Bacterial identification procedure Part 2 : Reading of API system.
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Transcript of Bacterial identification procedure Part 2 : Reading of API system.
Materials avalaible Materials avalaible
Computer with bacterial identification software
API system after incubation
Reagents
API results card
Four complementary tests must be realized.
TDA test : add 1 drop of FeCl³ reagent.
IND test : add 1 drop of Kovacs reagent.
VP test : add 1 drop of -naphtol reagent and 1 drop of KOH reagent.
NR test : in the GLU test cup, add 1 drop of -naphtylamin reagent and 1 drop of sulfanilic acid reagent.
3.1. For each test observe the colors
3.2. With the API datasheet, deduce if the test is positive or negative.
4.2. Report your results + and – on the software cells.Press « enter » on the keyboard to move cell fromcell.
4.1. On the computer, open the bacterial identification software.