Food Safety / Analytic Kits · FIS-E01 Fish (Parvalbumin) 270 GLU-E02 Gluten / Gliadin 270 HAZ-E01...
Transcript of Food Safety / Analytic Kits · FIS-E01 Fish (Parvalbumin) 270 GLU-E02 Gluten / Gliadin 270 HAZ-E01...
Food Safety / Analytic Kits (Price List 2014 • Distributors)
Immunolab
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Immunolab GmbH fon +49 (0)561 491 742-0 mail [email protected] Otto-Hahn-Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742-20 web www.immunolab.de
PREL-FA_dis_eng_v3
Food ELISA (96 Tests)
Cat. No. Product Name Price (EUR) 1
Mycotoxins
AB1-E01 Aflatoxin B1 195
AM1-E01 Aflatoxin M1 250
DON-E01 Deoxynivalenol (DON) 230
DON-E03 Deoxynivalenol (DON) RAPID 230
FUM-E01 Fumonisin 230
FUM-E03 Fumonisin RAPID 230
OTA-E01 * Ochratoxin A 230
ZEA-E01 Zearalenone 230
ZEA-E03 Zearalenone RAPID 230
T2T-E01 T2-Toxin 230
Vitamins
FOL-E01 Folic Acid 270
B12-E01 Vitamin B12 270
BIO-E01 Vitamin H (Biotin) 270
Antibiotics
CAP-E02 Chloramphenicol 230
PEN-E01 Penicillin 250
STR-E02 Streptomycin 250
TCY-E01 * Tetracycline upon request
Hormones
EST-E01 Estradiol 195
PRO-E01 Progesterone 195
TES-E01 Testosterone 195
Miscellaneous
HIS-E02 Histamine 270
HIS-E03 Histamine RAPID 270
* in development
Food Safety / Analytic Kits (Price List 2014 • Distributors)
Immunolab
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Immunolab GmbH fon +49 (0)561 491 742-0 mail [email protected] Otto-Hahn-Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742-20 web www.immunolab.de
PREL-FA_dis_eng_v3
Food ELISA (96 Tests) continuation
Cat. No. Product Name Price (EUR) 1
Allergens
ALM-E01 Almond 270
BLG-E01 Beta-Lactoglobulin 270
CAS-E01 Casein 270
CAW-E01 Cashew 270
CEL-E01 * Celery 270
CRU-E01 Crustaceans (Tropomyosin) 270
EGG-E01 Egg White 270
FIS-E01 Fish (Parvalbumin) 270
GLU-E02 Gluten / Gliadin 270
HAZ-E01 Hazelnut 270
LUP-E01 Lupine 270
LYS-E01 Lysozyme 270
MIL-E01 Milk 270
MUS-E01 Mustard 270
OVA-E01 Ovalbumin 270
PEA-E01 Peanut 270
PEC-E01* Pecan Nut 270
PIS-E01 Pistachio 270
SES-E01 Sesame 270
SOJ-E01 Soja (Soy) 270
WAL-E01 Walnut 270
* in development
Food Immunoaffinity Columns (10 columns / kit)
Cat. No. Product Name Price (EUR) 1
AFB-A01 Aflatoxin (total) 65
AB1-A01 Aflatoxin B1 65
B12-A01 Vitamin B12 95
BIO-A01 Vitamin H (Biotin) 95
ZEA-A01 Zearalenone 80
1 All prices are given in EURO, net ex works, exclusive of packaging.
Food Intolerance ELISA Tests (Price List 2014 • Distributors)
Immunolab
Immunolab GmbH fon +49 (0)561 491 742-0 mail [email protected] Otto-Hahn-Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742-20 web www.immunolab.de
PREL-FI_dis_eng_v2
Cat. No. Product Name Price (EUR) 1
Food IgG ELISA 2
ILE-SCG25 Specific IgG Screening, Nutritional, 3x 24 food antigens 80
ILE-SCG26 Specific IgG MultiScreen, Nutritional, 1x 88 food antigens 80
Food IgG4 ELISA 2
ILE-SCG06 Specific IgG4 Screening, Nutritional, 4x 20 food antigens 80
ILE-SCG80 Specific IgG4 MultiScreen, Nutritional, 1x 88 food antigens 80
ILE-SCG210 Specific IgG4 Screening, Nutritional, 3x 24 food antigens 80
Coeliacal Antibodies
ILE-GLI01 Gliadin / Gluten IgG, 96 tests 90
ILE-GLI02 Gliadin / Gluten IgA, 96 tests 100
ILE-GLI03 Gliadin / Gluten IgM, 96 tests 100
1 All prices are given in EURO, net ex works, exclusive of packaging.
2 For Food IgG and IgG4 various formats (number of food antigens per microtiter plate) and
invidual, regional specific food antigen compositions can be ordered.
Infectious Disease ELISA Kits (96 Tests) (Price List 2014 • Distributors)
Immunolab
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Immunolab GmbH fon +49 (0)561 491 742-0 mail [email protected] Otto-Hahn-Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742-20 web www.immunolab.de
PREL-IN_dis_eng_v3
Cat. No. Product Name Price (EUR) *
ILE-ADV01 Adenovirus IgG 90
ILE-ADV02 Adenovirus IgA 100
ILE-ADV03 Adenovirus IgM 100
ILE-ASP01 Aspergillus fumigatus IgG 80
ILE-ASP02 Aspergillus fumigatus IgA 90
ILE-ASP03 Aspergillus fumigatus IgM 90
ILE-BPT01 Bordetella pertussis IgG 80
ILE-BPT02 Bordetella pertussis IgA 90
ILE-BPT03 Bordetella pertussis IgM 90
ILE-BPT04 Bordetella pertussis Toxin IgG 110
ILE-BPT07 Bordetella pertussis FHA IgG 110
ILE-BOR01 Borrelia burgdorferi IgG 90
ILE-BOR03 Borrelia burgdorferi IgM 100
ILE-BRU01 Brucella abortus IgG 90
ILE-BRU02 Brucella abortus IgA 100
ILE-BRU03 Brucella abortus IgM 100
ILE-CAN01 Candida albicans IgG 80
ILE-CAN02 Candida albicans IgA 90
ILE-CAN03 Candida albicans IgM 90
ILE-CMV01 Cytomegaly IgG 60
ILE-CMV03 Cytomegaly IgM 70
ILE-DIP01 Diphtheria IgG (Corynebacterium diphtheriae) 100
ILE-EBA01 Epstein-Barr Early Antigen (EA) IgG 90
ILE-EBA02 Epstein-Barr Early Antigen (EA) IgA 100
ILE-EBA03 Epstein-Barr Early Antigen (EA) IgM 100
ILE-EBV01 Epstein-Barr Nuclear Antigen (EBNA) IgG 90
ILE-EBV02 Epstein-Barr Nuclear Antigen (EBNA) IgA 100
ILE-EBV03 Epstein-Barr Nuclear Antigen (EBNA) IgM 100
ILE-EBC01 Epstein-Barr Virus Capsid Antigen (VCA) IgG 90
ILE-EBC02 Epstein-Barr Virus Capsid Antigen (VCA) IgA 100
ILE-EBC03 Epstein-Barr Virus Capsid Antigen (VCA) IgM 100
Infectious Disease ELISA Kits (96 Tests) (Price List 2014 • Distributors)
Immunolab
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Immunolab GmbH fon +49 (0)561 491 742-0 mail [email protected] Otto-Hahn-Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742-20 web www.immunolab.de
PREL-IN_dis_eng_v3
Cat. No. Product Name Price (EUR)
ILE-ECH01 Echinococcus granulosus IgG 90
ILE-FSM01 TBEV (FSME) IgG 100
ILE-FSM03 TBEV (FSME) IgM 110
ILE-HEL01 Helicobacter pylori IgG 80
ILE-HEL02 Helicobacter pylori IgA 90
ILE-HEL03 Helicobacter pylori IgM 90
ILE-HS101 Herpes 1 IgG 80
ILE-HS102 Herpes 1 IgA 90
ILE-HS103 Herpes 1 IgM 90
ILE-HS201 Herpes 2 IgG 80
ILE-HS202 Herpes 2 IgA 90
ILE-HS203 Herpes 2 IgM 90
ILE-HSC01 Herpes 1&2 IgG 80
ILE-HSC02 Herpes 1&2 IgA 90
ILE-HSC03 Herpes 1&2 IgM 90
ILE-IFA01 Influenza A IgG 80
ILE-IFA02 Influenza A IgA 90
ILE-IFA03 Influenza A IgM 90
ILE-IFB01 Influenza B IgG 80
ILE-IFB02 Influenza B IgA 90
ILE-IFB03 Influenza B IgM 90
ILE-MAS01 Measles IgG 80
ILE-MAS02 Measles IgA 90
ILE-MAS03 Measles IgM 90
ILE-MJS01 MeljaSorb (RF Absorbent) 40
ILE-MUM01 Mumps IgG 80
ILE-MUM02 Mumps IgA 90
ILE-MUM03 Mumps IgM 90
Infectious Disease ELISA Kits (96 Tests) (Price List 2014 • Distributors)
Immunolab
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Immunolab GmbH fon +49 (0)561 491 742-0 mail [email protected] Otto-Hahn-Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742-20 web www.immunolab.de
PREL-IN_dis_eng_v3
Cat. No. Product Name Price (EUR)
ILE-MYK01 Mycoplasma pneumoniae IgG 90
ILE-MYK02 Mycoplasma pneumoniae IgA 100
ILE-MYK03 Mycoplasma pneumoniae IgM 100
ILE-PAX01 Parainfluenza 1, 2 & 3 IgG 80
ILE-PAX02 Parainfluenza 1, 2 & 3 IgA 90
ILE-PAX03 Parainfluenza 1, 2 & 3 IgM 90
ILE-POL01 Poliomyelitis IgG 110
ILE-RSV01 Respiratory syncytial virus (RSV) IgG 90
ILE-RSV02 Respiratory syncytial virus (RSV) IgA 100
ILE-RSV03 Respiratory syncytial virus (RSV) IgM 100
ILE-RUB01 Rubella IgG 60
ILE-RUB03 Rubella IgM 70
ILE-TET01 Tetanus IgG (Clostridium tetani) 100
ILE-TOX01 Toxoplasma IgG 60
ILE-TOX03 Toxoplasma IgM 70
ILE-TUB01 Tuberculosis IgG (Mycobacterium tuberculosis) 120
ILE-TUB02 Tuberculosis IgA (Mycobacterium tuberculosis) 140
ILE-TUB03 Tuberculosis IgM (Mycobacterium tuberculosis) 140
ILE-TUS01 Tuberculosis IgG sensitive (Mycobacterium tub.) 120
ILE-TUS02 Tuberculosis IgA sensitive (Mycobacterium tub.) 140
ILE-TUS03 Tuberculosis IgM sensitive (Mycobacterium tub.) 140
ILE-VAR01 Varicella zoster IgG 80
ILE-VAR02 Varicella zoster IgA 90
ILE-VAR03 Varicella zoster IgM 90
ILE-YER01 Yersinia enterocolitica IgG 80
ILE-YER02 Yersinia enterocolitica IgA 90
ILE-YER03 Yersinia enterocolitica IgM 90
* All prices are given in EURO, net ex works, exclusive of packaging.
Food Safety / Analytic Kits
Newsletter Allergen ELISA Kits Immunolab
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected] Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
PRIN-FA_allergens_eng_v5
Food Allergy is a rapidly increasing area of consumer concern. Food allergens are proteins in food that, even in low concentrations, can trigger allergic, sometimes life‐threatening reactions in sensitive individuals.
As protection of the consumer, the EU directive 2003/89/EC was issued in November 2005. It obliges the food manufacturers to label their products with all ingredients capable of causing an allergic reaction. Annex IIIa includes a list of food ingredients classified as being possible allergens or intolerants, e.g. peanuts, hazelnuts, eggs, gluten, almond, milk, soy, crustaceans and celery.
Testing for the presence of food residues provides security for food manufacturers that a potentially dangerous ingredient is not included in their food products.
Immunolab GmbH as an acknowledged developer and manufacturer of high‐quality Food Safety/Analytic and Clinical Diagnostic products, hereby introduces its new food allergen product line to detect almond, beta‐lactoglobulin, casein, crustaceans, egg white, fish, gluten, hazelnut, lupine, mustard, peanut, soy and walnut residues in raw materials and processed foods. Further parameters (Celery, Fish) are in development. The Immunolab food allergen kits are 96 well ELISA tests and can be processed according to a usual standard sandwich ELISA protocol. Each kit includes all components required to perform the assay. Features and Benefits • Easy Handling by:
o Standardized Test Protocol o Short Assay Time, 60 minutes (20/20/20 min) o Reagents (excl. wash buffer) ready‐to‐use; breakable strips o Simple extraction procedure and no further clean‐up steps o Incubation at ambient temperature
• High Accuracy and Reproducibility • High Stability, minimum shelf life 12 months
Technical Performance Data
Analyte LOD LOQ Ref. Range Recovery Precision (intra‐assay)
Almond 0.2 ppm 0.4 ppm 0 – 10 ppm 67 – 109 % 3 – 9 %
Beta‐Lactoglobulin 1.5 ppb 10 ppb 0 – 400 ppb 70 – 107 % 7 %
Casein 0.04 ppm 0.2 ppm 0 – 6 ppm 80 – 102 % 5 ‐ 11 %
Celery * available soon available soon available soon available soon available soon
Crustaceans (Tropomyosin) 0.9 ppb 20 ppb 0 – 400 ppb 84 – 97 % 6 – 8 %
Egg White 0.05 ppm 0.4 ppm 0 – 10 ppm 82 – 98 % 4 – 9 %
Fish (Parvalbumin) * available soon available soon available soon available soon available soon
Gluten / Gliadin 0.3 ppm 2 ppm 0 – 60 ppm 85 – 98 % 4 – 5 %
Hazelnut 0.3 ppm 1 ppm 0 – 40 ppm 83 – 101 % 7 – 12 %
Lupine 0.2 ppm 2 ppm 0 – 30 ppm 98 – 113 % 6 – 9 %
Mustard 1 ppm 2 ppm 0 – 60 ppm 76 – 98 % 8 %
Peanut 0.1 ppm 1 ppm 0 – 40 ppm 90 – 110 % 7 – 10 %
Sesame 0.2 ppm 2 ppm 0 – 30 ppm 85 – 110 % 5 – 12 %
Soja (Soy) 16 ppb STI 40 ppb STI 0 –1000 ppb 77 – 122 % 6 – 8 %
Walnut 0.35 ppm 2 ppm 0 – 60 ppm 75 – 106 % 4 – 9 %
Food Safety / Analytic ELISA Mycotoxins Cat. No. Aflatoxin B1 AB1‐E01 Aflatoxin M1 AM1‐E01 Deoxynivalenol (DON) * DON‐E01 Fumonisin FUM‐E01 Ochratoxin A * OTA‐E01 Zearalenone ZEA‐E01
Antibiotics Chloramphenicol CAP‐E02 Penicillin PEN‐E01 Streptomycin STR‐E02 Tetracycline * TCY‐E01
Vitamins Folic Acid (Vit. B9) FOL‐E01 Vitamin B12 B12‐E01 Biotin (Vitamin H) BIO‐E01
Hormones Estradiol EST‐E01 Progesterone PRO‐E01 Testosterone TES‐E01
Miscellaneous Histamine HIS‐E02 Gluten / Gliadin GLU‐E01
Allergens Almond ALM‐E01 Beta‐Lactoglobulin BLG‐E01 Casein CAS‐E01 Celery * CEL‐E01 Crustaceans CRU‐E01 Egg white EGG‐E01 Fish (Parvalbumin) * FIS‐E01 Gluten / Gliadin GLU‐E02 Hazelnut HAZ‐E01 Lupine LUP‐E01 Mustard MUS‐E01 Peanut PEA‐E01 Sesame SES‐E01 Soja (Soy) SOJ‐E01 Walnut WAL‐E01
* in development
Food Intolerance Tests » For a Better Quality of Life « Immunolab Food Antigens
page 1 of 2 PRIN-FI antigens_eng_v3 [Revision: June 2011]
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected]
Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
agar
agave
alfalfa
allspice
almond
aloe vera
amaranth
anchovy
angler
anise
appenzeller
apple
apricot
artichoke
asparagus
avocado
baker's yeast
baking powder
bamboo
banana
barley
basil
batata
bay leaf
bear's garlic
beef
beetroot
bell pepper
black currant
black eyed pea
black radish
black salsify
blackberry
blue mold cheese
blue mussel
blueberry
borage
brazil nut
brewer's yeast
broad bean
broccoflower
broccoli
brook trout
brussels sprout
buck's horn plantain
buckwheat
butter
buttermilk
button mushroom
calf's liver
camel milk
camembert
cantaloupe, north amer.
canderel
cape gooseberry
caper
carambola
caraway
cardamom
carob
carp
carrageenan
carrot
casein
cashew nut
cauliflower
caviar black
caviar red
cayenne pepper
celeriac
celery
cep
chamomile
chanterelle
chard
cheddar
cherimoya
cherry
chervil
chestnut
chick pea
chicken
chicory
chili
chinese cabbage
chive
chlorella
cinnamon
clementine
clove
coalfish
cocoa
coconut
coconut milk
cod
coffee
coke
common shrimp
coriander
corn salad
cottage cheese
courgette
cowberry
cow's milk
cranberry
crayfish
cream
cream cheese
cucumber
cumin
curd
curry
cuttlefish
damson
dandelion
dark chocolate
date
desert indianwheat
dill
duck
duck egg
east african land snail
edam
eddo
edible snail
eel
egg replacement powder
egg white
egg yolk
eggplant
elderberry
emmental
emmer
endive
European cantaloupe
european perch
fallow deer
fennel
fig
flax seed
flounder
garden cress
garlic
gelatin
gilthead seabream
gin
ginger
gliadin
glutamate
goat
goat cheese
goat's milk
golden syrup
goose
gooseberry
goose egg
gouda
granadilla
grape red
grape white
grapefruit
graviera
gruyere cheese
guar gum
guava
gum arabic
gum karaya
haddock
hake
halibut
halloumi
hare
haw
hawthorn
hazelnut
hemp flour
herring
honey acacia
honey clover
honey rape
honeydew melon
hop
horse
horseradish
horta
hot pepper green
hot pepper red
iceberg lettuce
indian fig
iridescent shark‐catfish
jenever
jerusalem artichoke
juniper berry
kaki
kale
kamut
kapuisto tiri
kaseri
kefalotyrh
kefir
kiwano
kiwi
kolanut
kumquat
α‐lactalbumin
lactoferrin
β‐lactoglobulin
lamb
lavender
lecithin
leek
leerdam cheese
lemon
lemon balm
lemon soda
lemon verbena
lemongrass
lentil
lettuce
lime
lime blossom
liquorice
lobster
locust bean gum
lollo rosso
Food Intolerance Tests » For a Better Quality of Life « Immunolab Food Antigens
page 2 of 2 PRIN-FI antigens_eng_v3 [Revision: June 2011]
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected]
Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
lovage
lukumia
lupin
lychee
lysozyme
macadamianut
mackerel
maize
maize meal
mallow
malt
mandarin
mangelwurzel
mango
mangosteen
manioc
maple syrup
mare's milk
marigold
marjoram
marzipan
mate
milk chocolate
millet
mirabelle plum
mistletoe leave
mistletoeberry
morel
mozzarella
mugwort
mung bean
mustard seed
mustard, brown
mustard, green
mustard, white
nashi
nectarine
nile perch
nile tilapia
noni
nori
nutmeg
oat
octopus
okra
olive black
olive green
olive oil
onion
orange
orange soda
oreagno
ostrich
ovalbumin
ovomucoid
ovotransferrin
oyster
oyster mushroom
palm heart
palm oil
papaya
paprika
parmesan
parsley
parsley root
parsnip
passion fruit
pastirma
pea
peach
peanut
pear
pecan nut
pectin
pepino
pepper black
pepper green
pepper white
peppermint
pheasant
pigeon
pike
pine nut
pineapple
pinto bean
pistachio
pitaya yellow
plaice
plantain
plum
pointed cabbage
pomegranate
pomelo
poppy seed
pork
potato
prawn
processed cheese
pumpkin
pumpkin seed
quail
quail egg
quince
quinoa
rabbit
radicchio
raisin
rape seed
raspberry
red bean
red cabbage
red currant
red deer
red mullet
red radish
red snapper
red tea
reindeer
retsina
rhubarb
rice
ricotta
rocket
romaine lettuce
roquefort
rosefish
roselle
rosemary
rum
rye
safflower oil
saffron
sage
Saint John's wort
salmon
sardine
sauerkraut
savory
savoy cabbage
scallop
sea bass
sea lamprey
seabuckthornberry
semolina
sesame
shallot
sheathed woodtuft
sheep's milk
sheep's milk cheese
shiitake
shrimps
sloe
snap pea
sole
sorrel
sour cherry
soy grain
soybean
soymilk
spelt
spinach
spined loach
spiny lobster
spirulina
squid
starch
sticky bun
stinging nettle
strawberry
string bean
sugar beet molasses
sultana
sunflower seed
swedish turnip
swordfish
tapioca
tarragon
tea black
tea green
tea white
teff
thyme
tiger nut
tilsit cheese
tobacco
tofu
tomato
tragacanth
triticale
trout
tuna
turbot
turkey
turmeric
turnip cabbage
turshi
unripe spelt grains
valerian
vanilla
veal
venison
venus clam
wakame
walnut
watermelon
wels catfish
wheat
whey
white bean
white cabbage
white chocolate
whole egg
wild boar
wild rice
wine leave
wine red
wine white
woodruff
wormwood
yam
yellow bean
yogurt
xanthan gum
zander
Food Intolerance Tests » For a Better Quality of Life «
Relevance of the detection of specific IgG / IgG4 Immunolab antibodies in the diagnosis of food intolerances
Page 1
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected] Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
PRIN-FI_background_eng_v1
There is increased clear epidemiological evidence during recent years that more and more people suffer from food intolerance symptoms. An analogous tendency is also observed for classical allergic diseases (IgE mediated, type 1, immediate reaction). The use of IgG and/or IgG4 tests to detect the triggering food antigens is still a controversially discussed subject among medical experts. Despite this situation the detection of IgG/IgG4 antibodies should always be considered if typical causes can be excluded like lactose or fructose malabsorption, celiac disease or an IgE mediated food allergy (type 1).
The allergic immediate reaction (type 1) is associated with a Th2 cell activation. Resulting from both the allergen portal of entry and exposure time the Th2 activation can switch to a more Th1 directed reaction leading to the formation of IgG4 and IgA antibodies. If this effect increases further towards Th1, IgG1 antibodies can be produced.
Specific IgG antibodies can be found in the serum of food allergy patients as well. In fact, numerous studies have described higher IgG4 levels in patients with IgE mediated symptoms (type 1) compared to non allergic individuals. The extent of the allergen exposure and exposure time have a strong impact on the concentration of IgG4 antibodies. This leads to the conclusion that an IgG4 reaction cannot be regarded as a physiological reaction only but has a pathological significance from a certain reactivity level.
There are four IgG subclasses of which IgG1 and IgG4 play a particular role in food intolerances. IgG1 is produced as initial response after contact with a new food antigen. After further exposure to the antigen a switch occurs towards formation of IgG4 antibodies
(1).
The IgG4 portion of total IgG can increase from 3‐5% to 50%. The elevated IgG4 concentration points to an intense conflict between the immune system and the triggering food antigen. Functionally, clear evidence could be obtained that IgG4 antibodies are able to sensitize cells temporarily but can block the allergic response as well. In fact 50% of untreated allergic patients show elevated IgG4 levels. Often disorders appear after hours only, causing difficulties to identify the triggering food. In many cases a disturbed intestinal permeability is postulated to initiate the food intolerance. This leads to a permanent exposure of the immune system by antigenic structures. The overload of the immune system can result in an enhanced sensitivity to infections.
Regarding the diagnostic relevance of IgE‐ and IgG/IgG4 reactions it should be considered that immunological mechanisms and related symptoms can be different in inhalation allergies and food intolerances. In particular a non‐IgE mediated reaction is known for food intolerances for a long time.
Food Intolerance Tests » For a Better Quality of Life «
Relevance of the detection of specific IgG / IgG4 Immunolab antibodies in the diagnosis of food intolerances
Page 2
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected] Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
PRIN-FI_background_eng_v1
The critics of IgG/IgG4 tests, mainly allergologists and their professional associations, persistently complain about the lack of scientific studies, without presenting concrete own data though. Indeed numerous articles have been published during recent years which support the relevance of the IgG/IgG4 antibodies’ detection for the diagnosis of food intolerances. The conclusion of some of these publications are presented as follows:
A Norwegian group postulated an important immunopathological role of specific IgG4 antibodies in patients with a delayed non‐IgE mediated cow’s milk allergy. This patient group showed significantly higher concentrations of ß‐lactoglobulin (ß‐LG)‐ specific IgG4 antibodies compared to cow’s milk tolerized patients. (2)
A food intolerance reflected by IgG antibodies can be associated with an increased risk of arteriosclerosis and low grade inflammation (elevated CRP levels) in obese juveniles. (3)
Other publications support the high significance of IgG4 tests to determine and monitor an elimination diet in patients with an irritable bowel syndrome. Moreover, the investigations of Zar point to a positive impact on psychic symptoms, based on IgG4 tests.
(4‐7)
Bernardi has found highly significant differences of IgG4 titers in patients with food intolerance to 19 food antigens compared to asymptomatic healthy subjects. The obtained sensitivity and specificity were 81% and 87%, respectively. Based on the described test results exclusion diets were performed leading to a significant improvement of the symptoms in 78.5% of all cases after 2 months only. An IgG4 control testing after this time period proved a manifest reduction of the previously elevated IgG4 titers.
(8)
Even in migraine patients symptoms could be improved by exclusion diets that were based on IgG results. (9)
A less recent publication describes the detection of high IgG4 concentrations in patients with confirmed sensitization to soy beans. In contrast low reactivity was found for IgE and IgG. (10)
In another investigation of patients showing asthma symptoms after contact with soy dust authors found that both specific IgE and IgG4 antibody levels to soy were increased. They postulated that the IgG4 increase is part of the pathological asthma process. (11)
Food Intolerance Tests » For a Better Quality of Life «
Relevance of the detection of specific IgG / IgG4 Immunolab antibodies in the diagnosis of food intolerances
Page 3
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected] Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
PRIN-FI_background_eng_v1
References
1. Snapper et al. (1999) in Fundamental Immunology, ed. Paul, W. E. (Lippincott, Philadelphia), pp. 831–861.
2. Sletten et al. (2006). Changes in humoral responses to beta‐lactoglobulin in tolerant patients suggest a particular role for IgG4 in delayed, non‐IgE‐mediated cow's milk allergy. Pediatr. Allergy Immunol. 17, 435‐443.
3. Wilders‐Truschnig, M. et al. (2008). IgG Antibodies against Food Antigens are Correlated with Inflammation and Intima Media Thickness in Obese Juveniles. Exp Clin Endocrinol Diabetes 116(4):241‐5.
4. Atkinson et al. (2004). Food elimination based on IgG antibodies in irritable bowel syndrome, a randomised controlled trial. Gut 53:1459–1464.
5. Zar et al. (2005). Food‐specific IgG4 antibody‐guided exclusion diet improves symptoms and rectal compliance in irritable bowel syndrome. Scand J. Gastroenetrol. 40, 800‐807.
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Infectious Disease ELISA » We Protect Your Health «
Immunolab
Immunolab GmbH, an acknowledged developer and manufacturer of high‐quality immunodiagnostic and analytical reagents, provides you with a wide range of enzyme immunoassays for the detection of infectious disease parameters. Our product line is suitable for routine applications as well as research purposes.
The Immunolab Infectious Disease Tests are microtiter plate based enzyme immunoassays (ELISA) for the qualitatitive and quantitative determination of IgA, IgG and IgM antibodies to multiple infectious agents in human sera and plasma. Further applications in other body fluids are possible.
Each kit includes all ingredients required to perform the assay. One kit provides 96 tests. All reagents are ready‐to‐use. Assay Performance Scheme:
Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de PRIN-IN_general info_eng_v2
Features and Benefits
Broad Panel including TORCH
IgG, IgA and IgM detection for most parameters
Short Assay Time
Easy Handling by:
o Uniform Test Format
o Ready‐to‐use and interchangeable Reagents
o Breakable Strips
o Standardized Performance (identical volumes, dilution)
o Identical Incubation Times for all parameters
o Incubation at ambient temperature
Wide Reference Range by 4 point calibration
Qualitatitive (using cut‐off) and/or Quantitative (using calibrators) Test Results
Quantitative Result with just one sample dilution
High Stability, minimum shelf life 12 months
Registered in the European Union according to IVDD 98/79/EC
Immunolab Infectious Disease ELISA
Adenovirus (IgA, IgG, IgM) Aspergillus fumigatus (IgA, IgG, IgM) Bordetella pertussis (IgA, IgG, IgM) Bordetella pertussis Toxin (IgG) Bordetella FHA (IgG) Borrelia burgdorferi (IgG, IgM) Brucella abortus (IgA, IgG, IgM) Candida albicans (IgA, IgG, IgM) Cytomegaly Virus (IgG, IgM) Diphtheria (IgG) Echinococcus granulosus (IgG) Epstein‐Barr‐Virus EA (IgA, IgG, IgM) Epstein‐Barr‐Virus EBNA (IgA, IgG, IgM) Epstein‐Barr‐Virus VCA (IgA, IgG, IgM) Helicobacter pylori (IgA, IgG, IgM) Herpes simplex (HSV) 1 (IgA, IgG, IgM) Herpes simplex (HSV) 2 (IgA, IgG, IgM) Herpes simplex (HSV) 1/2 (IgA, IgG, IgM) Influenza A (IgA, IgG, IgM) Influenza B (IgA, IgG, IgM) Measles (IgA, IgG, IgM) Mumps (IgA, IgG, IgM) Mycobact. tuberculosis (IgA, IgG, IgM) Mycoplasma pneumoniae (IgA, IgG, IgM) Parainfluenza 1/2/3 (IgA, IgG, IgM) Resp. Syn. Virus (RSV) (IgA, IgG, IgM) Poliomyelitis (IgG) Rubella (IgG, IgM) TBE Virus (FSME) (IgG, IgM) Tetanus (IgG) Toxoplasma (IgG, IgM) Varicella zoster (IgA, IgG, IgM) Yersinia enterocolitica (IgA, IgG, IgM)
Probe (1:100)
Antigen beschichtete Mikrotiter-Kavität
E
Spezifische Antikörper binden an das Antigen
Sekundärantikörper (Konjugat) bindet an die Fc-Region des spezifischen Antikörpers
60 Min, RT
HRP (E) Enzymaktivität wandelt das farblose- in blaues TMB um.
Messung Konjugat (HRP)
30 Min, RT
Substrat (TMB)
20 min, RT
StopplösungE E E E E
450 nm
OD
Testresultate
Konz.
Stopplösung beendet die Enzymreaktion und führt zu einem gelben Farbumschlag
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected]
Infectious Disease ELISA – TORCH » We Protect Your Health « Immunolab
Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected] Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de
PRIN-IN_torch_eng_v1
TORCH is an acronym for a group of infectious diseases showing an increased prevalence to cause illness in pregnant women and birth defects in their newborns. The TORCH panel consists of the following tests:
Toxoplasma gondii, Rubella virus, Cytomegalovirus (CMV) and Herpes simplex virus type 1 + 2.
Immunolab GmbH, an acknowledged developer and manufacturer of high‐quality immunodiagnostic and analytical reagents provides you with the complete TORCH panel. Our highly sensitive and specific Toxoplasma, Rubella, Cytomegaly and Herpes simplex kits are in compliance with the European in‐vitro diagnostic directive (IVDD) 98/79/EC and as such are CE marked. The Immunolab TORCH tests are microtiter plate based enzyme immunoassays (ELISA) for the qualitatitive and quantitative determination of IgG, IgM and IgA (only HSV) antibodies to Toxoplasma gondii, Rubella, Cytomegalovirus (CMV) and Herpes simplex virus in human sera and plasma.
Each kit includes all components required to perform the assay. One kit provides 96 tests. All reagents are ready‐to‐use. Assay Performance Scheme:
Features and Benefits
• Short Assay Time
• Easy Handling by:
o Uniform Test Format
o Ready‐to‐use and interchangeable Reagents
o Breakable Strips
o Standardized Performance (identical volumes, incubation times, dilutions)
o Incubation at ambient temperature
• High Stability, minimum shelf life 12 months
• Test results are qualitatitive (using cut‐off) and/or quantitative (using calibrators)
• Quantitative Result with just one sample dilution
• Reference curve calibrated against International WHO Reference Preparations: o Rubella IgG, 1st Intl. Standard RUBI‐1‐94. o Toxoplasma IgG, standard TOXM (3rd Intl. Standard)
• Product Registration acc. to the Europ. in vitro diagnostic directive IVDD 98/79/EC
Immunolab Infectious Disease ELISA Adenovirus (IgA, IgG, IgM) Aspergillus fumigatus (IgA, IgG, IgM) Bordetella pertussis (IgA, IgG, IgM) Borrelia burgdorferi (IgG, IgM) Brucella abortus (IgA, IgG, IgM) Candida albicans (IgA, IgG, IgM) *Cytomegaly Virus (IgG, IgM) Diphtheria (IgG) Echinococcus granulosus (IgG) Epstein‐Barr Virus EA (IgA, IgG, IgM) Epstein‐Barr Virus EBNA (IgA, IgG, IgM) Epstein‐Barr Virus VCA (IgA, IgG, IgM) Helicobacter pylori (IgA, IgG, IgM) *Herpes simplex 1, 2 and 1/2 (IgA, IgG, IgM) Influenza A and B (IgA, IgG, IgM) Measles (IgA, IgG, IgM) Mumps (IgA, IgG, IgM) Mycobact. tuberculosis (IgA, IgG, IgM) Mycoplasma pneumoniae (IgA, IgG, IgM) Parainfluenza 1/2/3 (IgA, IgG, IgM) Resp. Syn. Virus (RSV) (IgA, IgG, IgM) Poliomyelitis (IgG) *Rubella (IgG, IgM) TBE Virus (FSME) (IgG, IgM) Tetanus (IgG) *Toxoplasma gondii (IgG, IgM) Varicella zoster (IgA, IgG, IgM) Yersinia enterocolitica (IgA, IgG, IgM)
*TORCH Kits marked in red and bold letters
Sample (1:100)
antigen coated microtiter well
E
specific antibody binds to immobilized antigen
secondary antibody (conjugate) binds to fc of specific antibody
60 min, RT
HRP enzyme activity converts colorless TMB into blue TMB
Measurement Conjugate (HRP)
30 min, RT
Substrate (TMB)
20 min, RT
Stop Sol.E E E E E
450 nm
Test Results stop sol. terminates color development and changes color to yellow
OD
Conc.