Dominance and Allelic Relationships - South Carolina State...
Transcript of Dominance and Allelic Relationships - South Carolina State...
![Page 1: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/1.jpg)
Complete Dominance:
• heterozygote = homozygote
• w.t. allele is haplosufficient
Incomplete Dominance:
• heterozygote intermediate
• w.t. allele is haploinsufficient
Dominance and Allelic Relationships
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Inheritance of incompletely dominant traits follows
Mendelian principles
Petal color in snapdragons:
Red White
RR rr
F1 Pink Rr
F2 1/4 Red RR
1/2 Pink Rr
1/4 White rr
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Figure 6-2 step 3
Homozygous wild types and mutants express wild type
and mutant phenotypes, respectively
+ = wild type
m = null allele
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Figure 6-2 step 3
+ = wild type
m = null allele
Null alleles of
haplosufficient
genes are
recessive
Expression in heterozygotes depends on haplosufficiency
Null alleles of
haploinsufficient
genes may lead
to intermediate
expression or be
dominant
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Haploinsufficiency
Expression
depends on effect
of active allele
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Heterozygosity for
a null allele:
Sled slows down =
incomplete
dominance
Sled stops =
dominant
expression of null
allele
Null alleles of
haploinsufficient genes
may be dominant
Haploinsufficiency
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Haploinsufficiency
Two active
“wheel” alleles
are necessary
Null allele
(missing wheel)
is expressed as
dominant
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Null alleles of haploinsufficient
genes may be dominant
Example: regulatory proteins
Conditions for dominance:
• both wildtype alleles are
necessary for any expression
of phenotype
− high threshold for
phenotypic expression
− no quantitative or
intermediate expression
Wildtype
Mutant
Mutant
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Null alleles of haploinsufficient
genes may be dominant
W.t. allele produces 5 units of an
enzyme, so that
5 units necessary for w.t. phenotype
(low threshold), w.t. allele is
haplosufficient
null = recessive
>5 units necessary for phenotype
(high threshold), w.t. allele is
haploinsufficient
null = dominant
Wildtype
Mutant
Mutant
AA = 10 u, Aa = 5 u, aa = 0 u
AA = 10 u = wt
Aa = 5 u = wt
AA = 10 u = wt
Aa = 5 u = mut
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Review of Haploinsufficiency
Expressed as
incomplete
dominance
Expressed as
dominant
null allele
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ABO blood types are
based on the
presence of different
genetically coded
antigens on the
surface of red blood
cells
IA A antigen
IB B antigen
i no antigen
Codominance: both alleles are expressed
ABO blood types are
examples of
codominance -
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Codominance in Blood Types
alleles: IA = IB > i
Blood Type Genotype
A IA IA or IA i
B IB IB or IB i
AB IA IB only
O i i only
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Babies Parents
O AB x O
A A x O
B A x AB
AB O x O
Match Babies with Parents
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Codominance
Red = CRCR
White = CWCW
Hybrid = CRCW
Monohybrid:
CRCR CWCW
F1 CRCW
F2 ¼ CRCR
½ CRCW
¼ CWCW
Codominant expression of petal pigment
in a Camellia
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Codominance
Red = CRCR
White = CWCW
Hybrid = CRCW
Codominant expression of petal pigment
in a Camellia
Not:
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Expression of dominance depends
on how phenotype is defined, how
allelic variation is expressed
Many traits exhibit complete,
incomplete and codominance
Sickle cell anemia is one of them
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Hb alleles can be separated by electophoresis
Hb alleles are codominant at the protein level
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Expression of Dominance for
Sickle Cell Anemia
Genotype Individual Red Blood
Cell Protein
HbA/HbA normal normal A only
HbA/HbS normal partial sickle A and S
HbS/HbS anemic sickling S only
Expression in:
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W = branching enzyme
produces amylopectin
w = null mutation
produces amylose
Mendel’s peas showed
three forms of dominance
(although Mendel did not
know it)
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Amylopectin
Amylopectin +
Amylose
Amylose
Wrinkled
when dry Round when dry
Starch
Grains
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Expression of Dominance
for Seed Shape
Genotype Seed Starch
Grains Starch
W/W round expanded AP only
W/w round intermediate AP + A
w/w wrinkled shrunken A only
Expression in:
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Dominant mutations often act as
recessive lethals: homozygotes die
Pleiotropy – multiple
effects from one gene
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M+/ M+
M+ / M
M / M
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Inheritance of recessive lethals
Curly Curly 2/3 Curly, 1/3 wildtype
Explanation: All Curly = Cy/Cy+, due to
homozygous (Cy/Cy) lethality:
Cy Cy+
Cy Cy / Cy
dies
Cy / Cy+
Curly
Cy+ Cy / Cy+
Curly
Cy+ / Cy+
Wildtype
2/3 of surviving
offspring are
Curly
1/3 are wildtype
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Other examples of pleiotropy: recessives for which a single mutation has
harmful effects in different tissues
• Aboriginal New Zealanders • single mutation causes both sterility and
respiratory difficulties
• the mutation alters a protein required for
normal function of cilia and flagella
• Northern, Central European descent - cystic
fibrosis • poor respiration and lung disease, sterility,
obstruction of small intestine
• formation of thick mucus due to mutation in
cell membrane channel protein
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Multiple Alleles: A single gene may have
many alleles with different phenotypes
The white eye color gene in Drosophila has different alleles
that resemble each of the monogenic phenotypes shown
above in a different fly species.
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Different alleles
of white eyes (w)
• different
phenotypes
• may be similar
to mutations in
other genes
wcf ≈ sepia wBwx ≈ brown
wc ≈ vermillion
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Mutant 1 Mutant 2 F1 Mutant
Mutations are allelic – same gene
F1
F1 female carries two different mutant
alleles in the same gene and is mutant
wcf/wcf wa/Y wcf/wa
Test for Allelism in Diploids –
same gene or different genes?
“coffee” eyes “apricot” eyes non-wildtype
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Test for Allelism in Diploids –
same gene or different genes?
Mutant 1 Mutant 2 F1 Wildtype
Mutations complement –
different genes
wc/wc; v+/v+ w+/Y; v/v w+/wc; v+/v
F1
F1 is heterozygous for mutations in each
gene and is wildtype
“crimson” eyes “vermilion” eyes wildtype
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ch - an example of
conditional
temperature
sensitive allele
Multiple Alleles in
Mammalian Coat Color:
C = black/brown pigment
cch = chinchilla
ch = himalaya
c = albino
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Conditional
temperature
sensitive
effects on ch
Near body temp
Tyrosinase inactive
No pigment
Below body temp
Tyrosinase active
Dark pigment
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Comparison between
Temperature-Sensitive Conditional,
Genotype X Environment Interaction
Temperature Sensitive
phenotype repeatably
modifiable after
development complete
ex: shts, ch
G x E Interaction
one time effect -
phenotype not modifiable
after development
complete
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Multigene Effects
and Interactions
Genes work in pathways anabolic, catabolic
Most genes affect more
than one character;
more than one gene
affects most characters
Mutations block steps
in pathways, cause
mutant phenotypes
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G. Beadle and E. Tatum
• studied Neurospora (bread mold)
• genetics of biochemical
pathways
• isolated three mutant
strains
• deficient in biochemical
pathway for argenine
synthesis
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Microbe Nutrition and Growth
Wildtype microbes synthesize their own amino
acids and vitamins if the necessary precursors
are available (carbon source, inorganic salts)
• can produce colonies on minimal medium
Microbes with nutritional mutations cannot
synthesize essential nutrients
• cannot produce colonies on minimal
medium
Mutant microbes can produce colonies on
medium supplemented with the missing
essential nutrient
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Mutants and
wildtypes can grow
Only wildtypes can
grow
Microbial Mutant Screen
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G. Beadle and E. Tatum
Isolated three mutations
• blocked synthesis of the
amino acid argenine
• were nonallelic –
assorted independently
B & T tested growth (+/-)
on different potential
intermediates in the
argenine synthesis pathway
• discovered differences
between the mutants
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Potential intermediates in the
argenine synthesis pathway
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Each mutant grew on a different
combination of supplements
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Precursor Orn Cit Arg
arg-1 arg-2 arg-3
Beadle and Tatum’s Interpretation
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Biochemical Pathways: a general model
Precursor (upstream end)
Intermediate
Product (downstream end)
enzyme A gene a+ (or A)
enzyme B gene b+ (or B)
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Biochemical pathways usually have many steps
Precursor
Intermediate 1
Intermediate 2
Intermediate 3
Intermediate 4
Product
enzyme A gene a+
enzyme B gene b+
enzyme C gene c+
enzyme D gene d+
enzyme E gene e+
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Microbiol. Mol. Biol. Rev.
March 2007 71:36-47
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Tryptophan synthesis in prokaryotes:
An example of a multistep biochemical pathway
controlled by different genes
trpF
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Genetic analysis of biochemical
pathways in microbes and prokaryotes
Mutant Sup A Sup B Sup C Sup D Sup E Sup F
Grp 1 + - - + + +
Grp 2 - - - + - +
Grp 3 + - + + + +
Grp 4 - - - + + +
Grp 5 - - - - - +
Five different groups of mutants were isolated. Their growth
patterns on different supplements (intermediates) are shown
below:
Determine the order of the intermediates in the biochemical
pathway and the mutant that controls each step
B C A E D F 3 1 4 2 5
![Page 46: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/46.jpg)
Test for Allelism in Diploids and
Basis for Gene Interaction
Mutant 1 Mutant 2 F1 = Mutant
Mutations are allelic – same gene
Mutant 1 Mutant 2 F1 = Wildtype
Mutations are in different genes
In each case, mutant 1 and mutant 2 may have
the same or different phenotypes
• If mutant 1 and mutant 2 are in different genes and
affect the same character
• Then they may interact to produce a modified 9:3:3:1
ratio in the F2
![Page 47: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/47.jpg)
Mutations are in the
same gene
Mutations are in
different genes
affecting the same
character -
Interaction modifies
9:3:3:1 ratio
![Page 48: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/48.jpg)
![Page 49: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/49.jpg)
w1 w2
A biochemical pathway
for complementary
protein coding genes
![Page 50: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/50.jpg)
Generalized Biochemical Pathway: petal pigmentation as an example
Precursor: no pigment = white
Intermediate: no pigment = white
Product: blue pigment
enzyme A gene a+
enzyme B gene b+
![Page 51: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/51.jpg)
Generalized Biochemical Pathway: effects of mutations
Precursor: no pigment = white
Intermediate: no pigment = white
Phenotype: white
enzyme A gene a
mutant = white
enzyme B gene b+
![Page 52: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/52.jpg)
Generalized Biochemical Pathway: effects of mutations
Precursor: no pigment = white
Intermediate: no pigment = white
Phenotype: white
enzyme A gene a+
enzyme B gene b
mutant = white
![Page 53: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/53.jpg)
Generalized Biochemical Pathway: effects of mutations
Precursor: no pigment = white
Intermediate: no pigment = white
Phenotype: white
enzyme A gene a
enzyme B gene b
![Page 54: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/54.jpg)
AABB aabb or AAbb aaBB
produces AaBb
Independent assortment in the F2
produces (- = indeterminate):
A- B- 3/4 x 3/4 = 9/16
A- bb 3/4 x 1/4 = 3/16
aa B- 1/4 x 3/4 = 3/16
aa bb 1/4 x 1/4 = 1/16
Independent Assortment
![Page 55: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/55.jpg)
AABB aabb or AAbb aaBB
produces AaBb F1
F2 produces:
A- B- = 9/16 9/16 wildtype
A- bb = 3/16
aa B- = 3/16 7/16 mutant
aa bb = 1/16
Complementation: an interaction between
two genes that are both necessary for
expression of a phenotype
Dihybrid Ratios are Modified by
Complementation
![Page 56: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/56.jpg)
9/16 = +/- +/- blue
3/16 = +/- /
3/16 = / +/- white
1/16 = / /
Dihybrid F2
![Page 57: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/57.jpg)
both genes are necessary for expression of
the phenotype
Interaction between a regulatory protein and
its target can also produce complementation:
![Page 58: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/58.jpg)
Figure 6-18 step 4
Interaction between a regulatory gene and its target
![Page 59: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/59.jpg)
Intermediate elements of a biochemical
pathway may have a distinct phenotype
![Page 60: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/60.jpg)
Biochemical Pathway: Petal Pigmentation
Precursor: no phenotype = white
Intermediate: pink pigment
Product: blue pigment
enzyme 1 allele w+
enzyme 2 allele m+
![Page 61: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/61.jpg)
Biochemical Pathway: Petal Pigmentation
Precursor: no phenotype = white
Intermediate: pink pigment
Phenotype: pink
enzyme 1 allele w+
enzyme 2 allele m
mutant = pink
![Page 62: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/62.jpg)
Biochemical Pathway: Petal Pigmentation
Precursor: no phenotype = white
Intermediate: pink pigment
Phenotype: white
enzyme 1 allele w mutant = white
enzyme 2 allele m+
![Page 63: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/63.jpg)
Biochemical Pathway: Petal Pigmentation
Precursor: no phenotype = white
Intermediate: pink pigment
Phenotype: white
enzyme 1 allele w mutant = white
enzyme 2 allele m
mutant = N/A
![Page 64: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/64.jpg)
![Page 65: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/65.jpg)
AABB aabb or AAbb aaBB
produces AaBb F1
F2 produces:
A- B- = 9/16 9/16 wildtype - blue
A- bb = 3/16 3/16 mutant2 - pink
aa B- = 3/16
aa bb = 1/16
Modified Dihybrid Ratio with
Recessive Epistasis
4/16 mutant1 - white
(colorless)
![Page 66: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/66.jpg)
B/-; C/- b/b; C/- -/-; c/c
Recessive epistasis: recessive phenotype for C gene
masks genotypic variation for B gene –
– Expressed gene is epistatic to the masked gene
(C is epistatic to B )
Coat color genes interact epistatically:
B and C genes in mammals
![Page 67: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/67.jpg)
Biochemical Pathway: Black Coat
Precursor: no pigment
Intermediate brown pigment
Phenotype: black coat
enzyme “C” gene C
enzyme “B” gene B
![Page 68: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/68.jpg)
www.adapaproject.org/doggenetics/
blocked in
brown coat
![Page 69: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/69.jpg)
CCBB ccbb or CCbb ccBB black albino brown albino
produces CcBb (black) F1
F2 produces:
C- B- = 9/16 9/16 black
C- bb = 3/16 3/16 brown
cc B- = 3/16
cc bb = 1/16
Modified Dihybrid Ratio with
Recessive Epistasis
4/16 albino
![Page 70: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/70.jpg)
B/-; E/- b/b; E/- -/-; e/e
Coat color genes interact epistatically:
B and E genes in mammals
![Page 71: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/71.jpg)
B/-; E/- b/b; E/- -/-; e/e
Several crosses in which yellow dogs
were mated to brown ones produced all
black in the F1. – What were the genotypes of the parents
and the F1?
– What would be the phenotypic ratio in the
F2?
![Page 72: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/72.jpg)
Foxgloves illustrate different kinds of
gene interactions and modification
of Mendelian ratios
![Page 73: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/73.jpg)
D/-; w/w d/d; w/w -/-; W/-
Dominant Epistasis
M - anthocyanin synthesis
D - modifier of M
W - prevents deposition of pigment
A dominant allele of one gene
masks allelic variation in another
gene
![Page 74: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/74.jpg)
WWDD wwdd or WWdd wwDD
produces WwDd F1
Intercross F1 produces F2 :
W- D- = 9/16
W- dd = 3/16
ww D- = 3/16
ww dd = 1/16
Modified Dihybrid Ratio with
Dominant Epistasis
12/16 white
3/16 purple
1/16 lavender
![Page 75: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/75.jpg)
Recessive Epistasis M - anthocyanin synthesis
D - modifier of M
W - prevents deposition of pigment
M/M; D/D m/m; d/d purple white
F1 = all purple
F2 = 9/16 M/-; D/- 3/16 M/-; d/d
3/16 m/m; D/-
1/16 m/m; d/d white
purple
lavender
![Page 76: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/76.jpg)
Suppression: one gene can suppress
the expression of another gene
• Dominant Suppression
• Dominant suppressor reverses the
dominant (wildtype) expression of
another gene to a mutant expression
• Recessive Suppression
• Recessive suppressor reverses the
recessive (mutant) expression of another
gene to a wildtype expression
• Both are characterized by 13:3 ratios in
the F2
![Page 77: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/77.jpg)
Modified Dihybrid Ratios
with Suppression
Dominant Suppression: M = purple petal exterior, m = white
W = prevents pigment deposition
Partial F2
Genotype
Flower Petal
Phenotype
9/16 W/-; M/- white
3/16 W/-; m/m white
3/16 w/w; M/- purple
1/16 w/w; m/m white
Recessive Suppression: pd+ = wildtype eyes, pd = purple eyes
su = recessive suppressor of pd
Partial F2
Genotype
Eye Color
Phenotype
9/16 pd+/-; su+/- wildtype
3/16 pd+/-; su/su wildtype
3/16 pd/pd; su+/- purple
1/16 pd/pd; su/su wildtype
![Page 78: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/78.jpg)
Modified Dihybrid Ratios
with Suppression
Dominant Suppression: M = purple petal exterior, m = white
W = prevents pigment deposition
Partial F2
Genotype
Flower Petal
Phenotype
9/16 W/-; M/- white
3/16 W/-; m/m white
3/16 w/w; M/- purple
1/16 w/w; m/m white
Recessive Suppression: pd+ = wildtype eyes, pd = purple eyes
su = recessive suppressor of pd
Partial F2
Genotype
Eye Color
Phenotype
9/16 pd+/-; su+/- wildtype
3/16 pd+/-; su/su wildtype
3/16 pd/pd; su+/- purple
1/16 pd/pd; su/su wildtype
![Page 79: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/79.jpg)
Modified Dihybrid Ratios
with Suppression
Dominant Suppression: M = purple petal exterior, m = white
W = prevents pigment deposition
Partial F2
Genotype
Flower Petal
Phenotype
9/16 W/-; M/- white
3/16 W/-; m/m white
3/16 w/w; M/- purple
1/16 w/w; m/m white
Recessive Suppression: pd+ = wildtype eyes, pd = purple eyes
su = recessive suppressor of pd
Partial F2
Genotype
Eye Color
Phenotype
9/16 pd+/-; su+/- wildtype
3/16 pd+/-; su/su wildtype
3/16 pd/pd; su+/- purple
1/16 pd/pd; su/su wildtype
Phenotype modified by suppression
![Page 80: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/80.jpg)
Partial
Genotype
Dominant
Suppression
(3 wt: 13 mut)
Recessive
Suppression
(13 wt: 3 mut)
9 A - ; Su - Mutant Wildtype
3 A - ; su su Wildtype Wildtype
3 a a ; Su - Mutant Mutant
1 a a ; su su Mutant Wildtype
Modified Dihybrid Ratios
with Suppression
Phenotype modified by suppression
![Page 81: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/81.jpg)
Partial
Genotype
Dominant
Suppression
(3 wt: 13 mut)
Recessive
Suppression
(13 wt: 3 mut)
9 A - ; Su - Mutant Wildtype
3 A - ; su su Wildtype Wildtype
3 a a ; Su - Mutant Mutant
1 a a ; su su Mutant Wildtype
Modified Dihybrid Ratios
with Suppression
Phenotype modified by suppression
![Page 82: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/82.jpg)
mostly white
some purple
F2 = 9/16 A/-; B/- 3/16 A/-; b/b
3/16 a/a; B/-
1/16 a/a; b/b
true white true white
A/A; B/B a/a; b/b
F1 = all white
A cross with dominant
suppression
white
A/a; B/b
white
purple
![Page 83: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/83.jpg)
Analysis of Gene Interactions:
If the biochemical mechanism for
the interaction is known, the ratio
of partial genotypes and
phenotypes can be determined
If the phenotypic ratio is known,
the mechanism for the interaction
can be determined
![Page 84: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/84.jpg)
Interactions of genes in pathways:
Complementation
9:7 F2
Recessive Epistasis
9:3:4 F2
![Page 85: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/85.jpg)
disk long,
F1 = all disk
F2 =
32 long 178 sphere 270 disk
![Page 86: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/86.jpg)
Parents F1 F2
red x white red 101 red, 33 white
red x orange red 192 red, 63 orange
orange x white red 272 red, 121 white,
89 orange
Red is normal petal pigmentation,
orange and white are variants found in
nature
![Page 87: Dominance and Allelic Relationships - South Carolina State ...physics.scsu.edu/~dscott/gen/slides/slides4.pdf · • w.t. allele is haplosufficient ... • w.t. allele is haploinsufficient](https://reader038.fdocuments.in/reader038/viewer/2022102822/5ae74e4b7f8b9acc268e7b6e/html5/thumbnails/87.jpg)
Interactions between Phenotypes
of Genes in Different Pathways
Precursor Int Product A
A+B
Precursor Int Product B
Products from two pathways are added together
or superimposed.
This interaction produces a 9:3:3:1 F2 ratio that
includes novel phenotypes not present in
parents or F1.
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o+/-;b+/- o/o;b+/-
o+/-;b/b o/o;b/b
black orange
F1 = camouflage
F2 =
9 camouflage
3 orange
3 black
1 pink (albino)
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brown vermilion
F1 = red (wildtype)
F2 =
9/16 wildtype
3/16 brown
3/16 vermilion
1/16 white
white
wildtype
vermilion
brown
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Interacting mutations affect eye color in Drosophila
Figure 1-9 part 5
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• Multiple genes affect eye color
• Some of the genes interact
• White eyes can be caused by more than one
genotype (w orcn bw)
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Black
Agouti
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B/-; a/a = black
B/-; A/- = agouti
b/b; A/- = cinnamon
b/b; a/a = brown
black cinnamon
F1 = all agouti
F2 = 9 agouti
3 black
3 cinnamon
1 brown
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Modifier Genes (D gene)
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Chestnut
Palomino
Cremello
Buckskin
Bay
Perlino
Chestnut
Series
Bay Series
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Mammalian Coat Color Genes
A gene: yellow band present or absent
B gene: black vs. brown pigment
C gene: presence or absence of pigment
D gene: modifier, incomplete
dominance
E gene: deposition of pigment in fur
S gene: SP = white patches
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Human Eye Color Variation
blue – brown polymorphism:
HERC2 regulatory gene effects
expression of the OCA2 gene in iris
– brown = full OCA2 expression
– blue = very low OCA2 expression
blue/gray variation due to different
light scattering by the iris
– there are no blue, gray (or green)
pigments
albino: mutant OCA2 gene (no
pigment expression, not limited
to eyes)
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HERC2 regulatory gene effects expression of the
OCA2 gene in iris brown = full OCA2 expression
blue = very low OCA2 expression
both genes are necessary for expression of the
melanin pigments in iris
Interaction between HERC2 and OCA2
HERC2 OCA2
melanin
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Human Eye Color Variation
brown polymorphisms (dark brown
to green) controlled by interactions
among 16 genes controlling pigment
deposition, including HERC2 and
different alleles of OCA2
– green = light brown + scattering (like
blue)
– hazel = light brown + different
pigmentation patterns within the iris
a ring of pigment in the iris can
cause apparent eye color changes as
the pupil dilates or in different light
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