Examples of Bacterial Identification

7/28/2019 Examples of Bacterial Identification

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Identification of bacteria


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B

iochem

icaltestforbacteria


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Staining


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G

ramsstain

appearance interpret

Purple Gram positive

Pink to red Gram negative

Gram positiveGram negative


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Appearance on bacteriologicalagar


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She

epbloo

dplate

Beta-hemolysisAlpha-hemolysis

Gamma-hemolysis


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Ma

cConkeyagar

colony appearance interpret

1 pink colony Lactose fermenter


3 no growth -

4 colorless colony Non lactose fermenter


8/20Sh

igella-Sa

lmonella

Agar(S

Sagar)

Colorless colony with black center

Pink colony



2 Colorless colony Non Lactose fermenter

3 Colorless colony with black center Non Lactose fermenter

H2S producing colony

Colorless colony


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V.parahaemolyticusand V.choleraon TCBS

TCBS

Green colony

Yellow colony


1 Green colony Non sucrose fermenter

2 Yellow colony Sucrose fermenter


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biochemical test


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O2

O2

O2

T

ripleSu

garIronAgar(TSI)

K/ADelayed reaction

No

fermentation

pH= 7.4

O2

Dextrose

Mixed acid

Dextrose

+ Lactose

Mixed acid

Dextrose

Mixed acid

A/AInitial reaction

A/A

K/N

Non fermenter

Non lactose fermenter

lactose fermenter


12/20T

ripleSu

garIronAgar(TSI)

tube 1 2 3 4

appearance Slant Yellow red red red

Butt Yellow yellow yellow neutral

Gas + + - -

H2S - - + -

interpret Slant/butt, gas,H

2S

A/AG K/AG K/A+ K/N

1 2 3 4

-Fermentation

Yellow ~Acid (A)

Red~Alkaline (K)

No change ~Neutral (N)

-Gas positive G

-H2S positive +


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LysineIron

Agar(LIA)

tube control 1 2 3 4appearance Slant purple purple red purple purple

Butt purple yellow yellow purple purple

H2S - - - +

interpret Slant/butt -/- +/- -/+ -/+

Slant Lysine Deaminase (LDA)

purple = negative

non LDA producerred=positive LDA producer

Butt Lysine Decorboxylase (LDC)

yellow =negative non LDC producerpurple = positive LDA producer


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SIM

tube 1 2 3

sulfur - - +

indole - + +

motile - + +

Sulfur, indole, motile -/-/- -/+/+ +/+/+

1 2 3


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Simmons Citrate agar

1 2

tube 1 2

appearance bacteria No growth growth

agar Not change Blue

interpret - +

- To tests the ability of organisms to utilize citrate as a carbon source

- Sodium citrate is the sole source of carbon


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Tolerance testing


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Bacitrac

insusce

ptibility

test

Clear zone

susceptibility positive

No clear zone resistance negative


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control 1 2

appearance clear clear turbid

interpret - Negative (-) Positive (+)

6.5

%N

aCl

1 2


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Immunological testing


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Anti-serum agglutination test

1 2

appearance Non agglutinate agglutinated

interpret Negative (-) Positive (+)

1 2

Examples of Bacterial Identification

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