ECDC — a totally integrated and interactively usable genetic map of Escherichia coli K 12

Microbiol. Res. (1995) 150, 7 - 61 Microbiological Research

© Gustav Fischer Verlag Jena

ECDC - a totally integrated and interactively usable genetic map of Escherichia coli K 12

Ralf Wahl, Manfred Kroger

Institut fUr Mikrobiologie und Molekularbiologie, Justus-Liebig-Universitiit GieBen, Fachbereich Biologie, Frankfurter StraBe 107, D-35392 GieBen, Germany

Abstract

A printed version of the interactively usable genetic map of Escherichia coli K 12 is provided together with some statistical information about the actual status of the respective genome sequencing project. A total of 3179967 bp corresponding to 68,38% of the genome is available through the ECDC database. Contigs as well as individual DNA sequences for each gene or open reading frame are provided. Access to a number of other databases is possible using World Wide Web or local programs.

Key words: Escherichia coli - genome sequence - database collection

Genome sequencing seems to become easier each year (Oliver 1994). Several internationally cooperating projects are already launched in order to systematically sequence entire genomes of selected model organisms, e.g. Bacillus subtilis, Saccharomyces cerevisiae (yeast), Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, and Homo sapiens. However, the most widely accepted model organism is still Escherichia coli K 12, which has not been object of an internationally organized genome sequencing effort (Swinbanks 1994). Instead, since 1967 an almost uncountable number of individuals has published pieces of sequence information with various length. Only the group ofF. Blattner at Madison, WI has performed systematic sequencing of E. coli. The first report of this group appeared in 1992, followed by six others (Daniels et al. 1992; Burland et al. 1993, 1994; Plunkett III et al. 1993, 1994; Blattner et al. 1993; Sofia et al. 1994). A group from Japan, headed by K. Mizobuchi and K. Isono, has started a program to fill the gaps between already published

Corresponding author: M. Kroger

sequences covering the region between min 0 and min 33. This group has released two reports since 1992 (Yura et al. 1992; Fujita et al. 1994). The group of G. Church, Boston MA, has started a program to test the capability of multiplex sequencing on E. coli K 12. The first two reports are available (Richterich et al. 1993a, b).

At the beginning of the year 1995 a total of 3 179967 bp is available. This corresponds to 68,36 % of the entire E. coli genome, expected to be 4 762 000 bp in length. According to our statistics one may expect that the entire sequence will be finished by 1997 (compare Table 1). Presentation of these data, however, exceeds already the limits of space of any type of hard copy. Many journals already stopped to print DNA sequences and many authors started to publish their data in electronic form only (e.g. Richterich et al. 1993 a, b). On the other hand, there is still a considerable demand for hard copies of sequence information. The scope of this

Table 1. Time scale for the growing of the E. coli DNAsequence information. At January 5th, 1995 ECDC consists of 368 individual contigs, derived from 1.912 sequence entries. The total number of nucleotides is 5302213 bp, which corresponds to 114%. This number exceeds the actual size of the E. coli genome considerably and reflects the fact, that large parts of the sequence have been determined in more than one laboratory.

date actual information percentage

January 1989 940449 bp 19.92% February 1990 1248696 bp 26.46% February 1991 (492282 bp 31.62% February 1992 1820237 bp 38.56% April 1993 2353635 bp 49.87% June 1994 2878364 bp 60.98% December 1994 3179967 bp 68.36%

Microbiol. Res. 150 (1995) 1 7

paper is to provide a compromise between a full length DNA sequence hard copy and a simple genetic map. The map given in the appendix is both, a very detailed genetic map and an electronic interface for information retrieval either via INTERNET or local programs on personal computers with MS-Windows (for details see below).

Previous and supporting efforts

In 1983 we started to collect the DNA sequence data of Escherichia coli, mainly for K 12 but also for all other strains. Our collection is based on database entries as well as on our own literature searches. We first reported about our database in 1989 as part of the annual Nucleic Acids Research database issue (Kroger 1989; Krogeret al. 1990,1991,1992,1993; Wahl et al. 1994). Our database is available on the CD-ROM of the EMBL data library since 1991. Because of the extraordinary interest in these data, E. coli sequences have been collected independently also in three other groups. Most recent papers are: Rudd 1992; M6digue et al. 1991, 1993; Kunisawa et al. 1990.

Since the beginning we collect the E. coli DNA sequence data and include as many additional information as possible. Gradually it became clear that neither the presentation of DNA sequence nor of any additional data can be performed in any other way than in electronic form. In the same period of time computer handling became easier and less expensive. Thus, the idea has been developed to connect the entire knowledge of E. coli K 12 in a single database system. Consequently, we created ECDC - the Escherichia coli database collection, which is presented here.

Content of database collection

A part of the enormous interest in the E. coli genomic DNA sequence comes from the fact that about thousand different genes are functionally described for E. coli K 12 (Bachmann 1990; Riley 1993; Selkov 1994). A well accepted genetic map has been maintained for several decades by B. Bachmann at New Haven (Bachmann 1990). Genetic informations and the respective mutant strains are collected at the New Haven E. coli Genetic Stock Center. Contact M. B. Berlyn for CGSC database at New Haven (Berlyn and Letovsky 1992). A detailed physical map has been developed by Kohara and Isono (Kohara et al. 1987). According to different DNA sequences and to the physical map, the genetic map has been recalculated. The new data are given in Table 2. A number of refined functional analyses of the sequence data has been performed, namely on promoter (Lisser and

8 Microbiol. Res. 150 (1995) 1

Table 2. Concordance of physical and genetic map data

Bachmann Physical Bachmann Physical map map map map

0.0 min 0.0 min 55.0 min 57.75 min 5.0 min 4.90 min 60.0 min 62.90 min

10.0 min 10.00 min 65.0 min 67.90 min 15.0 min 14.55 min 70.0 min 72.70 min 20.0 min 20.55 min 75.0 min 76.35 min 25.0 min 25.33 min 80.0 min 80.00 min 30.0 min 30.30 min 85.0 min 85.30 min 35.0 min 35.65 min 90.0 min 90.10 min 40.0 min 40.90 min 95.0 min 94.90 min 45.0 min 46.20 min 100.0 min 100.00 min 50.0 min 52.35 min

Margalit 1993) and terminator structures (d'Aubenton et al. 1992), as well as on ribosomal binding sites (Rudd and Schneider 1992) and on the distribution of REP sequences (Dimri et al. 1992). Other most valuable collections are available which regard tRNAs (S. Steinberg et al. 1993), distribution of insertion elements (Umeda and Othsubo 1989; Birkenbihl and Vielmetter 1989), chromatographic behavior of proteins (VanBoge1en et al. 1992), and metabolic pathways (Riley 1993; Selkov 1994).

Description and structure of ECDC database

Data management and program development was performed using Borland C+ + and MS Foxpro for Windows on personal computers and C on UNIX. The database retrieval software is available for MS Windows and will also be available for Apple Macintosh in the near future.

Starting with ECD release 20, the content and structure has changed completely. Thus, we call our collection ECDC for Escherichia coli database collection. For a principle description ofEeD see Wahl et al. 1994. From now on we are able to align the EMBL entries with the physical map of Kohara (Kohara et al. 1987). Additionally, we use these entries to build sequence contigs and align them with all previously determined sequences, including RNA sequences. The exact genetic and physical map position of each described gene, open reading frame or regulatory region is calculated. Each coding sequence may be extracted directly.

The respective genetic map is presented in the appendix, which may be read using the explanation given in Fig. 1. The map is divided into portions of 0.5 min; four of these portions cover one page. Note, that in the electronic version of ECDC all gene names or contig numbers allow to open a cascade of

1 - 7,50

2 - I.···. _ .. ·1········ ·1········ ·1········ ·1········ ·1········ ·1· 3 - » Q « 4 -5 - ECD007.50 6- > >~

7 - X63656 M23219 V00295 X03527 8- --> -> <- <-9 - 556903 M17890 M24304 V00294

10 - <--> < <-11 - M93053 M17891 V00296 M24298

12 - <------------------13 - J01636 14- <--15 - X51872 X75464 16 -17 -18 - --> <- --> <- <----19 - cOOB cynR cynS lacA lacZ 20 - -> -> <-- <-21 - codA cynT lacY lacl 22 - -> 23 - cynX 24 -25 -26 -27 -28 -29 -30 - ?dciQ

Fig. 1. Organization of the ECOC genetic map. A part of the ECOC map as given in the appendix is used for explanation of the different types of information organized in individual lines. Lines 1 and 2 give the start and end map position of each section together with a ruler. Line 3 denotes each tRNA (T), promoter (» or «, respectively) hairpin loop or terminator (0), and bending site (B). Line 4 denotes the extend of individual contigs. Line 5 gives the respective name. Lines 6 through 17 show location, extension, and direction of individual ONA sequence files, together with the respective EMBL/GenBank AccNr. Lines 18 through 29 describe location, extension, and direction of individual genes. A "" symbol at the end of a name indicates partial characterization of the respective gene. Open reading frames are named according to the SWISS-PROT nomenclature (Bairoch and Boeckmann 1993) or according to a temporarily used internal accession number of ECOC (e.g. E004321 or C001234) (Wahl et al. 1994). Each name represents a single entry in the ECOC database and may be used to find alternative names. Access via an alternative name will always be internally guided to the names shown in the genetic map. Line 30 gives the approximate genetic map position of un sequenced genes according to B. Bachmann (Bachmann 1990).

additional information. Within the Internet version, these cascades guide the user always into the most actual data base entry maintained at various sites all over the world. The maintenance of these individual files is not performed via our program. It is always within the guidance of the original database, exclusively.

Beside a number of 1895 mapped and sequenced genes, 1042 open reading frames are discovered. A small number of sequenced but still unmapped genes has been collected within ECDC as well. The respective data are given in Table 3.

Table 3. Unmapped ONA sequences most likely derived from Escherichia coli K 12

EMBLname

EC2IDAS2 EC3ID1 ECALPHOL ECALPHOP ECALPHOS ECALPHOW ECASR ECBAESR ECCOMS ECCHM ECCIPL ECCOBS ECDIGOXAB ECONAHPCE ECOOGR ECOPR ECFLIA ECFOCCO ECFYUAK23 ECFYUAK49 ECGST ECHPCG ECICC ECMPB161 ECNEUA ECNEUC ECNFNB ECOMPP ECORFl71B ECPYU09 ECPYU15 ECPYU18 ECREGX ECRNEC107 ECSFAAG ECU03846 ECXYLUP1 ZM327

AccNR Contig

017632 ECOllO.OO 017631 ECOllO.OO M33734 EC0116.06 M33738 EC01l6.05 M33741 EC01l6.07 M33745 EC0116.08 L25410 ECOI01.00 014054 ECOI02.00 M87625 EC0113.03 X53666 ECOI04.00 L05182 EC0106.00 L25054 EC0103.1O Z37966 EC0131.00 X75028 ECOll1.00 M81463 EC0115 .03 001031 EC0116.01 L36677 EC0135.00 Z46635 EC0130.00 Z38064 EC0129.00 Z38065 EC0129.00 038497 EC0126.00 X81446 EC0127.00 016557 ECOI09.00 M29860 EC01l3 .01 J05023 EC0114.00 M84026 EC0114.00 025414 EC0125.00 X74278 EC0115.01 X75949 EC0115.04 021147 ECOI00.03 021153 ECOI00.04 021156 EC0100.05 X66589 EC0118 .07 X62583 ECOll3.07 X17420 EC01l9.01 U03846 EC0120.03 X04387 EC0124.01 T15327 ECOI23.00

Length

614 nt 1735 nt

67 nt 116 nt

87 nt 59 nt

1292 nt 2495 nt 2847 nt

532 nt 765 nt

1231 nt 4614 nt 1240 nt

378 nt 201 nt

1124 nt 3436 nt 2319 nt 2318nt

907 nt 807 nt

2360 nt 87 nt

1283 nt 1676 nt

998 nt 2269 nt

780 nt 300 nt 300 nt 300 nt 256 nt

1300 nt 762 nt

1883 nt 363 nt 418 nt

Data distribution in machine readable form

The interactively usable ECDC map is available from the EBI data library and is automatically distributed with each release of the EMBL data library. In addition, it is available on the CD-ROM version of the EMBL data library. The actual version of ECDC may always be found on our anonymous FTP site (susi.bio. uni-giessen.de) and is interactively accessible on World Wide Web; please use WWW URL: http://susi.bio.unigiessen.de/usr/local/www/html/ecdc.html. A specific set of data may also be available on disk or CD-ROM on personal request from GieBen, directly. Please use our email address KROEGER @EMBL-HEIDELBERG.DE or RALFW AHL @MIKRO.BIO.UNI-GIESSEN.DE.

Microbial. Res. 150 (1995) 1 9

0,00

b' [email protected] > X6BB72

J01706

010483

> thrL

thrA

?hrp8

I~:~~.

188666. 66 0104B3 =>

·1· .... · · .. 1·

=> L13601

-> thrB

--> thrC

<yaaA

*tolJ*tolr *popD

I . ·1 ..

J01597

··1··· .. ····1 .... · .. .. 1 .. · .. (l

I·· 0,50

=> ;:::> => > X67700 K0129B M11300 S67239 836154 M16640

=> L03720 010765

=> => M10420 M12544

=> M12565

<= X17311

< X043B2

=> J03B 79

=>

> L34404

> M16641

> M16642

=> L24072 M1042B

Z28369 --> 6004553

-> yaaG

- - > ----> --> --> > --> htpY dnaK IS1B6B nhaA IS1 yaaC

<-- --> < --> <-yaaI dnaJ gef nhaR B004558

<E002992

*gprB *gprA

-> 8004557

<rpsT

iIeS

. . I· .... . . I .. . ...... I ... .. ... ·1· ... •. ... I ....... . . I .... ~: ~~.

<= X6774B Z2B369 X54945

=> K01990 MI061~ =V=0=1=5=0~0======== => X00776

=> <===================================== X70017 X73904

---;> --> --> iIeS yaaD yaaF

--> ;"spA

--> lytB

*chlG

--> dapB

I~~~~ ..... I . . . . . . . I . . .

[email protected] 0104B3

X56742 > <=========

X04711 => <== J01609 M11054

carA

<= X0510B M6B521

carB

-> --> <- <----yabB

--> BG02844

folA apaG surA <--apaH pdxA

----> <--kefC

I 1,50

UcB666.68 010483

M15263 <= J01641 => V00256 => K01303 => V00259 --> araC

-> yabI

ksgA

1 .. ·..1 · ·1·

<= U09984

<- <--sfue sfllA

<--sfuB

*psu

10 Microbiol. Res. 150 (1995) 1

<- <--caiE: caiC

<-caiA

<caiD

<--caiB

<--caiT

-;> -> -> fixA fixE fixx

---> fixe

yabE

*rimG *rimF *mafA

·· · 1· .. ·· .. ··1 ........ ·1· .. · ·1 .. .... .. ·1 .. ·· .... ·1 .. · 1,50

~B1963 ~;=1~5~2~6~3============

X:c5=4~B=4~7=========== ~~4279

~=3=5=3=7=1=============

~:~6~2~6~4~6========== <======= M3B2B3

<--- <-- <---hepA araO araB

<:-:-:---- <---polB araA

*brnS *ilvJ

··1 .... .. .. ·1· .... ····1 .. 1 .. .. .... ·11 .. 2,00

(l

<= => J01642 X01609 X52063

X55034 <= => M12B91 M35034 K00137

=> => -> M21150 X55457 549875

<-- <-- < leuL

---> > --> -> yabB ftsL --> yabC

leuD leuB <--leuC

<--leuA

ilvI fruL --> leuO

*mafB

--> ilvH

--> fruR ftsI

*ftsM

1

2,00 I· ··1 ····1· , ··· ·1···· . I . . . . . . . I . . ··1 · · ··· · ··1··

2,50

828888.66 <=

0104B3 X07917 L20B34 <=====

X55034 L2810S => => => => => => K0013XlS432 X17609 X52540 M14029 M36531 Ml9211

> S5B711

X55814

--> murE

X51584

--> mraY

X52644 => M30B07

---> ftsW

---> murC

X02 B21

-> ftsQ

=> M10429

--> ftsZ

M20791

--> yacA

=> X04831

026562 --> mutT

--> guaC

---> murF murD murG

--> --> ddlB ftsA

-> envA secA

<-hope

*serR*mraA *mraB

2,50

1······1 Q ..• I· · · · · ... · 1· ..... " . I · ·

EeBoBS. 88 D26562

L28105 Xl7333 =>

L20834 X15237 <= L20833

<= M15312 => S67363

V01498

<- --> -> ----> hopB nadC ampB pdhR aceF

<- - - > <--- ------ppdD ampD aroP aceE

*lrs

3,00

I·····

---> lpd

· 1······· · · 1·· · · · ····1···· · · ·· ·1·

J'''''0''''2 ""8:C0 4:=====

-----> <-- <-yacJ speD yacC

<- ---> speB yacK

<-hopB

*prlD

3,00

<= X51323 <= D12651

<--gcd

I· . ····1 · · 1· .. · · ··1···· ·· · 1 ·1 · . . . . . I . · · ·1··· . . [ . . 3,50

[email protected] D26562

X51323

012651

~'=1=7=0=8=6============

<= X65538

~':0~0~6~8~0=---===

gcd --> hpt

3,50

<- - -::;. yadF nodJ

--> nodI

-> yad8

- > < -levD panD

*ssyD

[. . . [ . . ·· 1· I

E~B888. 88 D26S62

<-- <-----pane htrE

<panS

.. [. . . . . . [ . .

=> <= X02163 X05Bl0 X53696

M124B6

X04319

<ecpD

<--

<= L06495

<= S48039

<=<= M20574 M34945

<= X64595

<= M60726

8004545 <-8004546

- - > 8002048

<-dksA

<-

. . [ . . .

<- <--E004134 penB

<- -folK

. . . . .. [. . . .... .. [.

sf sA

. . I . .

=> D42122

-> 8004548

E004549

*garB

4,00

<= M31772 K02970 <= => <= MB3735 X12457 Z21B42

M29955 => M33193

--> --> <--- --> <- ---> <- <-8004549 fhuA fhuO hemL yaclR pfs htrA yaeH dapO -> ----> ---> <-- ---> <--mrcB fhuc fhuB yadQ yadS dgt glnO

*dapC

2

Microbiol. Res. 150 (1995) 1 11

4,00 , ... .. . . , .... . , .. ... ... , ... . .. . . . , . . . . . . . .. , ... .. . .. . , ..... .... , .. ....... ,. 4,5 0

(l

dCB863. 36 026562

Z21842

glnO

V00343 <= M15106 013334

= > X78809

- > - >

=> J05l13

rpsB smbA <-- - - > - -> map tef frr

*envN *tdi '*"eefA *aerC

~.40 !E8661 .45 = > Hl1330

--> edsA

= > X75465 M19334

= > M21118 => X54797

-- > skp

lpxn>

fabZ

4,50 , . . . . ., . . . , , . .... J ... . J ....... J ......... J . .. ...•. . J . . J . . .

5,00

TT

!Mol.45 M19334

> 841344

= > M96394

--:> - > - - > IpxA rnhB aceA ---> ~-----------

lpx8 dnaE

*metD ·fadE

5,00 J ......... J .....•• . . J . . ... .. . . J • • .. .• . . . J . . . . . J .

.cT

[email protected] 01 2 649

rrlH

Q

= > X56780

D12650

~=60=7=3=9===

> rrfH

<-- -> yafe yafll

- > <yafD dniR

< ll003184 --> yafB

1E8l!!!'P.'22 = > K009 85 <= V00337 <= X0402 7 <= 547111

= > X14007

<dnaQ

- -> rnhA

&@88t.36 ~=3=2=35:'7=== <===== M97858

<= L08626

> V00332

= > J01 7 00

> KOO139

=> M20398

================ > 015061

<= L04474

<---- <--proS reeF

<-yaeB

J . .. .. • ... J ..... .. . . J

<- abc

K00217 = > > X67217 MI092 7

= > K02555

012649 --> rrsH

rrlH

5,50 . . 1 . . . .

J· ·· ·· · ···I· ... ·1··· T ... J. ..J. A

. J . . . . " . . . J .... 6,00

R@aSt .kg <==== H34034

<= X14790

M13422 => M12907 => X00221 => X00222

- - >

=> X67207 <= V00316

=XO=07=86==

-> --> prf H gpt crl proA

<------ -----> <-- ---> pepO y afA phoE proB

'*" l pcA

12 Microbiol. Res. 150 (1995) 1

3

6,00 I· ··1 .. · · · ···1· ·· · ··1· · · ···· ·1······ .. ·1· .. ·1·

6,50 1 .. ···1· ·1 · ··· . . . 1 . .

ftC8b6U6 L20943

<= X17345

--> 11004848 ~> --> IS1 B004806

.. ·· · 1· .. ·· . 1 . . 1

*katC

~37 => M24185 => V00260 => X00759

--> argF

*cxm

... .. . I·.···:i:· I ...

~6.88 => 012594

-> eaeH#

. . . . 1 . . . .

7,00 I··· ··· .. 1· ·1 .. ·· ·1 · ·1····· ····1· · ······ · 1·· · ···· ·· 1····· · ···1········· 1···

*strC

7,50

I@66).6; < M77738

~~5=2=9=05~========================

<= M77739

<--betA

<betI

betB betT

*ilvu

-> yahA#

*trmB

1···· .. ···1· .. · ···· ·1· · ·· • • 1 • . • ·1 ·· 1· »

liMb'. 56 X63656

=> 856903

(l

===========> <= < X03527 M23219 V00295

=> <= M17890 M24304 V00294

<==> <============ <== )193053 M17891 V00296 M24298

---> codE

--> codA

<cynR

< J01636 <= X51872

--> cyn8

<lacA

<------lacZ

-> cynT

<--lacY

--> cynX

> X75464

<--lac!

*dvl

·1·· . . . 1 . • • • 1 . .

6,50

7,00

7,50

8,00

4

Microbiol. Res. 150 (1995) 1 13

B,OO I · ··· · · 1· · ··1····· · 1 ·

H&66i .iB > 853042 <= M244BB

<= X17417

<--hemE

B,50 I··· ······ 1····· ··· 1 . . . 1

Q

BcB8od.g; ~8 1&861,6;

· · · 1···· ·· · · ·1 · · .. · · · .. 1·· .. .. ··· 1 .. ·· · .. ·· 1· I

. . . . · 1· . 8,50

Ift!!OOB.42 = > D12595

*brnR

. 1 . . • . . ··1 · .. · ··1 · ········1······· .. 1··· .. .. · ·1··· 9,00

»

~8.71 &&66 •. &2 => <= => ===> <========= <===== X54153 M5B4 67 M1 33 45 J01665 X04064 X76979

=> M13045

X15981

--> abmA

9,00 1 . . . . . ·1

kEddo' .85 Q

= > M647 8 7 X04026

<--ddlA

. . . 1 Q

= > X1 5981 X04704

-> sbcC phoB

<-- ---> abeD pheR

?alsB

=> M29664 = > V00315 = > X0 45B6

::. - - > y aiB# psiF ---> phoA

proe

*brnQ

- - > - - > aroL yaiB

- > <-aroM y aiD

*lev

~~6~4~7~B~7===========

--> ya jP

<~

V002 5B

<-----sbcC

<- -araJ

.. I · .. ·· .. ·1 .... · ···1 .. · ···1 .. ·· .. · · · 1 .. · ··· .. · 1 .. .. · .. ·· 1 9,50

U&SSY.tZ X5 9B39

M37702

= > M63 939

X56175

<= M57685

----> --> - - > --> <--malZ queA y aj C seeF tax

<- --> - ---> -> yajB tgt seeD yaJD

*seaD *nuvA

19

: 50 .. . . . 1 ......... 1 .... . . ... 1 . . . .. .... 1 ......... 1 ... . ..... 1 ......... I. 1· .. .. · .. ·1· 10.00

*xseB

i@6D@ba§ .s6 ===> M23546

<= 000694

pgpA <-ispA

*seyB *thiL

14 Microbial. Res. 150 (1995) 1

» »

12&S;.I; J~O~54~92~================= =>=>

X17642 J05534 J0 3 896 <= = > = > M5525B M34066 Z2327B ~=6~7~B~1~6======= = >

LIB867 <= XB215B = ===

<cyoB

<----eyeS

<- eyoD

<- cyoC

<--eyoA

<= X82159 <= X82160 <--- --> ampG bolA

- > elpP

M38347

L12349

L20572

len ---> --- > tig elpX

*fhiL *leuY *ras ?hs lA

5

10,00

1·· ·· 1 • Q

I· · I ·· · ·· I ~ I . . . . . . I . . . . . ·· ·· 1· ·· · · ··· · 1··· ·· 1· 1 0, 50

""009.698@616 .65 m10. 2l ~26 m10. 40 n~~e!n.'~ > > => = > => = > JOX54554 M266 39 X01074 M63306 X57977 U00734 > M3B347 X64395 M94246

= > L12349 X00661 > = > L20572 U09348

-> - - > -- > - > - -> -> hupB ybaO r ibH ffs tesS hha acrA

--> - > --> ribG nusB ybaA

?hslB ?mac "'tInA

. . I . ···1·· .... ···1· · · ·· .. . . 1· · · ·· ·· · · 1· · ·· · · ·· ·1·· · ·· · ···1 11,00

HeBolo. 46 Mm.60 IIIIdE-"b""ib".g.-g --------

. . . I .

~2 U00734

M9424B

> acrA

acrB

11,00 1 ···1

<= 000727

< - -priC

... · 1· ·

=> = > M14040 M37064 090259 ================= M38777

- > - >

apt _______ ybaB _______ >adk

dnaX htpG -> recR

--> vi sA

L35149 => 00079B

<B002077

-----> gsk

= > X03695

------> ushA

····1· ··· 1········ ·1· ··· · I ····· ·· · 1· · ··· · ···1 · ··· ·····1·

II'@!!!! . 20 !!Eo 0 11 . 2 5 => L061B2 => 0131BO

--> tesA

= > X60997

&&6££ .31 X60999

= > => M21764 M21 76 B

= > M2971B = > M29719

rhsD

11,50

11 , 5 0

I···· ·· ···1 ······ ···1 · · ··· ···· 1· ·1 · 1···· · ····,··· · · · · ·· 1·· · · · · · .. , .. . . . . I . . 12,00

1@6ff .5i a@dii .fl' L03845 L16945

-------> gel nfrB

- > gip nfrA

'g~@-6~1~1·. ·;·1 .. ~97 < M19ti57 <:===== X129B2

<= M55430

X59293

X56234

M593B1

<-- <- -purK ppiB

< - -purB

eyeS

6

Microbiol. Res. 150 (1995) 1 15

12,50 12,00 I· ···1·· · · ····1·· . . I . . "BTl ......... j ......... I · .... . ... I . . . . . . . I ......... I ..... Ii" .

IiMil.97 =><= X5929M74 789 => X56234 => M59381 <= Dl0588

-> <-eysS folD

*ksgC

1i&8B.ii =>

X51662 X02311 <~ => X05962 M62732 => => S65870 ZllB77 <==== M31074

<fimZ

=> D12596

<-int

--> IS3C

--> mvrC

dC66i2.h J02580

<~

X05964 <= JOl735

<~

X05965

< <-PA-2 rz# C0052S0 <-C005249

<--rSSB

--> nmpC

*qsr 1

12,50 I· ······1····· ····1·· . . I . .

13,00

1······ · ··1······ · ··1· · ·· ..... j .. ··•· .. ·1···· ·····1······ ...

~012.52 => D00928

<-nohB#

13,00 I·· ...... ·1 .. ·

n

[email protected]; => <= M24530 X0034B

=> <= Y00138 X1354B ~=0=6=9=0=3==

<= M23630

-> appY

<-ompT

*6UpQ

<envY

···1···· ... ··1· B

*nfnB

1 .... ·· .. 1· .... .... 1· .. . .... 1 ...... .. . 1 .. · ...... 1 13,50

k@61].8£ ~013.10 'iE~8~81~j~.£~5~ ........................................................ .. ~>

M5BOOO X639Bl

---> pheP

13,50

=> X03123

<===== M13748 M60177

<= => Xl7426 J04216

--> <----IS186A fepA

-> 8002439

--> <entD fes

entF

<======<= X57471 M29730

=> X74129 X59402

=> X57470

--> <-- --> ybdA fepB fepG

<--fepC

<-fepD

<-fepE

I····· ····I·· · ····· · J· .. ·· .. ··1· .... I .. · ...... J. 14,00

1 .. ·······1·········1·· .. ·····1 .. · .. ·· ..

LEgBiS.ig =>

M29730M2749Q X52904 => ===== M24142 => M36700 => X12670

M24148

M24143

=> X15058

--> --> --> -> entC entE ybdB ybdC

---> -> ---> - > entE entA cstA ybdD

16 Microbiol. Res. 150 (1995) 1

*!euJ *rimH *dpp

~84 => D13187

-> ahpC

--> ahpF#

1EBm.91 => M556B7 ~>

848361

-> rna

7

... I ... . .. .. . j ......... I ... . ..... I .. ... .. .. j ..•••...• I .. 14,50

I ~~: ~~ ... . j . ... . .... j • ..•• , . , , I·

irenvT

14,50 I ~.. . . · 1

1I'!!!014.29

X04516

< ybeB

D@b!l .;; <,================== <===== <====== L07636 M18276 X04516

<= <= X06479 11228 57

<-- - > <-rIpA

<----lipA B002084

<- -lipB

<--dacA

mrdA <--mrdB

< pbpA

<-B004707

15 ,00 . . I . . . . . .. . . I ...... .. . I .. .. " . .. I ... . ..... j ......... I . . . . . I . . . .

dl66U.tg <= L04576 <= 1194267

<= 11182 77

<===== ==== X06 33 1

<-- <,- ----holA leuS

<-rIpS

dESbi •. g, X5807 0

---> cutE

*fusB '*'ubiF'

15 ,50 15 ,00

I········· j ··· ·· · ···b·· I . . . . . . lo···o···I ... .. .. " j . ... . .... I . . . . . I ......... I .. . «

15,50 I ...... .. I

~O = > U08369

- > pgm

"calc

iMig .IS <= JOl7l3

<========== X14135 V01575

< K00296

< J05554

======

<--- <asnB nagO

1119284

<--nagA

<-- <nagB nagR

?nagC

----> nagE

=> J01617 > M163 68

---> gInS

£var§ .tg <= X02589 <= 1159426

U07651

<--fur

<-fldA

U08369

- > seqA

. . . . I . . . .. · 1·· · ······1 · . .. ... I .... .. . .. I . .. ...... I ..... .. .. I ........ . I .. . 16,00

B

'WBig ,ig <,============= <:================ = > 1164495

<---potS

<-----speF

< 1136066

K02670 1'12176 3

L02373 = > X63979

<- < - <---kdpB kdpC kdpA rhsC

<----- <,-----kdpD kdpB

*garA

:;;;:> = > 1121 765 M21766

- > B004 702

8

Microbial. Res. 150 (1995) 1 17

16,00

1·········1· . . . I . 16,50

·· · 1····· ·· · ·1·· ·.·····1·········1·········1··· ······1· ....... .

HEBgIs.tg L02373

--> 8004703

K01299 => X57399

-> B004705

--> --> 8004704 ybgA

---> phrB

*rdgA *cbt

::t» >::t>::t> >

[email protected] J01619

< => M29373 X01070

=X=0=09=8=0===== =X""00=6""6=1====

<= V01501

<--- -> gltA .dhC

--> sdhD ---> .dhA

-> sdhB

eucA

16,50 I ··1 . . . I . . .

Q . . I .

17,00 ·1···· . . .. ·1········ ·1······· ··1·· '~'il' ··k···· .~ .. I ........ ·

[email protected] Wd~C6~6'lg".'Pg .. ""'" dEBBie. 7. ~94 ~016.99 J01619 > X00661 => X00664

<= X15790

--> --> -> sueS suce sueD

<-ybgB

J03939

---> cydA

--> cydB

=> => > M16489 X05123 X02682 J01591

=======> => ~> M28232 X04171 X12713

=> X65796

-> ---> --> tolR tolA pal -> ---> tolQ tolB

> K00282

--> nadA

?pnuC

17,00 17,50 I········ ·1··· ... ··1········ ·1········ ·1 · ·· .. . . ··1· · · ..... ·1· ....... ·1·· ..... ··1········ .1.0. ....... .

£@[email protected] BY!; .S; => <=< J01591 X06226 M24169

=> > X69182 M1ll51

<= J01613 => X02306

-> <-- --> aroG galT galK

--> <--phrA galE

*phxB ?gpmA

17,50

dEBsr, .52 L34009

=> M16182

--> --> modA mode

--> > modB modD#

"'pgl*eap

dWbi'.t6 => J01638

J04423

<--ybhB <-bioA

1· . . .. I· I . . I . ···1···· · ·· ·1 · · .... ···1·········1·· · ······1·········1···

HCSS!; .tt J04423 > M10280 > X01201

X03722 => 368059

==== X03678

> M29632 > M29633

--> --> bioD bioA bioF

--> --> bioB bioC uvrB

18 Microbiol. Res. 150 (1995) 1

Q@OOI 7 .ig X70420

--> --> moaA moaC

--> moaB

-> moaD

--> moaB

arn8!' .do M22041

---> citA

&@6i7.;5 L02123

=> X56307

---> rhlE

*gurC

<ybiA

9

I~B:~~ .... I .. Q ····1 .. ····· .. 1· .. . .... I ......... I ......... I .. ~ ...... I . ........ I ... . ..... I .... 1B:50

D&B±I .. g a&8±Ug L02123

MBl935

--> <--ybiA ybiB B003 97 7 ----> --> dinG ybiC

< X14180

=> L37088

*fiu

< UO~242

<= X69337

<- <-glnQ glnH 8004101

<- <-- --> glnP dpa ompX

1B,50 1 · .. 1 .. ···· .. ·1··

19,00 ·1·········1· .. ······1 .. · .. · .. ·1 .. · .... ··1 .. · .. .... 1 .. ·· .. · · ·1· .... · ...

UEbb¥i,gg <==== M21151

<chIN

<---chIll

1!!IPl'8 . 81 => M13449

-> grx

19,00 19,50 I···· .. ·· ·1· .. ····· ·1 .. ··· .... I ...... .. . I ....... ~.I ........ ·1· .. ...... 1 .... .. .. ·1· .. · .. ·· ·1· ..

BEBSig.S! => X064BO

<= X02B37

--> dace

<-deoR

AgiUG M93239

--> --> potF potH

--> potG

--> pot!

*opr ?hslC

B!P.'24 => X15859

~bjB# --> rimK

D&6iU£ => X04105

=> M28208

=> Ml1712

----> poxB

19,50 I· .. · .... ·1· o

.... 1 . .. .. ··· · 1· ....... ·1· ........ 1 . . ..... ··1· . . . .... . I ..... '" ·1·· ..... ··1· ... ~~: ~~ B@dt;,;; <======= X67753

<= X6910B

<- <- <-artJ artQ artP

<- <artM art!

TO

I!!!IB''''' ><=

M31045 X527B6 > <= M23220 L10383

<= M63145

<= M27312 <= Y00373

<- <-cspH infA

cIpA

10

Microbiol. Res. 150 (1995) 1 19

20,00

1· ·· · ·····1····· · ·· · 1···· . ·1····· · ·· · 1· ····· ·· · 1··· ··· ·· .I .Q ..... ··1 ·· ·· ·· ·· ·1· ······ · ·1···

I@bi;.;i _20.16 1l!!20 .2 1 => = > X1741S XS3796 M6314~=2=1=74=9========== = >

011105

L10383 = < YOL25859

<= => M95935 U02275

<= => J03762 M81936

==> M35869

< aat

<-- -- -- > --> cydD

<--cydC

< - -htrO

<--trxB

lrp livR

*pus

- > cOllA

[email protected] = > XOS017

======================== J03412

--> serS dmsA

- > dmsC

-> dmsB

< - -8002103

20,50

6"~Q· .. ·I. ···1 ...... · ·· 1·· .. I 21,00

.. ~I~~·""··h·· .. · .. I ......... I ......... I .... .

@@626.g6 ~=0=B=0""3""5=======

<a ct

<= M2641 3

<foeA

~-fl---

1&6Kd => M25608

=> X00557

--> sere

--> aroA

1&826. ;g 1&626. 8J I&!!!!! . 92 = > => = > X00785 X04864 J02614

~V""0""03~5~2==== =Z=11=7=9=6=======

= > V00342

- > --> ---> - > B0 04845 himD GlsbA kdsS ---> --> rpsA yeaH

""mesA

21,00 ~ ........ . I . ...... I ...... I .. . .. I ... ··I~ ... J .. . ..1 .... · .... 1· .. .. · ·1·

»

&@8;1 .68 026440 <= X639 80 X57550

--> kicB mukB

-> kicA

> 8004846#

B.Ut <= X03629 <= X05904

<= <= M744B9 J0556S <= X68192

<=

=M1==S2==73=== = >

J01655

<--aspC

<--ompF

X04020 <==== ==> M3 3 145

<---asnS

X03709

<-pncB

M15676

pepN

···· 1····· .. ·· , ·· .. ·· .. · , · ·· .. ····,·· · . . ·· .. , ......... , ......... , .. . ...... , ... . ~.60 '&E86~~;'i~ .• ~g~ .......................... 'a'E~8~8~;'f-. I",. .. = > X02B26

--> pyrO

20 Microbio!. Res. 150 (1995) 1

X73888

--> C00 4874

-91tH

<-t:.orR

--> tore

tarA

=> !l33807

<~~========== = > 016500 M99166

<-- ---> cbpA agp

- > BOOU20

*nfsA

-- > wrbA

*uup

1!!I!II!1 . 92 = > X70111 <= JOn86

> rmf

< X70444

<--t.bA

21/50

22,00

11

22.00 / ... ...... / ...• ... / ....... J.

l@dS;. dd I@b;; . is = > V00307 > V00358

=> X66232

> sulA# --> ompA

*expA

22,50

I·····J

J04726

----> helD

. . . . . 1 .

22,50 .J. 'OA" . J ......... J . . .• ..... J .. . . . ... J . .... .. ~.J. ·0······

II!I!II!! . 20 awas u g = > X00547 > K01555

- > yccA

================ = > 1134825 X05471

"'S=63=S=1=1====

hyaA :> hyaC h;aB ----> --> hyaB hyaD appC

--> hyaP

= > MSS 70 S

--> appB

> yeeB --> appA

23,00 .. ·1 . , .... .. ·1· · · · · ... -±- ....... ·1·· ....... J .• , •.•• • . J. " •.....

\!!!!022.80 &@622.85 => 012597 =X6=U=97====

---> mdoG

mdoH

23,00 I···· ·····1· ····· · ·· , ··· · .. ···1· .... ····1··· ··1·· . ...... , . ....... . , . .. ...... , 23,50

. 1 . . .

1&023.00 => L04979

-> csgA

23,50 / . . ./.

&@053 .50 = > 010391

-> ycdB# --> phoH

.. dMbSJ .17 <========== U05212 <============= X7S340

< C005252#

< putA

<= M35174

X05653

< -yedA ---> putp

?hslD

IC0023.40 > X64000

*tolD ... tolB

. . ... / . . .. .. .. J . .. ' " . . , . • . .. .. ·i' ········ . J .. .. .. ... J ... .•• .. . J. 24,00

. .. . . , .. . .. . .. .

1Q&!I!3 .75 = > X52787 > X02802

12

Microbio!. Res. 150 (1995) 1 21

1 ~~: ~~. ···1· ....... ·1· ... . ... ·1··· .... ··1· . o 1······· ·· 1·········1·· ··, .... 1 .. ··· .. ··1 ··

d&M2t.8k < X59939

<====== X61000

< - <---mayB htrB

<---- ----> yceS yceA

liQSi .n = > X04469

= > M16752

--> yceB

----> pyrC

. B!r.'28 = > M99278 => X06118

--> ri~

- > yeaH

-glnR -ElgA -ElgB -flgC -flgD *ElgB

24,50 1 ......... 1 ........ 1 ..

0. · .. 1· . • . 1 .

c · ..... I ......... I·· ·· .... J ...... · · I ......... I .. .

aRib U0251~ ~=~~2~7=4~7============

<======== X67470

=> > => M29~98 M77744 M84991

===>=> M96791 M9~79 3

= > Z34979

<= L23942

=> MB7040

<===== X54309

*flgG *fl gH *flg1 *flgJ

<--- ---> --> ---> -> me yceC# yceD fabH fabG

- > ---> ---> -> 8002470 plsX fabD acpP

<- -> yceP rpm!'

----> fabJ

*fabF

~O => LOB83 => L04577

---> helB

*tmk

24,50

-ngF

25,00

II!!!!!! 4 . 98

J02618

ptsG

25,00 I .... .. I ~· .... · 1 .. ·

25,50 ·1 ·· ·· ·····1···· .. · .. 1 .. · .. ·· .. 1· ··· .. · .. 1· .. .. .. .. 1 .. · .. .. .. 1 .. · .. ·· ..

I@854 .;' => J02618 <===== X17615

-> ptsG

< fhuB

25,50

~O = > M93135

---> pabe

IEMg ,qg => V00306

-----> ndh

?mfd *th iK

'@big .16 ~~6=4~51=9~===========

<---- <--- > potD potB pepT#

<- <----pete potA

1 ...... ... 1.0

...... 1 .... . . • 1 . ··1· ...... ··1 ...... .. · 1· .. · .. .. ·1·· .. ··· .. 1 .... ·····1·· 26,00

U@S;§.§6 ~~9~03~9~3~=========

<= MaH33

<===== M74924 <======= X59307

<----- <----- < - -phoQ purB ycfB

<-pheP

<ycfe

22 Microbiol. Res. 150 (1995) 1

A

1!!fIlIIII!P.'66 = > J02799

----> icdB

~4 = > M19634

---> lit

"'sfic ?icdC

»@651 .;g X01805

K00676

---> ycfA

pin

13

26, 00

I····· ····1· o

B!5 .95 > X01805 > K00676 = > Zl9l04 => M76667

- > mcrA

··1· A

~026 .07 = > M19 6 83

26,50 ·· · .. ·1 · .. .. ·· .. 1 .. · · .. .. · 1 .. · · .. · 1 · .. · .. ·1 .... ·· .. 1 .. · .. ·· .. 1· .. ·

[email protected] < J03153

*frdR

< minB

<-minD

<mine

1&6;g .t1 M101 07

= > M13387

--> umuD

<= L03721

<= M8 3 655

<- .. dsbB

- --> umuC

27 , 00 26 ,50 b· .... ~· .. b· .. ·· ····I .... ... 1 . . .. ..... 1 . ........ 1.; ...... . 1 .. . . . . . .. 1 .... . · .. · 1· · ·······1·

dEBdSi.it = >

L03721X08087

1'183655

<---> nhaB fadR

27 ,00 I .... ·· .. · 1·

I CD026.96 > U02423

> gtp1#

27,5 0

I·

d@d;' .19 ;;; = > X6L3 664 9 = > X15996

==> M1 7B07

=>

*rime

· 1·

M1l 586

--> narK

==> XOl164

X16181

narG

*terD

IE6821 .18 Ln948

<-- - -> 80041 09# dadX

--> dadR

*ups

,@oSk . 9t X15868

---> treA

ID@lIIIIIbl!lS!'lg!".l!Ig"g = > X61941

U02423

- - > pth

gtpl#

?terC4

·1 ·· . 1····· · .. 1 .. · .. · .. · 1.... 1· ·· ...... 1 ...... .. ·1 · ... · 1· 27 , 50

• Q

d@629 .B => ===~=== X53983 M3 078 5

= >

<= 1'113174

= > M2532 3

D28567

<= M77237

= > M1l5 19

= > X17434

<-- <- --> pre ychC prfA

<-- --> - > ychB hemA hem!(

= > X05552

--> 8004919#

-> ychA#

- > kdsA

I&b2J .31 => D10264

- > hemM

[email protected] <====== X13360 ~=6=9=1=8=9==========

~':'2=4 "'91:=0:===== <= X14884

<-narL

X15996 < X65715

<---narX

narK

.. .. I·· · .. · · · 1· TT ·1 .. .. • .. 1 .. · Q .. I ~. Q .. .. 1 .. .. • .... 1 • . • 1 • . n.

29.00

= > < H2014 7 K00266

<= KOll97 <=

= > X66 00 3

<= X07688

=> = > M98830 X5373 3 = >= >

X0416B <=

X59940 X51523 <=

---> -, narH narI

- >

M1074 2 <======= M6467 5 <= L20251

X57231

X6 732 6 <= Y00976

<-- - - > --> -- , tge# 8002467 galU tdk

<- <- - --, < - - <--

~':'5':'9':'2':'63:=============== ES3072 < <========= X59502 X595 01 ~=3=3=50=4==== =M~6"'09:=1=B====

= > J05433

<..,----adhB

> X7014 7

<-B004585

narJ c pr y chJ# hnr hnsA ychG < -8002135

oppA

14

Microbiol. Res. 150 (1995) 1 23

2B,00 I··· · .. · .. 1··

teBb; 9 .45 ES3072

*oppB*oppC *oppD

. . I . . ·1·····1··· ·1·· n n « n 2B,50

··1··· ,· ··· ·1·· ·······1···· .... ·1····· .... .n All

dtOO9g.iS <======= L12044 ~~1~3~5B~3~~~~~= ~~6697 < U01911 <= U159B6

<---

~>

M10B92 => K00431

< JOl714

<~

X1669B

<-- <- <- --> <- <--cls kch yciJ yciB yciD yciG trpB

-> <-tonB ycic

<--yciA

*aroT

<-- <yciB trpA

<--yciF

<~

K01155

< K01792 <= M3B366

<--- < trpD trpL

<-trpC

<---trpB

2B,50 I ......... I ......... I ........ ~ I . . . . . . • . I ... .

29,00 o . ·1········ .1. 0 ...... ·1········ ·1········ ·1·········

d@oSi. 50 => X67913

---> =b

M!l!8 <= M2152B

<-btuR

.56 i@62Ui => M73320

X04475

--> aohB tapA

=> M15041

X60293 => M34332

--> eyeB

acn

<= X67876

>=> M23628

--> pgpB

<ribA

*mvrB

IidSB!l8 .88 ~>

M23250 => J0276B

-> pyrF

<= M22B59

-> yciH

< osmE

*crg *dnaL

I:~: ~~ .... I ......... I .. . ...... I . ..... . .. I . . ..... . . , . . . ...... I ......... I ... . ..... I ......... I .... ~~: ~~

DEB63;.68 => UOB190

=> M97219

> -> sapD# envM --> sapF

I 29,50

·1

*qmeA ?hsl?halF ?halG

. I . . . . ·1·

&@6;;.16 X52284

--> prrB

--> prrD

--> prrc

24 Microbiol. Res. 150 (1995) 1

?hslH *trkB

B@bS;.31 M38433

---> aldH

[email protected] X57560

-> -> pspA pspB

-> pspB

-> pspC

-> pspD

. I ......... I ......... I . ........ I ......... I ......... I .... 30: 00

dEBu;;.I4 => M12114

---> tyrR

*linB

15

30,00 ~ ........ ·1···· ..... I···· ~ ... ·1· · ··

ICD030.00 d6Bd1b.6; > X63985 > D21139

<= J01608

~daA#

<= Y00495

<fnr

<-ogt

?nirA

30,50 I·· .... ·1··· ··1··· . . . I . . .

Q Q

1!!II!0.55 => Z23096

< aieB#

~3 => X56783

---> trkG

30,50 .;1. ···1· .. ··· .. ·\··· .. · .. \ .. .. ·····1··· .... ··1··· .. ····

~O => X52647

--> dbpA

. .. ·1 .. · .. ··· ·1 ......

&88616.16-======== <=< ... L23927 M24905

< <----ydaC# recB <- <-ydaB racC <--recT

*oriJ

31,00 ·1 .. ·······1 .... · .. .. 1· ........ 1··· .. ····

[email protected] M17609

X04114

pac

=> M12373

'*'hslI *hslJ ?htrH *ksgD

31,00 I ........ 1 · · .. 1 ... I . . ....... I ......... I ........ . I ......... I ......... I ......... I .... 31: ~O

31,50 1.·· ...... 1 ......... 1 .. ; ..... 1.

IMi!.§; X62680

=> V00279

=> X00792

---> IS2

---> IS30

.. ·1·· '0" .0. 1 ........ ·1········ .\ .. . ...... \ ...... . . ·1··· 32,00

-> ydbC

YdbD#

16

Microbiol. Res. 150 (1995) 1 25

32,50 32,00 I·· ·1·········1·· ·······1········ ·1········ ·1······ ···1·········1·········1·········1········· • »

~2. 00~5 I&dB .26 => => <= L09068 M64541 L09067

=> X07569

-> ydcF

---> ald

<gape

-> cybB

> ydcA

> ydcB#

I!!!I!!I!IfP.! 0 => K02073

---> trg

II!!!II!! . 30 => X15860

--> rimL

132,50 ... 1 .... . .... 1 ... . .... . 1 ...... . .. 1 ......... 1 ......... 1 . . ....... 1 ......... 1 ......... 1 .... 33: 00

db&52.86 L19083

X60998

----> rhsB#

--> ydcO

=> L02372

--> ydcC

-> ydcB

33,00 33,50 1·········1····· ·· · ·1··· . . .. ··1· · ·· · ····1·········1·········1·········1·········1·········1·········

[email protected]; <======================== Xl7110

<- <--narV narY

<narW

<-------narZ

*asu

1&8§jj, <================= M75029

<-- <--fdnH fdnI

<-----fdnG

'@BS].46 => 000927

<--nohA#

1I!!IP.I'3 . 4 8 <= X55956

<= 013179

<-"sfcA#

33,50 1- ... ~ ... ·1 . ... 1 . . . . . .. 1 ......... 1 ......... 1 ...... . . . 1 .. . ...... 1 ... . ..... 1 ......... 1 .... ~:: ~O

eE8053.48 X55956

013179 => Z11493

=> X57433

-> sfcA#osmC

<-rpsV

26 Microbiol. Res. 150 (1995) 1

I&S§]. 98 1@6]].;6 =X7~1~9=17~==================>

X73295 => M84025 => X63123

-> ---> ----> yddA B004835 gadS ydeK

----- -> yddS B004836

17

34,50 34,00

I·· ···1·,· ·· · 1·········1· .. ···· · ·1·········1·········1·········1·········1···· · ····1···

d&8Jt.h 1161242

hipA ---> hipB

34,50 35,00

1·········1·········1·········1·········1· · ·······1·· · ······1·····,···1·········1······· .. 1·········

1!!o034.60 => 1123252

*qin *sad

135

: ~~ .... 1 ......... 1 ... . ..... 1 . . . . . . . I

1I'!!!'II'34.80

X57947

dcp

i£@6it.Ig 10196235

> L06966

<= X57947

--> --> --> <-- --> ydeA marR B004688 ydeF ydeJ

-> yde8

<- -> ydeB ydeD

-> marA

?marB

<---dcp

35,50 . ..... I ... . .... . I ......... I ........ ·1 . ~. ; ... ; . I ........ .

k@035.;' < X02405

<relB

<~

relF

[email protected]£ X07465

<dice ~~>

dicA

~ > dicB ~>

B002164 <- - -> relB B002165

> 8002166 -> 8002167

--> B002163

. . . I .... . I .... . .... I . . .... . .. I .. . ...... I ......... 1 ......... I ......... I .... 36: ~~

!!CD035.41 M'!J5.56 > X07465

=> L28429

~>

cepB

*dgsA *metQ

18

Microbiol. Res. 150 (1995) 1 27

36,50 36,00 I ·· ... ·1 ···· · ·· · 1· · ····· · ·1 · ···· .1 "0 . . . . . 1

« ······ 1·· · ····· 1·· ·· 1·

&@6]i.61 ~=0=4=1=9=5====

<====== X660B6

<---pntB

<---pntA

1@8]k .£; => X75466

D90037 <= M23251

< X04065

~:"0=0':'5':'2':'2=== = > MiS380

- -> <--- - --> uapT# fume manA --> <---tUB fumA

&&6jUS 1I!!IrJ'6. 48 <=====<= M14641 Dl0497

<= X02395

<. <-uidP# hdhA <----uidA

*uidR

1 ~6: 50 .... 1 . ... . .... 10

" .. ... . 1 .. . . . ... ,I .... . .... 1 . .. .... . . 1 ......... 1 ......... 1 ....... . . 1 .... 37: 00

HcB85t.id Dl0497 <= H2B539

H60722

<-malI

- --> malX

37,00

=> M5 903 3

--> malY

--> add

I!!I!!:J 6 . 8 0 => J02857

-> nth

II!!IrJ'! . 88 = > L01622

- > rnt

-> rhlF#

1 ·1· 37,50

·1 · ··· · · · · · 1·· . . . ····1··· · ··· ·· 1· · ··· · ···1·········1·········1······ · · ·1······ ...

WEBb3'. 66 <= JOl719

<= N92351

<-tyrS

<--pdxH

37 ,50

t·· ·· · 1 .. ··· 1·· ·· ···· ·1 · ·· · · ·· ·· 1· ··

I!CD03 7. solUM' . ;g > X52788 > K01560

<==================== X69109

<= M98330

<--cfa

28 Microbiol. Res. 150 (1995) 1

<- -ribC

"'kegS

»

1I!!IrJ'7 . 32 &&61' .1 , => J03511

-> sodS

= > X51368 => J04212

--> purR

. . 1 • • • ·· 1· . .. . ··1· · · ·· · · · · 1 IEM]} , 14 =>=> M24636 X68953

= > J01645

= > 542225

*qmeB

• . . 1 • . . 38,00

19

38,00

1·········1··· I · ·· . ···1··· . . . . I . ···1·········1·· ·· · ····1···· · ····1·········1·· »

=> X04306

--> ydiB#

-> aroD

d@6§iJg M69116

X59381

ppeA

[email protected]± => J04221 => M38266 => V00261

--> ydiA#

=> X04373

--> aroH

*ydiC

38, SO

I· ···1· 39,00

. ··1·· ····· · ·1··· · ···· ·1·········1·········1·· ·······1······· · ·1·········1····· ....

[email protected]± <===== <= < M14031 Ml0423 M13549

<=============== K02844 <========================== V00291

<-- <-- <-nlpC btuB himA

<-- <--btuD btuC

<:-----pheT

39,00

<= M13252

<= M13251

<-- <pheS rplT

< <----pheM thrS

<-infC

~.82 B&638 .95 => K00128 M55161 => K02500

--> pfkB katE

*rimB

1·········1·· ···· ·· ·1· . . I . . . . 39,50

·1··· ... . . ·1· ·· ··· "8 I···· .... ·1········ .1 0" ..... ·1· ....... .

Ic8638.;§ M55~6~:=5=2=8=9=0=================M=1=5=3=2=8>

<= <= <= X66725 M93570 X75957

--> katE

<-- <--celF celC

<- <E002988 celD

<= X60186

<-

eelA <anr

<-- -> eelB ntrL

~039.30 => X63983

~.39 => X13002 => M22592

--> xthA

< M33735

39,50 I·· ···1· · · I .. I ......... I ......... 1 ..... . ... I ......... I ......... I ......... I .... 40: O~

&888S,,«; .a~68~8'j; ... g .. , .............. .. =><===== <= K02499 J05076 => M23171 => X00988

M68961

<= M30184

M13359

--> <----gdhA topB

<-- ----> selD eppA

?halK *feo *pneA

nEBbjY.;6 M26934

--> ansA

*ackB

20

Microbiol. Res. 150 (1995) 1 29

40,50 40,00 I······ .. ·1· ........ 1 . ···1·· · ·· ·· ·· 1·· · · ·····1··· ·· · 1··· · ·····1········ ·1··· ······1··,··· .. ·

*mng

~05 => X02662 => U07751

--> gapA

*dnaI

I~O,sO .. 1 ....... 1 ......... 1 .. .. ..... 1 ......... 1 ......... 1 ......... 1 ......... I ......... 1 ... u,oo

1&040.50 => L04572 => L05381

> holB

>

d&846.H L02649

X70994 => X070S5

---> fadD

--> rnd

~70 => K02673 => U07748

---> pabB

*semA

d&6t6.;; M28695

---> sdaA

d&818." J02699 => M36404 => Xl7443

--> --> manX manZ

-> manY -> ptsP

--> ptaM

141: ~O ..•. 1 ......... 1 ......... 1 .. 41,50 ·· .. ·1 .. · .. ····1·· ...... ·1·· .. · .. ··1·· .. · .... 1 .. ··· .. ··1· .. ······

;rkdgR

41.50 1 ...... ·1

[email protected] 1187458

X63694 < M55005

edd <---zwf

1&6".61 => 028496 => L28430

> cape --> B004753

*cpxB

. . . . 1 .

30 Microbiol. Res. 150 (1995) 1

U8&Ci.28 <= 1158470

~"'0"'0"'6='74==== <==== 1175634

<htpX

<----pre <----tap

[email protected] L20897

<==== 1187458 <==== X63694 <= X68871

--> purr

<yebF

<yebG

<-eda

<--edd

.. I· I .... Q

·1· ........ I· ........ I ........ ·1 ......... 1 .... ~~: ~~ [) «

gLWCI.I; => M63703

<===== M77039 <= M87660

---> <-pykA yebA#

<--mobS

Bon.77 => X75948

-> B002994

> B002995#

I@64£.85 ~"'21:=2:=9:=8:===== <=====

~='5='3"'86"'3~===== <=

X07091 < D10165

< X59551

X53984

<-- <- <--ruvB ruvC yebD

<- <- <---ruvA yebC asps

?dinY

21

42,50 42,00 1········1· · · · ·····1· · ·· · I · ... I; ........ 1 ......... 1 ........ ·1· ..... ···1··· ... , .. I· ....... .

'!!CDon.83 ib&d.2i X53863 X15320

---> argS

ib&i3.JB ~~13~4~6~3~======= ~13462 ~~6S2

<== <==~~=========.<========= K02175 J01705 M34669

<======== <== V01504 M12914

<-cheZ

<-

<-- <--- <----cheR

cheY <--cheB

tar cheAl <---tap

<-cheW <---cheAl!

<-motB

*ssaG *flhA *flhB

42,50 I· .. ·1··· · · · ···1··· ... ·1· ·0·· .. ·1· ..

iLWtS.iS Q

·1 · ···· . 1 ......... I ......... 1 ......... I .... 4:: O~ c:Tc: c:c: «

§ESSiS.56 J01652

<= M1291M19439

<-mot8 flhc <-- <--motA flhD

< X69160

<= K00420

<:================= X06091

<---otsA#

<otsB

<--araH

<---araG

<= X58969

<-araF

i&6i@6t2 . 83 => X53513

=> M13768 => M23240

--> ragA

--> tyrP

1@612.;6 <= <= X03239 X05398

<== X52789 <= M12299

< X03691

<- <----yecA uvrC

<-pgaA

<yecB

43,00 43,50 1 .. ~ ...... 1 ..... . ... 1 ......... 1 . . ...... ·1· . ...... ·1· ....... . 1 ... .. .... ! ......... ! ......... ! ........ .

'l!&l!t2.90 AMt§. it 1&!r3. 3B 1&!!f3. 4B < => <==>

X03691 X17440 L01642 MB4992 L13243 M127B4 ~:=1=4=3=5=B===H=B=5=2=4=0=== ~L~1~3~2=779===============

<= J01607

<-- <- ---> --> <- -> <- - --> --> sdiA yedB fHD fliT yedD yedF fHB fliG fliL

<--- -> --> -> > £liC flis yedB yedG fliF# fliH

---> amyA

>gltH ?fliH *fliI *£liJ *£liK

43,50 44,00 1·········1· .... ····1· ........ ! ....... ··I·········I·········I·········!······ . ··!· .. . . .... ! ........ .

T

[email protected] I&fto. 5B 1&043. 6B [email protected] 1&043. 9B =>=> => M127L21994 M5B003

==> => M26294 MBll91

L22182

--> -> --> fliM flip rcaA

--> -> fliN fliQ

-> -> flio fUR

<= Z1l491

< X13330

M:=3=2=30=7=====

--> <---yedA dcm

<-vsr

*nuvC

=> M10746 > K01667 > X015BO

22

Microbiol. Res. 150 (1995) 1 31

44_00 44,50 I ......... I ......... I ......... 1 ......... 1 ......... 1 ......... 1 ... . ..... 1 ......... 1 ......... 1 ........ .

T T T

ICD044.02 ~O ICD044.20 ICD044.25 lcab't.'; > X52790

=> M30469

amn

*aepS

> X52791

*shiA

> X52792 > K00164

*flu *azaA

U09B76

--> rfbB

-> rfbD

rfbA

44,50 45,00 1 ......... 1 . ... ..... 1 ......... 1 .. .. ..... 1 ......... 1 ......... 1 ......... 1 ......... 1 ......... 1 ........ .

A »

R&btU; U09B76

U03041

-> rfbA

-->

--> --> -> 8004135# rfe

rfbC --> rfbX

?galF

8004127 --> -> 8004132 8004118

--> 8004103

i@6"_ ;1 U00009

=> J02641

<yeeA

<-yeeS

<--yeeC

---> <-veeP sbeS

<-yeeD <-yee8

M56tl-.gg => V002B4

X13462 > V002B5 => U02070

X03972

X52656 > hisL -> hisG

hisD

I ~~: ~~ . -.. I .. . . . 1 . . . ()

. . 1 ......... 1 . .. " .. .. I ......... 1 . .... .. .. I .. .... .. . 1 ......... 1 .... ~5: 5~ »

R&OU"; Xl3462 => X03972 => X52656 => U02071

X03416

=> U02072 => X03974

-> --;;> -;;> -;;>

hisD hisS hisA hie! --> -> -> hisc hisH hisp

45,50

B&8t§.15 L04596

=> M23181

=> K02072

L27646 => M63B21

-> B003003

---> rfbK

---> ---> rfbM gnd

*cpsA *cpsC *cpsD *cpsB

1 ........ 1·· . . • 1 . • • . . . 1 . . .... 1 .. • ..... • 1 ....... .. 1 ... .. .... 1 ......... 1 ......... 1 .. .

~5.50 => MB9934

-> rol

*non

dEM1§IEBb¥g.ii <= M90069

<= X71492

~=1=1~7~2=1======================

<= M77127

<ded

<udk

<--- <--epeE yefC yefA#

<--- <--yefD yefB

<---epsG

?ptsF

32 Microbiol. Res. 150 (1995) 1

46,00

23

46,00 1· ··1· 1· ·· ·1 . . ·1· .

dCb6ti.lg <= K02H8

<aIkA

46,50

··1· · ···· ···1·· · ·· .. ··1··· · ··· ··\····· .. ··1·

~47 X798)7

--> gaty

gatZ

wior

47,00 · .. 1 · · .. 1 .. · .. .. · ·\·· · .. · · · · \ .. · ·· .. · \· .. ··· .. · \· ··· · · .. · 1··

dEBBie.i) X79837

-:> -::>

gatZ gatB -> gatA --> gate

--> gatD

<= D12598

- > gatR#

1 47, 00

·1········· 1· .. ·

M8t ,. 85 U00007

. . • • • 1 • . » O.

X55791

o 1·· .. · .. .. 1 .. · .... .. \ · .. .. .. .. \ ...... .. ·\ ·· ·

47,50

=K=0=2 =6 7"'1==

<-- <-yehA yehC yehE metG

<,---_..:. <- - <--yehB yehD mrp

*molR *atoA 1ratoB *atoC

47,50 1 .\ . .. . · · ·1 .. ·· . . 1 . . . • 1 . • •

k@Bi9 . 85 U00007

- > yehP

- -> <- --yehR yehU

<yehW

<- -yehY

--> -> - - > yehG yehI yehK yehM

--> --> -----> --> - > yehF yehH yehJ yehL yehN

--> yehO

'/ratoD

Q 1···· .. · .. 1· .. · .. · ··\·· · .. · · · · \ .... ·· .. ·1···

«

<===== U15049

= > 1'110038

X01067

<----- <-- - > <-- <- -bglX yohB yohD yohG yohI

yehP

48,00

----> <-- -> <-- <-- - --> < - <- <yohH

-- > yohJ yehQ yehS yehV yehX yehZ dId yohC yohB

<- <-yehT yohF yohK

24

Microbiol. Res. 150 (1995) 1 33

48,00 1···1· . . . I . I · .. 1 ..... .. . . 1 ....

0 .... 1 ......... 1 ... .. .. .. 1 ......... 1

48,50

~5§686t'.65 ==================================================

U00007 => X16419 => M60916 => X63144

:> --> yohK yeiF --> cdd

<================== M59444

<--mglC

<= X05646

<= K00419 <= X62 52 9

<-mglB

<- <--- --> yeiA mglA galS

*mglD *terF

<= <========= X63910 J04229

<====== X6s029 <= M19295

<-- <====-- <---folE cirA lysP

=> M22591

--> --> <-- <--yeiH yeiI yeiK yeiM

-> <-- --> <-- -> yeiG yeill nfo yeiJ yeiL

48,50 I· .... 1· .... .. .. 1 . I .. ....... I . ..... . .. I .

49,00 · .. 1····· .. ··1···· · ····1 .... ··· .. 1······· ..

[email protected] U00007

<====== M23196

<yeiN

<--yeiC

49,00

<= X53948

<--fruA

<--fruK

--> yeiO

> 021148

---> yeiQ

--> yeip

*fruB ?fruF

-> yeiR#


--> yejA#

-> yejB

=> X63703 013326

--> yejB

<- --> - - > yejG bieB yejI

---> <-- --> yejF ber yejH

yejJ

I . . . . . . . . . T

·1 .. .... ·1 · 49,50

. ... 1 . ........ I .. .. ... . . I ........ . I ......... I ......... I ....... ..

sEBbi8.do U00008 => 013326

=> X52793

=> J01734

=>

<= 854754 =>

L11273 X61951 => M60876

- > > <----- -> <-- <-- <- <-- <-- <-rplY yejL yejO narP yejQ yej8 yejV yejX yojA yojF <-- ----> yejK yejM

49,50

--> ISS

1· .... · .. ·1 .... · .. 1....1.

IEBBld.db U00008

< X12774

<= <= J02607 KOOs41 <= M10211 <= M10315

<= M13155

-- <--- <-- <-yojH yojI ada yojL

<- <--alkB yojK

34 Microbiol. Res. 150 (1995) 1

< M23214 > M23215

> X04888

<-- <---- <-- <-- <--yejP yejR yeju yejY yojB yojG

<- <-- <----- < yejW yejz yojc aeg-46.5#

-- > eeo

<--yojH

.. I ... .. .... I . ........ 1 ......... 1 ......... I ..... . .. . I .... 50: ~O

YEBdlY.ib ~:~2~8~2~4~2================

S37~60 ~L=13~0~7~8~================

--> ----> resB E00410S

<------rcsC

--> antizyme

25

50,00 I . . . . ·1····· ... · 1·· .. ··· · · 1· ....... ·1· ·· . .... . 1 ..... 0 .. ·1 .. ·· .... ·1· .. .. ·· .. I··· ..... ·1

18SiI'6.5) <:================== Y00544

< X06373 <=========== X57174 <========== X06744

M87509

<- - > pufY# ubiG

<------gyrA

1&6.6.46 K02672

?inaA

50,50 I· ··1· · · .. .. ·· 1· .. .. · ·· 1 .. · . · 1······· .. 1·· .. 1··· .. · · ··1 · · · · .. .. · 1·· .. ·· .. · 1···

1886g6.48 K02672 ==> X0 6999

--> nrdA --> nrdB

I@Sg6 .48 <==== ================ X56907 M20938

<= Y00536

=> M33739

51,00 1··· .... · ·1· . . . I . . I .... ·1 . . . . I . ···1 .. ····· .. 1· ···· · .. ·1 · ·· .. ··· · 1··

51,50

'i~E~BRg't'£·.~6E,. .................. .. < L35031

<= M9 3421

<= <= L0725 6 L35030

<==== L04464 <========= M21797

<--- <- - <--menE menS menD

<-menC

<-8004750

< 8004837

1 · 1 .... ···· ·1 .. · 1· .. ··· .. ·1··· .... ··1····· · · ·1 · .. ···· .. 1 .. · .... · 1·· .. · .. ··1· ·

&880§£ ,kg => M99414 L25055 L19568 = > X7 454 6

=X=6=83=O=1=============================================

;. - - > --> ---> ISIH# nuoA nuoD nuoF --> genR

- > nuoB

- - > nuoC

--> nuoE

*nfrD

nuoG

--> - > ---> nuoH nuoJ nuoM

--> --- - > nuo! nuoL

- > nuoK

--> nuoN

50,50

X06999

nrdA

51,00

51,50

52,00

26

Microbiol. Res. ISO (1995) 1 35

52,00 I·· ····1·

d@6§S.65 = > M22956

I··· · .. · 1·

D21123

- -> ackA

?pta *"hisJ

52,50

. ·1· ....... ·1· · ·

""".20 <= Y00455

<-hisP

< - -hi.!I#

···1 · .... ··1··· · ·· ···1· · ·· ····1· 52,50

I@S;;. if <=========== <========== <======= J01666 J02BOB X02743 <========= V00322

J=O=2=8~OO~===================

<= M68935 <= M26893

<= 1168934

<== M29962 <== M15541

< - <-- <--- <- <-dedA usg1

<-- <-argT# cvpA folC

<-ubix

<purF

<-declO aceD hiaT

<--pdxB

1· 1 ... ··1··· ··· 1· ·

~9

··1····· ·· ·· 1· ·· · ·· · ·· 1· · · · ·· · · ·1 ·· · · ···· 1·· ·· ··· · ·1· 53 , 00

1!PII'52. 31

M29962

M15541

pdxB

I

> diV#

53 , 00 · 1

fiEBg§] .63 M60607 = > Y0055 2 => X7044 5

--- > fadL

53,50 1······· ·· 1·

*amiA

= > M24427

--> fabB

*ssaE

&@655.21 <= X16909

<= M27714 <= 1133021

<- <--yfcA aroC

<--mepA

*trmC *prrnB

. . . . . I A · t· · · ··· 1· · · · ·· ·· · 1· · · , · ·· ··1·········1 , ···· ····1· .... ·· ·· 1···

awg;s. ij U11296

=> X52794 > X05852

--> BG04732

····1····· · · · · 1· ·

~.60 => X63740

--> eacB

" I . . . . . . I . .

aMg] ,k; <= M19035

=JO=16=03=

<- - - > dade dadA

-- > y fdA

···1· ·····1·· ·· ·· · ··1···· · ····1··

~80 => X74825

--> nupC

36 Microbiol. Res. 150 (1995) 1

53,50

54,00

27

54 . 00 I · ·· ·1· ··· 1·· .... ·· · 1 .. .. .. · · · 1· T I · · .. ·hIT .... I .. 54.50

· · · 1· ...... ·· 1 ..

'g l k

54.50 ·1· .. ··· ···1 .. · I·

DESa§t.53 M21451 = > X12615

= > Ml0425

M21994

J02 7 96

= > M93578

-- > - > -- > cysZ# pt sH err --> ---> cysK ptsl

·1

[email protected] <================= X63976

<= <= X5279S X55737

< > M13687 X17321

= > X52796

= > M10745

<-- < - <-yfeA B003013 yfeB

-- > < -y fe C gltX

dUM .46 <=========== M24278

<========= M3 025S

<----lig

'i'xapA '*'xapR

... 1 .... ... . . 1 . .. .. .. .. 1 ..... .... 1 . .. ..... 8

1 .. . . . . . I . .

UCB8ki.t; <==================== M32101

<-- <-- <--cyBM c y aW cye P

<-- <-cy sA cysT

d@Ss,. ;6 X75413 <= X63986

--> B0 04100

--> hemP

<--y feG

55.00

55,00 I ·· .... 1 ·· 1 .. · .. .. \ · .. .... ·\ · ... \ ... .. .... \ ......... \ . . . I . .

55.50

§@055.66 D124 7 3

----> tktB

55.50 II .. .. ·· ··· 1· . . . I .

IEBbgg.tig X57403

= > 541760

> y ffA#

> y ffC#

-- > y ffB --> dap6

· ·1 · ·

WEaSg; .56 ~:'3=4 =01=1======

= > X65714 = > M94724

<- -B004708

---> narQ

<----aegS3.0

. . . 01 .. . ...... 1 ... . . .... 1 ..... .. .. 1 .. ....... 1. 56.00

kE6g;, IS < M33928

=> M63 654

<= X57402

<= M12844

<- <--purC dapA

--> bcp

<-- - - > nlpB yffD

> yffF#

- > y ffB

28

Microbiol. Res. 150 (1995) 1 37

. . 1 . . ·· 1· ········1·········1······ · · · 1·········1 · · ·· ·· ·1····· ····1·· 56,50

1&056.12 = > M15 737

-> uxaB#

sn.n <=============== X73586 111374 7

= > L06129

<= === X57104 L03719

<---- ----> uraA purll ppk

<- - > ---> upp purN ppx

[email protected]. <===== M10101

<----guaA

<gua8

156 ~5 ° .... 1 .. .. .. . .. 1. ·· 1·· ······· 1· ·· ... . ·· 1· ······ ·· 1· ········1··· ·· ····1· ·· · · ··· · 1 5 7, 00

[email protected]; M10101

= > J0259 9

guaB ---> xseA

. . . . 1 .

d@8§; .bl \101827

\105338

-- > yfeH

i@Ski,kS <= M11843

<= J01629

<= X64451

<--- <- -hisS yfgA#

<---gepB

·· 1····· ·· ·1 ·

= > 1188 65 4

--> fdx

[email protected] => M34828

--> suhB

- ---> hscA

*ilvF

[email protected] < \102965

< 021149

<= X57555

<---yfgB

<ndk

*ssyA

57,50 ·· 1· ·· ······ 1·· ·· ····· 1· · · ······ 1··· · · · · · ·1 · ······ ..

KCBbg; .16 <= V00283

<= J01621

X58872

<= S67014

<= 1116778

<---- - --> glyA hmpA

< glnB

<---y fhA

57,50 1······ ··· 1· ···· ··1···· · · · ··1· .. I .. . · ... . I .. · .. · ... I · · .. · .. Q.I ...... ·· . I .. ~ ...... I . .

58,00

~57. 4 0 567014

< --B004110

IUE~8~S'g~;·.'g~b ........................ • ICBSg) ", <================ <== <= <======== M19501 1174526 M14658 K00426

~=7=2=33=6=====

<---------- ----> <-purL yfhO yfhB

< --yfhC

*azl

<= <= X02673 M76470

~j

<=<= 1127251 X02946

<= M26416

<recO

<-m e

<----lepA

<-- <--era lepB

38 Microbiol. Res. 156 (1995) 1

29

58,00 I········ ·I····~· .. I··

[email protected] X03954

---> gshA

58,50

·1·········1····· ··1 .. .. · .. ··1 .. ·· .... · 1 .. .. · .. ··1·········1······ ..

BEOOki.n ~47 D13169 M58699 <= U10148

=> X12714

X14152

<-- ---> ---> mclA# nadB armS <-11004270

?rpoE

=> J03725

-> 11004268

*ranA

-> E004271# -> ung

--> 11004269 pSBA

58,50 1 ........ ·1· ·· · · .. ·1 .. ·· · · ··1 .. ··· .. I ..

.0

59,00 .I .. ....... I ......... I ......... I ......... I.~

[email protected]) => <= M58699 X52363

< X75467 <= X53027

> < pSBA rrfG <--kgtP

59,00 I ........ ) ..

dEBb5i.gg X01818

-> trmD

--> rplS

<--yfiB

59,50 I.. ··1

*rrlG

. . . . 1 . . .

• A

~09 <= X07863

<grpE

. 1 . . .

Mm. 76 <======== M29364

<======= X57620

<-- <-rrs~ __________ yfiH

clpB

[email protected] M10431 => <= M5B024

> V00314

K01989

-> <-yfiA tyrA

<~

K03453

> <--pheL aroF --> pheA

BE86U.96 X01818

--> ffh

rpsP

59,50 .. I· ........ I ..... Q ... 1 ..... . ... I········ ·1· ....... ·1···· I· .

'£~tIlll6I111g"' .... lIIilll!g· Y00357

-----> recN

dEOOh.2§ >

D12501 => X16382

U03737

--> --> smpS intX

--> serA

> alpA

1!!I!059.40 => X52620

> smpA

.. 1 .... . ... 1 .. .... ... 1 . ...... .. 1 ... .. .. .. 1 ..... .. .. 1 .. ..... . . 1 .... 60,00

30

Microbiol. Res. 150 (1995) 1 39

60,50 60,00 I· ···1· . . I . I ···· · ·1· ··1· .. · · .. ·1·········1·········1·· .. .. ···1········

B>

dMgd.dl M88334

=> M38417

X65104

---> ---> gabD gabT

--> gabP

*nalB

60,50 I ···1· ··1··· ·1

TT T< I ··1·

!B~8B~d~g~d~.5~1~ ...... 'B'E~B~d'g~d·.'e~d .. 'i~EBR.6i.g~ .• ~.,. ................ • => <= <=

Z28405 X54151 U07823 X52797

M86657

--> mprA

---> -> emrB ygaC

--> emrA

=> X69210

-> stpA

< <== K01556 L25853 <= <= L07596 V00328

<====== J01581

< csrA

< alaS

<--oraA

<-recA

BCDdtd.37 M24856 => Xl7445

=> X63982 => X52694

--> proV proX

*envQ *lytA

I· .. ·1···

BEBd.S.Bl J02708

=> M93583

--> proW

·b······· · · 1···

=> X51361

=> X13463

---> -> -> arIA arID srlR

--> -> eriB srlM

-> srlQ

61,00 I······· ·1 " · · ·1···· · ·· ··1·· ·1······· · · 1· ·· · ···· · 1··· .... ··1·· ... ····1·········1··· >. Q

61,00

61,50

~d~E"u.d'.± ... S~d ........................ ·.i~L~~~Q'.'i·.i'i .................................................... ... <============= <====~===================== D14422 X17506 M58504

M73326

<-- ---> ascG aseS

<-- ---> hydN ascF

<-hycH <hycG

X54543 M64730 === X52227

<--- <---- <-- --> <--hycE hycC hycA hype hypE ygbA

<-hycF

<-- <- --> hycD hycB hypA

-> hypB

--> -----hypD fhlA

mutS

61,50 1·1 ·1· ·1 ·· · ·1 ·····1···· · ····1· ·····1·········1····· ... ·1··

62,00

~8 => M64730

---> mutS

*exbC

40 Microbiol. Res. 150 (1995) 1

a&&er. 78 <= <= D1354B M63493 <= X16400 <= Z14969

<========= L07942

<= D17549 <= L07869

<-rpos

<nlpD

<pcm

<--ygbe

<--ygbB

D@st!.I' <============= M74SB6

=> M27059

<= => MB6936

<-cysc

<= M62471

M1B270

<= M35098

<eyeD

<--- --> cyaN iap

31

62,50

± 62,00

· 1 . . . . 1 . ·· 1· ·1·· · · · ·· ··1· · ··· ··1··· ······1······ · ··1··· · ·····1·

ICD062.00 > 1'1548 93 > X52 7 98 > K00197

62 ,50 t· ·1·· ·1 · . · 1· ·· ··· · · · 1· ·· · . \ .

~51 fiEB6kB; = > 1'127060

< X82400

=> ES3057

=> IiS3082

>

'WagS .36 1'123008 = > 1'165058 J05057

==== Y07525

---, - > <:ysJ <:yaH

----> eyer

. \ . . . . · ··· · 1· · · · · ·1 ·

<========= <=== ==== 1'11 2 94 3 016450 010888

<--eno

<---pyrG

?mazG

<======== J04039

< mazF barA

<-----r e lA

*mazB *ygaA *ygcA *relX

\ 63: O~ . ... \ . .. . . • , .. 1 • ••. • , • •. \ • • • .... • . \ . . ' . . ••• \ •..... ..• 1 ••.•• • .•. \ . • .

d@B';'yg = > L07 7 63 ~':'7:'34=1"'3=====

<= U01030

--> sdaB

~=3 =1 0=5=9====

X15025 <= 1'12717 7

<-- ---> ---;> - > <-- <--fuc O fucP fucK fucR ygdE gcvA

<-- - --> - > - > <-fucA fuel fucU fucT ygdD

*exo *ahpS *lysX

. . 1 . . .

1!!!ft3 . 39 <= 1'198266

<--metX

63 , 00 . . . 1 . . . .

1866g; .;5 U01233

L 0 7763

--> sdaC

sdaB

I· 63 , 50

I@Sk] .46 = > Y00492

<== X045B2

--> argA

<reeD

63,50 I· .. · · .. · · 1· · ··1· .... ·· · ·1 .. .. · .. ·1· .. · .. · ··1 .. · .. 1 .. · ...... 1 .. ·· · · ·· ·1 .. ·······1· ·

B •

64,00

«

d@6tS. 46 <====== <=

J017l0 X04582 X06227 <========== <= <= X04591 1'11 7 095 U122B9

<================= X03966

<----- <-- <-reeD <==== __________ ~ptr ppdC thyA

<------------ <- <--recB reeC ygdB 19t

<--ygdC

?ppdB

~63.90 => X63984

===> Y00l13 => U16361

- > mutH

32

Microbiol. Res. 150 (1995) 1 41

64,00

I ·· . ·1 · · . . .. ··· 1· . . . .. ·· 1· ···· ·· · · 1· · · · .. .. · 1 .... ·· · · · 1·· .. ···· ·1······· .. I ··· · · .. · · 1··· 64 , 50

164: 5~ •... 1 .... . . . .. 1 ........ ·1 . . ·· .. . . ·1 .····· · · · 1· · · · ·· .. ·1········ ·1······ · ··1·· ·· · ··· ·1· ··· ~:: ~~ I@81'.i8

J=03=7""3=2==== <====== L14681

J01614 = > V00280

<= X00272

- ---- ---> --> <- --aae galR lyeR araB

<-- <--lyeA ygeA

65,50 65,00 1 ... ·1 · . . . .. · 1.. '01 .. .. ·· 1··· .. · .. ·1· .. ··1······ .. ·1·· .... ···1 .. ··· .. .. 1· .. .. ····

aM8it .ib mn.n <======= <===========

X74547 J03795 M548U <= M11520

<--- <--- <--glc B 1 yeS recJ xprB

<-- <-prfB debe

+del *qm.eD *brnT ?bglA "acd

6 5 ,5 0

I · .. · · 1· · · · · · 1 .... .. · .. 1· · ·· .. · .. 1· · ·· · .. · · 1··· .. 1· .. · .... · 1· · · · ··· .. 1· ··· ····· 1 ..

[email protected]£ M97263 >'=L=2=0=8=7=2======> 0=9=0=2=8=1========

X73958 = > M576 90

--> ------gcvT gcvP

- -> gcvH

42 Microbiol. Res. 156 (1995) 1

=> D21144

<--vise

<==> D00398 M12965

<-pepP

<-- <--

-> ear

ubiH ygfB

a@m .'s < = > L29397 M62865 <= N00029

X~66""8~3=6==============

<= 1164630 <= X73026

-> - > <-serA iciA S004119

<- ---rpiA ebln

'poaR ?mukA

66,00

1!!IIf5.98 <= X14436

< yggB#

<--yggA

33

66,00 1 .. •··• .. ·1

d@Sgg.;& X14436

<-- <-yggB fba

···· · 1···· · · · · 1

<-gapB

<--yggD

<--yggC

<yggF

66,50

.1.······· ·1· · ···· · ··1· · ···· · ··1·········1··· .·· · ··1··· ..... .

[email protected] < X72677

<--cmtA

<-

<==== => M32363 K02129

<==== <====== X6B025

<----tktA

M31770

<speB

--> <----

--> metK

cmtB yggG speA

*can TOpS

66,50 I ......... I ..... ~ ... I ..

~.50 => X65169

-> endA

*galP

1!!!!I!!P.'5 B => X01666

--> gshB

. . . I . . . ... ·1········ ·1········ ·1······· .01 ........ · 1·····

~9 'bUtt. 74 IEBSgg. 82 < M34234 M59471 <= => M34277 X52391

<-- --> ansB muty

> X55B13 => X06174

<==== M33766

---> nupG

<----speC

*rdgB

67,00

··A········· II!1066.95 => X02480

> X52799 > K01552 > M25426

67,00 I·· ··1 I · · ··1 ... ····1···· ··1· · ·······1······· ··1·········1···· ····· 1··

67,50

'glc

~o => X53819 => M57382

--> kpeM

1I!l067.30 => M573Bl

--> kpsT

*hydL

16:: ~~ .... I .. • . . .... I ......... I · .... . . .. I ····1··· ······1·········1·········1······ ··1···

6B,00

[email protected] X7456=7====

M64977

--> -> --> kpsB kpeU kpeS

---> ----> kpeD kpsC

?alx *ecfA

g@agl. ;5 X60598

M76370 => MB8479

-> neuB#

--> neuS

6@6t).;6 < M2BB19

=> M12BSB

<- --> exbD mete <-exbB

34

Microbiol. Res. 150 (1995) 1 43

68,50 68,00 1······ ·· 1··· ...... I· . .. I .. .. · · ·1 .. ·····1· ·····1··· .. ·· .. 1 .... ··· · ·1 · ···· .. · ·1

168: 5 0 . . I A

U&6h.6t < = < M6349l L220 25

<,==== MS840S

< . s ufI

<-ploc

<-----pare

·1.. .. 1

ii@m. 53 U09l77

- - > - ---> -- > hybA hybC hyb8

--> -- > hybD hybB

-> hy bP

• > hy bG

?secC

69 , 00 I · ·1 I · ·1

W@Sg; . 66 M16194

=L=1=4=7=a 1======> VO 02 74

======

--> ttdA

-> ttdB

?hslL

J01687 => V00346

<-- > ----> ygjD rpsU rpoD

---> dnaG

?hslM ?holN

Icm'I.34 = > H58409

=> L22026

a@Stl,]] X54049

VOI SOS =>

<= M64472 <= X66720

X00016~================= M7 7129

--- > parB

---> --> - > tole ygiC y giB

- > ygiA

- > ygiB

?lpcB

<-ygiD

<ribS

69,00 .. I ·· 1 .. · .. 1 ........ 1· .. ·1

M068.70 '11II6,1II16'gI8-.III;;IIII----<~~======== Z21844

~>

Zl1S85

> gIgS

<------glnB

<-y giF

BEBOg, ';8 M1278a

cca

?weeA

<= L12966

<- bacA

?toc

· 1 .. ·1·· ··· ·1 .. ·· .. · .. 1 ........ ·1·· ·· .. ·1· .. 69,50

T

ICD069 .21 &WSg; .i2 > X52800 U18997 > K00218 X52031

X03228

---> --> 0496 ebgR

< -t110

ebgA

70,00 69,50

I·· · .. ···1· ·1· .. · ··· .. 1· .. · ·····1 .. · · .... ·1· .... · .. · 1 .. ·· .... · 1 .... ·,·· ·1· · ·· .... ·1·

,@gtI.42 U18997

XS2031

X032 2 8

---> ebgA 0477 0783

-- > --> ebgC 0356

44 Microbiol. Res. ISO (1995) 1

----> < -0672 f104

< -f138

M352B O

- - ,. -> --> --> <--- <--0179 0221 032 1 0414 f495 f352

- > - > 0230 0115

<- . f18 3

<-f 62

<-- <-f3BB fB6

0472

?uxaA ?uxaC ?exuR

35

70,00 70,50

1 ··1· · 1·· 1· .. ·· .. .. 1 .. ······ · 1· .. .. .. ··1 .. ·· .. · .. 1 .... · .. ··1· .... ·· .. 1··

BeMUS U18997

> -> 0472 0220

-> 0101

<-flB7

<-- <£275 f150

<========================= X14430

< M23638

<---f406

~=2=13=1=2=====

<-tdcB

-> tdcR

<= D90212

--> --> ->

--> -> 0328 0130

--> 011B

-> 0233

-> 056

<-£425 ~haA

<-- -> tdcA 0186 0263 o127a 099

--> --> o127b 0160

-- > 0157

?gurD

170,50 .. 1 .... .. ... 1 .

a@og;.42 U18997

D90212 <= K01993

X00211 <= L19569 <= M17569

<-f298

· 1· ·

=> M30178 => M32358

<-- <---- <--f18B f746 tdcC 0395

71,00

., .. . ..... . ,··· ... · . ·,.· . ·· .. ·.,·· .. ·····,· .. · · · .. ·1· .... ····

> 054

<-

<-- <--- ---> - - > ---> -> --> -> 02S6 0267

-> 0.251

-- > 0231

--> yhaE f444 0523 0154 04.26 0133

--> 0169

0429

rnpB <---yhaD

<yhaF

-> sohA

<f269

--> 0384

--> 0163

- - > 0158

--> 0290

--> 0194

<--f286

0863

· 1 ·1 .. · · ··1······· · ·1·· .. ·····1··· · ····· ' .. · .. .... 1 .... .. I· 71,50

B866g3.42 U18997

f286 --> 0131

<-- -> <--f346 0186 f167

--> 0.298

----> --> <- <f226 £147

->

--> 0331 0678 0196

-> 0191 0100

<--f174

< <= M5B338 U03750

• «<

<= X00761

«

<========== X00513

<== <============ M59862 J02638

<========= M63288

<--- <--mtr f294

--> <----

<= M14425 <= X01073 <= X13775

<- <-rpsO yhbB

<---- <-- <------0335 deaD pnp yhbA infB

71,50 1...+ .. .. ... I ..

T ··,···· .. .. ·1· · · .. ·· .. 1 .. · .. ·1· .. ·· .. ··1 .. · .. ·· .. 1 .. ·

72,00

Ig8gtg.42 U18997 :==========:===> X00513 M35236

<--nusA

<== M21680 <= M2B401 < KOQ30S

argG

L1296B <= D16463 <= X52B01

<-secG

<= <========== <= X5471B D13267 M9235B

<= => => LQ6494 X5946Q X6BB73

<========= M83138

<-- <- ---> <-- <- -> <- <--folP ftsJ dacB f321 rplu nIp fB9 f183

<-- <--- <-- <---- -> <-- <- <--- <- <-yhbC f547 yhbF ftsH 097 f390 rpmA murZ f129 £260

<-- --> <-greA 0323 f211

?cdsS ?fatA ?dgd ?kba ?azaB

36

MicrobioL Res. 150 (1995) 1 45

17~ : 00 ... . I .... . .. . . I . . . ... . . . I . . . . . .. . . 1 ..... .. .. I ...... . .. 1 . .... . ... 1 . . ...... . 1 . .. .. . ... I .. 72, 50

BWOgU2 U18997

<= <== > = > U12684 013188 M90703 M33743 M3 3737

<========== ~> > 012938

===== X53315 L;2=0;2;5=3==M=3=3=7=4=0==============M74162

Z27094 M1SH7 => M57 612

<- - -->- > - > - > -> <- <-----£269 032S 069 yhbG orf95 npr f242 arcS

-> <-0210 yhbL

--> -> 0325 0131

- - > 0 191

- - > 01B5

---> -- > rpoN ptsN

-> yhbH

--> 02 84 - - > yhbJ

gltB <--f309

-> gltF

0224

72,50 1······ .. · 1 .. · .. .. · ·1·· .. · .. 1· · .. · .. .. 1 .. . 1 . ........ 1 ..... ·) .... ~ .. I. Q I ·

73,00

a@sg;. is U18997 > M74162

> 0224

-- > 0159

-----> <--07 93 f33S

-> 0238

<-- <f154 f 229

<= M20207 <= X03 345

<-nanA

--> <-- <---0375 f 302 f506

<== M69028 <= X050SB

<= X02130

--> <- - < --0 455 .epA rplM

<- <- - <- -f263 aspS rpsI

U15661

- -> 0134

= > M1 7 532

< M24777

<= Y00129 <= U04742

=> X13968

--> - - > argR hhoB

---> <-- - > 0104 hhoA mdh

<--£375

?pog ?htrH

7 3,00 73,50 I .... ···· · 1· .... " .. I .. ",, · .. I· ·· .. ··· ·1 · ·· .... · ·1· ,, · .. .. ·1 .. ··· .... I .... ··· .. I .. · ·· .... Iii"

d@6kY .l2 U18997

<==== <==== X57166 M22055

<= M31792

MB0458

<-- <---- <

Z26847

M83198 = >=> M32214 M3 0953 => X14825

= > M79446 ---> £90 fno 0309 ~~ fill ~hdB mreB f646 f33 accC

<---- <- <--- <--- <--- <-- --> --> - > f655 £90 f481 £460 cafA mreD yhdH fabB 080

<--tnreC panF

? ridA ?adhR ?grpD

73,50 74,00 I· ..... .. · 1 . " ...... I .. "~ .. " I ....... . . 1 ..... . " . I .. " ..... IT'" .... ·1 ........ ·1· .. . Ii' ... 1 ... .

d@6t9 .'; U1B997 => => Z2 6847 J 03816 X57948

M95 784 = > X6239 9

? pl:1llA --> 019 4

- > fis

--> yhdG

M96848

-> --> 059 acrE

acrF <-£220

46 Microbiol. Res. 150 (1995) 1

- > 073

--> o368a

<= V00334

<= 012500

-> <-0252 rrlO

--> --> 0368b

< rrvD

<rrfD

030 5

=> X05963

<= X65946

<= Y00710 X77091

--> <-f176

<-£169

0256 <fss

<aroE

< --smg

37

74,50 74,00 I ......... IO······ · ·I .. .. . ··· · I . .. .. .. .. I .. ~ .. .. . ·I . .. ... .. . I .. .. I····· .. · ·1··· .. ··· ·1· [email protected] U1B997

=> => X65946 X00767 XS2114

X77091

<; 10110195 ~;02=54;3====

<= <= <= X00766 10112432 V00357

<= V00344

=> => <= <.~~~=========; < X77BOO UOB371 X53B44 X01563 M12490

=== X63666

<= V00353

<============== X02613

< --> -> --> <-- < rpmJ

<-- <-rplF

<rplX

<- <- <---rpaC rplB rplC f4B9 £102

<amf

frot fmv -->

mscL rplQ rpsD <-- <-- <--

rplO <- <-

rpsH <-

~iv ~lW ~;J rplN <- <-- <--

yhdM rpcA rpsK rpmD fmu --> fme

--> trkA

?tolM ?prlA ?rimE

74,50 1··· ...... 1····· .. ··1··

'@dt9.t2 U1B997

-- > 0271

---> 0493

--> 0145

;hdN ;;SM <--secY

?nirB

<-rpaB

<-rplR

<rpaN <-rplB

?fipC

<rpaQ

<rpmC <--rplP

rpsS rplD f139

?nek

75,00 ... 1 .. .. .. ... 1 ...

0 ..... 1 ......... 1 ......... 1 ......... 1 . .... .. .. 1 ........ .

=> L2B106

<= 10127176

<= J01690

< 10110459

<; J016B9 <; V00356

<= <= J01691 X65735

<:: <= X00415 J016BB

;> X64592

=> L13261 <= Z21496

<- <-- <-- <- <- <--yheA tufA rpsG f119 f270 £196

----> 0654

--> --> 0398 0169

--> -->-> 0195 0388 hopO --> --> 0327 hopZ

<------ <---- <- <- -> yheB fusA rpsL f244 slyD

?kefB

75,00 1 .... ·····1·· · .. .. 1····

[email protected] U1B997

--- ----> -> f601 0637 072

<-- --> f184b 0340

--> o13B

?eloB

<-bfr

?h?hsl?hslQ ?neaB

<- > f95 slyX

<-£12B

<f601

. . 1 . . 75,50

· .. ·1·········1··· .... ··1 .. · .. ····1 .... · .. ··1 .... ·····1··· .. · .. · Q\l

<= X61919 => J01598

<-

> M6390B ;> M15200

yhfA 0696

crp

«

<= <= M32796 M55429 X14202

~3:'2:=3:=5:=4== ;> X57412

<= K00030 ~;2;83:'6;3===

<--pabA ppiA nirB

<-- <- --> argD fic 0393

M64606

--> nirC

-> nirD

-> 055 ---> 0462

--> < -> ---> cysG 035 f55 yhfC 0347

?qmeC

75,50 76,00 1···· .. ···1··· .. · .. ·1 .. .. ··· · ·1· .. ···· .. 1· .. ······1· ...... ··1 .... · .. ··1 .... · .. ··1·········1 .. ·


-> 0149

--> 0132 -> 0261

-> 0265

<--<f423 f292

<-- <--f361 £130

<--<-f3B7 f228

<-- <--f40B f134

.. <= <= <= V00371 J01600 X03867 <========~~==~~~======= Z19601

< V00370

<; Z2B371

<= X15162

<- <--

<= X80167

< M76389

<-- <--

X02164

yhfB dam <-areB hopQ f146 mrcA

<-- <- <---trpS yhfD damX

<- <-- <-aroK f147 f26B

<-£179

38

Microbiol. Res. 150 (1995) 1 47

76,50 76,00 j ......... j ....... .. j ....... . j. .j •........ j .. ....... j ......... j ......... j ......... j.

BEBge;.4; U18997 > X02164

<= M59823

~:1:2:3':'7:4====

<= J01606

~·':'0:1""6':'5':'6===

X71063

~':'1':'5':'5~8 7:=====

<= M32793

<-- -> <--- ---> <- > -> feoA

-> 078

<bioH

--> 0191 fl86

---0711

0237 -> 0133 -> 0294

f574 pckA

?gntT

ompR 052 <--- --> envZ 0170

----> 0622

-::-----> --> -> feoB yhgA 0243

--> 0437

<malQ

76,50 j .... j . .. • . .. j ... j .......•. j • • •.••• • . j ...•••.•• j .•. '0'" .j ......... j •••.•..•. j .. .

77{00

u@agg, 42 U18997

<= M32793 M24342

<c===== X06791 M13585

<====== X02003

<= V00304

<= M10620

malQ malT <c-----maIP

<-f146

<-f201

<====== <===== ~:0=1=6=1=6==== M54940 J03966 => M21277 ~:1=6=93=1=== <=

V00281 <= M13751

M55989 <= <= M22368 M97226

<= J02616

---> <-- ----> <----- <-- <-0532 glpG glpD glgP glgC glgB

<- <- <-glpR glpE f97 ~~ ~~

<--

77,00 j j ....... . . j ••

IiEBBgU; U1B997

M13751 <= V00262

-> <--

HOB

... j . .. ...... j .. .. ... .. j.

<- --> -> glgB 0197 fl62 f231 0162 0138

<-- <_. <-- <-- --> -> asd fll1 f313 f345 0392 insA

<-- --> f332 insB

?gntR

77,50

... j ... .. .... j ......... j ......... j .... 77: 50 Q «

~::2=8=7 2=2==

U00039

<= X14437

<= X56908

~:=0""5""5=1=6===

~:=1""3=1=4=1=======

-> --> <-- <-- <-094 yhhA ugpE ugpB livF <---- <- <- <-ggt ugpQ ugpA livG

<-- <--ugpC livM

?hydD

j ........ j. .. j .. . ... .. j. . .. j ..... .. .. j ......... j ......... j ... .. .... j ..... .. .. j . 78,00


J05516 <= <

V00301 <=

M13166 K02178 <= <= ~:0:4=3=9=B======= M10427 M10426 <= <= M2937B M29377

<= M2066B

X73143

-> <-- <- --> -----> -> <-- -> --> nikB livM

<-livH

<-livK

yhhK rpoH <--livJ

48 Microbiol. Res. 150 (1995) 1

ftsE yhhG yhhO yhhQ yhhS <-- <--- <-ftsX ftsY yhhM

-> yhhN

<yhhP

-> yhhR

yhhU --> 8004074

---> nikA

-> nikC

-> nikD

nikB

39

1 :8: 00 .... I ... . . . .. . 1. · 1· · ·· ·· ··· 1· ·· · · · · · · 1·· · ··· · ·· 1······ · ·· 1· ·· · · ·· · ·1 ···· ··· · ·1··· 7 8,50

d@ggY .iS U00039 => X73143

= > M2971 7

= > X67639

L02370 > D21140

=> M21762

<-- <-yhhJ yhi H

<- - - < -yhiJ yhiK

--> yhiM

---> pit

-- > uspA

- > yhhG

- - > yhhli > <--- <-- < -

rhsB yhhI ~~ ~hlI yhiL yhiN yhio

yh ip

?strM ?gltN ?suhA

78,50

1····1 .. . . .. 1 . . ....... I .· .. · . · ·· I ·· S······ I· · · ·· ·· · · I · · · · · · ·· · 1· · · · · ··· ·1··· . ····· 1 79,00


<===== = > => <= M93984 M13141

- > o.!---- - > yhiP prlC yhiW

<dinD

?hydC

--> gor

X63987 D11109

= === X80057

- -> arsB

-- > arsG

--> ars F

-> yhis

?tolZ

=== => L23635 Dl1389

<- - > - > yhi D hdeD yh iB

-- > yhiF

<hdeA

<-hdeB

?kdgK

- - - -----> <-yhiV yhiW

--> yhiU

? l euR ?alu

179

: O~ .... I. . ..... 1 ... .. . .. . I . . .. . . . . . 1 . .. . .. ... 1 . . . . ... .. 1 ...... . . . 1 .. .. . . . . . 1 . . . . . . ... 1 .... 79: ~~

HEBge;. 42 U00039

<= M840 24

<-- <- -- - - - - > yhiX yhjA treF

<--gadA

7 9 ,5 0

I · ·1

a@st;. 42 U00039

yhjL ~hjN <--yhj M

. . . . . 1

< - . yhJB

--> ---> < ~ - --> <-- <--- --yhjC yhj B yh j G kdgK dctA yhj L

--> <--- < - <--- <----yhjD yhjF yhjH yhjJ yhjK

?inm

· 1· · · ···· ··1 · ··· 1· I· . . . 1 . · 1· 80,00

---> yhjs

- > yh j T

yhj R yhjU

<========================== L08 399

<= M35045 <= X58051

T «

<= XS2802

<= XD2434

--> <-- <-- <--- <- ---yhj V dppD dppB dppA yhjw

<-- <--dppF dppC

<yhjx

40

Microbiol. Res. 150 (1995) 1 49

BO,50 BO,OO jj . . . j . . . . . j . . . " . ~. ·· .. j ·" .. ·· .. j ,,· .. ····j .. ···6"·j .. " .. · .. j··;

BESot3. 42 U00039

=> => < < J02606 M30139 J01622 K01996 => => <= < X03B45 X07037 J01623 X04691

<====== <= M34B27 86B257

<= XOO772

--> --> <-- - > < --> <-- <--- ---> yhjX tag yiaD yiaF cspA glyS yiaH yiaB xylA xylG

~hjY viae <:----bisc

--> viaE yiaG

<--glyQ

---> IS1SO

<-- --> yiaA xylF xylH

<---xylB

BO,50 j".... j j. .j .... " ... j .. ....... j ..... .... j .. .. ..... j ..... . ... j

B1,00

8E8Stg.!; U00039

===== <= X5B994 X16644

<= Y00490

--> ----> < - - <- ~> -> --> <- <--xylR malS avtA yiaJ viaL yiaO yiaQ yjaH yiaU yiaW yiaY

<- --> --> ---> --> <- <-- <---bax vial yiaK yiaM yiaP yiaR yiaT yiaV yiax

--> yiaN

?nfnA ?rntlC

B1,00 j .. "" ••• j .. .. " ·1· · .. .. 1 " . " .. j" .. j ..... .. .. j ...... . .. j ........ . j ..... .. .. j

91,50

dEBStg.42 U00039

X16644 ~L=1=9=0=4=4=====================================>V01503 =>

U03B45 <= => M64177 M21761

U16247

M29716

<--- <-,- -> .. >

=> X06793

=> X06794 => X51359

L13970

<-- ----> -> --> selB < __ yibF _______ _ yhiR

-> yibG yibH mtlA mtlR

<-- -->

---> lctP

-> -> lctD

selA rhsA

81, 50

I· • • • • j .

kEBdg9.iS U00039 => L13970 <= M34333 <= M15745

-> yibK

<= M244B9

<- ---> seeS yibO

. . • 1 .

<- <- --> cysB yibM yibP --> <--cyaX yibN

<--gpsA

50 Microbiol. Res. 150 (1995) 1

yibJ

Q .j.

<======= X06690

j

yibI mtlD yibL lctR

?rfaM ?pyrs

.. j." .. "j .. .. . . . I . . . . . . . I . . .

=> X54492 ~'~9~5~3~9~B=======

82,00

M33577 ~~B~0~5~9~9=====

<-- <--tdh yibB

<--- <-- --> yibD kbl rfaD

X62530

--> rfaF

---> rfaL

<- <-- <--

--> rfaC

rfaZ rfaJ rfaB <-- <- <--rfaK rfaY rfa!

<-rfaS

?glvC

41

B2,00

I·· ·1· ... ·····1·· Q . . 1 . . .

Q. » . . 1 .

»

B2,50

·1····· ····1····· ·· · 1··· «» :. ::0

1@6gY,42 <=

U00039 X63366 V01578 X14235 => M84400

=> X59550

<= M80599 X06036

~M~8~6~3~0~5=======> ~01677

==== M60670

L10328

<-- --> <- <-rfaS rfaG kdtA fpg rpmB

=>=> XOl714 X72920

--> dut

<= X00781

<- --> pyrE Vice

M15266 M64367

"'J=04=9""7""6====

M24503

-> --> -> yicG gmk spoU

<= X17499 <= D00626

<-- <-- --> --> --> <-- -> <--- -> rfaP rfaQ kdtB rpmG dfp

<--ttk rph yicD yicF rpoZ recG

<--radC spoT gltS

?mutC ?mutM ?pcsA ?sipC ?sipD

83,00 82,50 I· 1

» . . . . 1 . . . . 1 . T ~. ·1·· ... 1 ~~ ...... ) . ~ .... ~. 1 .... Q .. 1

dcBgg9,i; L1032B > X59550

Xl7499

D00626

gltS ----> yicH

> X13994 ~>

X17419 > X1S993

<--- ---> yicJ yieR

<= <========================= M12163 Ml7102

<========================= MB9479

<= J01633

<- <--- <--- <--- <---nlpA yicM yieO uhpT uhpB

---> <----- --> <- -yicN

----> <-- <--VicE yicI yicL yicp

B3,00

uhpC

?mul

uhpA <ilvN

I·.·····~ L······~·i·~····· · ·I .... 1·········1·········1······1·········1·· B3,50

1 . . . . . . . . .

[email protected] LI032B

J01633

<--- --> <-- <;;--- --> iIvB

< ivbL

emrD yidG yidJ < - - - - > yidF yidI

yidL <----yidK

<-glvB

<-glvG

« «Q» »

~:'9=4=1 0:=4===~

-> <--yidp h.ls ~idR ~~

<--- <-E002673 h.1T

--> <----yidS yidU

<- <--- --> <-yidH glvc yidQ

?dgoA ?dgoD ?dgoK ?dgoT ?dgoR

B3,50 1· ~~ ..... ·1· ~iJ· ..... 1 .. ··1 ·····1··· ······1·········1···· ·····1··· c:»:to 0'» »

[email protected] L1032B

<============ < < X04341 M13B22 M12487

<= K02179

< J01602

=> Ml1056

<= S57109

=> Ml1990

=> M59914

=> KOOQ32

yidV

84,00

····1········ , 1········· » ».

<-- <- <----- <-dnaN

-> ---> ---> --> <--yieB yieG yidW yidA gyrB

--> <-yidX yidB

?proT ?yidD

<-reeF

<---dnaA

rpmH -> rnpA ---> yidC

thdF tnaA yidY > ---> --> -> -> tnaL tnaB yidZ yieF yieH

?purP ?trmF ?stsA

42

Microbiol. Res. 150 (1995) 1 51

84,00 1---1· ····1···-

« .. I· .. ...... I ..

.Q

84,50 .. I ........ ~i" .... ~ .. I ... B~' ... I ....... - - I ., ...... .

[email protected]; L10328

<=================== <= < M16487 VOO620 J01594

<====;<= <; < M15746 M17098 M24254 M25464

<=============== < XD2723 X01631

<; <= < <= X04340 V00619 V00267 V00266 <:=================== <=

-> <-- <---yieI yieL

--> B004831

<-yieK

bglB <--yieC

?aroI

<----bglF

K01992 <patB

<phoU

<-patC

<--pstA

<-patS

<----glmS

<---glmU

<-- <--V00264 <-atpH atpC atpG

<---atpD

<---atpA

<-atpF

B4,50 1 .. · .. 1 .... · .. 1

« ·1

85,00 . ~ ... 1 .. ~ ...... I . •.... •.. I· • . ..... iJ I ... •. .... I · ..... ~~ . I ........ .

sEBgeg .• ! L1032B

=> => => X01631

J01594

M25464

V00266

V00264 <

V00263 X6B551 K00511 D10466 M10739 => K00764 KOOB26

J01657 => V0030B =>

M13169

<-X02B20

<-mioe

<-- <-- ----> ---> --> --> <--atpE gidB yieD yieN trkD rheA rhsB rbBR yieP

1

<atpB

<--atpI

?het

85,00

JESStY.4! LI0328

<----gidA

<-asnC

--> asnA

<-yieM

?bglT

1· .... 1 .. · Q «::to

·1 .... · .... 1 .. ·

M32253 ~=3=7=3=3=7========

MB7049 > K00260 > M24301 > M25066

MI0313 => M1l6S1 => V00290

=> X02413

-> --> -> <--- ---> rbsD rbsC rbsK yieO rrsC

rrlC

··1··· .. · .. ·1 .. ·······1 .. · .... ··1 · ········1· Q::t 0 0>>>

85,50

=> K03503

M1l689

X04794 => Mll055

<:======= X56310 J01673

<= => M83316 M10424

=> K02845 => M12779 => M26133

<- -> - - > ----> <-- <--rrlC

85,50

yifD > -> rrfC yifB

<--yifA

<---yifB

ilvL yieH ilvD --> -> ilvG ilvM

--> ilvB

ilvY rep ---> ---> ilvA ilvC

?ridB

tnmrA <--gppA

-> trxA

rho

?rffA ?rffC

L ·1· . . . 1 . . .

» B ·1 »

... 1 . ~T' ..... 1 . ~ ... ~ ... 1 .. TTiJ~ ... 1 .. . .... ~ . 1 Q

86,00 ·1· . . . . . . . .

[email protected] M87049 ;> J01673 L18799

M76129 => S75640

> --> --> -> --> rho yifC rffD yifG yifI rffT

-> --> --> --> --> rfe nfrC rffE yifH yifJ

52 Microbiol. Res. 150 (1995) 1

-> rffM

=> K01994 > XOSB51

> K00211

M90498

---> --> yifK atsB

<---atsA

~717276174~====== <= X13406 J01599

<= X04242

X01653

X66782 <-- <-hemY hemD

<--hem){

<--heme

?fip8

43

86,00 I . . . . . . . I . Q

H88629.42 M87049 => XM232U

X04242 => J01599

X01653

X66782

cyaA

. . I . . ···1· ···· .... 1· . .. I· .. ··1 .. · .. ·· .. 1····· .. ··\··· ..

=> X1296.8========D=00069

M3B257

Q «» »Q :. » »

=> => => Ll1042 X02143 X03155

====== L02122 M30198

===== X00738

-> --> > <-- <-- <-- --> --> dapF xerC uvrD yigl yigF E002625 yigJ yigL

I . . . 86,50

<= M37630

=> J04155 => Z28370

<--metR

<cyaY

-> yigA

-> <--yidP 11002616

<-- -> <-- --> yigK pIelA yigG yigM

?tabC

eMgU2 M87049

<= D211S1

X156B9

--> --> -> metE udp yigO

---> -> yigN yigP

?fexB ?ubiE ?ubiD

87,00 I ......... j

QQ .. I·

n

IE8869.42 => M870L19201

--> <-- ====--> --> corA rarD recQ pidB metB

?bioP

87,00 .. I . . . . .. I ..... ~ .. '1· .... Q' .. I .. ... . ... I . ~~ ...... \ ~ ..... ; . -L .... A' ..

->

<============= <= X65013 JOS498 X54687

=> M85227 => M61182

<===============

--> -> -> yigU E002877 yigY

M74164 < X52837 <--faelA

---->

=> X68660

--> hemG yigR

--> --> 8004355

-> friD

<: ____ pepQ ---> --->

Q

yigS -> -> yigT 8002906

<-rfaH

--> 8002940

?fcsA

I . . . . . ·1 ...... 1· Q «»:.

fadE trkH rrsA

..I ......... I~.

X63186 => M77746

J01663 => J01664

=> X01895

X82073 <==

<=======~K~0;,;2;,;l;:.7;,,6~ XOS173

=> Z11492

----> <-- --> ---> > rrlA yihC yihE yihF polA

<- ---> <---yihA hemN glnG

<= M13746

<= V00282 <= M10421

<--- ----> gInA yihK

> yihD <-- -> --> <--rrfA

<-- -> mob dsbA

87,50 1 ........ ·1 .... .. .. ·1· ...... \. » »

£@664.42 L19201

--> ---> <---yihL yihN yihP

yihG Bpf yihl glnL

1 ........ · 1 .. ··· .. ·1· . . \ . . . . . . . BB,OO I .. · ...... I ........ ·

«

<-- <-- -> -> --> yihR yihT yihV yihX yihZ

» «

<= X16016

- > <--yiill fdol

--> <:----'-yihM yihO ~~

<-- <-- -> --> --> yihS yihU yihW yihY yiiD

-> <-yiiF fdoH

<--fdhll

<--fdoG

?manC ?rimO

44

Microbiol. Res. 150 (1995) 1 53

88,00 I ....... ~~ I . ~ .

a@6iU; L1n01

. . I· ...... ~~ I . . . . I . . . . . «» Q>

< <= X60472 M85158

> X0605B 867716

<= X60699 => M94879

=> X03951

! ......... I ••• . 88,50 » »

<= M36795

=> L14579

--> <-frvX

<-frvA

<--- -> rhaS

<-- --> <--- --> fdoG yiiG

--> fdhD

BB,50

<----frvR

<--- <-frvB yiiL

<rhaD

rhaA rhaT <-- --> rhaB rhaR

kdgT -> sodA

cpxA yiiO -> <-yiiM yiiN

-> cpxR

yiiP

B9,00 I; .... ~ ... I; ...... ~.I~.· ···1·······1··· ... ·1·· n «»0 c 0 ··1·········1·········1···· ·····1······ ... « ¢« « 0« «»

HEBggg.42 119201 <= => X05960 M11331

X02519

<= => => L04?57 M18393 X05961 <= <= M19644

~14281 ~:'3:'3=29:c3:=== <= X03683

<= M12869 V00305 => K01546

<= XOOG17

<= Z11767

M55990 <= X15054

<====== D00616

< X68638

> yiiP --> pfkA

89,00

--> sbp

-> cdh

?menA

<- -> <tpiA yiiR myrA

<- ~ - -)0

yiiQ yiiT --> <--yii8 glpX

?argM

~~ <glpF

-> <--- <-- <-,----- <-- --> yiiU halU ftsN priA yiix metB

<-- <-- <-- -> yiiv hslV cytR rpmE

<-- <-yiiW metJ

metL

?rit

I ....... ~ . I . .. . .... I ......... I . . . . . . . I .. . 89,50 . . I ....... . . I· .. . ..... I ..... . ... I ......... I ...... ~ ..

U2&6Y.B Ll9201 X'=O'=S'='9'='O 3:==== =>=> V00305 V01Sa2

=> K01547

U00006

M2l5H

---> metL katG

89,50

--> metF

?nalD

<= J02632

<= X01700

<- - <- - > --> <- <0------yijF talc yijI frwB pflM yijo ppc

--> <-- <0----- ---> -:---- --> < ___ _ yijE gldA ptsA frwc pflC frwD yijP

I·· ·1· ···1··· ·····1· » Q ··1 , ·· .... . . I· ·13····· .I T·T· ... . · 1·· ...... ·1··· .. · · ··1 90,00

TT

S@ug;.42 {J00006 <= => <= => X62807 J01590 X66026 M10112 X63978 M2039? M10123 JOl7l?

=> => <= => =><=> J01587 J04S53 M5?568 L14556 L145S? M9007l K00275 => J01589

<-argE

H21446

--> argB

=> M34102 => X16531 => X52666 --> <-- --> oxyR udhA yijD

--> ---> -> argC argH

54 Microbiol. Res. 150 (1995) 1

yijC <-trmA

=> ~~:,:,==============> => Xl7416 J01695 M15B20

=> X041?6

V00348

----> ---> ------> --> btuB rreB rr IB murB

<= M36321

=>

<-coaA

X04181

-> murI

> --> <B002670

?rer

rrfB birA --> I81 tufB

45

9 0 .00 I · · ····1· ·· ·· 1 .. I .. · .. · ·· . \ . ... . .. .. \ . ... . .. . . \ . . .. ... .. \ ...... ... \ .

90,50

[email protected] U00006 > =========================================== JOl71VOOJJ9 => M30610

:> --> - - >

<= M38 332

VOOJ40 = > V00341

tufB rpIK rpIJ --> - > - >

MJ8288

rpoC

=> Ml1672

D12624

<-- <- <- - -- -> thiH thiF thiC yjaD yjaF

- - > <- <-- < -- --> se:~ > rplA rpl_L _ _ ____ _ __ here thiG thiB yjaB hemE

nusG rpoB y jaG

?thiB ?thiA ?cpsF 7mrbA ?mrbB ?mrbC ?envP

90,50 I ·.. . .. I· .n n I ·· .. I · · . 1 . . .

91,00 . \ · ··· .. \ ...... · .. 1 .. · ...... 1 .... ·· 1

»

[email protected] U00006 » DX54556

= > > ~·':6'='3=4'='9 7:==== => M22975 => X05994 > X54555

M2B369

-, - - - > hupA hydH

J=0=5='=2=6=====

~=5='=9=5=0=======

<--- ---> ------pu rD rrsB rrlB

X0 0162 X12431 => = > X02BOO M22621

= > M102 10

= > X14501

= > X07543

::M'='2'='0 7=":"4===

M18974 <-y jaB

---> -----> aceS aceK

<= M63914 <= M31 7 61

< --iclR

- > - - > <- - - > rrfB - ->

---> <;----yjaH hydG

< --yj aI

91,00 1 • • • • . • • . . 1

b@Sk;.4; UOOOO6

M63914

M31761 = > M650 79

J04975

X16584

iclR

metH

91 , 50

\\ n

U00006

J01648 > ===== V003V00298

> VD0297

--> malK

--> lamB

= > X04477

- > malM

purH

yj aA --> metA

aceA yjaC

.. · 1 .. ·· .. · · · 1· .. .. ·· · ·1······· ·· 1 .. .. .. · .. \ .. ·· .. .. ·1 .. . ···· .. 1· · · 1 91,50

.. \ .

<= MU812

<= XOOOOB

X15196

<=:=:===== J02812 J01648

= > <= MJ3742 X02871

<= X0666 3

<---

VOOJ03

yjbE yjb;

yjbH -> psiS malG

<-malE

<--- <---xylE malF

-> yjbG

..... \ . . ·· .. · \ · .... .. .. 1· ··· ·1 .. .. .... ·1· .. · .... \ · · ···· · · · 1· 9 2, 00

X66619 => M93136 => M93413 => X57434

=> XK00127 --> ubiA

= > J01643

L02362

d~kA yjbJ yj~ - - > ubiC

<------ --- > <---pIsB lexA yjbK

--- > -> dinF yjbL

?tnm

< ==> L02312 M12047

= > = > K01174 X0362B

= > M17B09

<=

--> ---> yjbo dnaB tyrB

--> <-- --> yj bN qor aIr

?g l t R

Z26592

<-EG02597

hobH

46

MicrobioI. Res. 150 (1995) 1 55

9 2 ,50 92 ,00 I ·· · · · · ··· 1· · · 1·, · ·· · · ·, 1· · ·· ·· ·· ·1 .. · ·· ·1 · · · · .. ··· 1· · ·· .. ·· ·1 .. .. · · .. · 1· .. .... · · 1· .. ·

MOh .n U0000 6

= > Z2 6592 X01 62 1

<====== M13 495

<= J 0172 1

= > J 01 70 4

= > X59593

= > M6 0111

= > X722 98

> - - > ---> -> <- - - <---- ---> -- > hobH s s b y j cC s o xR yjeB yj c G acs nrfA nrfC --> E00288 5

- -> y j bR

<------uvrA

?aidC

< -yjeB

< - --- > <--s oxS yjcD y j e F

?bymA

<--y jeH

- > nrfB

92,50 I .. ··· 1· · · · · .. ·· 1· · ·1 .. .. · · .. ·1 .. .. .... · 1 .. ·· .. .. · 1· . . · 1···· ··· 1

liMb. i2 U00006

= > M3 2 488 = > M848 05

- > nrfF

- -> nrfG --> gltP

<====== M13 563 U14 00 3

<- <--- <-- <- - - - <--- <--yj co y j eP yj eR yj e S yhiH yj c W f3 07

<----- <--- - <-- <-- <--fdh P yjcQ yjcT y jcV f311

nrfB --> nrfO

?mgt

93,00

<======= 090227

<===== J OS26 0

- - > y j eA

< - phnQ <ph nP

< -phnM

93 ,00 I ·· ··· · ·· · 1· ·· ··· · · · 1··· · · ·· · . I . . . . . .. ~) . . ; . . .. . · 1· · · · ·· ·· · 1· . ·1 ·· ·· · .. · .I .. .... ~ .. I Q

93 , 50

kEndlg. 42 U14 003

D9 022 7 M83 08 9 <= U02384

= ================================= <====== <= = = = J 05 260

<-

<= M33730

< M3372 8

< - - < - -phnM phnK phnI phnG phnD ph nB y jdA

<- <-phnL phnJ -- > E002983

93 , 50

<phnH

I · · 1···1

DE86g;.42 U1400 3

= > M9336 2 XO. 894

<- <- < --phnB phnC phnA

<-phnP

< -f73

I .. · · 1

M=2=7=0=5=8==== = > = > M18 425

adi

K0 199 1 = > KQ1 490

- - - > me lA

<f20 9

X7 98 8 6

<-- <de uB f2 3 9

014 055 M9 33 62

---> <- <-- < -pro P baeR f12 2 a diy

--> 0 281

<---adi

. I ~·~··· ·· · I ···~·· ·· · I Q· .. ·· .. ·I .· 94 , 00

. . I . . . . . . . . . < T

<= ===== H3 06 30

- > 0 76

<= X165 42

<= 0 11104

<---l y sU

<=== ===== = <= M76411 M580 25

M=6=7=4=S=2== = =====

<--- -cadA

<- - c adC

< X5 3235 <= S67 565

<= X77 70 7

<-- - - - > <--- <--- - > <--- <--- < -melR me lB fumE

56 Microbiol. Res. 150 (1995) 1

f543 09 0a

?dgkR

- > 09 8

f485 cadB y jdC

47

94, 00 1·········1 . . . . . . I . . . .

Q«B «» « «

IlEam. 42 U14003

X77707 < X00912

<-

<====== X04066

<= X79BB7 <====== X02307

<= X05967

<--- ->

.. I .

~>

M1041B => M74192

X07B50

. ·1· Q

=> M11294

->

94,50 .... ·1· .~ ..... ·1· 0···· O~ ·1··· ..... ·1· . . . I . . . . . . . .

»

=> <================= X61676 J01611

=> <= => X69949 V00277 XS99BB

<-- - - >

<= M1l979 <= M24174

<- <----yjdC cycY aspA 0125

-> mopA 0128 f342 sugH frdD frdA

---> 0514

<----cycZ

<---deuA

---> mopB

?hslW

<-f289

-> efp

<-- <--ampC frdC

<frdB

--> yjeA

?ssaH

-> ol04a

--> ol04b

<-fll07

94,50 1 ...... · .. 1·· . . . I . . ···1···· .. ·· ·1 · ··· ·····1········· \ ·· . ~ .. ·I .. ·~

95,00 . . I . . . . . . .

TT »

a@og;.!; U14003

<= => => J03916 X53236 L19346 M63655 J04199

> J01624 Z1l831

> => K01549 DOO743

UOOO05

<-- <-- --> ----> -> --> -> fll07 f337 f379 yjeB mutL hfq hflK B002385 0243

<-- -> ---> ---> --> --> --> ---> pad o204a yjeF amiB miaA hflX hflC purA

?poxA ?abs

95,00 1· ........ 1 .. ·

Q

llC86h.42 U14003

-> 0243 0219

?thdc

·1 .. · ·

L20915

--> 0387

I· .... I

<flOO

<-f251

-> --> 0232 0132

-> 0212

---> <- <--aidS f142

. I ......... I . ct:)o Q»

IE66lY.4; U14003

024

<===== M13464

M55170 <= X5400B => M80795

<---- --> 0165 cpdB

-> 0249

I·

<= D21142

-> 081

f356

<= M89992

pms

-> yjeB

??vacB

.. I .. . ...... I ......... I ......... I ......... I .... ~:::O Q :. Q ¢:t «» Q 4:

X04022

---> -> -> -> 0488 0216 o228a priB

--> 0108

---> 0470

-> --> <- -> <--0101 0284 f91 rpsR f224

--> --> <-- -> 0158 rpaF f264 0259a

~lI

?gnts ?cyCA

<f324

96,00 .. \ .... ... .. I . ........ I·~ .... ~ .. I .. ~ ..... I ...... ~ ..

---> 0492

<= M14018

<= M23550

<ileR

--> 0318

-> 090b

<= X12545

<-- -> cysQ

<f184

----> -----> -> ---> --> 0323 f2B6

--> 0163

0577 <---f447

0761 chpBI --> 0116

<-ppa

o417a --> 0341

0457

48

Microbiol. Res. 150 (1995) 1 57

96 , 00 I · · ···· 1 ···· · · · ·1·· · · · 1· · ·· · · · ··1 · ·

d@BgY .42 U14003

<= M95096

= > X67290

<================ U06195

= > D211S7

= > XS4152

L=0=6=O=9=7======== <= U07790

I . . ~ .. . . ·1· I . . . . . I . . 96 , 50

<= <= KOl472 J02842 <= <= JOl670 XOO210

<= <= M10743 M24186 <= M22060 <= M60508

--> ---> <: -- <- -tree

<-- ------ <-- <- --> <--0 457 pmbA fl54

<x96 <x968

?corB

96,50

1· Q

iESdtY,:; U14003

- > cybC

<- - - -nrdD

. 1 ........ ) ..

~=0"'S=8=9=1 =======

;=0=34=9 =7 ======

- > 0105

- >

<= L04574 <= Z141S S

~=l ='S 1:=3:=0==== < - -hol e

<- --

. I . .

--> 0 366

treR mgtA <---treB

·1· . . . . I .

<---fSOO

<--

pyrI pyrL 0164 argI <--fB1

- > 0110

<-- <-pyrB f 1 31

<f261a

-- > 0 1 53b ----> 0616

Q ·1· .. ~; .... I . ~T' ..... i· .. .. ~ .. AI .

<-

~"'9=63:=S:=5==== = > X04174 => M10747

<-f97

?sbaA

97,00

<= J01733

0204b pepA 0361 f33 2 f254 - - > - - :>

0136 0111 - - > ol09a

<------valS

97,00 I· .. ·1

IEBStg 42 U14003

J01733

. . . . I . o »4:1>

- - > > 184 0377 0 67

--> 0425

?prp

97,50 1 .... 1

D@Sg;. 42 U14003

<- f124

. . . I .

<- <- <- - -f260 f72 f437

<-f2l0

<f 2 68a

?cup

<--f383

58 Microbiol. Res. 150 (1995) 1

?gntV

. . . I Q

.. I .. .. " n I . . . .. I ..... ~; .. I ..

< f149

1 .. .. .... 1 «

<--184

97,50

<============ M2639 7 ~=6=31=1=S============

0198 -> 0137

<f248

<feeE

<-fece

~=2=O =98=1=====

<----fecA

<- <--f266 f388

<-f3l9

<-feeD

<-

<-fecI

<-fecR

<----feeB f655

<--f262a

· 1 .... .. · .. 1·· .. · · . . . I . . - . . I . .. I 98 , 00

=> = > L077S6 M11774 XS165S

"'X=0=3=9=23====== => L14598 X05672

= > X00981 => M2760 3

-> - > --> <--0338 f326b

<f241

--> fimB fimA fimC fimD

--> -:>

fimE fim! --> fimF

--> fimG

fimH

?uxuR

49

I :~I. ~~ .... I . . . I . . . . .. BeMUS U14003 => => X05672 X034ll

--> --> <--fimH uxuA f276

<-- ---> f477 uxuB

98,50

II .. ··1······· ··1· n

EEBSgg.:; U14003

·· · ·1·· · ··· ··1· ·· · ·····1·· ······1···· ·····1········· 1· « « »

-> <-- <--0133b f153 f392 <- -> 003 o21B

<--f390a

<--f323

<- <--- --> f257 f426

<-f390b

0103 <-- --> f410 0186

98,50

-> 054

99,00 · .. I ····.

n ··1·· · · · ··1 ..... . . I···· · ····L···· · ··I ........ .

=> <==== => X55662 V002 BB

<===== M24927 <====== M5B752

X54l9B <============ X06545

Z290Bl => V00373

---> --> <--- <--- <------ <- ---> <-0521 mcrD merB hsdS hsdR yjiA tsr f26lb

-> ol09b

<-mere

<- <--- --> <- <f192 f132 hsdM mrr f67

99,00 1· . . ..... I· ....... I .. . . . . I .

BEOSgY.42 U14003

<======= J04030

<----f721

?oppB ?dadQ ?mdoB

·1··· .. ·1·· c:Tc: ::.

I . . . . ·1·· ·······1····· .0

<= => => V00363 L04575 S6727B

=> => X06ll7 MB9635

==== 017724

Z26313

=>

<f26Bb

99,50 I . . . . . . . . . ..

X04l5l => X03224

> M5B709 > X03217

<----- <- <- -> <- <-- --> -->-> -> f750 dnaC yjjB 0241 f262b f343b rimI oamY 0211 deoC

<- - <--yjjA dnaT

?murH

99,50 I . ~... .10'0' I·

£@oR;.42 U14003

<f277

. . . I .

--> --> ---> --> <-0225a holD prfC 0357 f2B7 deoA

-> yjjG

. . . . I . . . ~. I . ..... I ........ . I . ........ I .... :~~:~O » Q «» :»Q

=> => X05629

<= => <= => <= X00742

--> deoA

=> M60917

-> deoD

M30784 X63155 <= L27665 <= X03046

<-- --> lplA serB

--> ---> <-amp

---> ems deoB yjjJ

?nadR ?radA

M69185 M94042 Y00538 L340l0 => ========== <= J017l5 M13608 L20873

<= <= M97495 M10044

<--- -> <-- --> ---> -> f579 trpR rob creB creD 046

----> <-- --> ---> <- -> areA 022Bb slt fl73 creA phoM

-> o2l5b

?thdD ?ereC ?ilvR

50

Microbio!. Res. 150 (1995) 1 59

Acknowledgement

We like to thank E Blattner and G . Plunkett III (UW, Madison) for communicating results prior to publication; Kenn Rudd (NCBI, Bethesda) for his unpublished EcoSeq6 listing; Peter and Kate Rice (Sanger Center, Cambridge) for their help in updating; as well as Peter Stoehr (EBI, Cambridge) for constant flow of recent database additions. This work is supported by the Deutsche Forschungsgemeinschaft (Kr 591/7 -1 ).

References

d'Aubenton Carata Y., Brody, E., Thermes, C. (1992): Prediction of Rho-independent Escherichia coli transcription terminators. J. Mol. BioI. 216, 835-858.

Bachmann, B. J. (1990): Linkage map of Escherichia coli K-12, Edition 8. Microbiol. Rev. 54, 130-197.

Bairoch, A., Boeckmann, B. (1993): The SWISS-PROT protein sequence data bank, recent developments. Nucl. Acids Res. 21, 3093 - 3096.

Berlyn, M. B., Letovsky, S. (1992): Genome-related datasets within the E. coli Genetic Stock Center database. Nuel. Acids Res. 20, 6143-6151.

Birkenbihl, R. P., Vielmetter, W. (1989): Complete map of lSI, IS2, IS3, IS4, IS5, IS30, and IS150 locations in Escherichia coli K-12. Mol. Gen. Genet. 220, 147 -153.

Blattner, E, Burland, v., Plunkett III, G., Sofia, H. J., Daniels, D. L. (1993): Analysis of the Escherichia coli genome IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucl. Acids Res. 21, 5408 - 5417.

Burland, v., Plunkett III, G., Daniels, D. L. , Blattner, E (1993) : DNA sequence and analysis of 136 kilo bases of the Escherichia coli genome: Organizational symmetry around the origin of replication. Genomics, 16, 551- 561.

Burland, v., Plunkett III, G., Blattner, E (1994): Analysis of the E. coli genome VI. DNA sequence of the region from 92.8 through 100 minutes. EMBL/GenBank AccNr. U14003.

Daniels, D. L., Plunkett III, G., Burland, v., Blattner, E (1992): Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science 257, 771- 778.

Dimri, G . P., Rudd, K. E., Morgan, M. K., Bayet, H., Ferro-Luzzi Ames, G. (1992): Physical mapping of repetitive extragenic palindromic sequences in Escherichia coli and phylogenetic distribution among Escherichia coli strains and other enteric bacteria. J. Bacteriol. 174,4583 -4593.

Fujita, N., Mori, H., Yura, T. , Ishihama, A. (1994): Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917 -193,643 bp) region. Nucl. Acids Res. 22, 1637 -1639.

Kohara, Y., Akiyama, K., Isono, K. (1987): The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library. Cell SO, 495 - 508.

Kroger, M. (1989): Compilation of DNA sequences of Escherichia coli. N uel. Acids Res. 17 (Suppl.), r283 - 309.

60 Microbiol. Res. 150 (1995) 1

Kroger, M., Wahl, R., Rice, P. (1990): Compilation of DNA sequences of Escherichia coli (Update 1990). Nucl. Acids Res. 18, 2549 - 2587.

Kroger, M., Wahl, R., Rice, P. (1991): Compilation of DNA sequences of Escherichia coli (Update 1991). Nucl. Acids Res. 19, 2023 - 2043.

Kroger, M., Wahl, R., Schachtel, G., Rice, P. (1992): Compilation of DNA sequences of Escherichia coli (Update 1992). Nucl. Acids Res. 20, 2119-2144.

Kroger, M., Wahl, R. , Rice, P. (1993): Compilation of DNA sequences of Escherichia coli (Update 1993). Nucl. Acids Res. 21, 2973 - 3000.

Kunisawa, T., Nakamura, M. , Watanabe, H., Otsuka, J. , Tsugita, A., Yeh, L.-S. L., George, D. G., Barker, W. C. (1990): Escherichia coli K 12 genomic database. Protein Seq. & Data Analysis 3, 157 -162.

Lisser, S., Margalit, M. (1993): Compilation of the E. coli mRNA promoter sequences. Nucl. Acids Res. 21, 1507 -1516

Medigue, C., Viari, A., Henaut, A., Danchin, A. (1991): Escherichia coli molecular genetic map (1500 kbp) update II. Molecular Microbiology 5, 2629 - 2640.

Medigue, C., Viari, A., Henaut, A., Danchin, A. (1993): Colibri - a functional Data Base for the Escherichia coli Genome. Microbiol. Reviews 57, 623 - 654.

Oliver, S., (1994): Back to bases in biology. Nature 368, 14-15.

Plunkett III, G., Burland, v., Daniels, D. L., Blattner, E (1993): Analysis of the E. coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucl. Acids Res. 21, 3391- 3398.

Plunkett III, G., Burland, v., Blattner, E (1994): EMBL/GenBank AccNr. U 18 997.

Richterich, P., Laskey, N., Gryan, G., Jaehn, L., Mintz, L., Robinson, K., Church, G. M. (1993a): EMBL/GenBank AccNr. U00007.

Richterich, P., Laskey, N ., Gryan, G., Jaehn, L., Mintz, L., Robison, K., Church, G. M. (1993 b): EMBL/GenBank AccNr. U00008.

Riley, M., (1993): Functions of the gene products of E. coli. Microbiol. Reviews 57,862-952.

Rudd, K. E. (1992): Alignment of E. coli DNA sequences to a revised, integrated genomic restriction map. In: "A short course in bacterial genetics : A laboratory manual and handbook for Escherichia coli and related bacteria", J . H. Miller (ed.), pp. 2.3 - 2.43 Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

Rudd, K. E., Schneider, T. (1992): Compilation of E. coli ribosome binding sites. In: "A short course in bacterial genetics: A laboratory manual and handbook for Escherichia coli and related bacteria", J. H. Miller (ed.), pp. 17.19 - 17.45 Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

Selkov, E. (1994): personal communication - information available via [email protected]HOV.SU

Sofia, H. J., Burland, v., Daniels, D. L., Plunkett III, G., Blattner, E (1994): Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes. Nucl. Acids Res. 22, 2576 - 2586.

Steinberg, S., Misch, A, Sprinzl, M. (1993): Compilation oftRNA sequences and sequences oftRNA genes. NucI. Acids Res. 21, 3011- 3015.

Swinbanks, D. (1994): Japan's E. coli genome project falling short. Nature 368, 383.

Umeda, M., Othsubo, E. (1989): Mapping of insertion elements IS1, IS2 and IS3 on the Escherichia coli K-12 chromosome. J. Mol. BioI. 208, 601-614.

VanBogelen, R. A, Sanker, P., Clark, R. L., Boga, J. A, Neidhardt, F. C. (1992): The gene protein database

of Escherichia coli,' Edition 5. Electrophoresis 13, 1014-1054.

Wahl, R., Rice, C. M., Rice, P., Kroger, M. (1994): ECD -a totally integrated database of Escherichia coli K 12. NucI. Acids. Res. 22, 3450 - 3455.

Yura, T., Mori, H., Nagai, N., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A (1992): Systematic sequencing of the Escherichia coli genome: analysis of the 0 - 2.4 min region. NucI. Acids Res. 20, 3305-3308.

Microbiol. Res. 150 (1995) 1 61

ECDC — a totally integrated and interactively usable genetic map of Escherichia coli K 12

Documents

Transcript of ECDC — a totally integrated and interactively usable genetic map of Escherichia coli K 12