Exercise 3: Simple Staining...
Transcript of Exercise 3: Simple Staining...
![Page 1: Exercise 3: Simple Staining Techniquelpc1.clpccd.cc.ca.us/LPC/Zingg/Micro/PrelabPP_FS11/Ex3...Exercise 3: Simple Staining Technique Remember the two important rules: 2. Never use the](https://reader034.fdocuments.in/reader034/viewer/2022052014/602b7a73dbfbf3499d7b95b5/html5/thumbnails/1.jpg)
Exercise 3:
Simple Staining Technique
Remember the two important rules:
2. Never use the coarse adjustment with the 40x and 100x objectives.
![Page 2: Exercise 3: Simple Staining Techniquelpc1.clpccd.cc.ca.us/LPC/Zingg/Micro/PrelabPP_FS11/Ex3...Exercise 3: Simple Staining Technique Remember the two important rules: 2. Never use the](https://reader034.fdocuments.in/reader034/viewer/2022052014/602b7a73dbfbf3499d7b95b5/html5/thumbnails/2.jpg)
Objectives
• Explain the value of staining
microorganisms
• Prepare a specimen slide including air-
drying and heat-fixing
• Explain the principle of a simple stain and
recognize the differences between eu- and
prokaryotic cells
• Identify the most common shapes of
bacteria in your cheek cell smear
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Lab Bench Organization
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![Page 5: Exercise 3: Simple Staining Techniquelpc1.clpccd.cc.ca.us/LPC/Zingg/Micro/PrelabPP_FS11/Ex3...Exercise 3: Simple Staining Technique Remember the two important rules: 2. Never use the](https://reader034.fdocuments.in/reader034/viewer/2022052014/602b7a73dbfbf3499d7b95b5/html5/thumbnails/5.jpg)
Prepare a Cheek Cell Smear
Allow smear to air
dry
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Heat fix
![Page 7: Exercise 3: Simple Staining Techniquelpc1.clpccd.cc.ca.us/LPC/Zingg/Micro/PrelabPP_FS11/Ex3...Exercise 3: Simple Staining Technique Remember the two important rules: 2. Never use the](https://reader034.fdocuments.in/reader034/viewer/2022052014/602b7a73dbfbf3499d7b95b5/html5/thumbnails/7.jpg)
Movie Clip on Heat Fixing
http://deimos.apple.com/WebObjects/Core.woa/Browsev2/laspositascollege.edu
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Staining
![Page 9: Exercise 3: Simple Staining Techniquelpc1.clpccd.cc.ca.us/LPC/Zingg/Micro/PrelabPP_FS11/Ex3...Exercise 3: Simple Staining Technique Remember the two important rules: 2. Never use the](https://reader034.fdocuments.in/reader034/viewer/2022052014/602b7a73dbfbf3499d7b95b5/html5/thumbnails/9.jpg)
Blotting
![Page 10: Exercise 3: Simple Staining Techniquelpc1.clpccd.cc.ca.us/LPC/Zingg/Micro/PrelabPP_FS11/Ex3...Exercise 3: Simple Staining Technique Remember the two important rules: 2. Never use the](https://reader034.fdocuments.in/reader034/viewer/2022052014/602b7a73dbfbf3499d7b95b5/html5/thumbnails/10.jpg)
Using the Oil Immersion Lens
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Cheek cell smear
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Remember the Trouble
Shooting Trouble focusing on the object? Check the
following:
• Is the light adjusted properly?
• Was the object in focus under low power?
• Is the oil touching the lens?
• Is the lens dirty?