FORGED UNDER THE SUNRasuk et al., 2017, Photochemistry and Photobiology. HAAL resistome. High UV...
Transcript of FORGED UNDER THE SUNRasuk et al., 2017, Photochemistry and Photobiology. HAAL resistome. High UV...
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FORGED UNDER THE SUNMICROBES AND PHOTORECEPTORS FROM EXTREME ANDEAN PUNA
Dra. Virginia H. AlbarracínInvestigadora Independiente CONICET
Responsable Científica CIME-UNT-CONICET
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Outline
HAALPoli-Extremophiles
biodiversity UV-metaresitome
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The HAAL
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L. Verde
Tolar Grande
Antofalla
L. SocompaAlbarracin et al., 2016, Photochemistry and Photobiology
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HAAL: a highly solar irradiatedenvironment
Mean daily total solar irradiance (in W/m2) and Local Noon Time UV Index in the period 2005-2011 for all the Earth (right image)
and on the Puna of Atacama region (left image). The highest mean value of all the Earth in the range of (308 ± 13) W/m2. Mean UV
index value of 13.5± 1.0. Adapted from NASA OMI/Aura Online Visualization and Analysis (Daily Level 3 Global Gridded Products)
http://gdata1.sci.gsfc.nasa.gov/.Albarracin et al., 2015, Frontiers in Microbiology
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HAAL
microbiodiversity
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Microbial communities
from Bacterioplankton
Microbial communities
from the Benthos/soil
Microbial communities fromFlamingo faeces
Microbial mat communities
EMAMs
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Albarracin et al., 2016, Photochemistry and Photobiology
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UV-RESISTANT ACTINOBACTERIA
Rasuk et al., 2017, Photochemistry and Photobiology
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HAAL resistome
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High UV irradiation(among other extremes)
High microbiodiversity(microecosystem, species and gene levels)
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HAAL UV-resistome
UV-Resistome
Sensing and response regulators
Efficient photoprotection
Pigments, Extremolytes or
other metabolites
Efficient stress response/energy
administration
High tolerance to damage
Efficient repairingsystems for DNA
damage?
PR
Photolyases
DR
NER and others
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Photoreceptors
Light
Sensing
Response
ResistanceMechanisms
Metabolites
Comunication
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Model ExtremophilesPhysiology/Multi-omics
Extremo-enzymesExtremolytes
MicroecosystemMetagenomes
UV-Resistome
Approaches
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“Model extremophiles”
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Lake Verde (4,400 m)
Catamarca
Maximum UV-B 10.8 W/m2
As: 0.8 ppm
P: 0.012 ppm
Salinity: 5%
Acinetobacter sp. Ver3
Model UV-resistant Acinetobacter sp. Ver3
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Ver3 UV-Resistome: Genomics
Kurth et al., 2015, Frontiers in Microbiology
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Ver3 UV-resistome: Proteomics
0
0.2
0.4
0.6
0.8
1
1.2
1.4
0 5 10 15 20
Spot N Proteomic change Control/UV
02-Pyridine nucleotide disulfide oxidoreductase
04- Diaminobutirate-2-oxoglutarate aminotransferase
14-Dihidroxy acid dehydratase
15- Acetyl CoA hydrolase
23- Histidyl RNAt synthetase
33- Electron transfer flavoprotein sub-beta
35- Catalase
37- Fatty acid oxidation complex sub-alpha
40- Hypotetical protein
163 differential spots
25 significantly different
ROS scavengingQuorum sensing regulatorAA and protein synthesis
Beta oxidation of fatty acidsGlycolisis
Electron transfer chain
Kurth et al., 2015, Frontiers in Microbiology
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Ver3 UV-resistome: Ultrastructuromics
Galvan et al., 2018, en preparación
Mag. 20.00 kx - Escala: 200 nm Mag. 50.00 kx - Escala: 100 nmMag. 10.00 kx - Escala: 500 nm
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Ver3 UV-resistome: Ultrastructuromics
Galvan et al., 2018, en preparaciónMag. 50.00 kx - Escala: 100 nmMag. 10.00 kx - Escala: 500 nmMag. 4.00 kx - Escala: 1 µm
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Ver3 UV-resistome: Ultrastructuromics
Mag. 10.00 kx - Escala: 500 nm Mag. 20.00 kx - Escala: 200 nmMag. 2.00 kx - Escala: 2µm
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Ver3 UV-resistome: Ultrastructuromics
Mag. 2.00 kx - Escala: 2 µm Mag. 10.00 kx - Escala: 500 nm Mag. 20.00 kx - Escala: 200 nm
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Dra. Virginia AlbarracínGroup Leader
Investigadora Independiente
The A-Team-Laboratorio de Microbiología Ultraestructural y Molecular
-Laboratorio de Microscopía Molecular
Lic
Lic
Lic
Lic
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Deutscher Akademischer Austauschdienst
Max-Planck Society
Marie Curie Actions (IIF), FP7, UE
Argentinean Chapter MCAAEuropean Research Council