f~,5- · of 1965. A graduate seminar on Blood Brain Barrier was given by Dr. Thompson in the fall...

ANNUAL REPORT - DEPARTMENT OF PHYSIOLOGYUNIVERSITY OF KANSAS MEDICAL CENTER

July I, 1964 -June 30, 1965

ACADEMIC STAFFE. B. Brown, Jr., Ph. D., Professor and ChairmanAlan M. Thompson, Ph. D. Associate ProfessorNorman Loofbourrow, Ph. D., Associate ProfessorLawrence Sullivan, Ph. D., Assistant ProfessorDonald Johnson, Ph. D., Assistant Professor (Joint appointment in

Obstetrics and Gynecology. )Kenneth Goetz, Ph. D., InstructorJohn Trank, Ph. D., Assistant ProfessorA. Stark Wolkoff, M. D., Assistant Professor (Joint appointment in

Obstetrics and Gynecology. )

POSTDOCTORAL FELLOWS

Virginia Tucker, M. D., Department of Pediatrics (returned toDepartment of Pediatrics September 1964. )

James Evans, M. D., Division of Anesthesiology (returned toAnesthesiology October 1964.)

Benjamin Razon, M. D., AID Fellow, Far Eastern University(returned to Philippines September 1964.)

Hiroshi Aoki, M. D., Surgical Fellow from Japan (returned toJapan August 1964).

Joseph McMullen, M. D., Department of SurgeryDarren Nelson, Ph. D., Ob-Gyn Fellow with Dr. JohnsonTomi-O Hishida, D. Sc., Nagoya University, Fellow with Dr. Johnson'Woo Gyeum Kim, M. D., (IAEA) International Atomic Energy Agency

Fellowship, Seoul National University, Korea.

GRADUATE STUDENTS

Richard L. Clancy - / f~,5-Robert VI. Hamilton, Jr. If 6 t-(~ '21Robert Thurber - /76f)"Dale Mayhew -Lilly Kung-Gary Slick "-FranklinR. Darrow -Ray CookLarry WellingPaul Hensleigh

-~~ , ,

Annual Report-Dept. Physio!.7/1/64 - 6/30/65

TEACHING

The medical school course for freshmen was changed so that Neuro-anatomy was taught in the fall quarter. Physiology and Biochemistrywere still taught during the winter and spring quarters, except for thelast two weeks of the spring quarter, at which time the integrated cour sein Endocrinology was presented.

Current course offerings of the department are the same as for lastyear, except for the addition of graduate course #327, PhysiologicalInstrumentation (3 credits) which will be taught by Dr. John W. Trankduring the winter quarter of even numbered years.

Two graduate courses and two graduate seminars were presented. Inthe winter quarter 1964, Dr. Johnson presented his course #325 onBiology of Sex (3 c;redits); Dr. Brown's graduate course #320 on Acid-Base and Electrolyte Meta1;>olism w"as taught in the spring quarterof 1965. A graduate seminar on Blood Brain Barrier was given by Dr.Thompson in the fall of 1964, and Dr. Sullivan presented his graduateseminar course #351 on the Role of the Kidney in Acid-Base Balanceduring the spring quarter of 1965.

In addition to the senior staff members, Mr. Richard Clancy (Instructor)and Dr. Virginia Tucker were responsible for a Conference Section ofone group of students in the medical school Physiology course. Gradu-ate students Clancy, Mayhew, Darrow, Slick, and Kung, and Dr. Kimalso assisted with teaching the medical school Physiology laboratorycourse.

During the summer of 1964 the integrated course in Basic HumanBiology for Nurses was again taught by the combined Departments ofBiochemistry, Anatomy, and Physiology. Drs. Sullivan and Thompsonwere the senior staff members from this department participating inthis course. Medical students Luther Frye, Wayne Figgs, and JackReida assisted in the course.

The Surgery-Physiology Conference was again held each Wednesdayafternoon at 4 P. M. during the academic year.

Three graduate students completed work for the Ph. D. degree in thisdepartment during the past academic year. Robert W. Hamiltonreceived the Ph. D. degree from the University of Minnesota GraduateSchool in December 1964. He is now employed as a Physiologist withThe Union Carbide Corporation, Linde Division, Tonawanda, New York.

Annual Report-Dept. Physiol.7/1/64-6/30/65

RichardL. Clancy and Robert Thurber both received Ph. D. degreesfrom the University of Kansas in the June commencement, 1965. Dr.Thurber has accepted a position as Instructor in the Department ofPhysiology in the Medical College of Virginia at Richmond, Virginia.Dr. Clancy will work with Dr. Stanley Sarnoff as a postdoctoralfellow for the next two year s in the Cardiac Research Unit, NationalInstitutes of Health, Bethesda, Maryland.

The department was strengthened by the addition of Drs. John Trankand Stark Wolkoff to our Senior Staff during the year. Dr. Trank,appointed as Assistant Professor, came to this department fromMontreal. Canada, where he had been associated with both the Depart-ments ofSurgery and Physiology at McGill University Medical School.In addition to his doctorate degree in Physiology, Dr. Trank holds aBachelors degree in Electrical Engineering and a Masters degree inBiophysics. He brings much needed strength in the area of Biophysicsto this department and to the Medical Center. Dr. Trank transferreda research grant supported by the Life Insurance Medical ResearchFund from McGill Univer sity to this Institution.

Dr. Stark Wolkoff began an appointment as Associate Professor ofObstetrics and Gynecology and Assistant Professor of Physiology on

January 1, 1965. Dr. Wolkoff also transferred a research grant sup-ported by the US PHS from the University of North Carolina. Dr.Wolkoff's research deals with fetal physiology, which he studies in

sheep.

July I, 1964, was the activation date for a Training Grant in Physiologysupported by the USPHS. This grant makes it possible for us to sup-port an additional six graduate students in Physiology. The number ofapplications for admission to graduate work in this department hassteadily increased over the past three years, and it appears likelythat this increase will continue.

Medical students who engaged in research projects in this department

during the summer of 1964 were Terry Sutton, John Hunkeler, R. (Clio)Robertson, Tom Bauer, Dick Worley, Dave Reichert, Bill Hawley,Charles Hayes, Jerry Tulecke, Cleve Howard, Alan Moorhead, RalphKauffman, Rodney Thompson, and Barbara Cole.

The Kaw Valley Heart Association, through its Summer Search Talentprogram, sponsored Bruce Wolhuter and Roy Meals for researchtraining in this department.


VISITORS AND OUTSIDE SPEAKERS:

The following visiting professors presented lectures during the year:

10/29/64 Dr. Min Haing Cho, Dept. of Pharmacology and Anesthesiology,State University of New York, Upstate Medical Center, Syracuse,New York, "Function of Histamine. If

2/3/65

2/22/65

3/29/65

3/30/65

4/19/65

3/15/65

3/22/65

4/26/65

5/3/65

Dr. David Schafer spoke informally about his work on cholera.Dr. Schafer is a Fulbright Lecturer in Biophysics at the Univ.of Calcutta and a member of the Johns Hopkins team workingon cholera there.

Dr. Charles L. Johnston, Jr., Assistant Professor of Physiology,University of North Carolina, Chapel Hill, spoke on liSome Studiesof Villikinin. II

Lerner B. Hinshaw, Ph. D., Physiologist. Civil AeromedicalResearch Institute. Norman, Oklahoma, talked on "Endotoxin

Shock: Mechanisms and Therapy. II

Dr. James W. Bawdin, Assistant Dean for Research, Univ. of

North Carolina School of Dentistry, talked with our group on"Calcium and fluoride exchange across the placenta. II

Dr. Hebbel Hoff, M. D., Ph. D., Chairman, Dept. Physiology,Baylor University Medical School, lectured on "ClaudeBernard and the Experimental Methodf~

Dr. D. R. Justesen, Chief Research Psychologist, VA Hospital,K. C., Mo. "Electroconvulsive shock and correlates with shortterm memory. I)

Dr. Paul Johnstone of UMKC spoke on "Studies on the Physio-logical anatomy of the embryonic heart: Observations on thedevelopment of the EKG in the embryonic chick heart. II

Dr. James O. Elam, Professor and Chairman, Department ofAnesthesiology, University of Missouri at Kansas City GeneralHospital, Kansas City, Missouri. "Regulation of respirationduring halothane anesthesia in man. fi

Dr. Charles L. Sheridan, Univ. of Alberta, Calgary, Canada.f'Current developments in the split brain approach to theproblem of memory localization. II


5/10/65 Dr. Donald R. Boyer, Dept. of Biology, Washburn Univ. ofTopeka, Topeka, Kansas. "Effects of hypoxia on respiratoryand cardiac physiology in reptiles. 11

6/14/65 Dr. Floyd Rector, Dept. of Medicine, Southwestern UniversityMedical School, Dallas, Texas, spoke on IIMechanism ofRenal Bicarbonate Reabsorption. ",

ACTIVITIES OF STAFF:

Dr. Brown:Editor:

Proc. Soc. Exp. Biology and Medicine.Modern Medicine.

Member NIH Physiology Fellowship Panel.

Invited speaker at The New York Academy of Sciences Symposiumon "Current Concepts of Acid-Base Measurements", Nov. 23, 1964.

Spoke at the KUMC Research Society Meeting in Wahl Hall East,February 5, 1965, on l'Intracellular pH and Tissue Buffering. "

Took part in the Postgraduate Department's program by lecturingon "Pathophysiology of Edema" at St. Joseph, Missouri, onNovember 17, 1964.

Spoke at Washburn University of Topeka, Topeka, Kansas, March10, 1965, on "Intracellular pH and Potassium Metabolism. "

Dr. Sullivan:

Seminar speaker at Baker University, Biology Department,Baldwin, Kansas, March 9, 1965.

Gave lecture at Medicine Grand Rounds in the fall of 1964.

Attended F ASEB meetings in spring of 1965.

Attended Biophysical Society meetings in San Francisco inFebruary 1965.

Member of KUMC Research Committee.


t

Dr. A. M. Thompson:Review Editor for papers submitted to "Science. II

Member of KUMC Pdmissions Committee.

Robert Thurber, graduate student, presented a paper at thespring meeting 1965 of the APS covering joint work done byMr. Thurber and Dr. Thompson.

Addressed the Science Colloquium at Park College, Parkville,Missouri, in March 1965.

Gave lecture fur Neurology Course on "Cerebral Blood Flow. II

Dr. G. N. Loofbourrow:

The Neurology Section of the Department of Medicine, Divisionof Neurosurgery and the Basic Science Departments of the KUMCpresented a series of lectures on basic topics in neurologicalsciences. Dr. Loofbourrow was responsible for the last thirdof this lecture program.

Dr. Kenneth Goetz:

Presented paper atAmerican Heart Meetings, October 1964.

Attended spring meetings of F ASEB

Lectured to Staff at Topeka State Hospital, IvIay 11, 1965.

Dr. James A. Evans:

Paper on Methoxyflurane Effects on Hepatic Blood Flow wonsecond prize in Resident Section at New York State Society ofAnesthesiologists, December 16, 1964.

Dr. John W. Tranle

Dr. Trank is a new member of our staff since August 1, 1964.He has been busy setting up his laboratory and teaching.Lectured to Science Classes at Broadmore Junior High School.Overland Park, Kansas.Lectured at William Jewel College to the Beta Beta BetaBiology Society on April 27, 1965.Lectured for Neurology Course on Biological Transducers.

Dr. Wolkoff:

From May 3 to 28, Dr. Wolkoff was at the Institute of NuclearStudies in Oak Ridge, Tennessee, taking a course in Radio-isotope Technique s.

Annual Report-Dept. Physiol.7/1/64 - 6/30/65

RESEARCH

Research grants which were active during the year were the following:

Ventilatory responses to chemical stimuli in arterial blood. Air ForceContract #AF 41-(657)-400, USAF School of Aerospace Medicine, BrooksAir Force Base, Texas. Principal Investigator: E. B. Brown, Jr.August 1, 1961 to July 31, 1964. $27,400. Expired at end of grant year.

Potassium metabolism and acid-base balance. United States Public

Health Service #5649. Principal Investigator: E. B. Brown, Jr.June 1, 1964 - May 31, 1965, $16,560. June I, 1965 - May 31, 1966, $17,160.

A study of cardiovascular regulatory mechanisms. USPHS #HE 07632 (PHY).Principal Investigator: E. B. Brown, Jr. Co-Principal Investigator:C. Frederick Kittle. Dec. 1, 1963 - Aug. 31, 1964, $13, 220. Expiredat end of grant year.

Tissue CO2 chemical forms and exchange rate. USPHS #GM-10429.Principal Investigator: Alan M. Thompson. Sept.!, 1963 - Aug. 31, 1964,$17,250. Sept. 1, 1964 - Aug. 31, 1965, $14,400.

Control of collecting duct secretion of hydrogen and potassium. NationalScience Foundation #GB-655. Principal Investigator: Lawrence P Sullivan.Jan. I, 1963 - Dec. 31, 1964, $16,900. Jan. 1, 1965 - Dec. 31, 1965, $16,900.

Hydrogen Ion retention in diabetic acidosis and ketosis. USPHS #AM-06570-02. Principal Investigator: Virginia Tucker; Co-Principal Investigator:E. B. Brown, Jr. Sept. 1, 1963 - Aug. 31, 1964, $12,328. Sept. 1, 1964 -Aug. 31, 1965, $12,024.

Regulatory Mechanisms of blood flow. USPHS #HE-08609-01Al. PrincipalInvestigator: C. F. Kittle. Co-Principal Investigator: E. B. Brown, Jr.March 1, 1965 - Feb. 28, 1966, $18,391.

Right Atrial pressure and heart rate. USPHS #lROIHE 08803-01 (PHY).Principal Investigator: Kenneth Goetz. June 1, 1964 - May 31, 1965,$14,417. June 1, 1965 - May 31, 1966, $13,320.

Motor Effects of the arrest reaction. USPHS #MH 08908-01 EP lRO!.

Principal Investigator: G. N. Loofbourrow. June 1, 1964 - May 31, 1965,$15,582. June 1, 1965 - May 31, 1965 $

Experimental Production of pulmonary hypertension. PHS #HE-04392-04.Principal Investigator: C. Frederick Kittle. Oct. 1, 1963 - Sept. 30,1964, $8,592.

Annual Report-Dept. Physiol.7/1/64 - 6/30/65

Studies on the Maternal-Fetal Relationship in Sheep. United StatesPublic Health Service #HD-00556-03. Principal Investigator: A. StarkWolkoff. Jan. 1965 - Dec. 31, 1965, $11,000.

PHS Training Grant. Program Director: E. B. Brown, Jr.1137-01Al. July 1, 1964 - June 30, 1965, $30,000.

# 1 T 1 GM

General Research Support Grant. Allocated to Department of Physiology.Inst. Grant #589-1240229. Jan. 1, 1964 - Dec. 31, 1964, $3,000;Jan. 1, 1965 - Dec. 31, 1965, $3,000. Inst. Grant #09589045.

The hepato toxicity of anesthetic agents. Institutional grant #589-124-4100517. Principal Investigator: Alan M. Thompson. Jan. 1, 1964 -Dec. 31, 1964, $1330.

The Effect of changes in right atrial pressure upon the heart rate.Institutional Grant #589-124-4100189. Principal Investigator: KennethGoetz. Jan. 1, 1964 - Dec. 31, 1964, $2,000.

Control of collecting duct secretion of potassium and hydrogen. Inst.Grant #589-124-4100510. Principal Investigator: L. P. Sullivan.Mar. 15, 1964 - Dec. 31, 1964, $3,500.

In vivo blood buffer curves. Kansas Heart Association #63RlZ18--Principal Investigator: E. B. Brown, Jr. July 1, 1964 - June 30,1965, $3,250.

Right atrial pressure and heart rate. Kansas Heart Association #63R1321.Principal Investigator: K. L. Goetz. July 1, 1964 - June 30, 1965, $2200.

Life Insurance Medical Research Fund. KU Endowment Association#64R1237. Principal Investigator: John Trank. July 7, 1964 -$9,000.

Distribution of sucrose and plasma protein. American Medical Asso-ciation. Med. Ctr. #115 -E. Principal Investigator: Alan M. Thompson.$1,000. (can be used until money is all spent)

Fellowship Award, Public Health #1-FI-GM-20, 339-01. PrincipalInvestigator: Alan M. Thompson, Fellow: Robert E. Thurber.June I, 1963 - May 31, 1964, $7,000.

AnnualReport-Dept. Physiol.7/1/ 64 - 6/30/65

Fellowship award, Public Health #5-FI-GM-13, 199-04. PrincipalInvestigator: E. B. Brown, Jr. Fellow: Richard L. Clancy. Jan. 15,1964 - Jan. 14, 1965, $5,700. (can be used until money is all spent)

Fellowship Award, Public Health #1-F2-GM-2l, 994-01. PrincipalInvestigator: E. L. Fredrickson and A. M. Thompson. Fellow: James A.Evans. Oct. 1, 1963 -Sept. 30,1964, $8,500.

PUBLICATIONS

Clancy, Richard L., and E. B. Brown, Jr. Potassium movement incardiac muscle during changes in intracellular hydrogen ion. Physiologist7:105, 1964.

Brown, E. B., Jr., and Richard L. Clancy. Further observations onin vivo CO 2 Buffer Curves of Blood. Physiologist 7: 96, 1964.

Hamilton, R. 'vV., Jr. and E. B. Brown, Jr. Interaction of chemicalstimuli in the control of breathing. Fed. Proc. 24: 27 2, 1965.

Kittle, C. F., H. Aoki, and E. B. Brown, Jr. The role of pH and CO2in the distribution of blood flow. Surgery, January, 1965.

Brown, E. B., Jr. and R. L. Clancy.Curves. J. Appl. Physiol. (In press)

In vivo and In Vitro Blood Buffer

Hamilton, R. V!., Jr., M. S. Continuous sampling of arterial blood ofunanesthetized Animals. J. Appl. Physiol. 20:555, 1965.

Hamilton, R. Vi., Jr., M.S. ,R.F. Hustead, and L. F. Peltier. FatEmbolism: The effect of particulate embolism on lung surfactant. Surgery56:53, 1964.

Samson, F. E., Jr., G.N. Loofbourrow, D.S. Rube, and J. F. Vrhiting.

Utilizing television for laboratory experiments in physiology. The J. Med.Education 39:780, 1964.

Henning, Charles. An implantable pacemaker with automatic rate regulation.J. Ks. Med. Soc., Nov., 1964, pp. 560-562 Vol LXV, No. XII. (StudentThesis-written while a fourth year student at the Univ. of Ks. Sch. of Med. )

Haning, Quentin C., and A. M. Thompson. A comparative study of tissueCO2 in vertebrates. Compo Biochem. and Physiol. 15:17-26, 1965.

Annual Report - Dept. Physio!.-7/1/64 - 6/30/64

Go~tz. Kenneth L. Effect ofincreasedpressurewithin a right heartcu~-de-sac upon heart rate in dogs. Am. J. Physiol. (Inpress)

Goet~, Kenneth L. Effect of pressure changes within the right heart uponthe heart rate in dogs (Abstract) Circulation 30:Supp. 3. 85, 1964.

How~rd. C. W., K. L. Goetz, G. L. Slick a9d D. C. Johl}son. Alteration,of coronary blood flow with changes in pressurewithin the right heart.Fed. Proc. 24:528, 1965.

HamU~on. R. VI., Jr. and A. Schoolman. Acid-base changes in catsfollowing exper~mental epileptic seizures. Neurology. 15:444-448, 1965.

Kuenzig. M.D., R. W. Hamilton, Jr., and L. F. Peltier. Surface pro-pe:rties of dipalmitoyl lecithin. J. Appl. Physio!. (In press)

Hamilt"n, R. Vi., Jr., CCI,rbon dioxide. oxygen and acidity: The inter-action and independent effects on respiration of these factors in thearte1'ia~ blood. USAF School ofAerospace M~d. Report 64-94, BrooksAir Force Base, Texas. (Dec. 1964)

Thurber, Robert E. and A~an M. Thompson. Stationary state ion distri-butions across human erythrocyte membra~es. (Abstt'act) Fed. Proc.24:464, 1965.

Johnson, D. C. Effect of hypothalami extract on hypop~yseal LH inimmatq.re male rats. Proc. Soc. Exper. BioI. and Med.: 117:160-163, 1964.

Yasuda, M., D. C. Johnson. Effects of exogenous androgen and gona-dotropins on the testes and hypophysial follicle-stimulating hormone contentof the immature male rat. Endocrinology 76: 1033-1039. 1965.

Johnson, p. G. Estimation of serum gonadotropin by use of parabiotic rats.Anat. Ree. 151: 366. 1965. (Abstract of pape:r presented at the 78th AnnualSession qf the American Association of Anatomists,)

SullivC!.n, L. P. Ammonium excretion during stopped flow: An ammoniumcoUntercurrent hypothesis. Am. J. Physio!. (In press)

f~,5- · of 1965. A graduate seminar on Blood Brain Barrier was given by Dr. Thompson in the fall...

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