in U.8,A.

Announcement

SURVEY COURSE IN I M M U N O L O G Y

The Annual Survey Course in Immunology, sponsored by the American Association of Immunologists, will be held at the University of California at San Diego (UCSD) in La Jolla, California, July 17 through July 28, 1972.

The course is intended primarily for university or college instructors. The number to be selected will be limited to approximately 50. Selection will be on a competitive basis and decided by the Committee on Admissions.

Applicants should submit a letter to Elmer L. Beaker, M.D., Professor of Pathology, University of Connecticut Health Center, Farmington, Connecticut 06032, not later than April 1, 1972. The letter should be accompanied with the following information and supplied in quedmplicate: 1) curriculum vitae, 2) current research and teaching activities, 3) masons for wanting to enroll in the course, and 4) background in immunology.

There are no formal application bl~nks. A $100 registration fee will be charged. Per diem costs at the University are approximately $10.