From the Human Eye Down to Microscopes at the Nano-Scale
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Transcript of From the Human Eye Down to Microscopes at the Nano-Scale
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Notre Dame extended Research Community
From the Human Eye Down to Microscopes at the Nano-Scale
Michael CrockerValerie GossPat MooneyRebecca Quardokus
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Outline Discussion of Optics
History Ranges of Visual Resolution
Scanning Electron Microscopy (SEM) Fundamentals Operation Scheme
Atomic Force Microscopy (AFM) Scanning Tunneling Microscopy (STM)
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Scanning Bunny
A distant cousin of the energizer bunny who helps to explain nanoscience
concepts.WELCOME PENN HIGH SCHOOL
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target
sourceHow do we see an object?
detector…and often you’ll need a lens
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Requirements of Vision The light that reaches the eye must have a color
between red (760nm) and blue (400nm) – or a mixture of these colors
The light that reaches the eye must be sufficiently bright – usually requires a sufficiently bright source
visible light
wavelength in nm400 760 3000760290 320
www.uvabcs.com/uvlight-typical.php , August 31, 2009
infrared
UV UV UV
C B A
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Object and Source Matching
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Seeing Atomic Structure Light must be about 0.1nm in wavelength to see
atomic structure: x-rays But our eyes can’t detect x-rays - 0.1nm light -
(5000 times smaller wavelength than we can see) Options
• Use x-rays and detector (to replace the eye)• Use particles (e.g. electrons) and detector
Electrons of the appropriate wavelength are easier to produce and direct than light – Scanning Electron Microscope (SEM)
Alternate imaging techniques• Atomic Force Microscope (AFM)• Scanning Tunneling Microscope (STM)
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Scanning Electron MicroscopeMichael Crocker
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Basic Idea?Let’s bounce
something else at the surface!
Animal sight and traditional
microscopes collect deflected light
e-e-
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Some are absorbed
Some are “reflected”
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Electron Beam Column
http://bioweb.usu.edu/emlab/TEM-SEM%20Teaching/How%20SEM%20works.html
Beam created from heated filamentBeam travels through a vacuumElectro-magnetic fields act as
lenses
Scattered and “secondary” electrons are detected
Electron beam hits the sample in a precise
location
Beam scans back and forth
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Electrons Hit Surface and Detection
http://www4.nau.edu/microanalysis/Microprobe-SEM/Signals.html
Primary electrons come from the beamSome scatter back,
others dislodge electrons
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Example Images
http://gsc.nrcan.gc.ca/labs/ebeam/sem_gallery_e.php
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Atomic Force Microscope
Valerie Goss
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What is the AFM?We can sense with
our hands by touching. An analogue!
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AFM cantilever and AFM tips
www.veeco.com
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The powerful, versatile AFM
~30 um scan
www.nanotech-now.com/.../antonio-siber.htm Aug 27, 2009
Resolutions: X and Y 2 -10 nmZ 0.05 nm
Microstructure of solids: CD, glass beads, circuits
Biological samples: skin cross section, viruses,
bacteria, blood, DNA and RNA
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Scanning Tunneling Microscope
Rebecca Quardokus
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Scanning Tunneling Microscopy (STM)
Electrons tunnel!
With a higher probability than cars
STM measures the current created by tunneling electrons
Images courtesy ofhttp://www.ieap.uni-kiel.de and www.renault.com
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Scanning Tunneling Microscopy (STM)
Image courtesy ofhttp://nano.tm.agilent.com
C60 “Bucky Balls”
Each C60 diameter is ~ 10Å1 Å = 1x 10-10 m
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Scanning Tunneling Microscopy (STM)
Xenon on Nickel
Iron on CopperImages courtesy of http://www.almaden.ibm.com
Individual atoms? That’s small!
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I’ll show you more in the microscope laboratory!