Emerging Diversity Within Well-known Heterotrophic Flagellates Groups Revealed by Environmental...

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Institut de Ciències del Mar Ecology and Diversity of Aquatic Protists Advances and Methodologies

Transcript of Emerging Diversity Within Well-known Heterotrophic Flagellates Groups Revealed by Environmental...

Page 1: Emerging Diversity Within Well-known Heterotrophic Flagellates Groups Revealed by Environmental Surveys

Institut de Ciències del Mar

Ecology and Diversity of Aquatic Protists Advances and Methodologies

Page 2: Emerging Diversity Within Well-known Heterotrophic Flagellates Groups Revealed by Environmental Surveys

Ecology and Diversity of Aquatic Protists Advances and Methodologies

34 works based on clone libraries have been published since the first study in 2001

Page 3: Emerging Diversity Within Well-known Heterotrophic Flagellates Groups Revealed by Environmental Surveys

Ecology and Diversity of Aquatic Protists Advances and Methodologies

Organism Taxonomic affiliation*

Cafeteria roembergensis Eukaryota; Chromoalveolata; Stramenopiles; Bicosoecida

Caecitellus parvulus Eukaryota; Chromoalveolata; Stramenopiles; Bicosoecida

Boroka karpovii AKA Pseudobodo tremulans

Eukaryota; Chromoalveolata; Stramenopiles; Bicosoecida

Neobodo designis Eukaryota; Excavata; Kinetoplastea; Metakinetoplastina; Neobodonida

Rhynchomonas nasuta Eukaryota; Excavata; Kinetoplastea; Metakinetoplastina; Neobodonida

Ancyromonas sigmoides Eukaryota; Incertae sedis; Ancyromonas

Amastigomonas debruynei Eukaryota; Incertae sedis; Amastigomonas

Pteridomonas danica AKA Actiinomonas mirabilis

Eukaryota; Chromoalveolata; Stramenopiles; Dictyophyceae; Pedinllales

Paraphysomonas imperforata

Eukaryota; Chromoalveolata; Stramenopiles; Chrysophyceae; Chromolinales

*According to Adl. et al. J.Eukaryot. Microbiol. Vol. 52 2005

Page 4: Emerging Diversity Within Well-known Heterotrophic Flagellates Groups Revealed by Environmental Surveys

Ecology and Diversity of Aquatic Protists Advances and Methodologies

Page 5: Emerging Diversity Within Well-known Heterotrophic Flagellates Groups Revealed by Environmental Surveys

Ecology and Diversity of Aquatic Protists Advances and Methodologies

FOFO MOMOFAFA CoastalCoastal Open OceanOpen OceanMA

Page 6: Emerging Diversity Within Well-known Heterotrophic Flagellates Groups Revealed by Environmental Surveys

Ecology and Diversity of Aquatic Protists Advances and Methodologies

Clade I

Clade H

Clade D

Clade E

Clade B2

Clade G

Clade F1

Clade F2

Clade A

Clade B1

Clade C

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Ecology and Diversity of Aquatic Protists Advances and Methodologies

Clade 2

Clade 1

MACH 5

MACH 3

MACH 4

MACH 6

MACH 7

MACH 8

MACH 1

MACH 2

Clade 3

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Ecology and Diversity of Aquatic Protists Advances and Methodologies

Freshwater

Cafeteria

Bicosoeca

Caecitellus

BorokaHalocafeteria

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Ecology and Diversity of Aquatic Protists Advances and Methodologies

Well-known cultured HNF are not usually found in clone libraries. That indicates that novel taxa are dominant in the aquatic environments.

After combining sequences retrieved from different environmental studies new clusters are constituted. This is what we have defined as Emerging Diversity.

This and other related studies indicates that most of the HNF are not cultured. Is extremely important to increase the culturing effort and assume that an strong culturing bias is affecting the study of HNF.

Emerging diversity will probably appear in all protists lineages. A deep analysis of molecular surveys is needed to improve protists phylogeny.

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Resolving Species in Marine Protists4th to 5th of December 2008

Vanessa Balagué

Dr. FernandoUnrein

Dr. FabriceNot

IreneForn

RaquelRodríguez

Prof. Ramon Massana

thanks for

your attention