Grup de Transductors Químics - imb-cnm.csic.es · Lab on a chip fotònics i electroquímics Grup...

Lab on a chip fotònics i electroquímics

Grup de Transductors Químics gtq.imb-cnm.csic.es

Xavier Muñoz [email protected]

21 Maig, 2015

Institut de Microelectrònica de Barcelona (IMB-CNM, CSIC)

• Main Clean Room 1500 m2

Class 100-10000

• Basement Clean Room 40 m2

Class 100-1000

• Largest CR in South europe

http://gtq.imb-cnm.csic.es/ 21 Maig, 2015

Lab on a Chip A lab-on-a-chip (LoC) is a system that merges all the capabilities of a lab in a single millimiter-size system

http://gtq.imb-cnm.csic.es

Grup de Transductors Químics

Key Advantages • Small sample volume (μl) • Enhanced response time • Portable equipment • Low consumption


Nanofluidics

Nanophotonics

Nanoelectronics

Towards a Lab on a chip

Nanomedicine

http://gtq.imb-cnm.csic.es/

Elements included in a Lab on a chip

• Passive elements: Channels, reactors, mixers…

• Active elements Pumps, valves…

• Sensors Photonic, electrochemical….

at the micron-size level!!

microfluidics Inlets

Detection


What do we apply our Lab on chips for?

FOOD

• pH, ions • Ascorbic acid, lactate • Pathogens (i.e. Bacteria) •Wine: Ethanol, sugar, acidity, color, grape variety, vintage...

Matrices: Wine Milk Yoghurt Beverages Soup….

ENVIRONMENT

• pH, conductivity, Ions • Organic matter – chemical oxygen demand • Chlorine, turbidity • Heavy metals • Pathogens (i.e. Bacteria)

Scenarios: Lakes Purification plants Tap water Wastewater treatment plants


What do we apply our Lab on chips for?

BIOMEDICAL ANALYSIS

• Metabolites in biological liquids: pH, Na, Ca, glucose, urea,

• Illness indicators: Cancer, Reumatoid arthritis

• Drugs: Dopamine, paracetamol • Cell culture: Celular methabolism,

toxicity, cell counting

Escherichia coli

Vascular smooth

muscle cells (VSMC) Saccharomyces cerevisiae


Quantification approach Classification approach

Multiparametric system: Electronic Tongue

Analyze food products such as wines, beverages and soups


Electrical readout system Impedimetric analysis of bioarrays developed on glass slides

• Array 36 gold electrodes

• Applied to the measurement of proteins and haptens – different immunoassay formats.

• Analytical performance similar to fluorescent scanner approaches

Analysis of boldenone hormone

2 mm


Patterning of wax is used to fabricate microfluidic structures (reservoirs, pumps, valves)

Wax patterning down to 25 µm LOC with amperometric detection

Wax-based lab on a chip platform

Operation of a wax valve


PDMS n=1.41

Air n=1.00

Parallel beams

Beam concentrator

Microlenses Micromirrors

Flat air mirror Focusing air mirror

Hybrid Photonics

Polymer-based micro-optics/photonics elements


Polymeric absorbance microfilters

350 400 450 500 550 600 650 700-25

-20

-15

-10

-5

0

Tra

nsm

itta

nce

(d

B)

Wavelength (nm)

Quinoline yellow

Phenol red

Methyl orange

Crystal violet

Xerogel solid-state light emitters (SSLE)

Alexa 430

Rhodamine B

Non-doped

CdSe QDs

Hybrid Photonics Polymer-based micro-optics/photonics elements


14 http://gtq.imb-cnm.csic.es/

Photonic lab on a chip with integrated light emitters

• PhLoC including: Xerogel emitters, microlenses, micromirrors, channels for fiber optics alignment and functionalized enzymatic reactor

H2O2 detection via HRP

emitter

air mirror

air mirror

microlense

Interrogation region

Prepolymer solution outlet

Prepolymer solution intlet

Self-alingment channelInput fluidic port

Ouput fluidic port

LOD: 2.73 ± 0.05 μM

Sensitivity: 2.9 ± 0.2 A.U./mM.

21 Maig, 2015


Biophotonic Lab on a chip Simultaneous supernatant and cell culture screening

Fluidic outlet

Monitoring reactor

Cell seeding inlet Fluidic inlet

Retention reactor

Size-exclusion microfilters

Microlens

Air mirrors

Self-alignment channel

21 Maig, 2015

22/05/2015 17 http://gtq.imb-cnm.csic.es/

Integrated size-exclusion microfilters

Biophotonic Lab on a chip

21 Maig, 2015

22/05/2015 18 http://gtq.imb-cnm.csic.es/

Integrated size-exclusion microfilters

Biophotonic Lab on a chip

21 Maig, 2015

26 28 30 32 34 36 38

0.05

0.10

0.15

0.20

0.25

0.30

0.35

0.40

0.45

0.50

Rinsing

Retention reactor

Monitoring reactor

Ab

so

rba

nce

(A

.U.)

Time (min)

Trapping


• Suitable for cell culture purification, microorganism separation… while performing real time screening.

Size-dependent microorganism separation

Biophotonic Lab-on-chip

21 Maig, 2015


Acknowledgements

The research leading to these results has received funding from the

European Research Council under the European Community's

Seventh Framework Programme (FP7/2007-2013) / ERC grant agreement

n° 209243.

Project funded by the European Commission within the 7th

Framework Programme (FP7/2007-2013) Grant Agreement nº.

317916

21 Maig, 2015

http://erc.europa.eu/index.cfm

[email protected]

http://gtq.imb-cnm.csic.es/

Grup de Transductors Químics - imb-cnm.csic.es · Lab on a chip fotònics i electroquímics Grup...

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