Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon...
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Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007
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Transcript of Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon...
Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum
tuberosum)
Shannon Engelman 2007
University of MNDepartment of Horticulture
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2,4-dichlorophenoxyacetic acid (2,4-D)
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Background
• Light intensity and hue angle decreases (Rosen, et al.)
Components of Color
• Light intensity • Chroma (saturation)• Hue angle
www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf
Components of Color
www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf
• Light intensity • Chroma (saturation)• Hue angle
Components of Color
www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf
• Light intensity • Chroma (saturation)• Hue angle
Components of Color
www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf
• Light intensity • Chroma (saturation)• Hue angle
Background
• Light intensity and hue angle decreases (Rosen, et al.)
• Coloration affects sales (Fults, et al.)
Background
• Light intensity and hue angle decreases (Rosen, et al.)
• Coloration affects sales (Fults, et al.)• 2,4-D harmful, LD50 of 1500 ppm (MSDS found at
http://fscimage.fishersci.com/msds/96919.htm)
Goal
• To determine visual color, anthocyanin accumulation, and UFGT accumulation differences
Anthocyanin Biosynthetic Pathway
LDOX UFGTleucocyanidinanthocyanidinanthocyanin 3-glucoside
(Keifenheim, et al.)
Hypotheses
With 2,4-D application:
• Increase in UFGT accumulation
Hypotheses
With 2,4-D application:
• Increase in UFGT accumulation
• Change in color (Rosen, et al.)
Hypotheses
With 2,4-D application:
• Increase in UFGT accumulation
• Change in color (Rosen, et al.)
• Increase in anthocyanin accumulation
Methods
Colorimetry
http://www.recreonics.com/images3/pocket_colorimeter.gif
Components of Color
• Light intensity • Chroma (saturation)• Hue angle
www.xrite.com/documents/literature/en/L11-029_color_guide_en.pdf
Anthocyanin Accumulation
Quantifying UFGT
RNA
Quantifying UFGT
cDNARNAreverse transcription
Quantifying UFGT
cDNA Amplified UFGT
RNAreverse transcription qPCR
Results
S S
Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation
L C H A L C H A
Control 47 28.1 12.1 0.24 51 25.7 8.1 0.17 Treatment 45 30.5 7.4 0.52 45 28.8 0.1 0.18 Analysis of Variance p-value <.01 <.01 <.01 <.05 <.01 >.05 <.01 >.05
Field Study Greenhouse Study
Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation
L C H A L C H A
Control 47 28.1 12.1 0.24 51 25.7 8.1 0.17 Treatment 45 30.5 7.4 0.52 45 28.8 0.1 0.18 Analysis of Variance p-value <.01 <.01 <.01 <.05 <.01 >.05 <.01 >.05
Field Study Greenhouse Study
Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation
L C H A L C H A
Control 47 28.1 12.1 0.24 51 25.7 8.1 0.17 Treatment 45 30.5 7.4 0.52 45 28.8 0.1 0.18 Analysis of Variance p-value <.01 <.01 <.01 <.05 <.01 >.05 <.01 >.05
Field Study Greenhouse Study
Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation
L C H A L C H A
Control 47 28.1 12.1 0.24 51 25.7 8.1 0.17 Treatment 45 30.5 7.4 0.52 45 28.8 0.1 0.18 Analysis of Variance p-value <.01 <.01 <.01 <.05 <.01 >.05 <.01 >.05
Field Study Greenhouse Study
Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation
L C H A L C H A
Control 47 28.1 12.1 0.24 51 25.7 8.1 0.17 Treatment 45 30.5 7.4 0.52 45 28.8 0.1 0.18 Analysis of Variance p-value <.01 <.01 <.01 <.05 <.01 >.05 <.01 >.05
Field Study Greenhouse Study
UFGT Field Data
0
0.05
0.1
0.15
0.2
0.25
0.3
1
Treatment
Transcript Level Relative to Actin
Control
+2,4-D
UFGT Greenhouse Data
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
1
Treatment
Transcript Level Relative to Actin
Control
+2,4-D
Conclusion
• Visual color significantly changed (light intensity, hue angle)
Conclusion
• Visual color significantly changed (light intensity, hue angle)
• Anthocyanin accumulation unchanged
Conclusion
• Visual color significantly changed (light intensity, hue angle)
• Anthocyanin accumulation unchanged
• UFGT accumulation decreased
Future Work
• Look at petunidin (purple) vs. pelargonidin (red) anthocyanins
Acknowledgements• Dr. Cindy Tong• Harpartap Mann• Dr. Carl Rosen
• Ms. Fruen• Team Research
Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum
tuberosum)
Shannon Engelman 2007
