[Draft] BOT 1 Notes

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    BOT 1 Lecture Notes by F5XS

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    Science is a systematized knowledge derived from observation andexperiment carried on in order to determine the principles underlying what isbeing studied.

    Biology (bios, life + logos, discourse or study) is the branch of sciencethat deals with the study of living organisms; scientific study of life. botany deals with plants zoology deals with animals

    Branches of Botany(Basic Plant Sciences)

    morphology external appearance, formation (phenotype)

    anatomy internal structures & arrangement physiology processes and functions ecology distributions, abundance of plants and their plants and environment

    [plant] geography spatial distribution of plants [plant] paleontology study of plant fossils

    paleontology study of plant fossils

    ethnobotany (economic botany) uses of plants

    Branches of Botany (Applied Plant Sciences) based on the purposes forwhich the plants are grown

    agronomy science and practice of growing field crops forestry managing and harvesting trees for building materials and other

    products

    horticulture growing, processing and marketing fruits, vegetables and

    ornamental plants; landscaping

    Principles of Plant Biology characteristics that unify all living things

    order hierarchical level of organization simple to complex structural organization components of biomolecules

    have genes (hereditary units) metabolism: nothing lives without energy

    energy is the capacity to do work

    metabolism generates energy for growth, survival and reproduction

    growth and development growth is the irreversible increase in size while development is the change

    in the complexity of the organism

    reproduction

    plants give rise to new plants of the same type by either sexual or asexualreproduction

    responsiveness: sensing and responding

    detects specific forms of stimuli (external and internal glands)

    adaptation

    a trait that gives the individual an advantage in survival or reproduction,under a given set of circumstances

    evolution

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    Advances in Plant Science plant biology has changed dramatically over thelast few decades

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    970 Sharp boundaries between biochemistry, genetics, physiology and ecology

    1980

    boundaries between physiology and molecular biology began todisappear

    absolute need to use molecular techniques in physiology andphysiological ecology became evident

    microarrays and gene sequencing have become common techniques

    what is true for plant biology before is no longer valid for plant biologytoday and in the next decades

    molecular tools will further increase in importance

    integrated approach to further plant biology is being realized

    Theories on the Origin of Life

    Biblical Theory

    Spontaneous Generation Theory

    Comet (Panspermia) Theory

    Chemosynthetic Theory

    Chemosynthetic Theory has four stages:1] abiotic synthesis of simple organic molecules (monomers)2] polymerization of monomers3] aggregation of abiotically produced molecules into droplets4] origin of heredity

    Stage 1: Abiotic synthesis of monomers

    proposed by Al Oparin and JBS Haldane in 1920s; conditions of primevalEarth favored chemical reactions that synthesized compounds

    the earth had a reducing atmosphere

    life most likely emerged under high-temperature conditions

    exact conditions are unknown

    ample availability of hydrogen atoms very little oxygen

    high radioactivity and ultraviolet radiation

    lightnings and volcanic eruptions were very frequent

    the Miller-Urey Experiment was the supporting evidence for Oparin andHaldanes hypothesis

    first to show that amino acids and other organic molecules could have beengenerated on a lifeless Earth

    Stage 2: Polymerization of Monomers

    protenoids theory (microspheres: first proteins) proposed by Sidney Fox

    formed in clays

    many surfaces for protein formation

    many positive and negative charges for protein formation

    iron and zinc served as metal catalysts

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    Stage 3: Protobiont formation

    abiotically produced molecules aggregate into droplets

    formed from polypeptides, nucleic acids, carbohydrates

    membrane-bound

    incapable of reproduction but multiplies by budding or fission

    Stage 4: Origin of heredity

    RNA fragments were the first replicating system

    RNA act like enzymes to assemble new RNA molecules called ribozymeswhich might have constituted the primordial self-replicating system Thomas Cech, 1980

    synthesized simple organic molecule that act as a template to producecopies of itself

    nucleic acid genes were preceded by simpler hereditary systems

    Amino Adenosine (AA) + Ester (E) Amino Adenosine Triacid Ester(AATE)

    The Cell Theory

    all living things are composed of one or more cells

    cells are the basic units of structure and function in an organism

    cells come only from preexisting cells

    Cells are diverse both in size, shape and internal organization.

    Cells are small to promote: transport cell volume to surface area ratios favor small size

    control nucleus to cytoplasm consideration metabolic requirements

    All cells have:

    plasma membrane (plasmalemma)

    genetic material

    cytoplasm

    ribosomes

    Fluid Mosaic Model

    conceived by SJ Singer and Garth Nicolson in 1972

    fluid because of its hydrophobic integral components such as lipids and others mosaic because it is made up of many different parts or kinds of

    macromolecules

    plasma membrane is a lipid bilayer

    provides a barrier between internal and external environment of the cell

    allows certain molecules to go through which makes it semi-permeable

    made up of phospholipid bilayer with proteins embedded that allow for neededpassage of macromolecules

    Ribosomes(protein factories)

    sites of protein synthesis

    are made of rRNA and protein

    cells with high rates of protein synthesis have many ribosomes

    Note: The genus Mycoplasma hasthe smallest bacteria that are from0.1m to 1m

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    Cell Types

    prokaryotes eukaryotes

    Genetic Control of the Cell

    nucleus genetic control of the cell chromosomes location of inherited DNA and programs cells and programs

    cells synthesis of proteins

    nucleolus production of ribosomes

    Cell Wall

    structural support

    provides defense against invading pathogens

    provides pathways for communications between cells

    composed of cellulose, pectin, proteins and hemicellulose (glue that hold thecellulose fibers together)

    Growing plant cells produce a primary cell wall which stretches as the cellgrows. A secondary cell wall may then be produced inside the primary cell wall.

    Meristems

    regions of cell division

    main difference between plants and animals

    makes possible to grow a new plant from cutting

    Apical Meristems

    found at the tips of roots and shoots

    increase the length of a plant

    gives rise to three primary meristems

    ground meristem gives rise to ground tissues

    protoderm gives rise to skin coverings procambium gives rise to the plumbing of the plant

    Other Meristems: Lateral Meristems

    secondary growth

    add girth (width) by producing wood and bark

    ApicalMeristem

    Protoderm DermalEpidermis

    ParenchymaPeriderm

    Procambium Vascular

    Xylem

    Vessels

    Tracheids

    ParenchymaFibers

    Phloem

    Sieve tube members

    Companion cells

    Fibers

    Parenchyma

    Ground Meristem

    Parenchyma

    Collenchyma

    SclerenchymaSclerids

    Fibers

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    Other Meristems: Intercalary Meristem

    why grass keeps growing back after you cut it

    grasses and related plants do not have lateral meristems

    intercalary meristems add to the plants length

    Chromista

    photosynthetic

    source of alginates

    Kelp (Alginates)

    viscous substance extracted from kelp

    used in paper production, toothpaste and in ice cream

    Archaeozoa no mitochondriaProtista dumping groundChromista implies pigmentation

    Eubacteria

    could be either photosynthetic or heterotrophic

    with peptidoglycan cell wall

    antibiotic sensitive

    Archaea

    live in extreme environmental conditions

    Protista

    eukaryotes that do not fit in the other three eukaryotic kingdoms

    generally unicellular; some algae are multicellular

    photosynthetic or heterotrophic

    Fungi

    eukaryotic heterotrophs

    absorptive type of nutrition

    Animalia

    eukaryotic multicellular

    ingestive mode of nutrition

    Plantae

    eukaryotic multicellular

    autotrophic/photosynthetic

    Taxonomy is the science of classifying and identifying plants.

    Scientific Names are names understood by all scientists; common namediffers in different parts of the world. Latin is the language usually used forscientific classification.

    A taxonomist is a person who identifies names and classifies organisms.

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    Aristotle classified organisms based on qualities such as shape, color, abilityto do harm and the place where it is found.

    John Ray defined species as organisms capable of interbreeding and

    producing fertile species.

    Karl Von Linne (Carolus Linnaeus) developed binomial classificationscheme wherein two latin words are put together to indicate the genus and thespecies.

    Writing Scientific Names

    the first word is the generic name (genus) and the second word is the specificepithet (species)

    if there are additional words, they indicate the variety or cultivar

    scientific names are followed by author (unitalicized)

    usually italicized or underlined

    The generic name of an organism is placed first and begins with a large letterand has more common with each other than they have with the members of othergroups.

    A species can be defined as a group of organisms that are alike in almostevery feature and consistently produce like organisms.

    The hierarchy of biological classifications eight major taxonomic ranks:Life Domain Kingdom Phylum Class Order Family Genus Species

    Tagalog English Scientific

    SingkamasMexican Turnip

    JicamaPachyrrhizus erosus

    Talong Eggplant Solanum melongenaSigarilyas Winged Beans Psolocarpus tetragonolobus

    Mani Peanuts Arachis hypogaeaSitao String Beans Vigna sisquepedalisBataw Hyacinth Bean Dolichos lablab

    Patani Lima Beans Phaseolus lunatusKundol Winter Gourd Benincasa hispadaPatola Sponge Gourd Luffa acutangulaUpo Bottle Gourd Lagenaria siceraria

    Kalabasa Squash Cucurbita maximaLabanos White Radish Raphanus sativusMustasa Mustard Brassica integrifolaSibuyas Onion Allium cepaKamatis Tomato Lycopersicon esculentumBawang Garlic Allium sativum

    Luya Ginger Zingiber officinaleLinga Sesame Sesamum orientale

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    Junctions are special proteins that connect cells.

    animals

    tight junctions

    adherens junctions

    gap junctions desmosomes

    for plants, plasmodesmata are microscopic openings in the cell wall

    The cell membrane has a unique architectural design

    Two basic types of embedded proteins:

    intrinsic/integral proteins are proteins bound within the plasma membrane extrinsic/peripheral proteins are proteins that do not penetrate the

    membrane

    Membrane proteins based on functions

    transport/transmembrane proteins

    channels are proteins that act as selective pores carriers bind molecule on one side of membrane and release it on the other

    side

    pumps is a term for carriers if transport is active

    enzymes speed up biochemical reactions by lowering activation energy

    receptor proteins transmit information and induce changes marker proteins give cell identity and are important in cell recognition process adhesion and attachment proteins

    adhesion of neighboring cells through intercellular connections or junctions attachment of cytoskeletons

    Diffusion

    higher to lower concentration

    uses kinetic energy of themolecules

    molecules move down aconcentration gradient (downhill)

    will stop once equilibrium isreached

    Transport processes regulated byplasma membrane

    passive

    no energy required

    simple diffusion

    mass/bulk flow

    osmosis

    active

    requires energy coupled channel (symport and

    antiport)

    proton (H+) pump

    phagocytosisNote: Aquaporin is a transmembraneprotein that facilitates the movementof water

    ACTIVE TRANSPORT

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    Diffusion in three types of osmotic environmentPremise: Water is the one that moves freely

    isotonic

    [SO]=[SI]

    water moves into and out of the cell at equal rates net movement of water is zero

    no change in cell volume

    hypotonic

    the solution has a lower concentration of solutes and a higher concentrationof water than inside the cell

    [SO][SI]

    water moves out of the cell

    cell shrinks; cell volume decreases; the cell might undergo plasmolysis,shriveling of the cell

    Endocytosis is a process in which organisms or fragments of organisms areengulfed by cells.

    phagocytosis(Gk. phagein, to eat; cytos, cell)

    cell eating cells engulf large particles (organic matter)

    pinocytosis (Gk. pinein, to eat; cytos, cell)

    cell drinking

    Exocytosis

    wastes or secretions are expelled from the cell using small sacs/vesicles

    vesicles fuse with membrane and ejects contents