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High Resolution in Mass and Space: AP-MALDI Imaging using...
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laser beam path = ion trajectory = observation path = base plate = CCD Camera mirror (centrally bored) objective lens (centrally bored) movable sample stage (x, y, z) observation mirror (off axis) aperture lens mirror static and variable attenuator N 2 Laser Thermo Scientific Q Exactive TM mirror Performance of AP-SMALDI MSImaging imagine10 Spatial Resolution Conclusion The methods applied to Orbitrap-based instrumentation shows high reliability, validity and performance for routine histological analysis. Imaging selectivity, dynamic range are significantly improved upon combining high spatial resolution (here down to 3 μm, typically 10 um) with high mass resolution and high mass accuracy obtained with Orbitrap-based instrumentation. Such highly spatially resolved, high mass resolution MS/MS data are shown for the first time. References [1] Spengler B, Hubert M, Kaufmann R, MALDI Ion Imaging and Biological Ion Imaging with a new Scanning UV-Laser Microprobe, Proceedings of the 42nd Annual Conference on Mass Spectrometry and Allied Topics, Chicago, IL, May 29 - June 3, 1994, pp 1041. [2] Römpp, A., S. Guenther, Y. Schober, O. Schulz, Z. Takats, W. Kummer, and B. Spengler (2010), Histology by Mass Spectrometry: Label-Free Tissue Characterization Obtained from High-Accuracy Bioanalytical Imaging. Angewandte Chemie International Edition. 49(22): p. 3834-3838. [3] Guenther, S., Römpp, A., Kummer, W., Spengler B. (2011), AP-MALDI Imaging of neuropeptides in mouse pituitary gland with 5 μm spatial resolution and high mass accuracy. Int. J. Mass Spectrom. 305, 228-237. [4] Römpp, A., Guenther, S., Takats, Z., Spengler, B. (2011), Mass spectrometry imaging with high resolution in mass and space (HR² MSI) for reliable investigation of drug compound distributions on the cellular level. Analytical and Bioanalytical Chemistry 401, 65-73. [5] Schober, Y., Schramm, T., Spengler, B., Römpp, A. (2011), Protein identification by accurate mass MALDI imaging of tryptic peptides. Rapid Communications in Mass Spectrometry 25, 2475-2483. Acknowledgements The authors acknowledge financial support by the Hessian Ministry of Science and Arts (HMWK) through LOEWE focus “AmbiProbe“. imagine10 is trademark of TransMIT GmbH, Giessen, Germany. Orbitrap, Exactive and Q Exactive are trademarks of Thermo Fisher Scientific and its subsidiaries. Not all products are available in all countries. This information is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others. Presented at ASMS2012 (T436) Vancouver, Canada Overview Purpose: Short synopsis about the research approach. Methods: Short synopsis of the experimental methods. Results: Short synopsis of the results. Introduction MALDI Mass Spectrometry Imaging (MSI), 17 years after its first announcement [1], has turned into a routine method of highest performance for the molecular histology of biological tissue [2]. The method, providing high resolution and accuracy in mass and space, has been developed for a reliable identification and localization of individual tissue components, and has been applied recently to a number of research areas in pathology, cancer diagnostics, metabolic pathway analysis and plant research [2-5]. Targeted compound classes included phospholipids, peptides, proteins, drug compounds and metabolites. An atmospheric pressure (AP) ion source has been developed for these studies, based on a dedicated microoptical setup that provides highest spatial resolution of 3 to 5 micrometer on tissue in imaging mode on Orbitrap-based instrumentation. The advantages of Orbitrap detection are given here: 1. High mass resolution allows to analyze complex tissue samples, sample complexity 2. High mass accuracy enables for straightforward sum formula assignments and facilitates substance identification, substance identification Accurate MS and MS/MS imaging data allow to align images, confirmation 3. Irrelevance of sample morphology/flatness, ease of sample / matrix preparation 4. Convenience of AP interface, compatibility to ambient analysis High Resolution in Mass and Space: AP-MALDI Imaging using Orbitrap-based Instrumentation Bernhard Spengler 1,3 , Andreas Römpp 1 , Sabine Günther 1 , Oliver Schulz 1 , Klaus-Peter Hinz 1 , Alfons Hester 1 , Christian Schinz 1 , Christian Lotze 1 , Jörg-Ulrich Pötzl 1 , Kerstin Strupat 2 1 Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Germany * [email protected] 2 Thermo Fisher Scientific (Bremen) GmbH, Germany, [email protected], 3 TransMIT GmbH, Giessen, Germany, [email protected] Open-cover view of the atmospheric pressure imaging ion source imagine10 (TransMIT GmbH, Giessen) attached to a Thermo Scientific Q Exactive TM Orbitrap instrument, Thermo Fisher Scientific (Bremen) GmbH Scheme of the atmospheric pressure imaging ion source imagine10 (TransMIT GmbH, Giessen) attached to a Thermo Scientific Q Exactive TM Orbitrap instrument (Thermo Fisher Scientific (Bremen) GmbH) Coronal mouse brain section 35 μm steps, 295 x 195 pixels Left: Focus size measurements (most left) and ablation on tissue reveal an effective pixel resolution in the range of 5 μm, effective pixel size if laser irradiance dependent. Above: Un-blurred red/green pixels indicate an effective spatial resolution on mouse brain tissue (lateral ventricle) of 3 μm. Left: Single pixel (3 μm) = single scan Exactive MS Orbitrap mass spectrum Lateral ventricle, 3 μm steps, 170 x 200 pixels Experimental RMS = 0.2 ppm +/- 1 ppm 30,000 consecutive scans of PC(34:1) at m/z = 798.5410 on tissue during image acquisiton using internal lock mass operation. Representative single pixel (10 x 10 μm 2 ) mass spectrum upon automated internal lock mass operation. imagine10 source attached to Q Exactive Orbitrap instrument MS and MS/MS Imaging A coaxial ion source geometry was employed to irradiate, desorb, ionize and transport biological compounds under atmospheric pressure conditions. Ions are introduced into the mass spectrometer through an extended heated capillary. Transfer efficiency was optimized using both, gas stream dynamics and electrostatic ion focusing. The achievable mass accuracy during tissue imaging operation is typically better than 1 ppm RMS using lock mass calibration. Imaging speed is up to 2 pixels per second, depending on mass range and mass resolution setting. Matrix preparation and on-tissue protein digestion were performed using a new dedicated protocol. 50 μm 100 μm Red: background Green: PC(38:4) Blue: PC(34:1) Healthy mouse brain tissue. Two phospholipids differing by 8 mmu (m/z 848.55 and m/z 848.63) are extracted into an MSimage. 10 μm, 400 x 400 pixels (= 4*4 mm 2 ) Mass Resolution = 70000 @ m/z 400 imagine10 source attached to Q Exactive Orbitrap instrumentation T007 CK75 MS-MSMS772 200x200 10um E17 # 37003 RT: 805.25 AV: 1 NL: 9.03E4 T: FTMS + p NSI Full ms [700.00-900.00] 700 720 740 760 780 800 820 840 860 880 900 m/z 0 10 20 30 40 50 60 70 80 90 100 Relative Abundance 798.55066 772.53479 896.61188 782.57678 826.58234 870.59595 716.13373 760.59467 734.57874 848.56702 820.53528 876.59943 S194 CK3 170x200 3um E170_RC798 #26687 RT: 548.39 AV: 1 NL: 6.75E3 FTMS + p NSI Full ms [100.00-1000.00] 700 720 740 760 780 800 820 840 860 880 900 m/z 0 50 100 Relative Abundance 798.5412 772.5258 896.6012 769.5638 782.5681 870.5860 739.4668 848.5556 713.4514 826.5722 756.5528 844.5272 804.5507 893.4771 Mass Resolution on Tissue Mass Selectivity & Dynamic Range Closely neighbored signals, even of drastically differing peak intensities, can be clearly distiguished and discriminated. Bin width Δm/z was 0.01 u. 798.5416 PC(34:1)+K + 798.5909 PE(38:0)+Na + 797 798 799 800 801 802 798.5416 799.5453 800.5596 801.5636 798.45 798.50 798.55 798.60 m/z 0.0 0.2 0.4 0.6 0.8 1.0 1.2 Relative Abundance 798.5990 798.4895 12.5 ppm window (Δm/z=0.01) m/z Healthy mouse brain tissue, 10 um step size, 200x200 pixels (= 2 *2 mm 2 ). MS and MS/MS scans on adjacent, neigboring pixels. Left: m/z 772 (MS Orbitrap, full scan) right: m/z 713 (MSMS of 772), loss of trimethylamine (59 u). MS/MS experiment confirms the phospholipid classification as phosphatidylcholine or shingomyeline. imagine10 source attached to Q Exactive Orbitrap instrument. m/z 772.523 m/z 713.450
Transcript of High Resolution in Mass and Space: AP-MALDI Imaging using...
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laser beam path = ion trajectory =observation path =base plate =
CCD Camera
mirror(centrally bored)
objective lens(centrally bored)
movable sample stage (x, y, z)
observation mirror(off axis)
aperture
lens mirror
static and variable attenuator
N2 Laser
Thermo ScientificQ ExactiveTM
mirror
Performance of AP-SMALDI MSImaging imagine10Spatial Resolution
ConclusionThe methods applied to Orbitrap-based instrumentation shows high reliability, validity and performance for routine histological analysis.
Imaging selectivity, dynamic range are significantly improved upon combining high spatial resolution (here down to 3 µm, typically 10 um) with high mass resolution and high mass accuracy obtained with Orbitrap-based instrumentation.
Such highly spatially resolved, high mass resolution MS/MS data are shown for the first time.
References[1] Spengler B, Hubert M, Kaufmann R, MALDI Ion Imaging and Biological Ion Imaging with a new Scanning UV-Laser
Microprobe, Proceedings of the 42nd Annual Conference on Mass Spectrometry and Allied Topics, Chicago, IL, May 29 - June 3, 1994, pp 1041.
[2] Römpp, A., S. Guenther, Y. Schober, O. Schulz, Z. Takats, W. Kummer, and B. Spengler (2010), Histology by Mass Spectrometry: Label-Free Tissue Characterization Obtained from High-Accuracy Bioanalytical Imaging. Angewandte ChemieInternational Edition. 49(22): p. 3834-3838.
[3] Guenther, S., Römpp, A., Kummer, W., Spengler B. (2011), AP-MALDI Imaging of neuropeptides in mouse pituitary gland with 5 µm spatial resolution and high mass accuracy. Int. J. Mass Spectrom. 305, 228-237.
[4] Römpp, A., Guenther, S., Takats, Z., Spengler, B. (2011), Mass spectrometry imaging with high resolution in mass and space (HR² MSI) for reliable investigation of drug compound distributions on the cellular level. Analytical and Bioanalytical Chemistry 401, 65-73.
[5] Schober, Y., Schramm, T., Spengler, B., Römpp, A. (2011), Protein identification by accurate mass MALDI imaging of trypticpeptides. Rapid Communications in Mass Spectrometry 25, 2475-2483.
AcknowledgementsThe authors acknowledge financial support by the Hessian Ministry of Science and Arts (HMWK) through LOEWE focus “AmbiProbe“.
imagine10 is trademark of TransMIT GmbH, Giessen, Germany. Orbitrap, Exactive and Q Exactive are trademarks of Thermo Fisher Scientific and its subsidiaries. Not all products are available in all countries. This information is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others. Presented at ASMS2012 (T436) Vancouver, Canada
OverviewPurpose: Short synopsis about the research approach.
Methods: Short synopsis of the experimental methods.
Results: Short synopsis of the results.
IntroductionMALDI Mass Spectrometry Imaging (MSI), 17 years after its first announcement [1], has turned into a routine method of highest performance for the molecular histology of biological tissue [2]. The method, providing high resolution and accuracy in mass and space, has been developed for a reliable identification and localization of individual tissue components, and has been applied recently to a number of research areas in pathology, cancer diagnostics, metabolic pathway analysis and plant research [2-5]. Targeted compound classes included phospholipids, peptides, proteins, drug compounds and metabolites. An atmospheric pressure (AP) ion source has been developed for these studies, based on a dedicated microoptical setup that provides highest spatial resolution of 3 to 5 micrometer on tissue in imaging mode on Orbitrap-based instrumentation.The advantages of Orbitrap detection are given here:1. High mass resolution allows to analyze complex tissue samples, sample complexity2. High mass accuracy enables for straightforward sum formula assignments and facilitates
substance identification, substance identificationAccurate MS and MS/MS imaging data allow to align images, confirmation
3. Irrelevance of sample morphology/flatness, ease of sample / matrix preparation4. Convenience of AP interface, compatibility to ambient analysis
High Resolution in Mass and Space: AP-MALDI Imaging using Orbitrap-based InstrumentationBernhard Spengler1,3, Andreas Römpp1, Sabine Günther1, Oliver Schulz1, Klaus-Peter Hinz1, Alfons Hester1, Christian Schinz1, Christian Lotze1, Jörg-Ulrich Pötzl1, Kerstin Strupat2
1Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Germany *[email protected] Fisher Scientific (Bremen) GmbH, Germany, [email protected], 3TransMIT GmbH, Giessen, Germany, [email protected]
Open-cover view of the atmospheric pressure
imaging ion sourceimagine10
(TransMIT GmbH, Giessen) attached to
a Thermo Scientific Q ExactiveTM
Orbitrap instrument, Thermo Fisher
Scientific (Bremen) GmbH
Scheme of the atmospheric pressure
imaging ion sourceimagine10
(TransMIT GmbH, Giessen) attached to a
Thermo Scientific Q ExactiveTM Orbitrap
instrument (Thermo Fisher Scientific (Bremen) GmbH) Coronal mouse brain section
35 µm steps, 295 x 195 pixels
Left: Focus size measurements (most left) and ablation on
tissue reveal an effective pixel
resolution in the range of 5 µm, effective pixel size if laser irradiance
dependent.
Above: Un-blurred red/green pixels indicate
an effective spatial resolution on mouse brain tissue (lateral ventricle)
of 3 µm.
Left:Single pixel (3 µm) = single scan
Exactive MS Orbitrap mass spectrum
Lateral ventricle, 3 µm steps, 170 x 200 pixels
Experimental
RMS = 0.2 ppm
+/-1
ppm
30,000 consecutive scans of PC(34:1) at m/z = 798.5410 on tissue during
image acquisiton using internal lock mass operation.
Representative single pixel (10 x 10 µm2) mass spectrum upon automated internal
lock mass operation. imagine10 source attached to Q Exactive
Orbitrap instrument
MS and MS/MS Imaging
A coaxial ion source geometry was employed to irradiate, desorb, ionize and transport biological compounds under atmospheric pressure conditions. Ions are introduced into the mass spectrometer through an
extended heated capillary. Transfer efficiency was optimized using both, gas stream dynamics and electrostatic ion focusing. The
achievable mass accuracy during tissue imaging operation is typically better than 1 ppm RMS using lock mass calibration. Imaging speed is
up to 2 pixels per second, depending on mass range and mass resolution setting. Matrix preparation and on-tissue protein digestion
were performed using a new dedicated protocol.
50 µm100 µm
Red: backgroundGreen: PC(38:4)Blue: PC(34:1)
Healthy mouse brain tissue. Two phospholipids differing by 8 mmu (m/z
848.55 and m/z 848.63) are extracted into an MSimage.
10 µm, 400 x 400 pixels (= 4*4 mm2) Mass Resolution = 70000 @ m/z 400
imagine10 source attached to Q Exactive Orbitrap instrumentation
T007 CK75 MS-MSMS772 200x200 10um E17 #37003 RT: 805.25 AV: 1 NL: 9.03E4T: FTMS + p NSI Full ms [700.00-900.00]
700 720 740 760 780 800 820 840 860 880 900m/z
0
10
20
30
40
50
60
70
80
90
100
Rel
ativ
e A
bund
ance
798.55066
772.53479
896.61188
782.57678826.58234 870.59595
716.13373 760.59467734.57874 848.56702
820.53528
876.59943
S194 CK3 170x200 3um E170_RC798#26687 RT: 548.39 AV: 1 NL: 6.75E3
FTMS + p NSI Full ms [100.00-1000.00]
700 720 740 760 780 800 820 840 860 880 900 m/z0
50
100
Rel
ativ
e Ab
unda
nce
798.5412
772.5258
896.6012
769.5638 782.5681 870.5860739.4668 848.5556713.4514 826.5722756.5528844.5272804.5507
893.4771
Mass Resolution on Tissue
Mass Selectivity & Dynamic Range
Closely neighbored
signals, even of drastically
differing peak intensities, can be
clearly distiguished and
discriminated. Bin width Δm/z was
0.01 u.
798.5416PC(34:1)+K+
798.5909PE(38:0)+Na+
797 798 799 800 801 802
798.5416
799.5453
800.5596801.5636
798.45 798.50 798.55 798.60m/z
0.0
0.2
0.4
0.6
0.8
1.0
1.2
Rel
ativ
e A
bund
ance
798.5990
798.4895
12.5 ppm window (Δm/z=0.01)
m/z
Healthy mouse brain tissue, 10 um step size, 200x200 pixels (= 2 *2 mm2). MS and MS/MS scans on adjacent, neigboring pixels.Left: m/z 772 (MS Orbitrap, full scan) right: m/z 713 (MSMS of 772), loss
of trimethylamine (59 u).MS/MS experiment confirms the phospholipid classification as phosphatidylcholine or shingomyeline. imagine10 source attached to Q Exactive Orbitrap instrument.
m/z 772.523 m/z 713.450
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