FabRICATOR -HPLC » FabRICATOR... · The performance of the FabRICATOR-HPLC column at 37°C is...

2018/10/19 Genovis » FabRICATOR-HPLC

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Genovis / Products / IgG Proteases / FabRICATOR / FabRICATOR-HPLC

FabRICATOR®-HPLCFabRICATOR-HPLC is an HPLC column for fast on-column digestion of monoclonalantibodies.

The cysteine protease FabRICATOR (IdeS) immobilized on the column delivers rapid andconsistent subunit generation, perfect for automated online analysis in a middle-level LC/MSworkflow.

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“FABRICATOR®-HPLC – AUTOMATED ONLINE SUBUNIT GENERATION”














Detailed Information

The FabRICATOR-HPLC column with immobilized FabRICATOR (IdeS) enzyme digests IgGrapidly and specifically just below the hinge, generating F(ab’)2 and Fc fragments without anyrisk of over-digestion. The FabRICATOR-HPLC column is designed as an analytical tool forrapid on-column digestion in middle-level workflows for characterization of antibodies andFc-fusion proteins. After digestion, the fragments can be analysed using reversed phaseHPLC, mass spectrometry or other analytical method. Automation of antibody digestion andsubunit generation enables applications in online monitoring of bioreactors, for example aspart of a 2D-LC-MS setup, coupling the reactor directly the MS. This automated approach toantibody subunit analysis reduces operator time, sample handling and increases thethroughput. Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message Leave a message

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In-house performance testing* of the FabRICATOR-HPLC column highlights the stability andreproducibility, for complete data see here. The column delivered consistent digestion for 14days at 37°C. More than 450 injections of mAb were made during testing and no carry-overwas observed. Reproducible Fc glycan profile analysis was obtained showing the column tobe suitable for online process monitoring. Modified Fc glycans on a therapeutic mAb werealso detected using FabRICATOR-HPLC fragmentation followed by LC-MS.

Learn more about the applications of FabRICATOR-HPLC

* Tested with trastuzumab in PBS, injections every 40 minutes, at a flow rate of 25 µl/min, samples

collected, reduced and analyzed by RP-HPLC.

Storage

Store the column at +4-8°C in 10 mM sodium phosphate, 140 mM NaCl, pH 7.4 when not inuse. Store the column with the endcaps in place, carefully sealed to prevent drying. For long-term storage of the column 0.02% (w/v) sodium azide can be added as apreservative.

Performance The performance of the FabRICATOR-HPLC column at 37°C is typically >200 injections witha digestion efficiency of >95% for 10 µg human IgG1 (1 mg/ml) sample in PBS, pH 7.4.

Product Overview

Column dimensions: 2.1 mmD x 50 mmL Support resin: POROS™

A0-FRC-050

FabRICATOR-HPLC

€1,599.00

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Typical flow rate: 0.025-0.05 mL/min Maximum Pressure: 100 bar Operating pH: 6.5 - 8.0 Operating temperature: 37°C Storage conditions: +4°-8°C

Number of days of continuous operation: >10 * Injections per column: >200 *

Start material: Human IgG1-4, Fc-fusion proteins

* Depending on specific application

Download Poster

Download CASSS MS Poster”Rapid On-column Monoclonal Antibody Subunit Generation for Automated Glycan Analysis”

Applications

Human IgG1-4, IgG from monkey, rat, rabbit and sheep

Over 95% digestion of 10 ug IgG in less than 5 minutes

FabRICATOR-HPLC Column for automated subunit analysis


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Find all applications here

Key Characteristics

How to Order

For details on ordering please visit How to order. Click add to cart above to buy online.

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Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulkquantities or licensing opportunities.

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Instructions

Product Specifications

Safety Data Sheet

Automated glycan profiling•

Bioreactor monitoring•

2D-LC-MS characterization workflows•

Follow ongoing reactions•

FabRICATOR-HPLC column enables automated subunit generation•

Complete digestion of 10 ug IgG in less than 5 minutes•

No carry-over observed in testing•

Reduce manual sample handling errors•

Increase throughput of subunit analysis•

FabRICATOR-HPLC•

FabRICATOR-HPLC•



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FAQ and Support

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Patent and Disclaimer

FabRICATOR®

All rights reserved. Aspects of FabRICATOR® technology are encompassed by pending patent

applications in the name of Genovis AB.

The trademark FabRICATOR® is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

©2018 Genovis AB.

POROS™

This product is provided under an intellectual property license from Life Technologies Corporation. The

transfer of this product is conditioned on the buyer using the purchased product solely in research or for

analytical testing during manufacturing, all conducted by the buyer, excluding contract research or any fee

for service research, and the buyer must not (1) use this product or its components for (a) diagnostic,

therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for

FabRICATOR-HPLC•

What is the FabRICATOR-HPLC?

Can I use FabRICATOR-HPLC in a 2D-LC setting?

How many samples can I run through a column?


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compensation on a per-test basis; or (c) sell or transfer this products or its components for resale, whether

or not resold for use in research. For information on purchasing a license to this product for purposes

other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA

92008 USA or [email protected].

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FabRICATOR -HPLC » FabRICATOR... · The performance of the FabRICATOR-HPLC column at 37°C is...

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