Dr Mark Chong National University of Singapore Dept of Obstetrics and Gynaecology.
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Transcript of Dr Mark Chong National University of Singapore Dept of Obstetrics and Gynaecology.
![Page 1: Dr Mark Chong National University of Singapore Dept of Obstetrics and Gynaecology.](https://reader035.fdocuments.in/reader035/viewer/2022062523/5a4d1ae37f8b9ab05997800b/html5/thumbnails/1.jpg)
Dr Mark ChongNational University of Singapore
Dept of Obstetrics and Gynaecology
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2nd most transplanted tissue• 1 million cases annually in US alone
Current treatment:• Autograft: Site morbidity• Allograft: Donor shortage, immune rejection
Solution: Tissue Engineering
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Resorbable scaffolds + osteogenic cells
Mature in bioreactor TisXell System Note:
• Function of bone is primarily structural low hanging fruit
Mesencymal stem cells
(MSC)
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Day 0 Day 14 Day 28
Increased cellular
proliferationIncreased
mineralisationTisXell
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Day 0 Day 14 Day 28
Increased cellular
proliferationIncreased
mineralisationIncreased
viability
LiveDead
TisXell
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TisXell
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Created 7 mm defect Press-fit 8 mm graft
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SS
SS
S
S
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TisXell stimulates bone formation• 5.7 x more mineralisation than static
TisXell generated bone grafts are highly efficacious• Rapid healing of fracture within 3 months vs
non-union
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Minipig model Larger volume, anticipate issues of
vascularisation Introduce endothelial progenitor cells
(EPC) into cellular mix
Mesencymal stem cells
(MSC)
Endothelial Progenitor Cells (EPC)
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TisXell supports co-culture of different cell types
MSCEPC
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18 mm segmental defect in tibia Monitor over 12 months:
• X-ray, CT, angiography
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1 mth
3 mth
fMSC-EPC
1 mth
MSC
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MSC
1 mth
3 mth
fMSC-EPC
1 mth
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6 pigs implanted with TisXell cultured TEBG
All pigs survived; no adverse reaction
Mineralisation and bridging evident at 3 mths in MSC group; 1 mth in fMSC-EPC group
Studies to continue for long-term safety data (12 months)
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TisXell provides a controlled and conducive environment for generating TEBG implant
Potent osteo-stimulatory cues Efficacy demonstrated in rat model Potentially faster bone regeneration
and vascularisation in minipig model Preparatory work with clinicians for
Clinical Phase I trial