GANPAT UNIVERSITY · Industrial Microbiology. 1st edition. Wiley Eastern Limited. 2 Crueger W and...

GANPAT UNIVERSITY

FACULTY OF SCIENCE

Teaching and Examination scheme

&

Syllabus of

B.Sc. Biotechnology

Semester V

Effective from July 2020

GANPAT UNIVERSITY FACULTY OF SCIENCE

REVISION OF TECHING & EXAMINATION SCHEME AND SYLLABUS Programme Bachelor of Science Branch/Spec. Biotechnology

Semester V Academic Council Approved Syllabus (in which the revision is carried out)

Notification No

Date

Effective from Academic Year 2020-21 Effective for the batch Admitted in July 2018

Subject code Subject Name Revision in Full Syllabus (Yes/No)

Revision in Teaching Scheme(Yes/No)

Revision in Exam Scheme(Yes/No)

Revision in Content (Yes/No)

Percentage of changes if content revision

BMBT5MOB Molecular Biology

Yes Yes Yes Yes 100 %

BBIT5BPT Bioprocess Technology


BBIT5EBT Environmental Biotechnology


BBIT5MDM Medical Microbiology

New Course -- -- -- --

BELE5BNF Bioinformatics Yes Yes Yes Yes 100 %

BELE5BFS Basics Of Forensic Science


BEL5EPD Entrepreneurship Development


MOOCs courses from SWAYAM PORTAL


NEED OF REVISION:

New Syllabus implemented as per UGC guidelines.

*Any one subject can be offered from the following list of elective subjects Elective

Sr. No. Subject Code Subject Name

1 BELE5BNF Bioinformatics

2 BELE5BFS Basics Of Forensic Science

GANPAT UNIVERSITY

FACULTY OF SCIENCE

TEACHING AND EXAMINATION SCHEME

Programme Bachelor of Science Branch/Spec. Biotechnology

Semester V


Sr. No.

Subject Code Subject Name

Teaching scheme Examination scheme (Marks)

Credit Hours (per week) Theory Practical

Lecture(DT) Practical(Lab.) Lecture(DT) Practical(Lab.) CE SEE Total CE SEE Total

L TU Total P TW Total

L TU

Total

P TW Total

1 BMBT5MOB Molecular Biology 3 -- 3 2 2 3 -- 3 3 1 4 40 60 100 40 60 100

2 BBIT5BPT Bioprocess Technology

3 -- 3 2 2 3 -- 3 3 1 4 40 60 100 40 60 100

3 BBIT5EBT Environmental Biotechnology

3 -- 3 2 2 3 -- 3 3 1 4 40 60 100 40 60 100

4 BBIT5MDM Medical Microbiology

3 -- 3 2 2 3 -- 3 3 1 4 40 60 100 40 60 100

5 Elective* 2 -- 2 - - 2 -- 2 - -- -- 40 60 100 -- -- --

Total 14 -- 14 08 08 14 -- 14 12 04 16 200 300 500 160 240 400

3 BEL5EPD Entrepreneurship Development

4 MOOCs courses from SWAYAM PORTAL

GANPAT UNIVERSITY

FACULTY OF SCIENCE


Semester V Version 2.0.0.0

Effective from Academic Year 2020-2021 Effective for the batch Admitted in July-2018

Subject code BBIT5BPT Subject Name Bioprocess Technology


(Per week) Lecture(DT) Practical(Lab) Total CE SEE Total

L TU P TW

Credit 03 -- 2 05 Theory 40 60 100

Hours 03 -- 3 1 07 Practical 40 60 100

Learning Outcome:

Students will understand the concept of fermentation and how to screen industrially important organisms.

Graduates will get to know the aspects of media and their formulation procedures with studying the principle and importance of each media ingredient.

Graduates will understand the different ways of sterilizing the industrially important equipment and media.

Graduates will understand the different types of fermenters, their construction,and mode of operation, monitoring and control.

Graduates will get to know the different industrially and commercially important fermentation products, pharmaceutical products and metabolites.

This will help in implementing the general principles and processes necessary for clear and better environment.

Theory syllabus

Unit Content Hrs

1 - Introduction to bioprocess technology. Range of bioprocess technology and its chronological development, Basic principle of fermentation technology. Microbial Strain Improvement techniques and Preservation.

- Types of microbial culture and its growth kinetics– Batch, Fedbatch and Continuous culture.

15

2 - Design of bioprocess vessels: Significance of Impeller, Baffles, Sparger; Types of culture/production vessels- Airlift; Cyclone Column; Packed Tower and their application in production processes.

10

3 - Principles of upstream processing: Media preparation & Sterilization, Inocula development.

- Introduction to oxygen requirement in bioprocess; mass transfer coefficient; factors affecting KLa.

- Bioprocess measurement and control system with special reference to computer aided process control.

10

4 - Introduction to downstream processing, product recovery and purification techniques.

10

- Microbial production of ethanol, amylase, citric acid and Single Cell Proteins.

Reference Books

1 Casida LE. (1991). Industrial Microbiology. 1st edition. Wiley Eastern Limited.

2 Crueger W and Crueger A. (2000). Biotechnology: A textbook of Industrial Microbiology. 2nd edition. Panima Publishing Co. New Delhi.

3 Patel AH. (1996). Industrial Microbiology. 1st edition, Macmillan India Limited.

4 Stanbury PF, Whitaker A and Hall SJ. (2006). Principles of Fermentation Technology. 2nd edition, Elsevier Science Ltd.

List of Practical

1 Bacterial growth curve.

2 Calculation of thermal death point (TDP) of a microbial sample

3 Production and analysis of Ethanol.

4 Production and analysis of Amylase.

5 Production and analysis of Citric acid.

6 Isolation of antibiotic producing organism by crowded plate method.

7 Isolation of amylase producing microorganism.

8 Enzyme purification by using ammonium sulphate precipitation.

9 Aeration Efficiency determination of Kla.

10 Isolation of antibiotic producing organism by Willkins method.

GANPAT UNIVERSITY

FACULTY OF SCIENCE




Subject code BBIT5EBT Subject Name Environmental Biotechnology



L TU P TW

Credit 03 -- 2 05 Theory 40 60 100

Hours 03 -- 3 1 07 Practical 40 60 100

Learning Outcome:

Students will learn the different pollutant present in environment and the various ways of transport of pollutants.

Graduates will understand the hazardousness of recalcitrant compounds and how biodegradation can be used for xenobiotic compounds.

Graduates will understand the different biochemical pathways for the degradation of petroleum and its derivatives.

Graduates will understand the importance of water and its physical, chemical and biological properties.

Graduates will get to know the different techniques of waste water treatments using primary, secondary and tertiary waste.

Graduates will understand transformations of pesticides, different microbiological aspects of waste management. They will know to preserve and protect the environment using eco-friendly methods.

Theory syllabus

Unit Content Hrs

1 - Conventional fuels and their environmental impact – Firewood, Plant, Animal, Water,Coal and Gas.

- Modern fuels and their environmental impact – Methanogenic bacteria, Biogas, Microbial hydrogen Production, Conversion of sugar to alcohol, Gasohol

10

2 - Bioremediation of soil & water contaminated with oil spills, heavy metals and detergents.

- Degradation of lignin and cellulose using microbes, Phyto-remediation. - Degradation of pesticides and other toxic chemicals by micro-organisms-

degradation aromatic and chlorinates hydrocarbons and petroleum products.

15

3 - Treatment of municipal waste and Industrial effluents. - Bio-fertilizers-Role of symbiotic and asymbiotic nitrogen fixing bacteria in the

enrichment of soil, Algal and fungal biofertilizers (VAM)

10

4 - Bioleaching-Types and Mechanisms, Enrichment of ores by microorganisms (Gold, Copper and Uranium).

10

- Environmental significance of genetically modified microbes, plants and animals.

Reference Books

1 Environmental Science, S.C. Santra

2 Environmental Biotechnology, Pradipta Kumar Mohapatra

3 Environmental Biotechnology – Concepts and Applications, Hans-Joachim Jordening and Jesef Winter

4 Waste Water Engineering, Metcalf and Eddy, Tata McGraw hill

5 Agricultural Biotechnology, S.S. Purohit

6 Environmental Microbiology : Methods and Protocols, Alicia L. Ragout De Spencer, John F.T. Spencer

7 Introduction to Environmental Biotechnology, Milton Wainwright

8 Principles of Environmental Engineering, Gilbert Masters

9 Wastewater Engineering – Metcalf & Eddy

List of Practical

1 Estimation of Total Dissolved Solids (TDS) of water sample.

2 Estimation of BOD of water sample.

3 Estimation of COD of water sample.

4 Bacterial Examination of Water by MPN Method.

5 Estimation of Total Solids (TS) of water sample.

6 Estimation of TSS of water sample.

7 Isolation of symbiotic Nitrogen fixing organisms.

8 Isolation of Asymbiotic Nitrogen fixing organisms.

9 Isolation of Xenobiotic degrading organism (Pesticide).

10 Isolation of Hydrocarbon degrading organisms.

GANPAT UNIVERSITY

FACULTY OF SCIENCE




Subject code BBIT5MDM Subject Name Medical Microbiology



L TU P TW

Credit 03 -- 2 05 Theory 40 60 100

Hours 03 -- 3 1 07 Practical 40 60 100

Learning Outcome:

Students will understand normal flora of skin, oral cavity and gastrointestinal tracts and other body regions.

Graduates will get to know entry and colonization of microorganisms and toxigenicity of microorganisms.

Graduates can analyses the different types of pathogens and pathogenic behavior in diseases causal.

Graduates will understand the various prophylaxis measures and the concepts of immunization.

Graduates will be trained in the concepts of different types of vaccines and their sources, advantages and disadvantages.

This will help in clearing the concepts of spread of infectious diseases, their causes,types, transmission and responses.

Theory syllabus

Unit Content Hrs

1 - Introduction: Normal microflora of human body, nosocomial infections, carriers, septic shock, septicemia, pathogenicity, virulence factors, toxins, biosafety levels. Morphology, pathogenesis, symptoms, laboratory diagnosis.

- Preventive measures and chemotherapy of gram positive bacteria: S.aureus, S.pyogenes, B.anthracis, C.perferinges,C.tetani, C.botulinum, C.diphtheriae M.tuberculosis, M. leprae.

- Basic of Bioethics and biosafety guideline related to Contamination, decontamination, disposal and safety from infectious sources.

15

2 - Morphology, pathogenesis, symptoms, laboratory diagnosis, preventive measures and chemotherapy caused by gram negative bacteria: E.coli, N. gonorrhoea, N. meningitidis, P.aeruginosa, S. typhi, S. dysenteriae, Y. pestis, B. abortus, H. influenzae, V. cholerae, M.pneumoniae, T. pallidum, M. pneumoniae, Rickettsiaceae, Chlamydiae.

10

3 - Diseases caused by viruses: Picornavirus, Orthomyxoviruses, Paramyxoviruses, Rhabdoviruses, Reoviruses, Pox virus, Herpes virus, Papova virus, Retro viruses (including HIV/AIDS) and Hepatitis viruses.

10

4 - Fungal and Protozoan infections:Dermatophytoses (Trichophyton, Microsporun andEpidermophyton) Subcutaneous infection (Sporothrix, Cryptococcus), systemic infection(Histoplasma, Coccidoides) and opportunistic fungal infections (Candidiasis, Aspergillosis), Gastrointestinal infections (Amoebiasis, Giardiasis), Blood-borne infections (Leishmaniasis, Malaria)

10

Reference Books

1 Brooks GF, Carroll KC, Butel JS and Morse SA. (2007). Jawetz, Melnick and Adelberg’s Medical Microbiology. 24th edition. McGraw Hill Publication.

2 Goering R, Dockrell H, Zuckerman M and Wakelin D. (2007). Mims’ Medical Microbiology. 4th edition. Elsevier.

3 Willey JM, Sherwood LM, and Woolverton CJ. (2008). Prescott, Harley and Klein’s Microbiology. 7th edition. McGraw Hill Higher Education

List of Practicals

1 Identification of pathogenic bacteria based on culturaland morphological characterization.

2 Identification of pathogenic bacteria based on biochemical characteristics.

3 To perform antibacterial testing by Kirby-Bauer method

4 To prepare temporary mounts of Aspergillus and Candida by appropriate staining.

5 Identification of pathogen using Gram’s staining method.

6 Identification of pathogen using Acid fast staining.

7 Identification of pathogen using Capsule staining.

8 Identification of pathogen using spore staining.

9 Study of skin flora.

10 To prepare temporary mounts of Candida by appropriate staining.

GANPAT UNIVERSITY

FACULTY OF SCIENCE

Programme Bachelor of Science Branch/Spec. Biotechnology/Microbiology



Subject code BELE5BNF Subject Name Bioinformatics


(Per week) Lecture(DT)

Practical(Lab.)

Total

CE SEE Total

L TU P TW

Credit 02 -- -- -- 02 Theory 40 60 100

Hours 02 -- -- -- 02 Practical -- -- --

Learning Outcome:

Students will understand introduction and importance of bioinformatics.

Graduates will get to know applications of bioinformatics.

This can be further used for studying biological databases.

Graduates will understand human genome project.

This course will help in understand biological sequence analysis.

Graduates will get knowledge about sequence similarity tools.

Theory syllabus

Unit Content Hrs

1 - BIOINFORMATICS AND BIOWEB RESOURCES: Introduction: History, scope and importance of bioinformatics, The notion of Homology.

- Bioinformatics Applications: Phylogenetic analysis, Pharmacogenomics (Drug Discovery), Crop Genomics (Agroinfomatics), Metabolomics, In-silico drug designing, Docking, Systems biology, Genome annotation.

- Searching the biological search Engines (Entrez, SRS, Scirus), Biological databases (Nucleotide, Protein), Data submission.

15

2 - BIOLOGICAL DATA GENERATION AND SEQUENCE ANALYSIS: Human Genome Project: Objective, History, major landmarks, Method of Sequencing Human Genome, Status of HGP, Application and ELSI.

- Sequence alignment: Local, Global, Pairwise & Multiple sequence alignment, Sequence similarity tools (BLAST, FASTA), Detecting Open Reading Frames, Hierarchy of protein structure - primary, secondary, tertiary and quaternary structures, Ramachandran plot.

15

Text Books

1 Westhead, D. R. and Twyman, R. M. Instant Notes: Bioinformatics. Viva Publication.

2 S. C. Rastogi, Parag Rastogi, Namita Mendiratta, Bioinformatics Methods And Applications: Genomics Proteomics And Drug Discovery.

3 Arthur M Lesk. Introduction to Bioinformatics. Oxford University Press.

4 Zhumar Ghosh and Bibekanand Mallick. Bioinformatics: Principles and Applications. Oxford University Press.

5 Attwood T K, Parry D J, Phukan Samiron. Introduction to Bioinformatics. Pearson Education.

6 Cyntbia Gibas, Jambeck, Per, O’Reilly. Developing Bioinformatics Computer Skills.

Reference Books

1 David Mount, Bioinformatics: sequence and genome analysis, Cold Springer Harbour press.

2 Pevsner J. (2009) Bioinformatics and Functional Genomics. II Edition. Wiley-Blackwell

3 Oren, Jones and Thorntor, Bioinformatics, Gene Proteins and Computers, Advance text, Bios publication.

4 Primrose and Twyman (2003) Principles of Genome Analysis & Genomics. Blackwell

5 Campbell A. M., Heyer L. J. (2006) Discovering Genomics, Proteomics and Bioinformatics. II Edition. Benjamin Cummings.

6 A. Baxevanis and B.F. Ouellette, Bioinformatics: A practical Guide to the Analysis of Genes and Proteins, Wiley- Interscience, Hoboken, NJ 1998

GANPAT UNIVERSITY

FACULTY OF SCIENCE




Subject code BELE5BFS Subject Name Basics of Forensic Science



L TU P TW

Credit 02 -- - 02 Theory 40 60 100

Hours 02 -- - - 02 Practical 40 60 100

Learning Outcome:

Students will understand principles of forensic science.

Graduates will get to know forensic science laboratory, its organization and service, tools and techniques in forensic science.

Graduates will learn role of toxicologist, significance of toxicological findings.

Graduates will understand classification of fingerprints.

This course will help in learning principles of DNA fingerprinting, application of DNA profiling in forensic medicine.

This will help to learn investigation tools,search and seizures of computers and cyber security.

Theory syllabus

Unit Content Hrs

1 - Introduction and principles of forensic science, forensic science laboratory and its organization and service, tools and techniques in forensic science, branches of forensic science, causes of crime, role of modus operandi in criminal investigation.

- Classification of injuries and their medico-legal aspects, method of assessing various types of deaths.

15

2 - Principle of DNA fingerprinting, application of DNA profiling in forensic medicine, Investigation Tools, eDiscovery, Evidence Preservation, Search and Seizure of Computers, Introduction to Cyber security.

15

Reference Books

1 Molecular Biotechnology- Principles and Applications of recombinant DNA. ASM Press, Washington.

2 B.B. Nanda and R.K. Tiwari, Forensic Science in India: A Vision for the Twenty First Century, Select Publishers, New Delhi (2001).

3 M.K. Bhasin and S. Nath, Role of Forensic Science in the New Millennium, University of Delhi, Delhi (2002).

4 S.H. James and J.J. Nordby, Forensic Science: An Introduction to Scientific and Investigative Techniques, 2nd Edition, CRC Press, Boca Raton (2005).

5 W.G. Eckert and R.K. Wright in Introduction to Forensic Sciences, 2nd Edition, W.G. Eckert (ED.), CRC Press, Boca Raton (1997).

6 R. Saferstein, Criminalistics, 8th Edition, Prentice Hall, New Jersey (2004).

7 W.J. Tilstone, M.L. Hastrup and C. Hald, Fisher’s Techniques of Crime Scene Investigation, CRC Press, Boca Raton (2013).

GANPAT UNIVERSITY

FACULTY OF SCIENCE




Subject code BEL5EPD Subject Name Entrepreneurship Development



L TU P TW

Credit 03 -- - 03 Theory 40 60 100

Hours 03 -- - - 03 Practical 40 60 100

Learning Outcome:

Graduates will understand meaning, need and importance of entrepreneurship.

Graduates will get to know forms of business organization, project identification, project formulation and assessment of feasibility.

Graduates will get to know forms of business organization, project identification, project formulation and assessment of feasibility.

Graduates will understand meaning and importance of marketing management, physical distribution and stock management.

This course will help in learning inventory direct and indirect raw materials and its management.

Graduates will get to know meaning of international business, selection of a market for international business and export financing.

Theory syllabus

Unit Content Hrs

1 - Meaning, Needs and Importance of Entrepreneurship, Understanding essentials of techno-entrepreneurship concept :Market, Branding

- Factors influencing entrepreneurship, Features of a successful Entrepreneurship. - Forms of Business Organization, Project Identification, Selection of the product,

Project formulation, Assessment of project feasibility.

10

2 - Importance of finance / loans and repayments, Characteristics of Business finance

- Fixed capital management: Sources of fixed capital, working capital its sources and how to move for loans, Inventory direct and indirect raw materials and its management.

20

Reference Books

1 Holt DH. Entrepreneurship: New Venture Creation.

2 Kaplan JM Patterns of Entrepreneurship

3 Gupta CB, Khanka SS. Entrepreneurship and Small Business Management, Sultan Chand & Sons.

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