Guidance of neuronal growth with DEP
-
Upload
carolos-alex -
Category
Documents
-
view
26 -
download
0
description
Transcript of Guidance of neuronal growth with DEP
![Page 1: Guidance of neuronal growth with DEP](https://reader031.fdocuments.in/reader031/viewer/2022032605/56812c41550346895d90c6d3/html5/thumbnails/1.jpg)
Guidance of neuronal growth with DEP
J. James Jun
PresenterUniversity of Calgary
Dr. Rudolf PotucekSupervisorUniversity of Calgary
January 25, 2007
![Page 2: Guidance of neuronal growth with DEP](https://reader031.fdocuments.in/reader031/viewer/2022032605/56812c41550346895d90c6d3/html5/thumbnails/2.jpg)
2
Application of dielectrophoresis(DEP) for directing neuronal growth
Neuronal Guidance
Theory of DEP
Modeling & Experiment
![Page 3: Guidance of neuronal growth with DEP](https://reader031.fdocuments.in/reader031/viewer/2022032605/56812c41550346895d90c6d3/html5/thumbnails/3.jpg)
3
Neuronal growth: random vs. directed
Random Directed
![Page 4: Guidance of neuronal growth with DEP](https://reader031.fdocuments.in/reader031/viewer/2022032605/56812c41550346895d90c6d3/html5/thumbnails/4.jpg)
4
Electric forces: Electrophoresis vs Dielectrophoresis
Electrophoresis
Any electric fields
Charged object
DC
Dielectrophoresis
Spatially varying field
Polarizable object
DC or AC
EPF qE
DEPF p E
![Page 5: Guidance of neuronal growth with DEP](https://reader031.fdocuments.in/reader031/viewer/2022032605/56812c41550346895d90c6d3/html5/thumbnails/5.jpg)
5
Modeling and Experiment
Microsphere
BrainPond snail Neuron
Field Modeling Vision Tracking
![Page 6: Guidance of neuronal growth with DEP](https://reader031.fdocuments.in/reader031/viewer/2022032605/56812c41550346895d90c6d3/html5/thumbnails/6.jpg)
6
Experiment with polymer bead
![Page 7: Guidance of neuronal growth with DEP](https://reader031.fdocuments.in/reader031/viewer/2022032605/56812c41550346895d90c6d3/html5/thumbnails/7.jpg)
7
Electric field modelingQuadruple electrodes configuration
top
side
![Page 8: Guidance of neuronal growth with DEP](https://reader031.fdocuments.in/reader031/viewer/2022032605/56812c41550346895d90c6d3/html5/thumbnails/8.jpg)
8
Computer vision tracking
![Page 9: Guidance of neuronal growth with DEP](https://reader031.fdocuments.in/reader031/viewer/2022032605/56812c41550346895d90c6d3/html5/thumbnails/9.jpg)
9
Experiment with a live snail neuronChallenges
More complicated than the experiment with beads
Long term experiment (3 days)
Need an evaporation control with perfusion system
Need a neurotrophic factor enriched medium
![Page 10: Guidance of neuronal growth with DEP](https://reader031.fdocuments.in/reader031/viewer/2022032605/56812c41550346895d90c6d3/html5/thumbnails/10.jpg)
10
Conclusions DEP can be used to direct a neuronal growth with electric field modeling and computer vision trackingDEP is a non-contact force and bio-chemically safe
Electric field modeling to optimizes DEP parameters
Computer vision tracking to track a growthcone
Experiments planned with beads then snail neurons
Questions?