Electrophoresis brochure 2-2010 - In Vitro · ElectrophoresisSystems Injection Moulded...
Transcript of Electrophoresis brochure 2-2010 - In Vitro · ElectrophoresisSystems Injection Moulded...
Electrophoresis Systems
Electrophoresis Systems
� Horizontal Gel Systems� Vertical Slab Gel Systems� 2-D Electrophoresis Systems� Blotting Systems� Power Supplies� Gel Drying� Gel Documentation� Gel Cutting Tips� Transilluminators� Electrophoresis Reagents
www.alphalabs.co.uk
Electrophoresis Systems
� Injection Moulded Construction – durable, leak-proof environment for complete safety and long life
� Cassette Type Electrodes – inexpensive, easy to replace. Made of 99.99% corrosion resistant, pure platinum
� Electrical Safety – lid can be located in one way only. On removal, power is disconnected from the buffer chamber
� Multiple Gel Trays – eliminate the need for additional gel tanks
Horizontal Gel Systems …… clearly better resolution.
“Plug and go” gel casting
Casting dams allow gels to be rapidly cast externally while the Clarit-E™ unit is in use for gel running.
UV transparent Gel Scoops,available for each unit size,offer a safe and easy way totransport gels to a UVTransilluminator.
Flexicasters allow the castingof varying gel lengths simplyby locking the moveable dam.Adjustable feet and levelbubble allow gels of uniformthickness to be poured.
Trays are UV transparent.White PVC platform coverand red loading guides canbe optionally applied toenhance well visibility.Gel trays are free ofindentations or casting gatechannels, which can interferewith sample progression.
2
Casette Type Electrodes can be easily replaced by the userEasy Click Lid Removal
www.alphalabs.co.uk
The Clarit-E series of horizontal gel units offers the most
versatile solution for DNA and RNA agarose gel
electrophoresis currently available. All units offer
unsurpassed economy of gel and buffer volume, with
gel size and sample number versatility. This eliminates
the need for multiple gel tanks for changes in gel size
or application.
All units contain removable UV transparent trays.
Gel size and sample number requirements can be exactly
matched in each unit as systems are available with one,
two or three tray options (dependent on model). Easy to
use, leak proof ‘plug and go’ gel casting dams are
included as standard to allow gels to be rapidly cast whilst
the Clarit-E unit is in use for gel running. Traditional tape
casting can be used, should this be preferred.
Although lid connectors are compatible with most major
power supplies, adapters are available to provide
complete compatibility.
High efficiency horizontal gel cooling
Removable gel table allowsa Coolpack to sit above thegel in buffer offeringenhanced gel resolutionand faster run times.
All units are CE marked and are in conformity with the relevant CE SafetyDirectives: 73/23/EEC: LOW VOLTAGE DIRECTIVE: IEC 61010-1:2001. EN 61010-1:2001; 89/336 /EEC EMC DIRECTIVE: EN55011 Emissions, EN50082-1 Immunity.
Electrophoresis Systems
Extensive range of combsFour thicknesses, colour coded:Black: 0.75mm for ultra resolved bandsWhite: 1mm supplied as standardRed: 1.5mm for maximising sample volumeBlue: 2mm for maximising sample volume
Options for sample prep
Options for multichannel pipette loading
The number of samples can be maximised using combs withhigher numbers of teeth.
3
Retractable connectorscompatible with most majorpower supplies.
Corrosion resistant goldplated terminals
Mini Horizontal Gel Tank
Electrophoresis Systems
� Available with 7 x 7cm, 7 x 10cm or with both gel trays
� Economic low gel and buffer volumes
� Small lab bench footprint
Typical application: Designed for quick checks of low tomedium numbers of samples.
The Clarit-E Mini is the smallest unit in the range, designed tomaximise run economy without compromising versatility astwo tray options are available, and combs ranging frompreparative up to 16 samples. Maximum separation distanceis 9cm.
Gel dimensions (W x L) 7 x 7cm, 7 x 10cm
Unit dimensions (W x L x H) 21 x 9 x 9cm
Max. sample: 7 x 7cm tray 32 samples7 x 10cm tray 64 samples
Buffer volume 225ml
Combs available: No. of samples 1, 2, 4, 8MC, 8, 10, 12MC, 16
Thickness 0.75, 1, 1.5, 2mm
Technical Specifications:
Catalogue DescriptionNumber
EL1000 Clarit-E Mini, 7 x 7cm UV tray, 2 x 8 sample combs, loading guides & dams
EL1100 Clarit-E Mini, 7 x 10cm UV tray, 2 x 8 sample combs, loading guides & dams
EL1050 Clarit-E Mini, 7 x 7cm & 7 x 10cm UV tray, 2 x 8 sample combs, loadingguides & dams
EL1002 7 x 7cm UV tray
EL1102 7 x 10cm UV tray
Mini Horizontal Gel Tank
Ordering Information:
Components of Clarit-E EL1050 Buffer saver blocks are availablefor all units, which physicallyreduce the volume of a gelchamber and so reduce bufferrequirements, saving cost.
Midi Horizontal Gel Tank
� Available with 10 x 7cm, 10 x 10cm or with both gel trays
� Run up to 100 samples
� Low buffer volumes
� Ideal for rapid electrophoresis
Typical application: Ideal for quick checks of samples fromPCR and cloning.
Designed for routine checks of a medium number ofsamples over short to medium gel lengths. Wider than theClarit-E Mini it allows more samples to be resolved per gelwithout a significant increase in buffer or gel volumes.
Gel dimensions (W x L) 10 x 7cm, 10 x 10cm
Unit dimensions (W x L x H) 22 x 12.5 x 9cm
Max. sample: 10 x 7cm tray 50 samples10 x 10cm tray 100 samples
Buffer volume 300ml
Combs available: No. of samples 1, 2, 4, 8, 10MC, 12, 16, 20MC, 25
Thickness 0.75, 1, 1.5, 2mm
Technical Specifications:
Catalogue DescriptionNumber
EL1200 Clarit-E Midi, 10 x 7cm UV tray, 2 x 16 sample combs, loading guides & dams
EL1300 Clarit-E Midi, 10 x 10cm UV tray, 2 x 16 sample combs, loading guides & dams
EL1250 Clarit-E Midi, 10 x 7cm & 10 x 10cm UV tray, 2 x 16 sample combs, loadingguides & dams
EL1202 10 x 7cm UV tray
EL1302 10 x 10cm UV tray
Midi Horizontal Gel Tank
Ordering Information:
Loading Guides allow easy wellidentification and sampleloading.
Scoops for safe transfer of gels.
4 www.alphalabs.co.uk
Midi 96 Horizontal Gel TankThe Clarit-E Midi 96 isideal for separation ofPCR products, or DNAsamples containing onlya few bands, from 96-well microtitre plates andthermal cycler blocks.
Rapid Testing –96 wells can be castsimultaneously within asingle gel in a precise 96-well microplate configuration.
Rapid Loading – gels can be loaded quickly and easily by8-channel pipette.
Extended Separation – offset layout of the wells ensures alonger run length per well.
High Speed & High Throughput – 96 samples loaded andseparated in 15 to 30 minutes without loss of resolution.
Easy Sample Identification – exact configuration of 96-well microplates allows samples to be quickly and easilyidentified from original microplates.
Gel dimensions (W x L) 10 x 12cm
Unit dimensions (W x L x H) 22 x 12.5 x 9cm
Max. sample capacity: 96 samples plus 1 or 2 marker lanes
Buffer volume 300ml
Combs available: No. of samples 8MC + 1 marker lane8MC + 2 marker lanes
Thickness: 1.5, 2mm
Recommended power supply EL2720
Technical Specifications:
Catalogue DescriptionNumber
EL1350 Clarit-E Midi 96, UV tray, Comb block with 12 x 8 sample, 1mm thick combswith 1 marker lane, casting dams
EL1355 Clarit-E Midi 96, UV tray, Comb block with 12 x 8 sample, 1.5mm thick combswith 1 marker lane, casting dams
EL1360 Clarit-E Midi 96, UV tray, Comb block with 12 x 8 sample, 1mm thick combswith 2 marker lanes, casting dams
EL1365 Clarit-E Midi 96, UV tray, Comb block with 12 x 8 sample, 1.5mm thick combswith 2 marker lanes, casting dams
Midi 96 Horizontal Gel Tank
Ordering Information:
Catalogue DescriptionNumber
EL1370 Comb block for EL1350
EL1375 Comb block for EL1355
EL1380 Comb block for EL1360
EL1385 Comb block for EL1365
EL1386 8-sample, 1mm thick comb, 1 marker lane
EL1387 8-sample, 1.5mm thick comb, 1 marker lane
EL1388 8-sample, 1.5mm thick comb, 2 marker lanes
EL1389 8-sample, 1mm thick comb, 2 marker lanes
EL1390 Clarit-E Midi, 96 well tray
Accessories and Combs
MultiSUB4
The Clarit-E MultiSUB 4 combines high throughputelectrophoresis with the space and time-saving advantagesof a small, compact unit.
High Throughput – a maximum 1200 samples may beseparated in as little as 15 minutes in 4 vertically stacked geltrays.
Rapid Loading – multichannel-compatible combs and gelplate configurations compatible with microplates andthermal cycler blocks ensure rapid loading of DNA miniprepsand PCR products by 8-channel pipette.
Thicker Combs – double-sided 1.5mm thick combs allowmore sample volume to be loaded into each well.
3 Gel tray Options – available in 8 x 6, 8 x 12 and8 x 18cm (W x L) sizes for maximum flexibility.
High Speed – a compact gel tank maximises the intensity ofthe electric field for rapid separations.
Small Footprint – helps maximise bench space.
Gel dimensions, (W x L) 8 x 6cm, 8 x 12cm, 8 x 18cm
Unit dimensions (W x L x H) 28 x 9 x 9cm
Max. sample capacity 1200
Buffer volume 1800ml
Combs available : No. of samples 12/1 sample and 18/8 sample
Thicknesses 1.5mm
Recommended power supply EL2720
Technical Specifications:
Catalogue DescriptionNumber
EL1980 Clarit-E multiSUB4, multi-level gel chamber, includes 4 x 8 x 12cm UV trays,4 x 18/8-sample, 1.5mm thick combs
MultiSUB4
Ordering Information:
Catalogue DescriptionNumber
EL1982 8 x 6cm UV tray
EL1984 8 x 12cm UV tray
EL1986 8 x 18cm UV tray
EL1980-12/1-1.5 multiSUB4 12/1-sample, 1.5mm thick comb
EL1980-18/8-1.5 multiSUB4 18/8-sample, 1.5mm thick comb
EL1988 External caster for multiSUB4
Accessories and Combs
Electrophoresis Systems
5www.alphalabs.co.uk
Choice
Electrophoresis Systems
� Three tray options
� Run up to 210 samples
� Low buffer volumes
� Multichannel pipette compatible combs for speed loading
Typical application: Ideal for restriction fragment analysis,sample prep or checking of high numbers of samples.
The Clarit-E Choice offers a wide degree of versatility. Threetray options are available allowing the choice of one, two orall three gel length options at the time of purchase. The15cm total run length allows restriction fragment or otherclose MW sample bands to be easily separated and identified.
Gel dimensions, (W x L) 15 x 7cm, 15 x 10cm, 15 x 15cm
Unit dimensions (W x L x H) 26.5 x 17.5 x 9cm
Max. sample: 15 x 7cm tray 70 samples15 x 10cm tray 140 samples15 x 15cm tray 210 samples
Buffer volume 500ml
Combs available : No. of samples 1, 2, 4, 10, 10MC, 12, 14MC, 16MC,18MC, 20, 28MC, 30MC, 35
Thicknesses 0.75, 1, 1.5, 2mm
Technical Specifications:
Catalogue DescriptionNumber
EL1450 Clarit-E Choice, 15 x 7cm UV tray, 2 x 20 sample combs, loading guides & dams
EL1400 Clarit-E Choice, 15 x 10cm UV tray, 2 x 20 sample combs, loading guides & dams
EL1500 Clarit-E Choice, 15 x 15cm UV tray, 2 x 20 sample combs, loading guides & dams
EL1550 Clarit-E Choice Trio, 15 x 7, 10 & 15cm UV tray, 2 x 20 sample combs, loadingguides and dams
Choice
Ordering Information:
Maxi
� Available with 20 x 25cm, 20 x 20cm or 20 x 10cm gel trays
� Run up to 550 samples
� Low buffer volumes
� Ideal for extended separations
Typical application: Primarily designed for separating highnumbers of samples from PCR or cloning.
The Clarit-E Maxi allows ultra high-resolution separations overextended runs. Tray sizes correspond to standard blotter sizes.It also allows easy sample transfer onto a membrane forfurther analysis. Multichannel pipette compatible combsfacilitate speed loading.
Gel dimensions, (W x L) 20 x 10cm, 20 x 20cm, 20 x 25cm
Unit dimensions (W x L x H) 39.5 x 23 x 9cm
Max. sample: 20 x 10cm tray 200 samples20 x 20cm tray 450 samples20 x 25cm tray 550 samples
Buffer volume 1200ml
Combs available: No. of samples 1, 2, 4, 10, 16, 20MC, 25, 30, 36,40MC, 50
Thicknesses 0.75, 1, 1.5, 2mm
Technical Specifications:
Catalogue DescriptionNumber
EL1600 Clarit-E Maxi, 20 x 25cm UV tray, 2 x 20 sample combs, loading guides & dams
EL1620 Clarit-E Maxi, 20 x 20cm UV tray, 2 x 20 sample combs, loading guides & dams
EL1640 Clarit-E Maxi, 20 x 10cm UV tray, 2 x 20 sample combs, loading guides & dams
EL1660 Clarit-E Maxi, 20 x 20cm & 20 x 10cm UV tray, 2 x 20 sample combs, loadingguides and dams
Maxi
Ordering Information:
Catalogue DescriptionNumber
EL1452 15 x 7cm UV tray
EL1402 15 x 10cm UV tray
EL1502 15 x 15cm UV tray
Accessories
Catalogue DescriptionNumber
EL1642 20 x 10cm UV tray
EL1622 20 x 20cm UV tray
EL1602 20 x 25cm UV tray
Accessories
6
Clarit-E Choice Trio includes all 3tray sizes for optimum versatilityand value.
Casting dams allow gels to berapidly cast externally while theClarit-E unit is in use for gelrunning.
www.alphalabs.co.uk
Electrophoresis Systems
Screen� Available
with 26 x 16,26 x 24 and26 x 32cm orall three geltrays
� Run up to672 samples
� Low buffervolumes
� Multichannel pipette compatible combs for speed loading
Typical application: Ideal for rapid screening of very largenumbers of clonal or PCR samples or extended highresolution separations.
The Clarit-E Screen allows loading and analysis of exactlyseven 96 well format micro titre plates.
The large gel run length of up to 32cm also allowsresolution of samples over a long distance for separation ofcomplex sample bands such as in restriction fragmentanalysis. Buffer recirculation ports are included to allowenhanced resolution over extended runs.
Ordering Information:
Catalogue DescriptionNumber
EL1800 Clarit-E Screen, 26 x 32cm UV tray, 6 x 28 sample combs, loading guides & dams
EL1820 Clarit-E Screen, 26 x 24cm UV tray, 6 x 28 sample combs, loading guides & dams
EL1840 Clarit-E Screen, 26 x 16cm UV tray, 6 x 28 sample combs, loading guides & dams
EL1860 Clarit-E Screen, 26 x 16, 24, & 32cm UV tray, 6 x 28 sample combs, loadingguides and dams
Screen
Fast Mini
� A convenient self-contained solution for routine agarosegel electrophoresis
� Ultra compact
� Low buffer and gel volumes
� Multichannel pipette compatible combs for speed loading
Typical application: For routine, rapid checking of samples.
This completely self contained system allows gel casting,running and analysis to be performed in the same unit. Theparallel electrode arrangement allows ultra efficient currenttransfer, enabling resolution to be completed within 30minutes. The UV transparent base allows direct viewing on aUV Transilluminator with no need for time-consumingtransfer and potential gel damage. Dual comb slots allowthe loading of up to 40 samples per gel.
Gel dimensions, (W x L) 10 x 8cm
Unit dimensions (W x L x H) 15 x 15 x 4cm
Max. sample capacity 40 samples
Buffer volume 50ml
Combs available: No. of samples 1, 4, 8, 12, 16, 20MC
Thicknesses 1, 1.5mm
Technical Specifications:
Catalogue DescriptionNumber
EL1700 Clarit-E Fast Mini, 10 x 8cm, 2 x 8 sample combs 1.5mm, and casting dams
EL1702 Fast Mini casting dams, pk/2
Fast Mini
Ordering Information:
Gel dimensions (W x L) 26 x 32cm, 26 x 24cm, 26 x 16cm
Unit dimensions (L x W x H) 50 x 28 x 9cm
Max sample: 26 x 32cm tray 672 samples26 x 24cm tray 504 samples26 x 16cm tray 336 samples
Buffer volume 1400ml
Combs available No. of samples 28MC, 56MC
Thicknesses 0.75, 1, 1.5, 2mm
Technical Specifications:
All Clarit-E Screen combs aremultichannel pipette compatible.
Catalogue DescriptionNumber
EL1802 26 x 32cm UV tray
EL1822 26 x 24cm UV tray
EL1842 26 x 16cm UV tray
Accessories
7www.alphalabs.co.uk
Accessories forHorizontal Units
Electrophoresis Systems
Presented here in an easy to read table, simply choose the geltank of interest across the top, and the accessory requiredfrom the left hand side, then look across for the appropriatecatalogue number.
Gel Levelling TableThe gel levelling table provides auseful way of pouring gels ofuniform thickness. The tablefeatures a 32 x 26cm platformwith large thumbwheel levellingfeet at each corner, as well as a levelling bubble.High Efficiency Horizontal Gel Cooling
� Effective cooling
� Cools without external chilling systems
� Improves gel resolution and reduces run times
Comprising a removable GelPlatform and CoolPack thisunique system will cool thebuffer chamber of Clarit-Ehorizontal gel units without anyexternal chilling. Simply placethe platform over the running tray and place a pre-chilledCoolPack on top. This allows the CoolPack to sit above thegel in buffer directly removing any heat generated.
Buffer Saver BlocksBuffer saver blocks are placedinto the positive and negativecompartments of a gel chamberand physically reduce the volumeof buffer required, saving cost.Blocks are available for all Clarit-E Horizontal Gel Units.
Accessories Mini Midi Choice Maxi Screen
Positive electrode EL1006 EL1206 EL1406 EL1606 EL1808
Negative electrode EL1008 EL1208 EL1408 EL1608 EL1810
Casting Dams EL1004 EL1204 EL1404 EL1604 N/A
Adhesive loading guides EL1010 EL1210 EL1410 EL1610 EL1804
Viewing platform EL1012 EL1212 EL1412 EL1612 EL1806
Cool pack and platform EL1014 EL1214 EL1414 EL1614 EL1812
Buffer saver blocks EL1016 EL1216 EL1416 EL1616 EL1814
Flexi Caster EL1018 EL1018 EL1418 EL1418 EL1816
Gel scoop EL1020 EL1220 EL1420 EL1624 EL1818
Gel Levelling Table EL1026 EL1026 EL1026 EL1026 EL1026
8
Electrode CasettesCasette type electrodes areinexpensive and easy to replace.Made of 99.99% pure corrosionresistant platinum.
Casting DamsCasting dams allow gels to berapidly cast externally whilstthe gel tank is in use.
Adhesive LoadingGuidesAllow easy well identificationand sample loading.
Viewing PlatformThe white PVC platform cover can be optionally applied toenhance well visibility.
Flexi CastersAllows gels to be cast of variouslengths simply by locking themoveable dam. Adjustable feetand bubble level ensure gels areof uniform thickness.
Gel ScoopsUV transparent gel scoops,available for each unit size,offer a safe and easy way totransport gels.
Ordering Information:
www.alphalabs.co.uk
Electrophoresis Systems
Combs for HorizontalGel Systems
Combi-Comb/Loading Guides
� Adjustable Height
� Options for sample preparation – described as “Prep”
� Options for multi-channel pipette compatibility – Lookfor “MC” in the description and catalogue number
0.75mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness
No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. SampleVolume Volume Volume Volume5mm 5mm 5mm 5mm
thick gel thick gel thick gel thick gel
Prep 1, Marker 1 EL1000-1-0.75 152µl EL1000-1-1 203µl EL1000-1-1.5 304µl EL1000-1-2 405µl
Prep 2, Marker 2 EL1000-2-0.75 68µl EL1000-2-1 90µl EL1000-2-1.5 135µl EL1000-2-2 180µl
Prep 4, Marker 2 EL1000-4-0.75 36µl EL1000-4-1 48µl EL1000-4-1.5 72µl EL1000-4-2 96µl
8 MC EL1000-8MC-0.75 8µl EL1000-8MC-1 11µl EL1000-8MC-1.5 17µl EL1000-8MC-2 23µl
8 EL1000-8-0.75 19µl EL1000-8-1 25µl EL1000-8-1.5 37µl EL1000-8-2 50µl
10 EL1000-10-0.75 14µl EL1000-10-1 18µl EL1000-10-1.5 27µl EL1000-10-2 36µl
12 MC EL1000-12MC-0.75 10µl EL1000-12MC-1 14µl EL1000-12MC-1.5 20µl EL1000-12MC-2 27µl
16 EL1000-16-0.75 7µl EL1000-16-1 10µl EL1000-16-1.5 15µl EL1000-16-2 20µl
Clarit-E Mini Horizontal Gel Tanks – EL1000, EL1050, EL1100 from page 4
� Simply add “LG” to the end of the product code for thecomb you want.
� Colour coded for thickness
� Comb and guide in one piece for convenience
� Adjustable height
These guides combine the convenience of a regular combwith the appropriate guide (same teeth number and width).For loading samples, simply flip over.
The number of samples can be maximised using high toothnumber combs.
0.75mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness
No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. SampleVolume Volume Volume Volume5mm 5mm 5mm 5mm
thick gel thick gel thick gel thick gel
Prep 1, Marker 1 EL1200-1-0.75 270µl EL1200-1-1 360µl EL1200-1-1.5 540µl EL1200-1-2 720µl
Prep 2, Marker 2 EL1200-2-0.75 118µl EL1200-2-1 158µl EL1200-2-1.5 236µl EL1200-2-2 315µl
Prep 4, Marker 2 EL1200-4-0.75 57µl EL1200-4-1 77µl EL1200-4-1.5 115µl EL1200-4-2 153µl
8 EL1200-8-0.75 30µl EL1200-8-1 41µl EL1200-8-1.5 61µl EL1200-8-2 81µl
10 MC EL1200-10MC-0.75 20µll EL1200-10MC-1 27µl EL1200-10MC-1.5 41µl EL1200-10MC-2 54µl
12 EL1200-12-0.75 17µl EL1200-12-1 23µl EL1200-12-1.5 34µl EL1200-12-2 45µl
16 EL1200-16-0.75 12µl EL1200-16-1 16µl EL1200-16-1.5 24µl EL1200-16-2 32µl
20 MC EL1200-20MC-0.75 10µl EL1200-20MC-1 14µl EL1200-20MC-1.5 20µl EL1200-20MC-2 27µl
25 EL1200-25-0.75 7µl EL1200-25-1 10µl EL1200-25-1.5 15µl EL1200-25-2 20µl
Clarit-E Midi Horizontal Gel Tanks – EL1200, EL1250, EL1300, EL1350, EL1355, EL1360, EL1365 from pages 4 and 5
Extensive range of combsFour thicknesses, colour coded:Black: 0.75mm for ultra resolved bandsWhite: 1mm supplied as standardRed: 1.5mm for maximising sample volumeBlue: 2mm for maximising sample volume
9www.alphalabs.co.uk
Electrophoresis Systems
0.75mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness
No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. SampleVolume Volume Volume Volume5mm 5mm 5mm 5mm
thick gel thick gel thick gel thick gel
Prep 1, Marker 1 EL1400-1-0.75 371µl EL1400-1-1 495µl EL1400-1-1.5 743µl EL1400-1-2 990µl
Prep 2, Marker 2 EL1400-2-0.75 169µl EL1400-2-1 225µl EL1400-2-1.5 338µl EL1400-2-2 450µl
Prep 4, Marker 2 EL1400-4-0.75 91µl EL1400-4-1 122µl EL1400-4-1.5 182µl EL1400-4-2 243µl
10 EL1400-10-0.75 34µl EL1400-10-1 45µl EL1400-10-1.5 68µl EL1400-10-2 90µl
12 EL1400-12-0.75 30µl EL1400-12-1 41µl EL1400-12-1.5 61µl EL1400-12-2 81µl
20 EL1400-20-0.75 16µl EL1400-20-1 21µl EL1400-20-1.5 32µl EL1400-20-2 43µl
35 EL1400-35-0.75 7µl EL1400-35-1 10µl EL1400-35-1.5 15µl EL1400-35-2 20µl
10 MC EL1400-10MC-0.75 22µl EL1400-10MC-1 29µl EL1400-10MC-1.5 44µl EL1400-10MC-2 59µl
14 MC EL1400-14MC-0.75 22µl EL1400-14MC-1 29µl EL1400-14MC-1.5 44µl EL1400-14MC-2 59µl
16 MC EL1400-16MC-0.75 20µl EL1400-16MC-1 27µl EL1400-16MC-1.5 41µl EL1400-16MC-2 54µl
18 MC EL1400-18MC-0.75 8µl EL1400-18MC-1 11µl EL1400-18MC-1.5 17µl EL1400-18MC-2 23µl
28 MC EL1400-28MC-0.75 8µl EL1400-28MC-1 11µl EL1400-28MC-1.5 17µl EL1400-28MC-2 23µl
30 MC EL1400-30MC-0.75 9µl EL1400-30MC-1 13µl EL1400-30MC-1.5 19µl EL1400-30MC-2 25µl
Clarit-E Choice Horizontal Gel Tanks – EL1400, EL1450, EL1500, EL1550 from page 6
0.75mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness
No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. SampleVolume Volume Volume Volume5mm 5mm 5mm 5mm
thick gel thick gel thick gel thick gel
Prep 1, Marker 1 EL1600-1-0.75 506µl EL1600-1-1 675µl EL1600-1-1.5 1013µl EL1600-1-2 1350µl
Prep 2, Marker 2 EL1600-2-0.75 236µl EL1600-2-1 315µl EL1600-2-1.5 473µl EL1600-2-2 630µl
Prep 4, Marker 2 EL1600-4-0.75 115µl EL1600-4-1 153µl EL1600-4-1.5 230µl EL1600-4-2 306µl
10 EL1600-10-0.75 54µl EL1600-10-1 72µl EL1600-10-1.5 108µl EL1600-10-2 144µl
16 EL1600-16-0.75 30µl EL1600-16-1 41µl EL1600-16-1.5 61µl EL1600-16-2 81µl
20 MC EL1600-20MC-0.75 20µl EL1600-20MC-1 27µl EL1600-20MC-1.5 41µl EL1600-20MC-2 54µl
25 EL1600-25-0.75 16µl EL1600-25-1 21µl EL1600-25-1.5 32µl EL1600-25-2 42µl
30 EL1600-30-0.75 13µl EL1600-30-1 17µl EL1600-30-1.5 26µl EL1600-30-2 34µl
36 EL1600-36-0.75 11µl EL1600-36-1 14µl EL1600-36-1.5 22µl EL1600-36-2 29µl
40 MC EL1600-40MC-0.75 8µl EL1600-40MC-1 11µl EL1600-40MC-1.5 17µl EL1600-40MC-2 23µl
50 EL1600-50-0.75 8µl EL1600-50-1 10µl EL1600-50-1.5 16µl EL1600-50-2 21µl
Clarit-E Maxi Horizontal Gel tanks – EL1600, EL1620, EL1640, EL1660 from page 6
0.75mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness
No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. SampleVolume Volume Volume Volume5mm 5mm 5mm 5mm
thick gel thick gel thick gel thick gel
28 MC EL1800-28MC-0.75 25µl EL1800-28MC-1 34µl EL1800-28MC-1.5 51µl EL1800-28MC-2 68µl
56 MC EL1800-56MC-0.75 10µl EL1800-56MC-1 14µl EL1800-56MC-1.5 20µl EL1800-56MC-2 27µl
Clarit-E Screen Horizontal Gel Tank – EL1800, EL1820, EL1840, EL1860 from page 7
1.0mm thickness 1.5mm thickness
No. of Wells Catalogue No. Sample Catalogue No. SampleVolume Volume5mm 5mm
thick gel thick gel
1, 1 marker EL1700-1-1 330µl EL1700-1-1.5 495µl
4 EL1700-4-1 90µl EL1700-4-1.5 135µl
8 EL1700-8-1 40µl EL1700-8-1.5 60µl
12 EL1700-12-1 25µl EL1700-12-1.5 38µl
16 EL1700-16-1 15µl EL1700-16-1.5 23µl
20 MC EL1700-20MC-1 10µl EL1700-20MC-1.5 15µl
Clarit-E Fast Mini horizontal Gel tank – EL1700 from page 7
10 www.alphalabs.co.uk
Electrophoresis Systems
Comet Assay Tanks
Cellulose Acetate System
� For Single Cell GelElectrophoresis
� Minimise exposure to lightand reduce background DNAdamage
� High efficiency cooling forenhanced resolution
The Clarit-E COMET assay tanksare specialised electrophoresisunits for Single Cell GelElectrophoresis. The units arespecifically designed to minimise exposure to light and reducebackground DNA damage. Previously, units designed forstandard electrophoresis have been used which with theirclear construction have not offered an easy to use system.
Constructed from Ebony acrylic to prevent UV degradation,the slides sit on a cooling platform which can be connectedto a water supply or chiller for active temperature controland cooling.
Catalogue DescriptionNumber
EL1900 Clarit-E Comet assay tank for 10 slides
EL1920 Clarit-E Comet assay tank for 20 slides
EL1940 Clarit-E Comet assay tank for 40 slides
EL1960 Clarit-E Comet assay tank for 80 slides
Comet Assay Tanks
Ordering Information:
� Compact High ResolutionSystems for ClinicalElectrophoresis
� Can accommodate celluloseacetate strips up to 24 x 20cm
� Complete range of cellulose acetate gels and kits
� Range includes Densitometer, Scanner and Software
Typical application: Clinical Electrophoresis Tests: haemoglobin,serum proteins for monoclonal gammopathies, urine proteins,isoenzymes, lipo and glycoproteins and for immunofixation.
Cellulose acetate electrophoresis is an important technique inclinical diagnostics. This range offers a complete systemsolution for research and clinical procedures.
The Clarit-E Cellulose Acetate system is the ideal tank for bothstandard and wet cellulose acetate electrophoresis. The unitincludes an adjustable support which enables easy and fastadjustment for different lengths of cellulose acetate strip.
11
Cellulose Acetate Gels – a complete range of wet celluloseacetate gels, which can be used for a wide variety of clinicalelectrophoresis applications.
CellasGEL cellulose acetate gels are available in a variety of sizes andapplications:- 2.5 x 14cm, 2.5 x 17cm, 5.7 x 14cm, 5.7 x 17cm, 5 x24cm, 17 x 17cm, 6 x 7.6cm Helena, 5.7 x 10.2 arched.
Kits are available for multiple tests on serum proteins,haemoglobin, urine proteins and for immunofixation.
Applicators – a variety of rapid sample applicators for aselection of applications:
� Two specimen applicator for semi micro samples onstrips 2.5cm wide
� Four specimen applicator for semi micro samples onstrips 5.7cm wide
� Four specimen applicator for haemoglobin samples onstrips 5.7cm wide
� Six specimen applicator for samples on strips 5.7cm wide
Bridges are available in a variety of formats for cellulose acetatesheets and for arched and horizontal Helena types of gels.
Densitometer – a universal densitometer scanner andsoftware, able to read 144 tests per minute.
Strip dimensions 24 x 20cm (W x L)
Unit dimensions 30 x 30cm
Technical Specifications:
Catalogue DescriptionNumber
EL6000 Clarit-E cellulose acetate electrophoresis unit
EL6047 Glob Al Scan software densitometer (computer and scanner not included)
EL6048 Flatbed scanner for Glob Al Scan
Cellulose Acetate System
Ordering Information:
Catalogue DescriptionNumber
EL6002 2.5 x 14 CellasGEL 250 micron
EL6004 2.5 x 14 CellasGEL 200 micron
EL6006 2.5 x 17 CellasGEL 200 micron
EL6008 5.7 x 14 CellasGEL 250 micron
EL6010 5.7 x 14 CellasGEL 200 micron
EL6018 17 x 17 CellasGEL 200 micron
EL6034 Applicator Semimicro 2+2 for CellasGEL 2.5 x 14cm
EL6038 Applicator Micro 8 for CellasGEL
EL6051 Bridge 11cm for CellasGEL sheets 17 x 17cm
EL6054 Bridge 11cm for CellasGEL strips 5.7 x 17cm
Other products are available in this range, please enquire
Accessories
www.alphalabs.co.uk
Electrophoresis Systems
� Rapid Gel Casting and Loading – ensures trouble free gel set-up
� Versatile – modules for slab gel, 2-D electrophoresis & electroblotting using a single universal buffer tank
Vertical Electrophoresis Systems …… clearly better resolution.
Simple, rapid, leak proof gel casting (unique sliding gates gel clamping system is only applicable for Mini vertical tank)
Insert glass plates and slidegates to create efficient seal
Transfer to casting base andtighten cams
Insert gel solution and comband allow to polymerise
Transfer to tank, fill withbuffer and run
Pressure clamping frame
Extensive range of combsUp to five thicknesses, colour coded:Purple: 0.5mm for ultra resolved bandsBlack: 0.75mm for ultra resolved bandsWhite: 1mm supplied as standardRed: 1.5mm for maximising sample volumeBlue: 2mm for maximising sample volume
Options for sample prep
Options for multichannel pipette loading
The number of samples can be maximised using combs withhigher numbers of teeth.
On the Mini gel tank a unique singlepiece pressure frame offers the optionof rapid single screw per side or newscrew free set up. Even pressure andbuffer contact across the platesresults in even sample migration andefficient heat removal from the gel.Glass plates are 2mm thick for Miniand 4mm thick for Mini Wide andMaxi tanks to reduce breakage.
A simple to use cooling pack systemensures enhanced resolution withoutcostly and time-consuming additionalequipment. No chiller, tap orobstructing connecting leads arerequired. The cooling pack is simplypre-chilled in a freezer and placed inthe gel tank. Additionally, the use ofcooling packs reduces buffer volume.
Run up to four gels at a time
While most vertical gel units can run only one or two gels, theClarit-E Mini units can run one, two or up to four gels at any timeusing a triple glass plate sandwich.
Effective buffer cooling
12 www.alphalabs.co.uk
The Clarit-E range of Vertical Gel Units combines ease of use
with high resolution separations. Three sizes, Mini 10 x
10cm, Mini Wide 20 x 10cm and Maxi 20 x 20cm, share a
host of common features including a guaranteed leak-proof
seal required for trouble free, rapid and uncomplicated gel
casting. Utilising a built in gel running module eliminates
time-consuming transfer of glass plates following casting, a
process which can cause gel damage and misalignment.
Glass plates with permanently bonded spacers, guarantee
perfect spacer alignment. The glass plate sandwich is simply
inserted between pressure bars and a minimum of screws
tightened. For the Clarit-E Mini there are no screws to
tighten as the unique gel clamping system ensures fast set
up times and guaranteed leak-proof gel casting. Following
polymerisation, the gel running module is simply inserted
into the gel tank for electrophoresis.
Electrophoresis Systems
Interchangeable Modules
Running Module 2D Module
Blotting Module withdouble-hinged cassettes foradded convenience
Corrosion resistant, 99.99%pure platinum electrodesand gold plated terminals –easily replaced, ensuringminimum repair times.
Retractable connectorscompatible with most majorpower supplies.
13
All units are CE marked and are in conformity with the relevant CE SafetyDirectives: 73/23/EEC: LOW VOLTAGE DIRECTIVE: IEC 61010-1:2001. EN 61010-1:2001; 89/336 /EEC EMC DIRECTIVE: EN55011 Emissions, EN50082-1 Immunity.
www.alphalabs.co.uk
Typical application: Mini SDS PAGE,Native PAGE, Gradient, Seconddimension and Nucleic acid separations.
The preferred unit for routine miniprotein electrophoresis, the Clarit-EMini is constructed using the latestinjection moulding manufacturingtechniques. This gives a high quality,low cost product with unsurpassedfinish, durability and strength.
The running insert has the option of sliding gates or a singlescrew per side for clamping gels.
Accessory electroblotting and tube gel modules are availablewhich use the same outer tank and lid. See Modular systemsfor further information.
Mini
Electrophoresis Systems
� InjectionMouldedconstruction
� Compatible withall 8 x 10 and10 x 10cmprecast gels
� Low buffervolumes
� Run up to 4 gelsPlate dimensions, 10 x 10cm
Gel dimensions, (W x L) 7.5 x 8cm
Unit dimensions (W x D x H) 19 x 13 x 15cm
Max. sample capacity 80 samples, per unit20 samples per gel
Buffer volume Min 250ml; Max 1200ml
Combs available : No. of samples 1, 5, 8MC, 9, 10, 12, 16MC, 20
Thicknesses 0.75, 1, 1.5, 2mm
Technical Specifications:
Catalogue DescriptionNumber
EL2004 Clarit-E Mini, 10 x 10cm includes glass plates with bonded 1mm thick spacers,2 x 12 sample combs, cooling pack, dummy plate.
EL2000 Clarit-E Mini, 10 x 10cm includes glass plates with bonded 1mm thick spacers,2 x 12 sample combs, cooling pack, dummy plate and casting base.
EL2012 Clarit-E Mini, 10 x 10cm includes glass plates with bonded 1mm thick spacers,2 x 12 sample combs, cooling pack, dummy plate, casting base and externalcasting upstand.
Mini
Ordering Information:
Clarit-E Mini and optionalaccessories
Internal running module
Multiple Mini Gel Casting
� Integral levelling feet and level bubble
� Standard and gradient gels can be poured
� Choice of 2, 6, 12 or 24 gel casting models
Advance casting of multiple mini gels can help to achieveconsistent results between runs. These multiple gel castingsystems are tailored for use with Clarit-E Mini verticalelectrophoresis units; with three models of 2, 6, 12 and 24gel capacities. Fewer gels can be poured if required using theacrylic saver blocks supplied with each system.
The fixed hinged clamps allows the gel sandwich to beadjusted to the correct pressure irrespective of the number orthe thickness of gels being poured. Separation sheets allowthe easy separation of the glass plates after pouring.
Catalogue DescriptionNumber
EL2018 2 gel caster for 8 x 10cm or 10 x 10cm gels
EL2164 6 gel caster for 8 x 10cm or 10 x 10cm gels
EL2166 12 gel caster for 8 x 10cm or 10 x 10cm gels
EL2168 24 gel caster for 8 x 10cm or 10 x 10cm gels
Multiple Mini Gel Casting
Ordering Information:
14
Gel loading guides
www.alphalabs.co.uk
Mini Wide
� Offers the capacity of two mini gelson a single gel
� Optional low or high buffer volumes
Typical application: Mini SDS PAGE,Native PAGE, Gradient, Seconddimension and Nucleic acid separations.
The Clarit-E Mini Wide vertical gel unit with a gel width of20cm effectively allows double the number of samples to beresolved as the Clarit-E Mini unit. This allows consistency ofsample comparison on a single gel and is designed for thosewith greater than 20 samples to compare and resolve.
Both the Mini Wide and the Maxi system use 4mm thick glassplates which reduce breakage and have bonded spacers foradded convenience. A wide range of accessories is availableallowing many techniques to be performed using the sameunit. Prep combs can be used to maximise sample loadingand recovery.
Uses same combs as Maxi systems
Catalogue DescriptionNumber
EL2083 Clarit-E Mini Wide, 20 x 10cm 2 sets of glass plates with bonded 1mm thickspacers, 2 x 24 sample combs, cooling pack
EL2084 Clarit-E Mini Wide, 20 x 10cm 2 sets of glass plates with bonded 1mm thickspacers, 2 x 24 sample combs, cooling pack including casting base
EL2088 Clarit-E dual caster, 20 x 10cm
Mini Wide
Ordering Information:
Maxi
� Ideal for high resolution separations
� Optional low or high buffer volumes
Typical application: Maxi SDS PAGE, Native PAGE, Gradient,Second dimension and Nucleic acid separations.
The preferred unit for maxi protein electrophoresis, the Clarit-EMaxi unit combines convenient ease of use features with highresolution separations.
Electrophoresis Systems
Plate dimensions 20 x 10cm
Gel dimensions (W x L) 18 x 8cm
Unit dimensions (W x D x H) 26 x 16 x 16cm
Max. sample capacity 192 samples per unit48 samples per gel
Buffer volume Min 600ml; Max 2800ml
Combs available : No. of samples 1, 5, 10, 18MC, 24, 30, 36MC, 48
Thicknesses 0.75, 1, 1.5, 2mm
Technical Specifications:
Catalogue DescriptionNumber
EL2120 Clarit-E Maxi, 20 x 20cm dual with glass plates with bonded 1mm thickspacers, 2 x 24 sample combs, cooling pack, dummy plate
EL2100 Clarit-E Maxi, 20 x 20cm dual with glass plates with bonded 1mm thickspacers, 2 x 24 sample combs, cooling pack, dummy plate and casting base
EL2110 Clarit-E dual caster, 20 x 20cm
Maxi
Ordering Information:
Plate dimensions 20 x 20cm
Gel dimensions (W x L) 16 x 17.5cm
Unit dimensions (W x D x H) 26 x 16 x 28cm
Max. sample capacity 192 samples per unit48 samples per gel
Buffer volume Min 1200ml; Max 5600ml
Combs available : No. of samples 1, 5, 10, 18MC, 24, 30, 36MC, 48
Thicknesses 0.75, 1, 1.5, 2mm
Technical Specifications:
15www.alphalabs.co.uk
Maxi Plus for Second Stage 2-D
Electrophoresis Systems
The 26cm active gel widthprovides a large gel area toresolve large IEF strips. Incombination with the IEFsystem, this offers a completepackage for 2-Delectrophoresis. The unitutilises the Clarit-E advanceddesign features to provideconvenient ease of use withhigh resolution separations.
Rapid set up cooling retains resolution in extended separationsand also saves on buffer volume without affecting run quality.Four gels can be resolved per run. A wide range of accessoriesis available to allow easy transition between 2-D and standardvertical electrophoresis techniques. In particular different typesof 2-D comb allow a wide degree of versatility in sampleselection and gel set-up.
Unit dimensions (W x H x D) 36 x 33 x 18cm
Plate dimensions (W x L) 30 x 22cm
Gel dimensions (W x L) 28 x 20cm
Max sample 300 samples per run;75 samples per gel
Buffer volume Min 1800ml, Max 8400ml
Combs available: No. of teeth 1, 2, 4, 28MC, 56MC, 75
Thicknesses 0.25, 0.35, 0.5, 1, 1.5, 2.0mm
Technical Specifications:
Catalogue DescriptionNumber
EL2390 Clarit-E Maxi Plus, 30 x 22cm dual with glass plates with bonded 1.5mm spacers,2 x 28 sample combs, 2 x 2-D sample combs, cooling pack, dummy plate
EL2395 Clarit-E Maxi Plus, 30 x 22cm dual with glass plates with bonded 1.5mm spacers,2 x 28 sample combs, 2 x 2-D sample combs, cooling pack, dummy plate and casting base
EL2334 Clarit-E Maxi Plus blot insert - includes 3 cassettes and 6 fibre pads
EL2318 Clarit-E dual caster – Maxi Plus
Maxi Plus for second stage 2-D
Ordering Information:
DNA Sequencing Unit� Run up to 96 samples
� Enhanced gel heathomogenisation
� Variable low or highbuffer volumes
� 20 x 50cm or 33 x45cm formats
Typical application: DNASequencing, 2-D analysis,Micro-satellite analysis,DNA fingerprinting, Gelshift assays, Single- Strand Conformation Polymorphism(SSCP), Heteroduplex and Oligonucleotide analysis.
Ideal for a variety of large formatvertical gel applications, these unitsoffer advanced features forenhancing gel resolution and easeof use, essential when handlinggels of this size. Each unit containsultra soft silicone seals for easyplate sealing and trouble-free runs,even over extended run times.
Resolution is enhanced by using an aluminium heat sink plate,which is essential for even sample migration.
Added convenience is provided by a removable lower buffertank and upper buffer drainage tap.
Catalogue DescriptionNumber
EL2560 Clarit-E DNA sequencing unit, 20cm wide, glass plates, 0.35mm spacers,48 sample comb
EL2580 Clarit-E DNA sequencing unit, 33cm wide, glass plates, 0.35mm spacers,48 sample comb
EL2594 Fan heater sensor kit for DNA sequencing unit EL2560 and EL2580
DNA Sequencing Unit
Ordering Information:
Plate dimensions (W x L) EL2560 20 x 50cmEL2580 33 x 45cm
Max sample capacity EL2560 48 samplesEL2580 96 samples
Buffer volume EL2560 Min 500ml, Max 1000mlEL2580 Min 800ml, Max 2000ml
Combs available No. of teeth 24, 48, 80, 96
Thicknesses 0.25, 0.35, 1, 1.5mm
Technical Specifications:
16
Special buffer chambers allow either low buffer volumes to beused for economy or high buffer volumes to be used forextended runs.
A wide range of interchangeable comb and spacer optionsallows a large number of techniques to be easily accomplished.
www.alphalabs.co.uk
Electrophoresis Systems
Combs for Clarit-E VerticalGel Systems
� Options for Sample Prep
� Options for multi channel pipette compatibility
The number of samples can be maximised using high toothnumber combs.
0.5mm thickness 0.75mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness
No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. SampleVolume Volume Volume Volume Volume5mm 5mm 5mm 5mm 5mm
thick gel thick gel thick gel thick gel thick gel
1 Prep, 1 Marker EL2000-1-0.5 350µl EL2000-1-0.75 500µl EL2000-1-1 650µl EL2000-1-1.5 1000µl EL2000-1-2 1300µl
5 EL2000-5-0.5 40µl EL2000-5-0.75 70µl EL2000-5-1 100µl EL2000-5-1.5 140µl EL2000-5-2 200µl
8 MC EL2000-8MC-0.5 20µl EL2000-8MC-0.75 40µl EL2000-8MC-1 60µl EL2000-8MC-1.5 80µl EL2000-8MC-2 120µl
9 EL2000-9-0.5 20µl EL2000-9-0.75 35µl EL2000-9-1 50µl EL2000-9-1.5 70µl EL2000-9-2 100µl
10 EL2000-10-0.5 20µl EL2000-10-0.75 30µl EL2000-10-1 40µl EL2000-10-1.5 30µl EL2000-10-2 80µl
12 EL2000-12-0.5 15µl EL2000-12-0.75 25µl EL2000-12-1 35µl EL2000-12-1.5 50µl EL2000-12-2 70µl
16 MC EL2000-16MC-0.5 15µl EL2000-16MC-0.75 20µl EL2000-16MC-1 25µl EL2000-16MC-1.5 40µl EL2000-16MC-2 50µl
20 EL2000-20-0.5 10µl EL2000-20-0.75 15µl EL2000-20-1 20µl EL2000-20-1.5 30µl EL2000-20-2 40µl
Clarit-E Vertical Mini – EL2000, EL2002, EL2004, EL2006, EL2008, EL2010, EL2012 from page 14
Extensive range of combs and spacersUp to five thicknesses, colour coded:Purple: 0.5mm for ultra resolved bandsBlack: 0.75mm for ultra resolved bandsWhite: 1mm supplied as standardRed: 1.5mm for maximising sample volumeBlue: 2mm for maximising sample volume
0.75mm thickness 1.0mm thickness 1.5mm thickness 2.0mm thickness
No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. SampleVolume Volume Volume Volume5mm 5mm 5mm 5mm
thick gel thick gel thick gel thick gel
1 Prep, 1 Marker EL2100-1-0.75 1100µl EL2100-1-1 1500µl EL2100-1-1.5 2200µl EL2100-1-2 3000µl
5 EL2100-5-0.75 160µl EL2100-5-1 200µl EL2100-5-1.5 320µl EL2100-5-2 400µl
10 EL2100-10-0.75 80µl EL2100-10-1 100µl EL2100-10-1.5 160µl EL2100-10-2 200µl
18 MC EL2100-18MC-0.75 40µl EL2100-18MC-1 50µl EL2100-18MC-1.5 80µl EL2100-18MC-2 100µl
24 EL2100-24-0.75 30µl EL2100-24-1 40µl EL2100-24-1.5 60µl EL2100-24-2 80µl
30 EL2100-30-0.75 25µl EL2100-30-1 35µl EL2100-30-1.5 50µl EL2100-30-2 70µl
36 MC EL2100-36MC-0.75 20µl EL2100-36MC-1 25µl EL2100-36MC-1.5 40µl EL2100-36MC-2 50µl
48 EL2100-48-0.75 15µl EL2100-48-1 20µl EL2100-48-1.5 30µl EL2100-48-2 40µl
Clarit-E Vertical Maxi Plus – EL2390, EL2395 from page 16
Clarit-E Vertical Mini Wide/Maxi – EL2083, EL2084, EL2100, EL2120 from page 15
17
1.0mm thickness 1.5mm thickness
No. of Wells Catalogue No. Sample Catalogue No. SampleVolume Volume5mm 5mm
thick gel thick gel
1 Prep, 1 Marker EL2390-1-1 2250µl EL2390-1-1.5 3375µl
2 EL2390-2-1 1125µl EL2390-2-1.5 1680µl
4 EL2390-4-1 550µl EL2390-4-1.5 825µl
28 MC EL2390-28MC-1 80µl EL2390-28MC-1.5 120µl
56 MC EL2390-56MC-1 40µl EL2390-56MC-1.5 60µl
75 MC EL2390-75MC-1 25µl EL2390-75MC-1.5 37µl
Combs also available in other thicknesses and number, please enquire
www.alphalabs.co.uk
Loading guides are available forsome comb options, please enquire
Combs for Clarit-E 20cm wide large vertical format unit EL2560 from page 16
Electrophoresis Systems
Combs for Clarit-E 33cm wide large vertical format unit EL2580 from page 16
18
0.25mm thickness 0.35mm thickness 1mm thickness 1.5mm thickness
No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. SampleVolume Volume Volume Volume5mm 5mm 5mm 5mm
thick gel thick gel thick gel thick gel
24, Sharks tooth EL2560-24-0.25 7µl EL2560-24-0.35 9µl EL2560-24-1 40µl EL2560-24-1.5 60µl
48, Sharks tooth EL2560-48-0.25 3µl EL2560-48-0.35 5µl EL2560-48-1 20µl EL2560-48-1.5 30µl
0.25mm thickness 0.35mm thickness 1mm thickness 1.5mm thickness
No. of Wells Catalogue No. Sample Catalogue No. Sample Catalogue No. Sample Catalogue No. SampleVolume Volume Volume Volume5mm 5mm 5mm 5mm
thick gel thick gel thick gel thick gel
24, Sharks tooth EL2580-48-0.25 7µl EL2580-48-0.35 9µl EL2580-48-1 35µl EL2580-48-1.5 50µl
80, Sharks tooth EL2580-96-0.25 3µl EL2580-96-0.35 5µl
96, Sharks tooth EL2580-96-0.25 3µl EL2580-96-0.35 5µl
The list above includes the most popular combs for these units. Many more options are available, please enquire.
www.alphalabs.co.uk
Item Description Mini Mini Wide Maxi Maxi Plus 20cm seq. 33cm seq.
Cooling Pack EL2040 EL2140 EL2342
Notched Glass Plates EL2042 EL2112 EL2142 EL2364 EL2562 EL2582
Plain Glass Plates EL2044 EL2116 EL2144 EL2366 EL2564 EL2584
Plain Plates +0.5mm Spacers EL2046
Plain Plates +0.75mm Spacers EL2048 EL2118 EL2148 EL2374
Plain Plates +1mm Spacers EL2050 EL2121 EL2150 EL2378
Plain Plates +1.5mm Spacers EL2052 EL2122 EL2152 EL2382
Plain Plates +2mm Spacers EL2054 EL2125 EL2154 EL2384
Notched Plates +0.5mm Spacers EL2056
Notched Plates +0.75mm Spacers EL2058 EL2127 EL2158 EL2368
Notched Plates +1mm Spacers EL2060 EL2129 EL2159 EL2376
Notched Plates +1.5mm Spacers EL2062
Notched Plates +2mm Spacers EL2064
Dummy Plate EL2066 EL2156 EL2157 EL2386
Spacers -0.25mm EL2566 EL2586
Spacers -0.35mm EL2568 EL2588
Spacers -0.5mm EL2068 EL2068
Spacers -0.75mm EL2070 EL2070 EL2170 EL2388
Spacers -1mm EL2072 EL2072 EL2172 EL2392 EL2570 EL2590
Spacers -1.5mm EL2074 EL2074 EL2174 EL2394 EL2572 EL2592
Spacers -2mm EL2076 EL2076 EL2176 EL2396
A range of Glass Plates and Spacers at a glance
Accessories for Vertical Systems
Electrophoresis Systems
19www.alphalabs.co.uk
Isoelectric Focusing
� For IEF strip and gel applications
� Large cooling platform area
� Pick and place easy to position electrodes
� Grid for accurate electrode alignment
The IEF system has been designed to allow the full range ofIEF flat bed electrophoresis techniques. These include wickbased electrophoresis, precast horizontal IEF gelelectrophoresis, hand cast horizontal IEF Gel electrophoresisand Iso Electric focusing using IEF strips. The unit includesenhanced cooling over a large platform which offersincreased strip capacity and gel area.
Uniform cooling ofthe platform isachieved using a largeceramic plate whichcan be connected toany standard chillervia quick fitconnectors forenhanced gel or stripcooling.
Modular Systems
� Economy of bench space and cost
� Enhanced reproducibility
� Simple to use casting
� Rapid set up cooling
The Clarit-E range of Modular Vertical Gel Systems allowmultiple electrophoresis techniques to be performed in thesame unit. Using the same main tank and lid, three differentinserts are interchangeable for slab gel, tube gel andelectroblotting techniques. These systems are available inthree size options, Mini 10 x 10cm, Mini Wide 20 x 10cmand Maxi 20 x 20cm and are available as individual,combination or complete modular systems. Combinationand modular systems offer maximum value and space andhave the added benefit of increased consistency, whichresults from using the same universal base and lid.
Max strip length 26cm
Max gel dimensions 26 x 26cm
Unit dimensions (W x D x H) 55 x 35 x 10cm
Technical Specifications:
Catalogue DescriptionNumber
EL7000 Clarit-E Flatbed IsoElectric Focusing System 26 x 26cm
EL7010 Chiller
Isoelectric Focusing
Ordering Information:
Electrophoresis Systems
20 www.alphalabs.co.uk
Mini, Mini Wide and Maxicomplete Modular SystemsThese systems include all modules and accessories requiredfor Slab Gel Electrophoresis, 2-D Electrophoresis andElectroblotting. The central component is the Clarit-E Mini,Mini Wide or Maxi Vertical unit. These include a rapid andintuitive casting system, enhanced and easy to set up coolingsystem and have increased capacity – the Mini can run up tofour gels per run. In addition, the Clarit-E Tube gel module iscapable of resolving up to 10 first dimension gels and theElectroblotting module has a 3 blot capacity.
Complete Modular Systems include: Vertical tank with gelrunning module, 2x notched glass plates, 2x plain glassplates with bonded spacers, dummy plate, 2x combs, castingbase, silicone mat, cooling pack, running module for tubegels, capillary tubes, blanking plugs, 2-D combs, spacers,electroblotting module, 3x compression cassettes and 6xfibre pads.
2-D Complete Systems� Rapid set-up electrofocusing
� 10 tube capacity
� Extended accessory range
� Enhanced cooling features
The Clarit-E 2-D Systems includeboth modules required for SlabGel and First DimensionElectrophoresis and accessories, to provide a complete Mini,Mini Wide or Maxi 2-D system. The Tube Gel Module includes arapid release gasket for easy tube extraction. Focusing can beaccomplished in as little as three hours in the Mini Unit.Disposable capillary tubes are included for added convenience,plus 2-D combs and spacers, which are colour coded accordingto thickness for easy identification.
Complete 2-D System comprising: Vertical tank with gelrunning module, 2x notched glass plates, 2x plain glass plateswith bonded spacers, dummy plate, 2x combs, casting base,silicone mat, cooling pack, running module for tube gels,capillary tubes, blanking plugs, 2-D combs and spacers.
Tube Gel Units, Inserts andAccessoriesThese stand-alone, complete units for first dimensioncapillary/tube gel electrophoresis are available in 3 sizes, Mini10 x 10cm, Mini Wide 20 x 10cm and Maxi 20 x 20cmformats. Units are interchangeable with Clarit-E slab gel andelectroblotting inserts.
Modular Electroblotting� Hinged cassettes
� 3 blot capacity
� High intensity electrodes
� Rapid set-up cooling
The Clarit-E ElectroblottingSystems include both modulesrequired for Slab Gel Electrophoresis and Electroblotting andaccessories, to provide a complete Mini, Mini Wide or Maxi gelcasting, running and electroblotting system.
A high intensity current is generated by coiled platinumelectrodes. These in conjunction with the advanced coolingfeatures allow rapid electroblotting in as little as one hour.
Clarit-E Modular electroblotting system comprises: Vertical tankwith gel running module, 2x notched glass plates, 2x plainglass plates with bonded spacers, dummy plate, 2x combs,casting base, silicone mat, cooling pack, electroblottingmodule, 3x compression cassettes and 6x fibre pads.
Modules included in the Clarit-EMaxi 2-D system
Modules included in the Clarit-EMini Wide 2-D system
Clarit-E Tube Gel Units comprise tank and lid, glass capillarytubes, blanking ports and cooling pack.
Electroblotting Units� Ideal for Western
blotting ofproteins
� Open design, rigidcassettes formaximum currenttransfer
These stand-alone,complete units forelectroblotting, Mini,Mini Wide and Maxiformats, includeClarit-E tank and lid, electroblotting insert, cassettes and fibrepads. Units are interchangeable with Clarit-E slab and tube gelinserts. Hinged rigid cassettes allow rapid set up andconvenience and ensure even contact between the gel andmembrane is maintained.
Unit dimensions: Mini 19 x 13 x 15cm(W x D x H) Mini Wide 26 x 16 x 16cm
Maxi 26 x 16 x 28cm
Max. sample capacity: Mini Slab 80 samples, 20 samples /gel, Tube - 10 tubes; Blot - 3 blots
Mini Wide Slab 192 samples, 48 samples /gel,Tube - 10 tubes; Blot - 3 blots
Maxi Slab 192 samples, 48 samples /gel,Tube - 10 tubes; Blot - 3 blots
Buffer volume: Mini Min 250ml; Max 1200mlMini Wide Min 600ml; Max 2800mlMaxi Min 1200ml; Max 5600ml
Technical Specifications:
Catalogue Number Description
Mini Mini Wide Maxi Complete Electrophoresis System Includes tube gel,EL2200 EL2250 EL2300 blotting and vertical system
EL2320 EL2330 EL2340 Complete 2-D Electrophoresis System Includes tube and vertical system
EL2026 EL2096 EL2126 Tube Gel Unit, with capillary tubes, blanking portsand cooling pack
EL2028 EL2098 EL2128 Tube Gel Insert – includes glass tubesand blanking ports
EL2030 EL2030 EL2130 Capillary Tubes
EL2356 EL2356 Capillary Tubes 1.5mm
EL2032 EL2032 EL2032 Capillary Blanking Ports
EL2400 EL2450 EL2500 Complete Blotting System includes vertical system and electroblotting module
EL2402 EL2452 EL2502 Blotting System includes tank, 3 cassettes, 6 fibre pads and cooling pack
EL2034 EL2114 EL2134 Blotting insert includes 3 cassettes and 6 fibre pads
EL2036 EL2106 EL2136 Blotting cassette
EL2038 EL2458 EL2138 Fibre pads
Electroblotting Units
Ordering Information:
Accessories for Clarit-EVertical SystemsGradient MixersIdeal for Caesium, Sucrose and Gel gradients these GradientMixers comprise two chambers – a reservoir and a mixingchamber with an interconnecting valve. A second valveregulates the output flow from the mixing chamber. Allmixers have a flat base which allows them to be placed on amagnetic stirrer. A magnetic stirring bar can be placeddirectly in the mixing chamber to ensure a constant gradient.The support rod allows the mixer to be fixed to a retort standfor extra stability.
Catalogue DescriptionNumber
EL2600 15ml Gradient Mixer
EL2610 25ml Gradient Mixer
EL2615 50ml Gradient Mixer
EL2620 100ml Gradient Mixer
EL2625 500ml Gradient Mixer
Gradient Mixers
Ordering Information:
Electrophoresis Systems
Plate RacksThese sturdy racks are designedfor safe drying and storage ofglass plates. The small rack canhold up to 20x 2mm thick plateswhile the larger rack canaccommodate up to 10x 5mmthick glass plates.
Catalogue DescriptionNumber
EL2630 Mini glass plate rack for 20 x 2mm plates
EL2640 Large glass plate rack for 10 x 5mm plates
Plate Racks
Ordering Information:
21www.alphalabs.co.uk
Electrophoresis Systems
22 www.alphalabs.co.uk
Tank Sub Electroblotters� Ideal for wet
electroblotting ofproteins –Western blotting
� Up to five gel blotcassettes utilisedat any one time
� Hinged cassettesfor addedconvenience
� Accommodatesgel thicknessesfrom 0.25 up to3mm
Tank Sub Electroblotters offer a combination of increasedcapacity with economy saving features. Both units, Mini 10 x10cm and Maxi 20 x 20cm, have increased capacity overstandard systems. This is especially useful in high throughputlaboratories.
A uniform electric field is provided by a high intensity coiledelectrode and ensures uniform transfer across the blotsurface. The cassette’s open architecture ensures themaximum blot area allows direct transfer of current. Its rigidconstruction ensures contact between the gel and membraneis retained throughout the blot and an even pressure ismaintained. These units are compatible with magnetic stirrersto aid heat dispersal and prevent pH drifts in the buffer dueto incomplete buffer mixing. Each system includes a coolingpack to further enhance transfer efficiency by removingexcess heat. This also saves on buffer for added economy.
Catalogue DescriptionNumber
EL2412 Electro Blot Mini, 10 x 10cm System for five cassettes, with tank and lid,5x cassettes, 12x fibre pads and cooling pack
EL2512 Electro Blot Maxi, 20 x 20cm System for five cassettes, with tank and lid,5x cassettes, 12x fibre pads and cooling pack
EL2036 Tank Blot Mini Cassette
EL2038 Fibre pads - pk/6
EL2136 Tank Blot Maxi Cassette
EL2138 Fibre pads - pk/6
Tank Sub Electroblotters
Ordering Information:
Semi Dry Electroblotters
Catalogue DescriptionNumber
EL2460 Semi Dry Mini Blotter 10 x 10cm system
EL2465 Semi Dry Maxi Blotter 20 x 20cm system
EL2470 Semi Dry Maxi Plus Blotter 20 x 50cm system
EL2475 Semi Dry Maxi Long Blotter 33 x 45cm system
Semi Dry Electroblotters
Ordering Information:
Unit dimensions: Mini 19 x 13 x 19cm(W x D x H) Maxi 24 x 16 x 26cm
Max. sample capacity: Mini 5 Blots, 10 x 10cmMaxi 5 Blots, 20 x 20cm
20 Blots, 10 x 10cm
Buffer volume: Mini Min 1000ml; Max 1500mlMaxi Min 4300ml; Max 6000ml
Technical Specifications:
Unit dimensions: Mini 16 x 16 x 7cm(W x L x H) Maxi 26 x 26 x 7cm
Maxi Plus 39 x 51 x 7cmMaxi Long 26 x 56 x 7cm
Max. sample capacity: Mini 1 Blot, 10 x 10cmMaxi 1 Blot, 20 x 20cm
4 Blots, 10 x 10cmMaxi Plus 1 Blot, 35 x 45cmMaxi Long 1 Blot, 20 x 50cm
Buffer volume: Mini 5mlMaxi 20mlMaxi Plus 75mlMaxi Long 50ml
Technical Specifications:
� Rapid transfer times
� Western, Southern and Northern Blots
� Economic Transfers due to very low buffer volumes
� Screw down lid – Gels from 0.25 up to 10mm thick canbe blotted
These Semi Dry Blotters offer rapid transfer times for DNA,RNA and protein blotting - typically 15 to 30 minutes. Allunits can be used for all types of blotting via uncomplicatedbuffer and set up procedures. Each unit is compatible withtheir respective Clarit-E vertical gel system. The electrodes,comprising platinum coated anode and stainless steelcathode, will exhibit practically no corrosion and so providemany years of trouble free use. Uniform heat dispersion acrossthe blot sandwich ensures stable transfer times and no heatinduced sample loss or transfer distortions. Being translucent,it allows viewing of the blot sandwich to ensure correctpositioning and transfer is occurring correctly. Electrode platesare fully separated to prevent arching or damage.
Capillary Blotting
� Efficient transfer of high and low molecular weightfragments
� Fast, easy set-up
� DNA transfer in 2 hours or less; RNA transfer in 3 hoursor less
� Floating lid ensures even pressure without gelcompression
These Capillary Blotting systems offer a contained unit forsouthern and northern blotting. These units incorporateoptimized permanent wicks, which eliminate the requirementfor overlapping wicks, offering greater consistency.
The porous polyethylene platform draws up transfer bufferfaster than traditional systems allowing for faster transfer times.
Side by side transfer of multiple gels can be performed in asingle set up.
Available in 15 x 26cm and 28 x 26cm formats.
Principle: Through capillary action, the buffer travels fromthe reservoir to the absorbent towelling via the poroussupports, permanent wick, gel and membrane. This actionoptimises the transfer of low and high Molecular WeightDNA/RNA from gel to membrane without mechanical damageto even the thinnest of gels.
Unit dimensions: Mini 30 x 30 x 10cm
(W x D x H) Maxi 42 x 30 x 10cm
Technical Specifications:
Dot and Slot Blotters
� Low cost
� Simple construction
� Easy assembly
� Alphanumeric sample identification
Four different sample number size and styles of HybridisationManifold are offered: 48 or 96 well dot blotter and 24 or 48well slot blotters.
Typical applications include clone screening with DNA/RNAprobes in Southern/Northern blots and immunologicalscreening with antibodies in western blots.
The units incorporate precision lapped surfaces to ensureuniform blotting membrane contact and a leak proof gasket.These prevent lateral transfer of samples – smudging – byensuring that a complete vacuum is formed. Six thumbscrewsensure even and tight sealing for fast sample suck down.
Electrophoresis Systems
Catalogue DescriptionNumber
EL2448 48 well Dot Blot Manifold, 3 x 16 array
EL2496 96 well Dot Blot Manifold, 8 x 12 array
EL2524 24 well Slot Blot Manifold, 2 x 12 array
EL2548 48 well Slot Blot Manifold, 3 x 16 array
Dot and Slot Plotters
Ordering Information:
Catalogue DescriptionNumber
EL2485 Capillary Blotting Unit (15cm x 26cm) (Mini)
EL2480 Capillary Blotting Unit (28cm x 26cm) (Maxi)
Capillary Blotting
Ordering Information:
Model EL2448 EL2496 EL2524 EL2548
Configuration 3 x 16 8 x 12 2 x 12 3 x 16
Size of well 6mm diam. 6mm diam. 6 x 0.5mm 6 x 0.5m12mm deep 12mm deep 12mm deep 12mm deep
Vacuum required 600mg Hg 0.8 BAR with cold trap
Unit dimensions 60 x 95 60 x 105 60 x 74 60 x 95x 100cm x 140cm x 83cm x 100cm
Membranes 12.1 x 4.4cm 11 x 7.4cm 12.1 x 4.4cm 12.1 x 4.4cmsize required
Technical Specifications:
23www.alphalabs.co.uk
Electrophoresis Systems
24 www.alphalabs.co.uk
Maxi Power Supplies� High performance,
easy to operate
� Graphic and textdisplays
� Programme constantvoltage and current
� Memory mode –Stores 16 files to recall and re-run
� Safety – Comply to all relevant safety standards
The Clarit-E Maxi Power Supplies can handle virtually anyapplication from high voltage electrophoresis to high currentblotting. Two models provide versatility, offering maximumoutput capability from 300V to 500V, 600mA to 3A and 200to 300 watts. These units are suitable for running multipleSDS gels, blotting and large submarine gels.
Fully Programmable: parameter entry is via the membranekeypad and the start key. Programming includes recall and runconstant or multi-step electrophoresis in a variety ofoperational modes:
1. Constant operation with timer - programme constantvoltage, current or wattage with timer to eliminate thepossibility of over running samples in gels.
2. Memory mode – Store 16 files to recall and re-run favouritemulti step programmes.
Display: The large, 128 x 64 dot matrix display screen allowsboth graphic and text visualisation. Set parameters will bedisplayed continuously compared with actual run conditionsat the touch of a button.
Safety: The alarm will sound in the event of ground leakage,no load or overload.
Catalogue DescriptionNumber
EL2740 Clarit-E Maxi Power Supply, 500V, 800mA, 300W
EL2750 Clarit-E Maxi Power Supply, 300V, 3000mA, 300W
Maxi Power Supplies
Ordering Information:
Midi Power Supplies
� Programme constant voltage and current
� Alarm can be set at the end of the programme
� Auto cut-off function
� Current up to 700mA
These inexpensive, compact Clarit-E Midi power supplies areideal for routine horizontal and mini vertical electrophoresis.Microprocessor control allows user programming, of up to tenprograms, and can be set-up to run in constant voltage andconstant current modes. Four gel chambers can be connectedat any one time. An optional timer can be set to end the runand sound an alarm. Safety features include shrouded plugsand sockets, no load detection and ground leakage detection.
Catalogue DescriptionNumber
EL2720 Clarit-E Midi Power Supply, 250V, 700mA, 150W
EL2730 Clarit-E Midi Power Supply, 300V, 700mA, 150W
Midi Power Supplies
Ordering Information:
Maxi EL2740 EL2750
Max. Voltage 500V 300V
Max. Current 800mA 3A
Max. Watt 300W 300W
Output Type Constant Voltages ; Constant Current ; or Constant Watt
Program Storage 16 programmed files
Program Multi-Step Up to 6 steps
Timer 1-999 mins
Display 28 x 64 dot matrix display screen
Safety No load ; Leakage and Overload Detection
Dimensions 18 (W) x 28 (L) x 14cm (H)
Operation Voltages 110/220V
Technical Specifications:
Midi EL2720 EL2730
Max. Voltage 250V 300V
Max. Current 700mA 700mA
Max. Watt 150W 150W
Output Type Constant Voltage or Current
Program Storage 10 methods 10 methods
Timer 1-999 mins 1-999 mins
Dimensions 16 (W) x 14 (L) x 7cm (H)
Operation Voltages 110/220V
Technical Specifications:
Electrophoresis Systems
Mini Power Supplies� Compact size
� Safety design;shrouded plugand socket; noload detection
� Constant voltage
� Low cost andeconomical
Clarit-E Mini power supplies have been developed specificallyfor horizontal gel electrophoresis systems. These ultracompact power units with dual outlets, are recognised asamongst the most compact and inexpensive power suppliescurrently available and are designed and constructed to themost rigorous safety standards.
Catalogue DescriptionNumber
EL2700 Clarit-E Power Supply, 150V, 600mA, 60W
EL2710 Clarit-E Power Supply, 300V, 400mA, 60W
Mini Power Supplies
Ordering Information:
Mini EL2700 EL2710
Max. Voltage 150V 300V
Max. Current 600mA 400mA
Max. Watt 60W 60W
Output Type 25; 50; 75; 100; 125; 150V 50; 100; 150; 200; 250; 300V
Safety No load detection
Dimensions 10.5 (W) x 15 (L) x 7.5cm (H)
Operation Voltages 110/220V
Technical Specifications:
Catalogue DescriptionNumber
EL2701 Power supply adapters, 4mm to 4mm
EL2702 Power supply adapters, 4mm to 2mm
EL2703 Power supply adapters, 2mm to 4mm
Accessories for Power Supplies
25
Gel Drying
DryerWith a drying area of 21 x 31cm, the Midi gel dryer can drysix 10 x 10cm gels or a singlelarger gel. The Maxi Gel Dryerwith a 35 x 45cm drying areacan dry twelve 10 x 10cmmini gels simultaneously. The unit’s microprocessor controlstemperature and time, each parameter being displayed on itsown LED display. The gels are heated from the base platewhile the vacuum removes the moisture from below to drythe gel homogeneously. These dryers feature optimal sealingusing a silicone rubber cover and supporting mask. Whenapplying the vacuum, a groove that frames the drying surfaceprovides an optimal tight seal during the drying.
Catalogue DescriptionNumber
EL8800 Midi Gel Dryer 220V, 21 x 31cm
EL8810 Maxi Gel Dryer 220V, 35 x 45cm
EL8815 Gelmaster Vacuum Pump
EL8816 Replacement Trapping fluid for Gelmaster
EL8817 Replacement flushing fluid for Gelmaster
Gel Drying
Ordering Information:
Dryers
Temperature Increment 0.1°C
Temperature Calibration Yes
Temp Uniformity ± 0.2°C
Timer 1-999 mins
Drying area Midi 21 x 31cm, Maxi 35 x 45cm
Operating Temp. Range Ambient to 90°C
Dimension W x L x H Midi 30 x 36 x 8cm, Maxi 44 x 50 x 8cm
Pump
Condenser vapour capacity 1.5L
Dimensions W x L x H 35.5 x 28.5 x 39cm
Technical Specifications:
Vacuum Pump� Two pumps in one –
vapour trap and highefficiency diaphragmpump
� Environmentallyfriendly – traps andcontains vapours
The GelMaster™ VacuumPump features two pumpsin one package: a roomtemperature vapour trap acts as a pump by condensing thehot vapours from the gel dryer and trapping them from therest of the systems; this is followed by a high efficiencydiaphragm pump, delivering a powerful, non-fluctuatingvacuum for fast, smooth drying, which acts as the primaryvacuum source. The pump is so efficient, one GelMaster™ canbe connected to three gel dryers simultaneously. Its 1.5Lcapacity allows between 10-15 gels to be dried before thetrap needs to be drained. The GelMaster™ trapping mediumcan be used repeatedly.
www.alphalabs.co.uk
Electrophoresis Systems
26 www.alphalabs.co.uk
Gel Documentation System� 14.7 mega pixel digital
camera
� Image is viewed from a 8"TFT colour monitor
� Lightweight compact hoodwith easy access door andinner built-in lights
� Safety door switch
� Includes flash card, flashcard reader and Ethidiumbromide filter
Catalogue DescriptionNumber
EL4000 Clarit-E gel doc system, including 12 mega pixel digital camera, camera adaptor,5.7” TFT colour screen, ethidium bromide filter, camera hood.
EL4020 Clarit-E gel doc system with UV Transilluminator, 254nm
EL4010 Clarit-E gel doc system with UV Transilluminator, 312nm
EL4030 Clarit-E gel doc system with UV Transilluminator, 365nm
EL4040 Clarit-E gel doc system with UV Transilluminator, 254/312nm
EL4050 Clarit-E gel doc system with UV Transilluminator, 254/365nm
EL4080 Ethidium Bromide filter
EL4090 SyBr Green Filter
EL4070 Thermal Printer
EL4075 Replacement paper for thermal printer.
EL4100 UV to white light Transilluminator screen converter
EL4110 White light box for Clarit-E Gel Doc
Gel Documentation System
Ordering Information:
Camera: Type 5x optical / 4x digital zoom camera
Effective Pixels 14.7 megapixels
CCD 1/1.7" high-density CCD
Maximum Aperture f/2.8 (W) ~ f/4.5 (T)
Shutter Speed 15 ~ 1/4000s
Filters +3 Close up and EtBr; optional SYBR green
Storage Media 2MB memory card
Computer Interface USB 2.0 Hi Speed (mini-B jack)
Video Out NTSC/PAL
Darkroom: Multi-power Source For camera, inner white LED, TFT screen
Inner White Light 2x3 W LED
Safety Device Safety door switch
Side Access None
Weight & Dims 6.1kg; 290 x 220 x 320mm
Voltage Rating 110~220V
Screen: Type 8"TFT
Resolution 600 x 800 Pixels
Brightness 350 cd/mm2
Constant Ratio 300 : 1
Display Mode NTSC / PAL / SECAM mode auto switching
Technical Specifications:
The Clarit-E Gel Documentation system is simple, inexpensive andultra compact. It contains a 16 bit CCD digital camera with asuperb resolution of 14.7 mega pixels. For added convenience,limited space and budget requirements, it can be used computer-free. A variety of images can be captured from agarose andother fluorescent gels, colorimetric gels, autoradiography filmand blotting membranes. The system is fitted with a 55mmethidium bromide filter and has a safety switch to turn off theUV transilluminator when the door is opened. Files can be savedin a variety of formats for transfer to computer systems forstorage and analysis. The Clarit-E Gel Documentation system canbe directly connected to a thermal printer - EL4070 - or to manyother commercially available printers.
Gel Cutting TipsA great new tool for Gene Cloning
Rapid, precise and contamination free excisionof spots and bands from gels
� Eliminates scalpel damage to transilluminator or gel tray
� Designed for removing bands from agarose andprotein gels
� Easy gel slice transfer tips fit standard 1000µl pipettors
� Autoclavable
� DNase and RNase free
� Sterile racked versions are also available
Catalogue DescriptionNumber
EL2650 Gel cutting tips 4mm x 1mm 250 / bag
EL2650R Gel cutting tips 4mm x 1mm 5x rack of 48
EL2660 Gel cutting tips 6.5mm x 1mm 250 / bag
EL2660R Gel cutting tips 6.5mm x 1mm 5x rack of 48
Gel Cutting Tips
Ordering Information:
Gel cutting tips, newly available fromAlpha Labs. No more scalpelsrequired, these tips offer a unique andconvenient way to excise bands fromgels. Gel cutting tips allow completesingle handed and contamination freeremoval of the gel band of interest.The gel band is neatly extracted andcontained in the tip. It can then besimply dispensed into a tube and theused tip ejected and disposed of.
Electrophoresis Systems
Transilluminators
� Three wavelength options: 254/312/365 nm – two Dualwavelength models
� Long life filter
� High efficiency reflector
� Hi/Lo intensity switch
� Fast start up
These UV Transilluminators are multitasking workstations,which offer an ultra-violet source for the analysis offluorescently stained DNA, RNA, and Protein electrophoresisgels. These also offer space to place tube racks, cutting toolsor waste agarose gel, ideal when there is a need to cut bands.
These new generation UV transilluminators include a speciallydesigned safety UV leakage indicator which changes colourwhen UV light is detected. A new and high quality starterensures that the UV tubes energise immediately and withoutflickering. This feature extends the life of the tubes.
Catalogue DescriptionNumber
EL4150 UV Transilluminator small, 21 x 21cm, 254nm
EL4160 UV Transilluminator small, 21 x 21cm, 312nm
EL4170 UV Transilluminator small, 21 x 21cm, 365nm
EL4200 UV Transilluminator small, 21 x 21cm, 254/365nm
EL4210 UV Transilluminator small, 21 x 21cm, 254/312nm
Transilluminators
Ordering Information:
Filter size 20 x 20 cm
Light source: 8W x 6 tubes
UV resistant plastic cover: 33 x 25cm (W x D)
Unit Dimensions W x L x H 34 x 29.5 x 10cm
Technical Specifications:
27
Electrophoresis Reagents
As new techniques emerge in molecular biology so does thespectrum of products available to meet the growing demandsof researchers. Agarose gel electrophoresis, introduced in thelate 1970’s now has a variety of different agarose types, eachof which is optimally specified for particular applications.
Agarose Universal
Agarose Extra
Agarose Reclaim
Agarose Micro Plus
An
alyt
ical
Sep
arat
ion
>10
00b
p
An
alyt
ical
Sep
arat
ion
<10
00b
p
PFG
E
AD
N T
ypin
g
Blo
ttin
g
�
�
�
�
�
� �
�
–
�– – –
–
–
–
–
–
––
� Suitable – Not Suitable
Whatever your performance requirements we are confidentthat we can offer a suitable grade of agarose to suit yourneeds. There are 3 main categories of agarose available:
Routine Agarose – Good general separation characteristicsat a reasonable price. (Agarose Universal).
Low Melt Agarose – Good separation characteristicscoupled with low melt temperatures allowing easy recovery ofbio-molecules (Agarose Reclaim).
High Resolving Agarose – Offering the best resolvingcharacteristics, allowing comparison with toxic acrylamidewhen looking for detailed resolution. (Agarose Extra andAgarose Micro Plus).
www.alphalabs.co.uk
28 www.alphalabs.co.uk
Separation Ranges for Different Buffers with Application Guide
Nucleic acid analytical and preparativeelectrophoresisBlottingProtein electrophoresis such as radialimmunodiffusion
Conventional electrophoresisPulsed field gel electrophoresisBlottingAgarose bead preparationCell and enzyme immobilisation
Fragment separation in analytical and preparativeelectrophoresisTissue and cell cultureViral plaque assays
For applications that require efficient separationof small DNA fragments and PCR products
Agarose 1x TAE buffer range (bp) Gel concentration (%) 1x TBE buffer range (bp)
Universal 20,000 – 1,000 0.6 15,000 – 500
12,000 – 500 0.8 10,000 – 500
8,000 – 300 1.0 7,000 – 250
6,000 – 200 1.2 5,000 – 200
3,500 – 100 1.5 3,000 – 100
2,000 – 50 2.0 2,000 – 50
Extra 40,000 – 3,000 0.3 20,000 – 2,000
22,000 – 2,000 0.5 12,000 – 1,500
15,000 – 1,000 0.8 9,000 – 1,000
10,000 – 400 1.0 6,000 – 500
5,000 – 200 1.8 3,000 – 200
Reclaim 20,000 – 500 0.75 12,000 – 500
16,000 – 300 1.0 8,000 – 300
10,000 – 250 1.25 4,000 – 200
5,000 – 200 1.5 3,000 – 150
2,500 – 100 1.75 2,000 – 100
1,500 – 50 2.0 1,000 - 50
Micro Plus 1,500 – 500 2.0 1,200 – 400
1,200 – 300 3.0 800 – 100
600 – 150 4.0 500 – 50
250 – 30 5.0 250 – 20
Agarose Specifications at a Glance
Catalogue Description PackNumber Size
EL0014 Agarose Universal 100g
EL0015 Agarose Universal 500g
EL0018 Agarose Extra 100g
EL0020 Agarose Reclaim 100g
EL0024 Agarose Micro Plus 25g
EL0025 Agarose Micro Plus 100g
Agarose
Ordering Information:
Agarose Universal Agarose Extra Agarose Reclaim Agarose Micro Plus
Moisture � 7% < 7% � 7% � 7%
Ash � 4% < 0.25% � 0.4% � 0.35%
EEO 0.05-0.13% < 0.12 � 0.12 � 0.12
Sulphate < 0.1% < 0.12% � 0.12% � 0.11%
Clarity (NTU) � 4 <4 � 4 � 5
Gel Strength 1% (g/cm2) � 1200 > 1800 - � 4200 (4%)
Gel Strength 1.5% (g/cm2) � 2500 > 3200 � 500 � 2000
Gelling Temperature (°C) 36 ± 1.5 36 + 1.5 24 - 28 � 40.5
Melting Temperature (°C) 88 ± 1.5 88 + 1.5 � 65.5 � 93
DNase/RNase activity None detected None detected None detected None detected
DNA resolution Finely resolved Finely resolved Finely resolved Finely resolved
Gel background Very low Very low Very low Very low
Electrophoresis Systems
Tris-Native Tris-SDS Denaturing Description
Long 10x10cm Short 10x8.2cm Long 10x10cm Short 10x8.2cm MW Range (kDa) % Acrylamide No. of Wells
EL8101 EL8501 EL8310 EL8709 30-200 10% 10
EL8103 EL8503 EL8312 EL8711 20-200 12% 10
EL8105 EL8505 EL8301 EL8701 30-400 4-12% 10
EL8107 EL8507 EL8303 EL8703 10-250 8-16% 10
EL8109 EL8509 EL8306 EL8705 5-300 4-20% 10
EL8111 EL8511 EL8308 EL8707 5-150 10-20% 10
Also available with 17 Wells. Please enquire.
Acrylamide Precast Gels
Ordering Information:
Matrix: Acrylamide: Bis-acrylamide (30:0.9)
Cassette Sizes (W x H): 10 x 10cm & 10 x 8.2cm
Gel Separation Length: 83mm & 65mm
Gel Thickness: 1mm
Available Comb Configurations: 10 & 17 Wells
Max. Voltage: 250V
Storage: 4°C
Shelf-Life: 12 months from date of manufacture
Technical Specifications:
29
Acrylamide Precast Gels� Compatibility – Long 10 x 10cm and Short 10 x 8.2cm
(WxH) formats guarantee compatibility with all majorbrands of mini gel electrophoresis systems currently onthe market
� User Friendly – the gel is used directly within therunning module with no tape or comb to remove
� Rapid Loading – each gel is lightly coloured allowingthe user to load the entire sample each and every timeinto each well
� Traceable – a unique identification code (UIC) allowseach gel to be easily identified, even after staining
� Long Shelf-life – 1 year at 4°C
Clarit-E precast polyacrylamide gels are ideal for theseparation of proteins and nucleic acids. They areexceedingly user friendly and may be handled directly, withno comb or tape to remove. Each well is clearly visible foreasy loading and can accommodate a maximum samplevolume of 35µl. Unlike other brands, no special tools areneeded to access the gel following electrophoresis, as eachgel cassette is opened quickly and easily by hand.
www.alphalabs.co.uk
Electrophoresis Systems
Please Note: Precast Acrylamide Gels are only available to our UK based customers.
Acrylamide Gel Specifications:
www.alphalabs.co.uk30
Buffers
In electrophoresis a wide range of buffers and reagentsare required in addition to the high quality gel media nowavailable. These reagents must be of at least equal qualityto the separation medium in use. Alpha Laboratories canoffer a select range of convenience buffers. Withouthaving to weigh, mix and adjust pH, convenienceproducts avoid the simple errors, which can mean the lossof complete experiments due to the tedious nature ofpreparing routine solutions.
The specifications offer a high degree of experimental securityin laboratory work across a wide range of scientific disciplines.This means they satisfy the exacting demands of modernscience and are ideal for use in electrophoresis. Purposefullydeveloped and specified for these particular applications. Theyguarantee the kind of results expected with high qualitychemicals.
All these features mean these products are essential for themodern laboratory.
� Save time
� Reduce opportunities for error
� Allow high quality staff to be used more effectively
� Guaranteed consistent reagents
� All batches tested and validated prior to use
� Reduce exposure to hazardous materials
The products are supplied as concentrates for simple dilution.
TAE/TBE BuffersThese buffers are widely used in the preparation of nucleicacid electrophoresis gels. All convenience buffers areformulated with 18 Megohm Water and 0.2 Micron Filtration.
TAE Buffer (50x) is a concentrated solution of 2M Tris-Acetateand 100mM Sodium EDTA in distilled/deionised water (pH 8.3at 1x concentration).
TBE Buffer is available as a 10x concentrated buffer solution ofTris-Borate-EDTA in distilled/deionised water, containing0.089M Tris base, 0.089M boric acid (pH8.3) and 2mMNa2EDTA.
Catalogue Description PackNumber Size
EL0077 TAE Buffer 50x conc. 1L
EL0079 TBE Buffer 10x conc. 1L
EL0080 TBE Buffer 10x conc. 4L
TAE/TBE Buffers
TE BufferTE Buffer is a 100x concentrated solution of 1 M Tris-HCl,pH8, with 100mM EDTA. Manufactured from ultra purereagents, this buffer is the standard for DNA and RNApurification, processing and storage.
Catalogue Description PackNumber Size
EL0081 TE Buffer 100x conc. 25ml
TE Buffer
SDS SolutionAlpha Laboratories SDS solution (20%) is formulated using thehighest quality SDS, that has been further purified to removecontaminants, which can interfere with spectro-photometricanalysis and cause nonspecific binding. The purity of thisproduct ensures that reproducible protein molecular weightmeasurements can be made with confidence, and optimizesactivity recovery in enzyme purification procedures. Theconvenient pre-prepared solution eliminates the discomfortand hazards of working with powdered SDS.
Catalogue Description PackNumber Size
EL0094 SDS Solution 20% (w/v) 100ml
SDS Solution
Tracking Dyes for ElectrophoresisTracking dyes are routinely used in both protein and nucleicacid electrophoresis to monitor the progress of electrophoresisand indicate at what point the gel should be stopped.Generally they are added to the sample prior to loading on tothe gel (loading buffer). Alpha Laboratories offers aconvenient ready-made DNA loading buffer.
Triple Loading BufferWith three tracking dyes (Bromophenol Blue, Xylene Cyanol,and Orange G) Triple Dye Loading Buffer (6x) is a non-denaturing loading buffer for native polyacrylamide andagarose gel applications. In normal type agaroses in 0.5x TBEthe dye mobility is broadly unaffected over the range of 0.5to 1.4% w/v.
The dyes run as follows (linear double stranded DNA);
Orange G 50bp
Bromophenol blue 300bp
Xylene cyanol 4kbp
Triple dye loading buffer contains 50% (w/v) sucrose and40mM Tris base in deionized, distilled water.
Catalogue Description PackNumber Size
EL0084 Triple Loading Buffer 6x 1.2ml
Triple Loading Buffer
Electrophoresis Systems
Ultra Pure ElectrophoresisReagents
Here Alpha Laboratories details a range of reagentscommonly used in the preparation of electrophoresis andblotting buffers and solutions. These are all high purityreagents specifically formulated to be suitable forelectrophoresis applications.
SDS – ULTRA PURE(Sodium-Dodecyl Sulphate)Used as a powerful protein denaturant and solubilisingagent, which induces conformational changes in proteins atlow concentrations, enabling proteins to be separated onthe basis of their molecular weight. The purity of theseproducts ensures that reproducible protein molecular weightmeasurements can be made with confidence. Purified toremove coloured contaminants that may interfere withspectrophotometric analysis. Zero free sulphates eliminatenon-specific binding.
Specifications:
Molecular Weight 288.38
Purity � 99%
OD280 (3% Solution) < 0.1
C12 Sulphate � 99%
Lead (Pb) < 0.0002%
Phosphate (PO4) < 0.0001%
Catalogue Description PackNumber Size
EL0070 Sodium Dodecyl Sulphate 1kg
SDS – ULTRA PURE (Sodium-Dodecyl Sulphate)
Tris Buffer (Base)Tris(hydroxymethyl)aminomethane. Buffer salt used in bothprotein and DNA electrophoresis. Purified to removeammonia and amine contaminants. Results in greaterresolution during isoelectric focusing.
Specifications:
Molecular Weight 121.14
Purity � 99.9%
Pb < 1 ppm
OD280(1M solution) � 0.15
Mg < 1 ppm
Fe < 1 ppm
As < 5 ppm
Cu < 1 ppm
Catalogue Description PackNumber Size
EL0086 Tris Buffer (Base) 1kg
Tris Buffer (Base)
Tween 20 Surfactant – ULTRA PUREA Non-ionic surfactant, used for Western blotting and othermolecular biology and biochemistry applications. Tween 20.Specially purified to remove epoxidized contaminants.
Tween-20 is a Registered Trademark of ICI America.
Catalogue Description PackNumber Size
EL0091 Tween 20 Surfactant 1L
Tween 20 Surfactant – ULTRA PURE
31www.alphalabs.co.uk
Electrophoresis Systems
DNA Markers
100bp and 1kb LaddersTwo-Ready-to-use DNA Size Markers for Gel Electrophoresis.
The 100bp ladder and 1kb ladder represent the two mostpopular markers used for DNA fragment size determination.These markers are formulated to run accurately and toprovide crisp band patterns. The two markers require noadditional preparation and contain bromophenol blue forease of use. Both markers are supplied at a concentration of50µg/500µl (0.1µg/µl).
Catalogue Description PackNumber Size
EL3000 100bp Ladder, 100-2,000bp each
EL3010 1kb Ladder, 300-10,000bp each
DNA Marker Ladder
Alpha Laboratories Limited40 Parham Drive, Eastleigh, HampshireSO50 4NU, United Kingdom
www.alphalabs.co.uk
Distributed by:
International Sales and Enquiries:Tel: +44 23 8048 7209Fax: +44 23 8064 3701Email: [email protected]
UK Sales and Enquiries:Tel: 023 8048 3000Fax: 023 8064 3701Email: [email protected] Ordering Hotline: 0800 387732
Electrophoresis Systems