Forensic Toxicology
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Transcript of Forensic Toxicology
Forensic Toxicology
Toxicology
• Definition: Analysis of body fluids or tissues for the presence of 1) controlled substances, 2) common pharmaceuticals and 3) poisons.
• What do Toxicologists know?– All about:
• drug interactions, metabolism, therapeutic vs. toxic doses, and impairment
Challenges…
• Identify 1 in 1000 drugs/poisons needle in a haystack
• Find tiny (nanogram to microgram) quantities, through the entire body
• Not always looking for exact chemicals
…metabolites are breakdown products Examples: heroin morphine in seconds
THC THC-9 in urine
Forensic Toxicology
Father of Modern Toxicology…
Orfila
Paracelsus "All things are poison and nothing is without poison, only the dose permits something not to be poisonous.”
(1530 AD)
Toxicology…when do we do it ??Antemortem = before death• DUIs BREATH
• Drug Facilitated Sex.Asslt.
BREATH, URINE
• Workplace Drug Testing
BREATH, URINE
• Parole violations BREATH
Post mortem = after death
• Homicides • Suicides• Overdose • MVA • Unexplained
death• Natural
(sometimes)
Toxicology of Alcohol
• Alcohol is absorbed into the blood, via stomach and small intestine
• Once absorbed, alcohol is:– Oxidized (oxidation = burned, with O2) in
liver– Excreted in breath and urine…
AS UNCHANGED PURE ALCOHOL !!
Alcohol in the Circulatory System
• Quantity of alcohol in the blood is…
…how drunk you are.
• Two methods of making this measurement– Measure alcohol in blood – Measure alcohol in breath
Circulation Con’t
Ratio of blood alcohol to alveoli air is approximately 2100 to 1– Means 2100 mL of Breath has the
same alcohol as 1mL of Blood
The Breathalyzer
Breathalyzer Con’t• 3 types of breathalyzers
• Measure alcohol in different ways
– Chemical… alcohol some other chemical
– Fuel Cell… alcohol electricity
– Infrared (IR) alcohol reflects IR back
Infrared and Fuel Cell Breath Tests• Infrared Breath Test
uses infrared wavelengths to test for alcohol
• Fuel Cell Test converts fuel (alcohol) and oxygen into a measurable electric current
Field Sobriety Testing
• Two reasons for the field sobriety test:
1. Assess suspects’ physical impairment
2. Determine need of evidential (urine) test.
Field Sobriety Tests• Horizontal Gaze Nystagmus
– Involuntary eye jerk as eye moves horizontally
• Walk and Turn (divided attention tasks)• One-Leg Stand
Horizontal Gase Nystagmus - CLIPhttp://www.bing.com/videos/search?
q=horizontal+gaze+nystagmus+video&view=detail&mid=95C10FAF1ABA03518D1A95C10FAF1ABA03518D1A&first=0&qpvt=horizontal+gaze+nystagmus+video&adlt=strict
Alcohol and the Law• 1939-1964:
intoxicated = 0.15% BAC
• 1965: intoxicated = 0.10% BAC
• 2003: intoxicated = 0.08% BAC
At least we don’t live in France, Germany, Ireland, or Japan (0.05%) or especially Sweden (0.02%)!
Drug Testing in the Body• Urine is most common• Immunoassay
– Based on antigen-antibody binding specificity
– Antibody produced by injecting antigen (drug) into rabbit
– Antibodies will bind to drug in urine
• Confirmation done w/TLC
False positives in Immunoassays
• Poppy seeds and Vicks 44 for opiates
• Decongestants for amphetamines
• Benadryl and numerous cough syrups for PCP