FORGED UNDER THE SUNRasuk et al., 2017, Photochemistry and Photobiology. HAAL resistome. High UV...
Transcript of FORGED UNDER THE SUNRasuk et al., 2017, Photochemistry and Photobiology. HAAL resistome. High UV...
FORGED UNDER THE SUNMICROBES AND PHOTORECEPTORS FROM EXTREME ANDEAN PUNA
Dra. Virginia H. AlbarracínInvestigadora Independiente CONICET
Responsable Científica CIME-UNT-CONICET
Outline
HAALPoli-Extremophiles
biodiversity UV-metaresitome
The HAAL
L. Verde
Tolar Grande
Antofalla
L. SocompaAlbarracin et al., 2016, Photochemistry and Photobiology
HAAL: a highly solar irradiatedenvironment
Mean daily total solar irradiance (in W/m2) and Local Noon Time UV Index in the period 2005-2011 for all the Earth (right image)
and on the Puna of Atacama region (left image). The highest mean value of all the Earth in the range of (308 ± 13) W/m2. Mean UV
index value of 13.5± 1.0. Adapted from NASA OMI/Aura Online Visualization and Analysis (Daily Level 3 Global Gridded Products)
http://gdata1.sci.gsfc.nasa.gov/.Albarracin et al., 2015, Frontiers in Microbiology
HAAL
microbiodiversity
Microbial communities
from Bacterioplankton
Microbial communities
from the Benthos/soil
Microbial communities fromFlamingo faeces
Microbial mat communities
EMAMs
Albarracin et al., 2016, Photochemistry and Photobiology
UV-RESISTANT ACTINOBACTERIA
Rasuk et al., 2017, Photochemistry and Photobiology
HAAL resistome
High UV irradiation(among other extremes)
High microbiodiversity(microecosystem, species and gene levels)
HAAL UV-resistome
UV-Resistome
Sensing and response regulators
Efficient photoprotection
Pigments, Extremolytes or
other metabolites
Efficient stress response/energy
administration
High tolerance to damage
Efficient repairingsystems for DNA
damage?
PR
Photolyases
DR
NER and others
Photoreceptors
Light
Sensing
Response
ResistanceMechanisms
Metabolites
Comunication
Model ExtremophilesPhysiology/Multi-omics
Extremo-enzymesExtremolytes
MicroecosystemMetagenomes
UV-Resistome
Approaches
“Model extremophiles”
Lake Verde (4,400 m)
Catamarca
Maximum UV-B 10.8 W/m2
As: 0.8 ppm
P: 0.012 ppm
Salinity: 5%
Acinetobacter sp. Ver3
Model UV-resistant Acinetobacter sp. Ver3
Ver3 UV-Resistome: Genomics
Kurth et al., 2015, Frontiers in Microbiology
Ver3 UV-resistome: Proteomics
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0.8
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1.2
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Spot N Proteomic change Control/UV
02-Pyridine nucleotide disulfide oxidoreductase
04- Diaminobutirate-2-oxoglutarate aminotransferase
14-Dihidroxy acid dehydratase
15- Acetyl CoA hydrolase
23- Histidyl RNAt synthetase
33- Electron transfer flavoprotein sub-beta
35- Catalase
37- Fatty acid oxidation complex sub-alpha
40- Hypotetical protein
163 differential spots
25 significantly different
ROS scavengingQuorum sensing regulatorAA and protein synthesis
Beta oxidation of fatty acidsGlycolisis
Electron transfer chain
Kurth et al., 2015, Frontiers in Microbiology
Ver3 UV-resistome: Ultrastructuromics
Galvan et al., 2018, en preparación
Mag. 20.00 kx - Escala: 200 nm Mag. 50.00 kx - Escala: 100 nmMag. 10.00 kx - Escala: 500 nm
Ver3 UV-resistome: Ultrastructuromics
Galvan et al., 2018, en preparaciónMag. 50.00 kx - Escala: 100 nmMag. 10.00 kx - Escala: 500 nmMag. 4.00 kx - Escala: 1 µm
Ver3 UV-resistome: Ultrastructuromics
Mag. 10.00 kx - Escala: 500 nm Mag. 20.00 kx - Escala: 200 nmMag. 2.00 kx - Escala: 2µm
Ver3 UV-resistome: Ultrastructuromics
Mag. 2.00 kx - Escala: 2 µm Mag. 10.00 kx - Escala: 500 nm Mag. 20.00 kx - Escala: 200 nm
Dra. Virginia AlbarracínGroup Leader
Investigadora Independiente
The A-Team-Laboratorio de Microbiología Ultraestructural y Molecular
-Laboratorio de Microscopía Molecular
Lic
Lic
Lic
Lic
Deutscher Akademischer Austauschdienst
Max-Planck Society
Marie Curie Actions (IIF), FP7, UE
Argentinean Chapter MCAAEuropean Research Council