Grapevine Shoot Tip Cultureiv.ucdavis.edu/files/24508.pdfrapevine Shoot Tip Culture 3 months Macro...
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Grapevine Shoot Tip Culture Sue Sim, Staff Research Associate Foundation Plant Services, UCD
Transcript of Grapevine Shoot Tip Cultureiv.ucdavis.edu/files/24508.pdfrapevine Shoot Tip Culture 3 months Macro...
Grapevine Shoot Tip Culture
Sue Sim, Staff Research AssociateFoundation Plant Services, UCD
Macroshoot tip culture
Eliminates Crown Gall Eliminates Crown Gall and Viruses
Microshoot tip culture
Grapevine Shoot Tip
Cut
10.0 mm
Macroshoot tip culture
Sterilize& stick
10.0 mm
3 months
Macroshoot tip culture
Sterilize& stick
10.0 mm
Microshoot tip culture
Sterilize
10.0 mm
SterilizeCut to < 0.5 mm
Microshoot tip culture
10.0 mm
SterilizeCut to < 0.5 mm
Microshoot tip culture
10.0 mm
0.4 mm
Sterilize
8‐12 months
Cut to < 0.5 mm
Microshoot tip culture
10.0 mm
0.4 mm
Microshoottip culture
Cut
Virus
Microshoottip culture
Cut
Shoot apex of Nicotiana benthamianainfected with flourescent‐labeled beet severe curly top virus visible in protophloem. Photo courtesy Li‐Fang Chen, UCDavis.
Macroshoot tip culture
Microshoot tip culture
•10.0 mm•Eliminates crown gall•50 plants/hour•80‐100% survive and develop roots•$50 / plant
•0.5 mm•Eliminates crown gall and viruses•10 plants/hour•10 – 80% survive and develop roots•$$$ / plant
Thank you
