Grain Size Analysis - ImageJ
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ANALYZE PARTICLESBY USING IMAGEJhttp://rsb.info.nih.gov/ij/docs/menus/analyze.html#ap
By Nguyen Hoang Viet, PhD.
Content
Where to download
How to run ImageJ
Image Processing & Calculation
Visit this link:http://rsbweb.nih.gov/ij/download.html
Note: please check your window first, 32- or 64-bit system
Where to download
First – Run ImageJ program and Load the your image file.
How to run ImageJ
Open Image File
Run ImageJ: Start/All Programs/ImageJ/
Open Image File: File/Open…, choose the file and click OK
Image Processing & Calculation
Step 1 – Convert to 8-bit & Crop
Open Image File: File/Open…, choose the file and click OK - Grain Size Calculation.jpg
Convert to 8-bit: Image/Type/8-bit.
Crop: Draw a rectangular shape then Image/Type/Crop.
Save the cropped file: File/Save as/Jpeg… -Grain Size Calculation-Crop.tif
Measurement scale unit
Draw a rectangular shape fits on scale bar. You will see its width to be 237 px.
So, 1 m equals 79px.
Step 2: Convert to grainy image
Image/Adjust/Threshold:
Other processing:- Process/Binary/Erode- Process/Binary/Dilate
Step 3: Analyze Particles
Analyze/Analyze Particles …
There are two data files:
Results.xls
Summary.xls
Average grain size:
708 px2= 0,113 m2.
How to set new scale bar
Analyze/Set Scale
Then, the unit in imageJwill be µm (micrometer)
Step 4: Analyze data
Based on two data files in Excel format, you should find a software for doing further analysis. Such software like: Excel, MatLab, Origin, SciLab*, SciDAVis* …
*: free software
Step 4: Analyze data (2)
On the ‚Results‛ dialog. Choose Results/Distribution… the set bin number for ‘specify bins’ field: 10, 50 and 100.
Based on Histogram dialogClick on List to get data list,then you should use other software to draw the graph.
Histograms of the grain sizes
Grain sizes of cropped images
Acknowledgement
Thank my students, ATP k52 (Fall 2010), for doing well this homework and have good suggestion for further processing.