Friday September 18th

In Notebook:What experience do you have

using a microscope?What are some practical uses of a microscope that you are aware of?

Today’s Learning Targets

Agenda:

1.Microscope Notes

2.Microscope LabDUE: Tuesday Sept. 22nd

What’s my power?To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective.

Eyepiece is 10x and objective is 10x what is total magnificaion?

Basic Rules and Tips

• Carry using arm and base• Flat Surface• Adjust light using diaphragm • Use stage clips• Focus with course adjustment on low and

medium power only!– High Power use fine adjustment only

How to make a wet-mount slide …

1 – Get a clean slide and coverslip from your teacher.

2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess!

3 – Place the edge of the cover slip on one side of the water drop.

4 - Slowly lower the cover slip on top of the drop.

Cover Slip Lower slowly

Monday September 21st

In Notebook:How do you calculate total

magnification of an object through a microscope?

Today’s Learning Targets

Agenda:

1.Microscope Notes

2.Microscope Measuring LabDUE: ?

3.Friday’s Microscope Lab due TODAY at end of hour

4.REMINDER: Lab report due TOMORROW! (Do not need to print)

5.REMINDER: Quiz TOMORROW and test on THURSDAY!

1. Clear ruler

2. Determine how many millimeters across the field.(1 millimeter is the distance

from the middle of one mark mark to the middle of

the next mark)

3. Convert to micrometers(1mm=1000μm)

Calculate Field of View

Examples:1. If it’s 1.5 mm across, then the field of view is:

2. If it’s 2.4 mm across, then the field of view is:

DOES THE FIELD OF VIEW SIZE CHANGE WITH DIFFERENT MAGNIFICATIONS?

• Low power = 4 mm or 4000 μm• Medium power = 1.5 mm or 1500 μm• High power = .5 mm or 500 μm

Calculate Size of Specimen1. Figure out how many will fit across the field of

view (estimate)2. Divide field of view length by how many fit

acrossExample: If 7 protozoa fit across my field of view with a length of 1400μm, then what is the size of one protozoa?

PracticeLooking through my low power objective I see 4 mm on the ruler. Later I was looking at my hair and I estimate I could fit 10 hairs side by side in my field of view. How wide is my hair?