Insightful solutions for Biologics Drug Discovery with a focus on Monoclonal Antibodies (mAb)

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Sartorius Solutions for Monoclonal Antibodies (mAb) Discovery and Development

Biomolecular Interaction Analysis Platforms for Antibody Discovery and Cell Line Development

Gain More Insights, Make Better Decisions

Therapeutic antibodies have recently emerged as one of the most successful strategies for the treatment of both hematologic cancers and solid tumors. Because they offer the promise of better specificity and safety than many other cancer treatments, this class of biologics is projected to continue increasing in importance.

Octet® systems are used for affinity ranking, epitope binning of small to large antibody matrices, Fc-receptor binding, cross-reactivity testing of other species, glycosylation screening, characterization of antibody-antigen binding

kinetics and affinity, bispecific testing, and titer analysis for protein expression, all in one high throughput, easiest-to-use system.

Accelerate Your Antibody Development and Still Have Peace of Mind

For development of breakthrough therapeutics you need revolutionary technologies providing rapid, multifactorial results to enable deeper insights and ensure the choice, quality, and integrity of your biopharmaceutical product.

We do this by enabling - Process streamlining- Multiplexing- Acquisition of information-rich data - More meaningful and biologically relevant insights

See how our unique and innovative technologies and solutions ensure your confidence in the results of your research and the quality of your product at every stage of the biologics discovery and development workflow.

Features Image Value

Antibody and glycosylation titer using crude samples

- Significantly reduce time to develop antibodies by combining titer and glycan screening- Save FTE costs and perform more projects using the high-throughput Octet® system

Affinity determination and kinetic characterization

- Assess the binding characteristics of your biologics molecules in real time - Accurately determine association and dissociation rate constants for your biomolecular interactions studies

Epitope binning and cross reactivity test using crude samples

- NEW BinChart for convenient binning of antibodies- Advanced settings for fitting quantitation datasets- Optimal visualization via overlaid fitted traces on top of raw quantitation binding traces- NEW capability for reference subtraction within and across plates- Optimized Report Tab for direct creation of PowerPoint/PDF reports

Offrate screening using crude samples

Rapid antibody off rates screening using crude samples Complete with comprehensive analysis and visualization capabilities, our platforms will empower you with actionable results, faster!

Antibody Library ScreeningIncrease data throughput and quality by multiplexing antibody binding, specificity, and species cross reactivity.

ProliferationMeasure cell proliferation in mono- or co-cultures, with or without labels.

3D Tumor Spheroid AssaysTrack and quantify tumor spheroid formation, growth, and death in real time.

Functional ProfilingEasily go from high-content functional assays to actionable information that helps you select candidates with desirable biological activity.

ApoptosisInvestigate aberrations in apoptotic pathways in intact, living cells.

Cell Migration and InvasionMonitor and analyze migration and invasion with or without a chemotactic gradient.

Cell Line DevelopmentSelect antibody clones upstream of cell line development by simultaneously detecting IgG titer, cell health and viability.

CytotoxicityDetect real-time measurements of cell death based on cell membrane integrity.

Antibody InternalizationQuantify antibody internalization in 96- or 384-well formats.

The Incucyte® Live-Cell Analysis, iQue® Advanced Cytometry Flow and Octet® Label-Free Analysis systems offer unique, high-capacity cell analysis solutions for characterizing cell phenotype and function and tracking complex biological processes.

Legend:

Cell Analysis Platforms for Tracking Complex Biological Processes

iQue® Incucyte® Octet®

Find out more For more information, please visit www.sartorius.com/mAb-oncology

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1Incucyte® Live-Cell Analysis System Incucyte® Live-Cell Analysis System

Octet® Label-Free Bio-Layer Interferometry (BLI) Detection Systems

ambr® 15 Bioreactor System Cell Line, Media & Testing ServiceVivaspin® Ultrafiltration Devices and Vivapure® Virus Purification Kits

Rapid Contamination control – Microsart® Mycoplasma & Bacteria

Picus® NxT Electronic Pipette

iQue® Platform | Cy-Clone™ PLUS assay

iQue® Advanced Flow Cytometry Platform

Molecule Development

Target identification Gene cloning and screeningLibrary screening Cell line selection | identificationLead optimization Cell line characterizationCandidate characterization Cell banking

Cell Line Development

3 Octet® Label-Free Bio-Layer Interferometry (BLI) Detection Systems

Multiplexed screening assays Binding assays Functional assays Functional assaysCy-Clone™ PLUS assays

8 Sartoclear Dynamics® Lab

8 Sartoclear Dynamics® Lab

iQue® Advanced Flow Cytometry PlatformA high-throughput, suspension cell and bead-based analysis platform for antibody screening and functional profiling. The fastest way to generate high content data from small sample volumes.- Screen your libraries in days,

not months- Sample volumes as low as 1 µL, reduce reagent costs- Screen cells in suspension and tar-gets in their natural conformation

See specific applications in “Cell Analysis Platforms“!

Incucyte® Live-Cell Analysis SystemEnables real-time, live-cell imaging and analysis directly inside your incubator. - Use with non-perturbing Incucyte®

Reagents to detect dynamic bio-logical changes - Measure kinetic effects of your antibody treatment on target cells - Accommodates multiple users and applications in parallel

See specific applications in“Cell Analysis Platforms“!

Microsart® Mycoplasma & Bacteria qPCR kits offer a rapid, reliable and easy-to-use solution for microbial quality control in compliance with international guidelines.- Don’t worry about false positives

due to highly specific TaqMan® probes- Let the Master Mix do the internal control and reduce tedious pipetting steps- Eliminate contamination risks by using the only non-infectious CFU standards

Octet® Label-Free Bio-Layer Interferometry (BLI) Detection Systems High throughput, easiest-to-use systems for affinity ranking, epitope binning of small to large antibody matrices, Fc-receptor binding, cross-reactivity testing of other species, glycosylation screening, characterization of antibody- antigen binding kinetics and affinity, bispecific testing, and titer analysis for protein expression

Picus® is the lightest and smallest electronic pipette on the market. Its ergonomic design allows comfortable handling to ease the workload of laboratory professionals in long pipetting series.- Fully electronic operation for

consistent, user independent results- The extensive range of pipetting modes makes pipetting tasks quick and easy- Features like programmable protocols and plate tracker reduce the risk of user errors

ambr® 15 Bioreactor System is a high-throughput automated micro bioreactor for predictive cell line and media screening. Rapidly evaluate multiple bio reactor cultures at microscale, thus increasing your productivity in cell line development and saving on materials and labor.- Rapid screening of clones and media

optimization- Data-rich culture profiles with automated sampling- Large multifactorial experimental designs (DoE) in real time

Sartorius Cell Line, Media & Testing Service delivers high-yielding, stable cell lines and robust, simple, fed-batch processes for the biopharmaceutical market.- The Cellca CHO Expression Platform

is an easily accessible, scalable, and robust system- Delivery from DNA to high-titre Research Cell Banks in 4 – 5 months- Reduced cost of goods, develop-ment risks and development time

Vivaspin® Centrifugal Concentrators provide near total protein, virus and other nanoparticle concentrations in times as quick as under ten minutes. Now with the unique offering of both PES and RC membranes to optimize molecule to membrane compatibility. - Unique PES and RC membrane

range - Market leading concentration times- Angular dead stop for complete sample aspiration- Market leading technical and appli-cation support

Sartoclear Dynamics® Lab is a new method for harvesting mammalian cell cultures in one-step filtration without any centrifugation for clarification.- Time saving because of singlestep

filtration, no setup required, and high flow rates- No lengthy centrifugation step needed- Efficient, as only one filter needed- Convenient, as hand-free filtration of up to 6 samples in parallel

iQue® Advanced Flow Cytometry Platform with Cy-Clone™ PLUS Assay Clone ranking and selection of production clones with rapid and simultaneous evaluation of IgG titer, specific productivity, and cell health.- More insight for better clone

selection- More critical productivity attributes from each clone- More clones screened in less time

See specific applications in “Cell Analysis Platforms“!

Our Solution for High Content Antibody Screening

Our Solution for Real-Time, Live-Cell Imaging

Our solution for real-time, label free quantitative and kinetic analysis

Our Solution for Ergonomic Pipetting

Our Solution for High- Throughput Cell Line Development

Our Solution for Cell Line Development and Cell Banking Services

Our Solution for Molecule Concentration

Our Solution for Cell Culture Harvesting

Our Solution for Cell Line Development

Our Solutions for Rapid Contamination Control

Multifactorial and actionable results for more insight and better decisions

See what your cells are doing and when

Reduce time-to-result significantlyPerform your protein quantitation and kinetics characterization assays at unmatched speed and throughput

Achieve accurate results, time after time

Improve the overall efficiency and speed of your cell line and media development by 90%

Benefit from our extensive experience in custom cell line development projects

Quickly and reliably concentrate your molecules to the desired concentration factor

Quickly and easily harvest clarified supernatant containing your target molecule

Quickly and efficiently learn critical productivity attributes for each clone

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Sales and Service ContactsFor further contacts, visit www.sartorius.com

Germany Sartorius Lab Instruments GmbH & Co. KG Otto-Brenner-Strasse 20 37079 Goettingen Phone +49 551 308 0

USA Sartorius Corporation 5 Orville Drive, Suite 200 Bohemia, NY 11716 Phone +1 631 254 4249 Toll-free +1 800 635 2906

Specifications subject to change without notice. Copyright Sartorius Lab Instruments GmbH & Co. KG. Version 08 | 2020