Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen...

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Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry Yale Forensic Genetics Unit, University of Santiago de Compostela, Spain Chris Phillips

Transcript of Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen...

Page 1: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry

Yale!"#$%$#&!'

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Forensic Genetics Unit, University of Santiago de Compostela, Spain

Chris Phillips

Page 2: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

• 140-SNP Identification panel

Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence loci

• Unforeseen complications with alignment when porting SNPs to MPS

• Considering linkage as forensic marker sets expand

• The ICMP ‘identification in cases of missing persons’ panel

• Genomic assessments of multiple-allele SNP-based polymorphisms

• Tailoring microhaplotype data compilation to forensic needs

• The EUROFORGEN Global AIMs+ and ‘NAME’+ ancestry panels

• Obtaining SNP data for regions with poor sampling - checkerboards

• Adapting ancestry analysis to multiple autosomal marker types

• USC assessed the panel with Ion PGM and did not use QIAseq then

Page 3: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

• Evaluation of the markers

Evaluation of new forensic DNA tests in the MPS era

• Are forensic loci detected and genotyped by MPS as expected ?

• Evaluation of the test’s performance

• Genotype patterns of new tri-allelic SNPs or MH loci - sequence data

• Evaluation and re-development of data analysis regimes

• Setting the extent of polymorphism in each MH; automated phasing

• Obtaining reference data for relevant populations - expensive

• Experimental validation: dilution series / mixtures / bones / controls

• Genotype patterns of binary SNPs - universal controls (used globally)

• Adapting ancestry analysis for inclusion of multiple-allele data

New

MASS loci

Evolving QIAseq

methods

Adapting data analysis/population data for M

Hs

Page 4: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

The Qiagen 140-SNP identification panel

Page 5: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

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rs1478829.rs1358856"

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rs4288409.rs2056277"

Kosambi(adjusted.Rc.value

.

679.nt.

55,162.nt.

36,472.nt.

349,542.nt.

Combining two independent panels into one set can lead to very close linkage - but this allows us to anticipate 1000+ SNP panels

D6S1042-SE33 Rc of 4%

Kiddlab pairs

SNPforID pair

x SNPforID-Kiddlab

Page 6: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Alignment challenges can be anticipated: polymeric tracts, or unanticipated: untracked flanking deletions (often population-specific)

Page 7: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

The ICMP ‘identification in cases of missing persons’ panel

ICMP2

The first forensic panel with no binary SNPs

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PentaE" D12S391" PentaD" FGA" D21S11" D22S1045" D6S1043" D18S51" D19S433" D1S1656" D2S441" D3S1358" D13S317" D7S820" D16S539" D10S1248" D2S1338" D20S482"" D17S1301" D5S818" D9S1122"" TH01" TPOX" vWA" D8S1179" CSF1PO" D4S2408""0"

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Pent

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D12S

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Pent

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FGA

D21S

11

D22S

1045

D6S1

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D18S

51

D19S

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D1S1

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D2S4

41

D3S1

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D13S

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D7S8

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TH01

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VWA

D8S1

179

CSF

1PO

D4S2

408

≥200

bp

143-173 bp <126 bp

Forensic MPS tests aim to sequence the shortest possible DNA fragments, but SNPs usually have two alleles - so lack power of STRs

Page 9: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

FP1!FP2!

• 1457 markers were incorporated after linkage screening - only 6 sites were eliminated based on primer extension disqualification

• 1377 autosomal tri-alleleic SNPs

• 34 tri-allelic X chromosome SNPs

• 46 microhaplotypes with 2, 3, 4, and 5 SNP combinations

• 2832 target enrichment extension primers - 80% of sites with redundant targeting

• 1195 markers currently under further evaluation

• 1123 autosomal tri-alleleic SNPs

• 28 tri-allelic X chromosome SNPs

• 44 microhaplotypes with 2, 3, 4, and 5 SNP combinations

Tri-allelic SNP and microhaplotype components

Page 10: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Tri-allelic SNPs with less frequent third alleles have very similar power to loci with three common alleles - the number of alleles is the important factor

Page 11: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

STR sequence variants and MicrohaplotypesHaplotype spans (between the bounding SNPs) are often longer than 200 nucleotides - so need to balance length with power

Page 12: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

S ASN

EUR

AFR

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4 12 20 22 43 33 2 24 40 28 34 26 13 36 31 46 39 32 7 45 35 10 14

Kiddlab

USCKiddlab microhaplotype sizes

ICMP panel microhaplotype sizes

46 Microhaplotypes ranked by descending size - as originally described in Kiddlab list of 130

Average size 128-NT

Average size 60.5-NT

16 microhaplotypes had identical sizes, 14 of these were at the extreme size range with an average 55-nucleotide size

65% of microhaplotypes adopted for the ICMP panel had their haplotype spans reduced by an average of 67 nucleotides

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Page 13: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

MH-21 73 nt

KHV JPT CHS CHB CDXTSI IBS GBR FIN CEU STU PJL ITU GIH BEB

YRI MSL LWK GWD ESNPEL

GGCTGGCC

CGTTCGTCCGCTCGCCCACT

AGTTAGTCAGCT

GGTC

GGTT

AGCC

Many microhaplotypes satisfy the need for short fragments and maximum levels of polymorphism from the sequenced strand

Page 14: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

MH-3 39 nt

TCC

ATT

ATC

ACT

ACC

KHV JPT CHS CHB CDXTSI IBS GBR FIN CEU STU PJL ITU GIH BEB

YRI MSL LWK GWD ESNPEL

TTC

TCT

TTT

Many microhaplotypes satisfy the need for short fragments and maximum levels of polymorphism from the sequenced strand

Page 15: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

MH-9 51 nt

GAT

GAC

AGC

AAC

KHV JPT CHS CHB CDXTSI IBS GBR FIN CEU STU PJL ITU GIH BEB

YRI MSL LWK GWD ESNPEL

GGC

GGT

However a large proportion are either polymorphic in some populations only, or at levels lower than good tri-allelic SNPs

Page 16: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

MH-15 99 nt

KHV JPT CHS CHB CDXTSI IBS GBR FIN CEU STU PJL ITU GIH BEB

YRI MSL LWK GWD ESNPEL

TAT

TAG

GGG

GAG

However a large proportion are either polymorphic in some populations only, or at levels lower than good tri-allelic SNPs

Page 17: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

MH-19 72 nt

TGT

TGG

TAG

KHV JPT CHS CHB CDXTSI IBS GBR FIN CEU STU PJL ITU GIH BEB

YRI MSL LWK GWD ESNPEL

GGG

However a large proportion are either polymorphic in some populations only, or at levels lower than good tri-allelic SNPs

Page 18: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

KHV JPT CHS CHB CDXTSI IBS GBR FIN CEU STU PJL ITU GIH BEB

YRI MSL LWK GWD ESNPEL

MH-8 35 nt

TG

TA

CG

CA

However a large proportion are either polymorphic in some populations only, or at levels lower than good tri-allelic SNPs

Page 19: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

A A C

G C T

A A T

G C C

A C C

G A T

A C T

G A C

G A C

A C T

G A T

A C C

G C C

A A T

G C T

A A C

AG, AC, CT

33 = 27 genotype combinations

82 = 64/2 32 haplotype combinations

Haplotype combinations offer potentially extensive levels of polymorphism compared to single-site SNPs

Page 20: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

AG, AC, CT A A C

G C T

Most Microhaplotypes represent a novel base change on an established allelic background that rises in frequency

A A T

G C C

A C C

G A T

A C T

G A C

G A C

A C T

G A T

A C C

G C C

A A T

G C T

A A C

33 = 27 genotype combinations

Here, the GCT haplotype predominates in the observed variation

82 = 64/2 32 haplotype combinations

Page 21: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

FP1!FP2!

• 1457 markers were incorporated after linkage screening - only 6 sites were eliminated based on primer extension disqualification

• 1377 autosomal tri-alleleic SNPs

• 34 tri-allelic X chromosome SNPs

• 46 microhaplotypes with 2, 3, 4, and 5 SNP combinations

• 2832 target enrichment extension primers - 80% of sites with redundant targeting

• 1195 markers currently under further evaluation

• 1123 autosomal tri-alleleic SNPs

• 28 tri-allelic X chromosome SNPs

• 44 microhaplotypes with 2, 3, 4, and 5 SNP combinations

Tri-allelic SNP and microhaplotype genotyping specificity is proving to be a major issue with the ICMP panel

Page 22: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Automated phasing - adapting the TFS prototype plugin

Page 23: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Automated phasing

Page 24: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Automated phasing

Page 25: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

• Many 2-SNP microhaplotypes are less polymorphic than the best tri-allelic SNPs (which have a better chance to be very short)

Microhaplotypes bring new aspects to forensic analyses

• Post MPS genotype data compilation steps need to automatically establish phase of component SNP alleles on each sequence strand

• During microhaplotype selection for the ICMP panel, low frequency SNPs observed within the microhaplotype bounds were simply ignored, but these could provide useful data

• Deletions within or close to the target microhaplotype are common, and may create difficulties for reliable alignment or create frameshift effects

• A wide range of haplotypes in the most polymorphic loci will need very large sample sizes to obtain reliable population frequencies (with the possible problem of estimating frequencies of novel, single observations)

• Some established single-site SNPs ‘adventitious’ haplotypes from flanking SNP

Page 26: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

The Qiagen Global AIMs+ and Middle East

informative AIMs panels

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Page 27: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

1000 Genomes continental population data informs all USC’s AIM SNP choice and analysis

Page 28: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

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Page 29: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Used the CEPH panel to find Eurasian-informative SNPs

Page 30: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

128 61The EUROFORGEN Global AIMs Panel

Qiagen Global AIMs+ 189 SNPs

EUROFORGEN Global ancestry informative SNPs, and QIAGEN’s extended set

extra AIMs

Page 31: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

46 64 100

The EUROFORGEN “NAME” Panel110 SNPs

Qiagen Middle East Panel 164 SNPs

31 EUR-ME SNPs

41 EUR-N AFR SNPs

21 EUR-N AFR SNPs

7 Eurasiaplex SNPs

Middle East informative SNPs in two sets under development

Page 32: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

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Britain Denmark Slovenia Albania Greece

Algeria Libya IraqAzerbaijan Morocco

Pakistan IndiaArabia AfghanistanKuwait

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AFR

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Page 33: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Simons Foundation provides 200 free complete genomes

Unlike 1000 Genomes, Simons Foundation samples 2-3 individuals per location with widest possible geographic spread

Illumina ancestry panel of 55 (Kiddlab)

Ion PGM ancestry panel of 169 AIMs (Kiddlab + Seldin/Kosoy)

Euroforgen ancestry panel of 128

Snipper

http://mathgene.usc.es/snipper/index.php

Page 34: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Snipper PCA analysis - MPS ancestry markers

PCA analysis can only work with binary SNP data - multiple allele (short sequence) data such as MHs needs genetic cluster analysis e.g. STRUCTURE - and we get much more population detail

Adapting exiting forensic ancestry analysis regimes to new marker sets

Page 35: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Snipper PCA analysis - MPS ancestry markers

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60%

70%

80%

90%

100%

121-17-M1 121-17-M2 121-17-M3 121-17-M4 121-17-M5 121-17-M6 121-17-M7 121-17-M8 121-17-M9 121-17-M10

SASN

AFR

OCE

EASN

AME

EUR

ME

K:7

Sub-Saharan Africa

S W E North

Afri

caM

iddle

East

Cauc

asus

-Far

East

Euro

peEa

stern

Eur

ope

Europe

SE Central W N Cent

ral A

sia-W

Midd

le Ea

stGu

jarat

i

Punja

bi

Beng

li

Sout

h-Ce

ntra

l

India

SE E

ast A

siaOc

eania

Mala

yaEast Asian

China Dai SE M

ainlan

d As

ia

NE Asia Amer

ica

South Asia Non-European Eurasian (NEE)

NEE Siberia- America

Japan

PCA analysis can only work with binary SNP data - multiple allele (short sequence) data such as MHs needs genetic cluster analysis e.g. STRUCTURE - and we get much more population detail

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 101010101010101010101010101010101010101010101010101010101010101010101010101010101010101010101011111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111121212121212121212121212121212121212121212121212121313131313131313131313141414141414141414141414141414141414141414141414141414141414141414141414141414141414141414141414141414141414151515151515151515151515151515151515151515151515151515151515151515151515151515151515151515151515151616161616161616161616161616161616161616161616161616161616161616161616161616161616161616161617171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717171717181818181819191919191919191919191920202020202121212121212222222222222222222222222222222222232323232323232323232323232323232323232323232323232323232323232323232323232323232323232323232323242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242424242525252525252525252525252525252525252525252525252525252525252525252525252525252525252525252525252525252525252526262626262626262626262626262626262626262626262626262626262626262626262626262626262626262626262627272727272727272727272727272727272727272727272727272727272727272727272727272727272727272727272727272727272727272728282828282828282828282828282828282828282828282828282828282828282828282828282828282828282828282828282828282828282828282828282828292929293030303030303030303030303030303030303030303030303031313131313232323232323232

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

121-17-M1 121-17-M2 121-17-M3 121-17-M4 121-17-M5 121-17-M6 121-17-M7 121-17-M8 121-17-M9 121-17-M10

SASN

AFR

OCE

EASN

AME

EUR

ME

K:7

Sub-Saharan Africa

S W E North Africa

Middle East

Caucasus-Far

East Europe

Eastern Europe

Europe

SE Central W N Central Asia-W

Middle East

Gujarati

Punjabi

Bengli

South-Central

India

SE East Asia

Oceania

Malaya

East Asian

China Dai SE Mainland Asia

NE Asia America

South Asia Non-European Eurasian (NEE)

NEE Siberia- America

Japan

Page 36: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Concluding remarks - evolving systems of forensic MPS analysis

Not all markers will work well - alignment, close linkage and lack of specificity all affect the usefulness of MPS genotype data

As MPS is costly and work-intensive, generating population data is going to be slow and restricted - microhaplotype variation will need much bigger data scales than SNPs

STRUCTURE handles all types of genetic variant data so should be the system of choice for forensic ancestry analysis to maximise the informativeness of all genotypes

The development of an MPS test for forensic purposes needs time and care as well as detailed scrutiny of genotyping performance - best made in collaborative frameworks

Page 37: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Yale!"#$%$#&!'

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[email protected]

mailto:[email protected]
Page 38: Forensic SNP typing with QIAGEN’s QIAseq NGS chemistry · •140-SNP Identification panel Qiagen SNP panel developments and some key issues: adapting to MPS and new multiple-allele-short-sequence

Yale!"#$%$#&!'

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SNP comparisons

SNP comparisons

SNP Analysis

SNP Analysis

QIAseq Technologies for Metagenomics and Microbiome NGS Library Prep

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Snp Catalog

Snp Catalog

SNP technologies

SNP technologies

COMPANY€¦ · SNP EasyVAT, SNP EasyCIT – tax returns automatically from SAP SNP EasyExchangeRates – automatic transfer of exchange rates to SAP SNP Standard Audit File in SAP

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Fish scales and SNP chips: SNP genotyping and allele frequency ...

Fish scales and SNP chips: SNP genotyping and allele frequency ...

QIAseq™ Library Quant Assay Kit

QIAseq™ Library Quant Assay Kit

SNP Selection

SNP Selection

Illumina SNP Genotyping Technologies BeadArray and ...barleyworld.org/sites/barleyworld.org/files/illuminasnpgenotyping.pdf · SNP Genotyping Assay and SNP Multiplexing Level per

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SNP-l5233H/l5233€¦ · Security Dimensions (WxH) Weight SNP-l5233H/l5233 SNP-l5233HN/HP SNP-l5233N/P 1.3M HD 23x Network PTZ Dome Camera SNP-L5233H SNP-L5233 key Features

SNP-l5233H/l5233€¦ · Security Dimensions (WxH) Weight SNP-l5233H/l5233 SNP-l5233HN/HP SNP-l5233N/P 1.3M HD 23x Network PTZ Dome Camera SNP-L5233H SNP-L5233 key Features

Gene-Gene /SNP-SNP Interaction: BIOFILTER

Gene-Gene /SNP-SNP Interaction: BIOFILTER

QIAseq 16S/ITS Panels

QIAseq 16S/ITS Panels

Saurav snp

Saurav snp

Improving Microbiome Sequencing using QIAseq 16S/ITS Panels · 4 Improving Microbiome Sequencing using QIAseq 16S/ITS Panels 08/2018 kit but on separate runs. Paired-end sequencing

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Outline to SNP bioinformatics lecture Brief introduction SNPs in cell biology SNP discovery SNP assessment SNP databases SNPs in genome browsers.

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QIAseq 16S/ITS Panel Handbook

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Special Needs Plan (C-SNP/D-SNP) - MedStar Provider Networkmedstarprovidernetwork.org/sites/default/files/attachments/MedStar... · Special Needs Plan (C-SNP/D-SNP) Objectives Upon

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QIAseq UPX 3' Targeted RNA Library Handbook

QIAseq UPX 3' Targeted RNA Library Handbook

PTZ/Dome Mounts...SCP-2370TH/2370H SCP-2370RH/2330H SCP-2273H/2271H SCP-2270H/2250H SNP-6321H/6320H/6201H SNP-6200RH/6200H SNP-5430H/5321H SNP-5300H/5200H SNP-3371TH/3371H SNP-3302H/3120VH

PTZ/Dome Mounts...SCP-2370TH/2370H SCP-2370RH/2330H SCP-2273H/2271H SCP-2270H/2250H SNP-6321H/6320H/6201H SNP-6200RH/6200H SNP-5430H/5321H SNP-5300H/5200H SNP-3371TH/3371H SNP-3302H/3120VH